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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Enzymatic activity was measured via the derived products generated from a 4-MUG substrate with a Fluoroskan Ascent II (Thermo Fisher Scientific). GUS activity is presented in an arbitrary unit as the ratio between fluorescence data par minute and protein concentration.
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bioRxiv - Cell Biology 2024Quote: ... Cell viability and metabolic activity of both HPSC and THP-1 cells were assessed by adding Resazurin (1µg/mL, Thermo Fisher Scientific Inc.) to treatments in 96-well plates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Selectivity studies were contracted to Thermo Fisher to run their panel screen of 408 protein kinases which can be found at https://www.thermofisher.com/uk/en/home/industrial/pharma-biopharma/drug-discovery-development/target-and-lead-identification-and-validation/kinasebiology/kinase-activity-assays.html against 1 μM of BI9774 (Thermo Fisher Scientific, UK); and in dose response against a panel contracted out to Eurofins consisting of 70 other custom selected diverse targets from their catalogue https://www.eurofinsdiscoveryservices.com/services/adme-tox/in-vitro-toxicology/ (Eurofins Cerep ...
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bioRxiv - Developmental Biology 2024Quote: For measuring in-gel activity the protocol was slightly modified by using 200 ml of Native PAGE anode buffer (Thermo Fisher Scientific) supplemented with 0.022 g Coomassie Brilliant Blue G-250 instead of the regular light blue cathode buffer.
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bioRxiv - Neuroscience 2022Quote: Primary spinal microglia grown on poly D-lysine coated glass-bottom dishes were loaded with Fura-2 by incubating cells in 2 uM Fura-2-AM (Invitrogen) in growth medium for 30 min at 37℃ ...
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bioRxiv - Immunology 2023Quote: ... 1-2 x 106 antigen-experienced 5c.c7 TCR-transgenic T-cells were incubated with 2 µM Fura-2-AM (Life technologies) in T-cell growth medium for 15-20 minutes at room temperature and subsequently washed with warm (room temperature ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of Superscript IV enzyme, 2 µL of 100 mM DTT and 2 µL of RNAseOut rnase inhibitor, Invitrogen) were added to each well ...
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bioRxiv - Bioengineering 2022Quote: ... formalin-fixed fibrotic capsule tissue samples (n=2 per group) and subcutaneous tissue samples (n=2) were placed in a solution of 2 mg/ml FITC (Invitrogen) in DMEM/F12 media (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME) (All from Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Nuclear factor erythroid 2-related factor 2 (NRF2) (ThermoFisher Scientific, Carlsbad, CA), samples diluted 1:2 ...
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bioRxiv - Immunology 2021Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020) ...
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bioRxiv - Microbiology 2020Quote: ... and 20 U/ml recombinant interleukin-2 (IL-2; Thermo Fisher Scientific) at 37° C ...
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bioRxiv - Neuroscience 2020Quote: ... 50mg/ml streptomycin and 2 mM GlutaMax supplemented with 2% B27 (Gibco) and 5% horse serum (Hyclone) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 2 μL forward primer and 2 μL reverse primer (Invitrogen; Table 1), 14.2 μL water (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cardiomyocytes were loaded with Fura-2 acetoxymethyl ester (Fura-2 AM; Invitrogen) as described previously.28 After Fura-2 loading ...
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bioRxiv - Immunology 2022Quote: ... + 200 IU/mL IL-2 (NCI) + 0.08 mM 2-Mercaptoethanol (Gibco, 21985023) (NK-92 Media ...
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bioRxiv - Immunology 2022Quote: ... 20 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... 2 mM L-glutamine and 53 μM 2-mercaptoethanol (all from Gibco). B cells were isolated using EasySep human naïve B cell Enrichment Kit (Stemcell Cat.# 19254 ...
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bioRxiv - Immunology 2019Quote: ... 2 mM L-glutamine and 53 μM 2-mercaptoethanol (all from Gibco) and activated with 2 μg/mL anti-mouse RP105 clone RP/14 (produced in house or BD Pharmingen Cat.# 562191).
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 2 μM fura-2 AM (Invitrogen, Waltham, MA) for 25 minutes at 35°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2 μl of 2 × Superscript III enzyme blend (Life Technologies, 11754). All the reactions were incubated at 25 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 2 min incubation in 2-propanol (Thermo Fisher Scientific, USA) and equilibration with 25 µl of solvent A (0.1% FA in H2O ...
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bioRxiv - Immunology 2024Quote: ... or anti-MD-2 (2 µg/ml) (Thermo Fisher Scientific, PA5-20058) for 3 h at RT ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell extracts obtained from 2×105 cells with an NE-PER Nuclear and Cytoplasmic Extraction kit (Thermo Fisher Scientific, 78833) were loaded into a 12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...
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bioRxiv - Immunology 2021Quote: Cells exposed to SARS-CoV-2 pseudovirus were lysed and mRNA was isolated with the mRNA Catcher™ PLUS Purification Kit (ThermoFisher). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... rRNA was removed from 20 μg of RNA with 2 reactions of the MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg linearized plasmid DNA was used in a 30 µl reaction with the mMessage mMachine SP6 Transcription Kit (Invitrogen: AM1340). RNA integrity was examined on a 1% agarose gel ...
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bioRxiv - Molecular Biology 2020Quote: ... was performed as follows: 2 μg of messenger RNA (mRNA) was reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Immunology 2022Quote: ... the SARS-CoV-2-TCR mRNA was transcribed in vitro using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Scientitic) following the manufacturer’s protocols ...
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Developmental Biology 2019Quote: PAT-PCR for the FBF target cyb-2.1 and control tbb-2 was performed using a Poly(A) Tail-Length Assay Kit (Thermo Fisher Scientific). RNA templates from fbf-1(lf) ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the Express one step RT-qPCR Universal kit (ThermoFisher Scientific) using 3.5µL of RNA and 6.5µL of RT-qPCR mix that contains 250nmol of each primer and 75nmol of probe ...
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bioRxiv - Neuroscience 2019Quote: The study of proliferation and differentiation of neural stem cells in the dentate gyrus was performed by incorporation of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Imaging Kit; Molecular Probes) to allow the analysis of proliferation and differentiation ...
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bioRxiv - Molecular Biology 2019Quote: ... LB was added to the sorted cells and they were grown at 37°C for 2 hours prior to plasmid isolation using PureLink HiPure Miniprep Kit (Thermo Fisher).
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bioRxiv - Cell Biology 2019Quote: ... Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific). 25 μL of 5 pM library DNA (1.25*10−4 pmol ...
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bioRxiv - Genomics 2019Quote: ... Extracted RNA (500 ng to 2 µg) were used as templates to make cDNA libraries using a High Capacity RNA-to-cDNA kit (Applied Biosystems). TaqMan gene expression assays were designed using GAPDH (Hs03929097 ...
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bioRxiv - Immunology 2021Quote: ... typically 50-100 pmol sgRNA was delivered into 2*105 cells per electroporation using the Neon 10 µL Kit (Invitrogen, #MPK1096) or 500-1000 pmol of sgRNA delivered into 2*106 cells per electroporation using the Neon 100 µL Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-2 μg of total RNA was used to generate cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Gene expression in mouse cells was quantified using specific primers from Life technologies for mGAPDH (Mm99999915_g1) ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA (2 μg) was used as a template for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). For qRT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... 4 µl of 5X first-strand reverse transcriptase (RT) buffer mixed with 2 µl DTT and 0.2 µl SuperScript™ III Reverse Transcriptase kit (Invitrogen, USA) (200U/µL ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA synthesis and qPCR were performed with the DyNAmo SYBR Green 2-Step qRT-PCR Kit (Thermo Scientific, Waltham, USA).
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bioRxiv - Evolutionary Biology 2021Quote: ... The final pooled library was diluted to an appropriate concentration and then subjected to Ion Sphere Particle (ISP) emulsion PCR amplification using an Ion One Touch 2 and an Ion PI Template OT2 200 Kit (Life Technologies). We sequenced the final library in two runs on an Ion Torrent Personal Genome Machine (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 2005) Reverse transcription (RT) of total RNA (2 µg) was carried out using the High-Capacity cDNA Achieve kit (Applied Biosystems) following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...