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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Very Low Density Lipoprotein Receptor VLDLR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Larvae were anesthetized and mounted in 1% low-melting agarose on a 35-mm-diameter glass-base dish (Asahi Techno Glass or Thermo Scientific Nunc), as previously described (Fukuhara et al. ...
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bioRxiv - Cancer Biology 2021Quote: Both cell line and patient-derived cells were initially passaged in 2D before they were propagated as neurospheres (GSC) in ultra-low attachment plates with serum-free Neurobasal™ Medium (NBM; 21103049; Gibco, USA) with 10 ng/ml of EGF (PHG0311 ...
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bioRxiv - Developmental Biology 2019Quote: ... Amputated animals were again incubated in carboxy-H2DCFDA for 15 minutes before immobilisation in 2% low melting point (LMP) agarose (Invitrogen, 16520-050). Imaging of the samples was performed on 30 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Porcine adipose derived stem cells were expanded at 37°C and 5% CO2 in normal mesenchymal stem cell growth media (low glucose DMEM, 10% mesenchymal stem cell fetal bovine serum (Gibco, Waltham, MA), and 1% antibiotic-antimycotic (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: ... To induce differentiation the cells were seeded into 24-well plates at a density of 2.5 x 104 cells/well and then shifted to DMEM with low glucose (1 g/L; Gibco, Life Technologies, cat. # 11885076) with the same FCS concentration and supplements used for BM-derived SSC differentiation.
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bioRxiv - Genomics 2021Quote: ... We transduced the cells in biological triplicate with gRNA lentiviruses at a low MOI and after 24 hours selected for cells with gRNAs using puromycin (1 ug/uL, Thermo Fisher A1113803). We harvested the cells 10 days after transduction and extracted RNA using TRIzol (ThermoFisher 15596026) ...
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bioRxiv - Genetics 2020Quote: ... E14 mESCs were trypsinized and plated on low-attachment dish at 5×106 per 10cm in mESC media without LIF: Knockout DMEM (ThermoFisher Scientific, 10829018), 15% Embryomax FBS (MilliporeSigma ...
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bioRxiv - Cancer Biology 2020Quote: ... KTB21 cells were cultured in epithelial cell growth medium (DMEM (low glucose):Ham’s F12 (1:3) medium supplemented with 5% FBS (Thermo Fisher Scientific, USA), 0.4μL/mL hydrocortisone (Sigma ...
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bioRxiv - Genomics 2020Quote: ... followed by a second size selection on a UltraPure™ Low Melting Point Agarose (LMP-Agarose gel; Thermo Fisher Scientific, Massachusetts, USA), removing fragments smaller than 300bp and those larger than 400bp ...
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bioRxiv - Cancer Biology 2021Quote: ... MSCs were maintained in complete media consisting of low glucose Dulbecco’s modified eagles medium (DMEM) (Himedia, Cat # AL006A) supplemented with 16% foetal bovine serum (FBS, Thermo Scientific, Cat # 12662029), 1% penicillin-streptomycin (Pen-strep ...
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bioRxiv - Microbiology 2020Quote: ... The protein was then reacted at 20°C in low light conditions with a 20x molar excess of Alexa Fluor 488 C5 maleimide (ThermoFisher, 540 μM), which was added step-wise over a period of 1.5 h using a 10 mM stock in anhydrous DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... mature organoids (day 35-45) were washed (PBS without Ca2+ and Mg2+) and embedded in 3 % low-melting-point agarose (Thermo Fisher Scientific) at 40 °C in reusable silicone molds (1 cm³) ...
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bioRxiv - Genomics 2020Quote: ... 5 μL of primer mixture (forward and backward primers) (2 μM) and 10 μL of the Absolute Blue qPCR SYBR Green Low ROX Mix (ThermoFisher Scientific, AB4322B). The conditions of the PCR reaction were the following ...
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bioRxiv - Genetics 2020Quote: ... Confocal microscopy was carried out embedding the 6 dpf larvae in 1 % (w/v in E2 medium containing tricaine) low-melting agarose (Fisher Scientific, USA) in a 50-mm glass bottom dish (MatTek ...
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bioRxiv - Neuroscience 2022Quote: ... Real time PCR was performed using SYBR Green mix (#RR820B) with low ROX as the reference dye in 7500RT PCR system (Applied Biosystems, USA). Expression of the housekeeping gene Gapdh was set as control and the relative fold change for the gene expression was calculated.
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bioRxiv - Developmental Biology 2022Quote: ... the whole mount samples were microdissected to keep the area of interest and were mounted in 1.5% low melting temperature agarose (Fisher Scientific, Hampton, NH) in a glass capillary with matching piston rod (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... in RPMI 1640 containing 10 % (v/v) low endotoxin fetal bovine serum (FBS, BioTech, Prague, Czech Republic) and 1% penicillin-streptomycin (Gibco, Dublin, Ireland) and pretreated with 10 ng/ml of soluble anti-CD28 antibody (ExBio ...
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bioRxiv - Neuroscience 2022Quote: HEK293-T cells of low passage (<25) were kept in Dulbecco′s Modified Eagle′s Medium (DMEM, Thermo Fisher Scientific #31966-047) containing 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2022Quote: ... Myogenic differentiation was initiated by switching to low serum Differentiation Medium (DM: DMEM supplemented with 2% horse serum and 1% Antibiotic-Antimycotic, all Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: 6-7 dpf transgenic Tg(vmat2:GFP) or Tg(vsx2:GAL4;UAS:GCaMP6s) larvae were embedded in 2.5% low melting point agarose (Invitrogen, Thermo Fisher Scientific, USA) in a 35 mm Petri dish ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were seeded at a density of 1*106 cells per scaffold and seeding was performed dynamically [31] for 6 hours in 50 ml tubes on an orbital shaker at 150 RPM in osteogenic control medium (low glucose-DMEM (lg-DMEM, 22320, Thermo Fisher Scientific), 10% FBS (SFBS ...
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bioRxiv - Developmental Biology 2022Quote: ... NC cells were aggregated into 3D spheroids (5 million cells/well) in Ultra Low Attachment 6-well culture plates (Fisher Scientific, 3471) and cultured in Neurobasal (NB ...
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bioRxiv - Pathology 2024Quote: ... The adventitial membrane was chopped to the size of 1-2mm2 and placed directly into serum-free low-glycemic medium containing 2mg/ml type II collagenase (Cat#17101015, Thermo Fisher Scientific) for digestion for about 4 hours ...
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bioRxiv - Cell Biology 2023Quote: HepG2 cells were grown and maintained in complete medium containing low glucose Minimum Essential Medium (MEM) (Himedia #AL047S) supplemented with 10% Fetal Bovine Serum (Gibco #10270-106), 1X Penicillin-Streptomycin (Gibco #15140-122) ...
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bioRxiv - Cancer Biology 2023Quote: 4T1 cells were plated at 2×104 cells per well in 200 uL of 7-day post-RT 3T3 CM supplemented with either 50 nM AZD3965 or an equivalent volume of DMSO (control) in a Nunclon Sphera ultra-low attachment 96-well U-bottom plate (Thermo Scientific 174929), allowing the cells to form spheroids ...
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bioRxiv - Molecular Biology 2024Quote: ... a 20μl system of ChamQ SYBR Master Mix (Low ROX Premixed) (Q331, Vazyme, China) is employed on the ABI QuantStudio5 System(Thermo Fisher, USA). GAPDH is chosen as the internal reference gene ...
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bioRxiv - Microbiology 2024Quote: ... The Tecnai F20 was operated at 200 kV and images were acquired under low-dose conditions using the software EPU (Thermo Fisher Scientific) and a direct detector Falcon II (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... BTSCs were recovered from cryopreservation in 10% DMSO and cultured in low attachment flasks as spheres in DMEM/F12 media (Fisher Scientific, #11530566) supplemented with 2 mM GlutaMAX (ThermoFischer ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were end-repaired with Enzymatics low concentration end repair mix (Y9140-LC-L) and A-tailed with Recombinant Taq (Life Technologies, 100021276). Y-Adapters were pre-annealed by incubating a MiSeq Common Adapter with Adapters (A01-A10 ...
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bioRxiv - Genomics 2024Quote: Human dermal fibroblasts (ATCC PCS-201-010) were maintained at 37°C with 5% CO2 and 5% O2 using low glucose DMEM (ThermoFisher 11885-084) enriched with 15% FBS (GenClone 25-550 ...
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bioRxiv - Plant Biology 2023Quote: ... Final quantification of each library was determined according to Illumina qPCR guide with a KAPA SYBR Fast Mastermix Low ROX (Peqlab, Germany) in a QuantStudio 5 real-time PCR system (Applied Biosystems, USA). Final libraries were normalized with elution buffer (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... grids were clipped into autogrid support rings with a cut-out that allows access to the ion beam at low angle (Thermo Fisher Scientific) and stored in liquid nitrogen until being used for FIB milling.
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bioRxiv - Cell Biology 2023Quote: H69 cholangiocytes were cultured with 1.6 μg/ml of the rOv-TSP-2 and rOv-TSP-3 in a low nutrient media for 24 or 48 hours then cells were preserved in Trizol reagent (Invitrogen, Life technologies) and stored at -80 °C until further processing ...
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bioRxiv - Cell Biology 2023Quote: ... cells were supplemented with either DMSO (PanReac AppliChem; #A3672) or different metabolites: formic acid (low dose 0.25 mM, high dose 1 mM, Thermo Fisher Scientific; #A11750), folate (low dose 0.125 μM ...
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bioRxiv - Neuroscience 2023Quote: Larval zebrafish at 4–7 days post-fertilization were anesthetized (0.025% MS-222, Syndel) and mounted in 2% low melting point agarose (Thermo Fisher Scientific 16520), dorsal-up ...
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bioRxiv - Cancer Biology 2023Quote: ... were seeded either alone or with TNBC-derived CAFs at a 1:1 ratio on low adherence 96-well round bottom plates (Thermo Fisher # 174925), in DMEM+10%FBS media ...
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bioRxiv - Cancer Biology 2023Quote: ... Both Brca2+/+ and Brca2-/- cell lines were cultured under low oxygen conditions (3% O2, 5% CO2, 37°C) using DMEM (GIBCO; 41966-029) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: Mouse-derived MPNST cell lines described previously (33) were cultured at 37 °C in a humidified atmosphere in 5% CO2 in low pH DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... The patient-derived low-passage MM cell lines (a gift from G.-E. Ghanem) were grown in F-10 (Gibco BRL Invitrogen), supplemented with 10% FBS (Gibco BRL Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... samples (diluted in 2x Novex TBE-Urea sample buffer) and DNA ladders (Low Range and 100 bp or 1 kb Plus, Thermo Fisher Scientific) were boiled at 95°C for 5 minutes then were run on 10-15% TBE-Urea gels for 30-60 minutes at 70°C using a circulating water bath hooked up to the gel cassette ...
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bioRxiv - Systems Biology 2023Quote: ... ABE8e was expressed as an 8xHis tagged protein from a rhamnose-inducible promoter in BL21-Star DE3 cells with low RNase activity (Thermo Fisher Scientific). At an OD600 of ∼0.8 ...
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bioRxiv - Neuroscience 2023Quote: ... the plates were brought to room temperature and the compounds diluted 666 times by adding 150 µL of low glucose medium with the multidrop combi dispenser (Thermo Scientific, #5840300). Using a 96-well head Selma pipettor (Cybio ...
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bioRxiv - Neuroscience 2023Quote: ... The digestion solution was then replaced with 1 ml HABG (97.75% Hibernate A Low Fluorescence [BrainBits, HALF], 2% B27 supplement [Thermo Fisher Scientific, 17504044], 0.25% GlutaMAX [Thermo Fisher Scientific, 35050061]). The digested tissues were triturated multiple times to mechanically dissociate the cells by using fire-polished glass pipettes with decreasing tip diameters ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... 3D classification using a low pass filtered 3D reconstruction from a small dataset (not shown) on a Glacios TEM (Thermo Fisher Scientific) as a reference ...
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bioRxiv - Immunology 2024Quote: ... both cell types were resuspended in an assay medium (cDMEMlow-Glu) which contained low-glucose (1 g/L) DMEM (cat. no. 31885023, Gibco, Thermo Fisher Scientific), 10% h.i ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were kept overnight in the same fixative at 4°C and then embedded in agarose low melt (Thermo Fisher Scientific®) to be cut in 50 μm sections using a vibratome ...
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bioRxiv - Genomics 2024Quote: ... P individuals were transferred to low-DNA-binding tubes containing DNA isolation buffer (digestion solution from the MagJET gDNA Kit, Thermo Fisher Scientific) and stored at -20°C for subsequent DNA extraction ...
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bioRxiv - Cell Biology 2020Quote: ... Dilutions of primary antibody and secondary antibody (ThermoFisher) incubation varied depending on experiments ...
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bioRxiv - Neuroscience 2021Quote: ... All secondary antibodies were Alexa Fluor antibodies (Invitrogen) used at 2 μg/mL.