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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Rat Golgi phosphoprotein 3 GOLPH3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The fragment was labeled with biotin using the Pierce™ RNA 3′ End Desthiobiotinylation Kit (Thermo Fisher Scientific), and RNA pull-down was performed using the Magnetic RNA Protein Pull-Down Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the product was biotinylated using the Pierce RNA 3’ End Desthiobiotinylation Kit (Thermo Fisher Scientific, Waltham, MA). Biotinylated lncov1 fragments were linked to spherical beads of the Pierce Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific) ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... Caspase levels were measured using the EnzChek™ Caspase-3 Assay Kit Z-DEVD-AMC substrate (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The bands were detected by using SuperSignal pico/femto chemiluminescence kits (Thermo Scientific; #’s1859022/3 and 1859674/5).
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bioRxiv - Molecular Biology 2021Quote: 3 µl of purified RNA were reverse-transcribed by using TaqMan™ MicroRNA Reverse Transcription Kit (Applied Biosystems) and a pool of eight specific stem-loop primers (miR-K1 ...
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bioRxiv - Developmental Biology 2019Quote: ... 3’ rapid amplification of cDNA ends (RACE) was performed for wnt1 using the GeneRacer Kit (Thermo Fisher Scientific). PCR products were cloned into the pCR4-TOPO vector (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... 50 pmol of in vitro transcribed RNA was biotinylated using RNA 3’ End biotinylation Kit (Thermo Fisher Scientific) overnight at 16°C ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was isolated using the Invitrogen GeneCatcher gDNA 3–10 ml Blood Kit (Life Technologies, Carlsbad, CA) or Gentra PureGene (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid concentrations were determined using the Qubit dsDNA broad range assay kit with a Qubit 3 fluorometer (Invitrogen). In vitro myTXTL reactions were prepared by combining 5 nM of pSLP15 ...
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bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Calcium imaging was performed on EROS-/- and control cells using the Rhod-3-AM calcium imaging kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The libraries were sequenced on the Ion Proton platform using Ion PI Chip Kit v.3 (Life Technologies).
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bioRxiv - Genetics 2022Quote: ... we performed ligation between 3’UTR Mp fragments and the vector using the T4 DNA ligase kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples from 3 biological replicates underwent DNase treatment using DNA-free DNA removal Kit (Cat #AM1906, ThermoFisher) and quantified using the NanoDrop 2000 ...
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bioRxiv - Microbiology 2023Quote: ... the synthesized RNA was purified and biotin-labeled using Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher Scientific). The obtained biotin-labeled RNA was used as an RNA probe ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein concentration was measured using the Qubit Protein Assay Kit and Qubit 3 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: Calu-3 and HLFC viability were assessed using the LIVE/DEAD™ cell imaging kit (Invitrogen™, R37601). All fluorescent images were acquired using an EVOS™ M7000 microscope (Invitrogen™ ...
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bioRxiv - Microbiology 2023Quote: ... 3’UTRs were in vitro transcribed from 500ng of plasmid DNA using MEGAscript T7 Transcription Kit (Invitrogen, USA). RNA was purified by LiCl precipitation and examined by electrophoresis in a 1% denaturing agarose gel ...
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bioRxiv - Cell Biology 2022Quote: Live/Dead™ Cell Imaging Kit and CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) were used for detection of cell apoptosis based on the manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The mRNA from plasmid #3 was transcribed using the mMESSAGE mMACHINE T7 Transcription Kit (Thermo Fisher Scientific, AM1344) and purified with the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... rtf1-e3 gRNA (target sequence: 5’-GGAAGAAGGGGAAACCGAGCAA-3’) was synthesized using the MEGAshortscript T7 Transcription Kit (ThermoFisher Scientific) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... gDNA concentrations were measured using the Qubit 3 Fluorometer and the Qubit dsDNA High Sensitivity assay kit (Invitrogen). Input gDNA or cDNA was added to the qPCR master mix containing 1X Colorless GoTaq® Flexi Buffer (Promega ...
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bioRxiv - Cell Biology 2023Quote: The full length Aff3ir mRNA was cloned using commercially available 5’-RACE and 3’-RACE kits (ThermoFisher Scientific) according to the protocols provided ...
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bioRxiv - Microbiology 2024Quote: ... we followed the vendor supplied protocol of the His-Pur Ni-NTA Purification Kit (3 ml, Thermo Fisher). The flow through ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... with a monoclonal antibody against B5 (19C2, rat, 1:1000; (Schmelz et al. 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer) prior to permeabilization of the cells with detergent ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were stained with a monoclonal antibody against B5 (19C2, rat, 1:1000 (Schmelz et al, 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer). Cells were then permeabilized with 0.1% Triton-X/PBS for 3 min ...
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bioRxiv - Genomics 2020Quote: ... Day 3 SP34 (Invitrogen) with 5 ng/ml BMP4 ...
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bioRxiv - Neuroscience 2020Quote: ... 3’-Diaminobenzidine (Invitrogen, 750118) as a substrate ...
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bioRxiv - Neuroscience 2020Quote: ... 3 mM DTT (Invitrogen) and 40 units RNAse OUT (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μM MMC (ThermoFisher) for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: A 3% agarose (Invitrogen) gel solution was prepared in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... IL-3 (Life Technologies), Dexamethasone (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... QuantStudio 3 qPCR (Thermofisher) with KAPA Library Quantification Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO-2/3 (Invitrogen); β-Catenin (BD Transduction Laboratories) ...
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bioRxiv - Immunology 2022Quote: ... and 3% FBS (Invitrogen). Epidermal sheets were separated from the dermis after incubation for 45 min at 37°C in 2.4 mg/ml of Dispase and 3% FBS and the epidermis was further digested for 30 min in PBS containing 1 mg/ml collagenase D ...
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bioRxiv - Neuroscience 2022Quote: ... QuantStudio 3 from ThermoFisher was used ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... on QuantStudio 3 (ThermoFisher) and data were quantified by the 2-ΔΔCT method.
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bioRxiv - Biophysics 2023Quote: ... DiIC18(3) stain (Invitrogen). Transferrin from Human Serum ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 mL Trizol (ThermoFisher) was added to 1 mL of cellular PBS suspension in a 15 mL test tube ...
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bioRxiv - Biophysics 2023Quote: ... 3 mM DDT (Invitrogen), 1.5 µM of primers listed in Supplemental Table S6 ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Invitrogen, 16140071), 1% GlutaMAX ...
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bioRxiv - Genetics 2024Quote: ... 3% ES-FBS (Gibco), 0.1 mM β-Mercaptoethanol (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... Qubit 3 (Fisher Scientific) and 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... mixed with forward and reverse detection primers (T3DCD S1 forward [5’- TACGCGTTGATCACGACAAT-3’] and T3DCD S1 reverse [5’- TGGCGAGATTATTCCCTGAC-3’] or GAPDH forward [5’- ACCCAGAAGACTGTGGATGG-3’] and GAPDH reverse [5’- GGATGCAGGGATGATGTTCT-3’]) and SYBR Select Master Mix (Applied Biosystems), and then subjected to PCR using the StepOnePlus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...