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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Cardiomyocytes were loaded with 2 μM Fura-2 (Thermo Fisher, F1201) in Tyrode’s solution for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... and 1 ug/ml of interleukin-2 (IL-2; Gibco BRL) in complete RPMI 1640 containing 2mM L-glutamine ...
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 2 μM fura-2 AM (ThermoFisher Scientific) in BSS for one hour at 37 °C and rinsed twice with BSS (Gibson et al ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM 3-methyl-2-oxobutanoic acid (Fisher Scientific, Hampton, NH) and 1 mM acetyl-CoA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... in a 2:2:1 molar ratio using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (Thermo Fisher), and 1X non-essential amino acids (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: Fura-2 pentaacetoxymethyl ester (fura-2/AM) was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2024Quote: ... 1% N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco, #15630080) and 1% MEM nonessential amino acids (MEM-NEAA ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 mM GlutaMax supplemented with 2% (wt/vol) B27 (GIBCO) and 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... in a 2:2:1 molar ratio using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µg of total RNA were reverse transcribed with High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... Approximately 2 μg of RNA from each sample were DNAse treated with the DNA-free™ DNA Removal Kit (Invitrogen) in the presence of RiboLock RNase Inhibitor (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Cell Biology 2022Quote: ... First strand cDNA was synthesized using 2 μg of total RNA using Maxima H Minus Kit according to manufacturer instructions (ThermoFisher). qRT-PCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: Enzyme-linked immunosorbent assay (ELISA) was performed for IFNγ and IL-2 using uncoated kits (respectively 88-7316-88 and 88-7025-88, ThermoFisher) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... PA) and cDNA was synthesized from 2 μg RNA using a High Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... transfected DC2.4 cells were cocultured for 5 days with 2 * 106 Thy1.1+/1.1+ P14 cells purified as detailed above and stained with CellTrace Violet Proliferation kit (Invitrogen, cat. C34557) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 2 µl PCR reaction were directly taken for TOPO cloning with Zero Blunt™ TOPO™ PCR Cloning Kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Emulsion PCR was performed using the Ion PGM Hi-Q View OT2 Kit and the Ion OneTouch 2 system (ThermoFisher). Template-positive ion sphere particles (ISPs ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: The supernatants were collected after cytotoxicity assay for cytokine analysis by using IFN-γ and IL-2 ELISA kits (Invitrogen). Cytokine levels were analyzed by comparing the values obtained from samples to a standard curve generated from known concentrations of the cytokines.
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... extracted DMS-modified RNA from SARS-CoV-2 infected Vero cells was fragmented using the RNA Fragmentation kit (ThermoFisher Scientific). 1.5 μg of rRNA subtracted total RNA was fragmented at 70°C for 2.5 min ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was prepared using 2 µg of total RNA with a high-capacity cDNA Reverse Transcription Kit (Applied Biosystems). All candidate gene primers were designed using a unique sequence within the genes preferably 3’UTR ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of DNAse-treated RNA was used for cDNA synthesis using the RevertAid cDNA Synthesis Kit (Thermo Fisher Scientific). The gene expression analysis for Prnp and Gapdh was performed using the respective TaqMan probes (Thermo Scientific Fisher) ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 detection was performed by quantitative real time PCR using Taqpath COVID-19 combo kit (Applied Biosystems, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen and designed for the human sequences of cholesterol biosynthesis pathway genes ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 ug of total RNAs were then used for cDNA synthesis using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative RT-PCR was performed ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Cancer Biology 2020Quote: ... The extracted RNA was reverse-transcribed into cDNA using the Superscript VILO cDNA synthesis kit (Thermo Fisher Scientific, Figure 2) or SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA ...
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bioRxiv - Cell Biology 2020Quote: ... The marcksl1a and marcksl1b DIG-labeled 1.2 kb long riboprobes were generated from plasmids encoding the full-length cDNAs using MEGAscript SP6 or T7 kits (Invitrogen). Embryos were cleared in 70% glycerol overnight and imaged using Leica M205FA microscope.
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bioRxiv - Cell Biology 2020Quote: ... 5-ethynyl-2-deoxyuridine (EdU) labelling performed with Invitrogen Click-iT EdU Alexa Fluor 594 Imaging Kit (Life Technologies, C10339).
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bioRxiv - Microbiology 2022Quote: ... 0.5 to 2 g of RNA was reverse transcribed with the high-capacity cDNA reverse transcription (RT) kit (Applied Biosystems) using gene-specific primers ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 E sgmRNA standards were generated by in-vitro transcription of the generated SARS-CoV-2 E sgmRNA cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA mini kit (Invitrogen) and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... we performed 5-ethynyl-2′-deoxyuridine (EdU) staining using the Click-iT EdU Alexa Fluor 647 imaging kit (C10340, Invitrogen). Four-color image acquisition of three replicates per condition was performed on an SP8 confocal microscope (Leica) ...
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bioRxiv - Cell Biology 2022Quote: Surface proteins on Caco-2 cells were biotinylated using Pierce Cell Surface protein biotinylation and Isolation Kit (Thermo Scientific, A44390) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 2 μg total RNA in 20 μL reaction mixture using a cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized from 2 µg RNA using the Maxima First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, Massachusetts, USA). All qPCR analyses were performed in technical replicates ...
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bioRxiv - Cell Biology 2021Quote: NRVMs were derived from 1- or 2-day old Sprague-Dawley rates using the Cellutron Neomyt kit (Thermo Fisher Scientific). Cell purity was routinely >90% cardiomyocytes ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen with PPIA ...
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bioRxiv - Molecular Biology 2019Quote: cDNA was generated from 0.5-2 μg of RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) in 20 μL final volume following manufacturer’s instructions ...