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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 2 min incubation in 2-propanol (Thermo Fisher Scientific, USA) and equilibration with 25 µl of solvent A (0.1% FA in H2O ...
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bioRxiv - Immunology 2024Quote: ... or anti-MD-2 (2 µg/ml) (Thermo Fisher Scientific, PA5-20058) for 3 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The fluorgenic substrate (5 μM) was mixed with control and test samples in 96 well plates (Black wall plate, Nunc), incubated for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were heat shocked in 50 μL (OD600 = 0.04) and transferred to plates (Nunc 96-Well Optical-Bottom Plate) containing 150 μL of their respective medium (final of OD600 = 0.01) ...
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bioRxiv - Microbiology 2020Quote: ... 10μl of lysate from the cell culture plate mixed then was transferred to a white Nunc 96-microwell plate (ThermoFisher) and 100μl of luciferase assay reagent (Promega Kit ...
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bioRxiv - Biochemistry 2022Quote: ... GTPase assays were performed in 200µl volumes in 96-well plates and the release of inorganic phosphate (Pi) was monitored at 360nm using the MultiSkan Go plate reader (ThermoFisher). Assays were performed in triplicates and a reference data set containing reaction buffer substrate (MESG ...
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bioRxiv - Immunology 2022Quote: ... the plates were read at multiple wavelengths (450 nm and 550 nm) on a plate reader (Multiskan GO, Thermo Fisher). The raw data were processed by Prism 9 (GraphPad ...
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bioRxiv - Bioengineering 2019Quote: ... the cells sampled at the same time points and diluted in PBS had their OD and YFP fluorescence (488nm excitation and 527nm emission) measured on a SynergyMX microtiter plate reader using clear-bottomed black microtiter plates (Thermofisher), and fluorescence values normalized to sample OD following the subtraction of background fluorescence from CEN.PK 113-7D ...
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bioRxiv - Synthetic Biology 2020Quote: Transformants were selected by plating first on YPD plates and then replica plating to yeast agar plates including 600 mg/L geneticin (Gibco) and incubating for a further 16 hours.
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bioRxiv - Biochemistry 2021Quote: 24.5 μL of 5 μM solution of every protein was prepared in 96-well plate MicroAmp Optical plate (Applied biosystems), and incubated at room temp ...
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bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
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bioRxiv - Developmental Biology 2022Quote: ... OPCs were seeded at 40,000 cells per well in 96-well plates or 50 million cells per 500 cm2 bioassay plate (166508, Thermo Fisher) (for generating large quantities of cells for sequencing studies ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL RNA + 95 μL H2O) into a new RNA dilution plate (96-well PCR quality plate – Thermo Scientific #AB0900). The final in-well concentration of the diluted RNA was in the range of 0.2 to 5 ng/μL.
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bioRxiv - Neuroscience 2022Quote: ... col seeded NGM plates and then moved to OP50 seeded plates containing 10 µM ThermoFisher scientific LysoTracker Blue DND-22 (Invitrogen) (1mM stock diluted 1:100 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 µL of culture with 26’000 cells was transferred in each well of the plate Nunc Edge 96-well plate (Thermo Scientific). The transfection mixture was added to the cells once it has incubated for approximately 30 min.
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Two microliters of each CFPS reaction were diluted with 33 μL of phosphate-buffered saline and analyzed in triplicate on a black 384-well plate with a multimode plate reader Varioskan Lux (ThermoFisher) at 488 nm excitation and 535 nm emission.
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bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
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bioRxiv - Cell Biology 2023Quote: ... In a next step 25µl of the compound cell culture media mix were carefully transferred from the intermediate plate on plates with the cells using a Beckman Coulter Biomek Fx followed by incubation in the Cytomat incubator (ThermoFisher) at 37°C and 5% CO2 for 24 and or 72 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fifty hydras were individually maintained at 18°C in cell culture plates (12-well plates, 1.5 mL/well, Thermo Scientific) filled with Volvic© water under a photoperiod of 12h ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells were seeded at a density of 15,000 cell/cm2 in either 24-well plates or 12-well plates on #1.5 glass coverslips (Thermo Fisher Scientific). The following day ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell extracts obtained from 2×105 cells with an NE-PER Nuclear and Cytoplasmic Extraction kit (Thermo Fisher Scientific, 78833) were loaded into a 12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...
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bioRxiv - Immunology 2021Quote: Cells exposed to SARS-CoV-2 pseudovirus were lysed and mRNA was isolated with the mRNA Catcher™ PLUS Purification Kit (ThermoFisher). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... rRNA was removed from 20 μg of RNA with 2 reactions of the MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg linearized plasmid DNA was used in a 30 µl reaction with the mMessage mMachine SP6 Transcription Kit (Invitrogen: AM1340). RNA integrity was examined on a 1% agarose gel ...
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bioRxiv - Molecular Biology 2020Quote: ... was performed as follows: 2 μg of messenger RNA (mRNA) was reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Immunology 2022Quote: ... the SARS-CoV-2-TCR mRNA was transcribed in vitro using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Scientitic) following the manufacturer’s protocols ...
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Developmental Biology 2019Quote: PAT-PCR for the FBF target cyb-2.1 and control tbb-2 was performed using a Poly(A) Tail-Length Assay Kit (Thermo Fisher Scientific). RNA templates from fbf-1(lf) ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the Express one step RT-qPCR Universal kit (ThermoFisher Scientific) using 3.5µL of RNA and 6.5µL of RT-qPCR mix that contains 250nmol of each primer and 75nmol of probe ...
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bioRxiv - Neuroscience 2019Quote: The study of proliferation and differentiation of neural stem cells in the dentate gyrus was performed by incorporation of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Imaging Kit; Molecular Probes) to allow the analysis of proliferation and differentiation ...
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bioRxiv - Molecular Biology 2019Quote: ... LB was added to the sorted cells and they were grown at 37°C for 2 hours prior to plasmid isolation using PureLink HiPure Miniprep Kit (Thermo Fisher).
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bioRxiv - Cell Biology 2019Quote: ... Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific). 25 μL of 5 pM library DNA (1.25*10−4 pmol ...
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bioRxiv - Genomics 2019Quote: ... Extracted RNA (500 ng to 2 µg) were used as templates to make cDNA libraries using a High Capacity RNA-to-cDNA kit (Applied Biosystems). TaqMan gene expression assays were designed using GAPDH (Hs03929097 ...
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bioRxiv - Immunology 2021Quote: ... typically 50-100 pmol sgRNA was delivered into 2*105 cells per electroporation using the Neon 10 µL Kit (Invitrogen, #MPK1096) or 500-1000 pmol of sgRNA delivered into 2*106 cells per electroporation using the Neon 100 µL Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-2 μg of total RNA was used to generate cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Gene expression in mouse cells was quantified using specific primers from Life technologies for mGAPDH (Mm99999915_g1) ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA (2 μg) was used as a template for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). For qRT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... 4 µl of 5X first-strand reverse transcriptase (RT) buffer mixed with 2 µl DTT and 0.2 µl SuperScript™ III Reverse Transcriptase kit (Invitrogen, USA) (200U/µL ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA synthesis and qPCR were performed with the DyNAmo SYBR Green 2-Step qRT-PCR Kit (Thermo Scientific, Waltham, USA).
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bioRxiv - Evolutionary Biology 2021Quote: ... The final pooled library was diluted to an appropriate concentration and then subjected to Ion Sphere Particle (ISP) emulsion PCR amplification using an Ion One Touch 2 and an Ion PI Template OT2 200 Kit (Life Technologies). We sequenced the final library in two runs on an Ion Torrent Personal Genome Machine (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 2005) Reverse transcription (RT) of total RNA (2 µg) was carried out using the High-Capacity cDNA Achieve kit (Applied Biosystems) following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...