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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 25 μL of media containing STS and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at final treatment concentrations of 1 μM and 2.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... The electrodes were labelled with DiIC18(7) (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine Iodide) (DiR) (Invitrogen) to enable post-mortem reconstruction of the electrode tracks in histological sections.
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 488 and caspase-3/7 activity detection dyes were purchased from Thermo Fisher, and Viobility™ 405/452 Fixable Dye from Miltenyi Biotec.
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bioRxiv - Microbiology 2023Quote: ... Apoptosis assays were perfomed using CellEvent Caspase 3/7 Green Detection Reagent (Thermo Fisher Scientific) and Live/Dead eFluor 660 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... lung tissues were incubated with primary antibodies diluted in PBS with 0.3% TritonX-100 on a rocker at 4° C overnight. Primary antibodies used (Fig. 5) were: rat anti-E-cadherin (E-Cad, 131900, Invitrogen; 1:1000), goat anti-CCSP (1:2,500 ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was refreshed every day and cells were passaged every 4-5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 5% (w/v) skimmed milk overnight at 4°C and probed with anti-DsbA (1:1000, Thermo Fisher Scientific) or anti-AP (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were collected by spinning at 1000g for 5 min at 4°C and cell pellets were resuspended in 1 mL of Trizol (Thermo Fisher Scientific). Total RNA was extracted according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). SARS-CoV-2 standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g.
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Gibco). Inpp4b+/+ and Inpp4b-/- LSK were each retrovirally transduced with pMSCV-MLL-AF9-IRES-mVenus ...
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bioRxiv - Microbiology 2023Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). SARS-CoV-2 standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g ...
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bioRxiv - Genomics 2021Quote: ... were grown in DMEM medium (4.5 g/l glucose, 6 mM L-glutamine, Gibco), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... 600 ng VSV-G envelope plasmid and 6 μL of Lipofectamine 2000 (Thermo Scientific). Supernatants were collected at 48 and 72 h post-transfection and clarified using 0.45 μm filters ...
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bioRxiv - Genomics 2021Quote: ... 50μg isotope-matched IgG and 50μl protein A/G agarose beads (A:G = 1:1, ThermoFisher 15918014 and ThermoFisher 15920010) and rotated at 4℃ for 8h to preclear the chromatin ...
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bioRxiv - Genomics 2020Quote: ... After 1 hour incubation in 300 μl of DPBS/1%FBS /DAPI 2.5 g/ml (Thermo Fisher Scientific D1306), the samples were analyzed using an LSR II FACS (BD) ...
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bioRxiv - Cancer Biology 2022Quote: ... Spin infections were performed at room temperature at 1,500 × g for 1 hour with polybrene reagent (1:2,000; Fisher Scientific). For the generation of hBMSC-CM ...
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bioRxiv - Cancer Biology 2019Quote: ... The 1% ITS medium contained the RPMI-1640 medium with 1 × Insulin-Transferrin-Selenium (ITS-G, Thermo Fisher Scientific), 2 mM L-glutamine (Gibco ...
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bioRxiv - Genomics 2020Quote: ... The DNA was captured on a 1:1 mixture of protein A and G Dynabeads (Invitrogen 10001D and 10003D). After washes ...
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bioRxiv - Genomics 2022Quote: ... Indicated antibodies (anti-Flag, 1:2000, 12793S, Cell Signaling Technology; anti-Rabbit-IgG, 1:2000, G-21234, Thermo Scientific; anti-β-actin ...
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bioRxiv - Plant Biology 2022Quote: ... and G when used at 1:2,000 dilution) and anti-GFP conjugated with horseradish peroxidase (Invitrogen, 1:2,000 dilution) with gentle shaking overnight at 4°C to detect MMV proteins and GFP respectively ...
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bioRxiv - Microbiology 2023Quote: ... 0.71 g × L−1 of Na2HPO4, 0.58 g × L−1 of NaCl in deionized water, followed by autoclave, pH 7.4; Acros organics) three times using 100 kDa amicon filters (Merck ...
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bioRxiv - Systems Biology 2023Quote: ... Remaining insoluble debris were removed by centrifugation for 1 min at 1,500 g before addition of 1 U of RNase I (Ambion) per µg RNA and 0.134 U TURBO DNase (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... on ice and dilute 1:200 in cold DMEM high glucose (4500 g dl-1 glucose; Thermo Fisher - 41966029)
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bioRxiv - Genomics 2020Quote: ... Matching regions from 3 adjacent sections were collected on one Capsure Macro Cap (Thermofisher), region size permitting ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were stained for one hour before induction using SP- DiIC18(3) (Invitrogen, #D7777) to fluoresce at 564nm or DiIC18(5 ...
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bioRxiv - Microbiology 2021Quote: ... were mixed with 1 pmol of AS primer 1 (5’-TGGGTGGTACTTAAGTTCGG-3’: complementary to nts 476-495 of A3G RNA) labeled with Vic (Life Technologies SAS, France). The mixture was heated at 90°C for 2 min and placed on ice for 2 min ...
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Dishes were washed with PBS 5 times for 3 min each before applying donkey anti-mouse Alexa Fluor 568 secondary antibodies (A10037, Invitrogen; 1:2000 diluted) and Alexa Fluor 647 phalloidin (A22287 ...
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bioRxiv - Cell Biology 2024Quote: ... 2.0µM Template Switching Oligo (TSO) (5′-Biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT) and 2U µl−1 of Maxima H Minus reverse transcriptase (Thermo Fisher Scientific, #EP0752). Plates were quickly centrifuged before incubation at 42°C for 90min ...
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bioRxiv - Biochemistry 2024Quote: ... to visualize CaSR-containing cells and for 5 minutes with 1:1000 TO-PRO-3 iodide (#T3605, Thermo Fisher Scientific, Waltham, MA, USA) to visualize permeable cells ...
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bioRxiv - Immunology 2023Quote: ... 7-Aminoactinomycin D (7-AAD) (Invitrogen) at 1:400 was used as a marker for dead cells ...
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bioRxiv - Pathology 2023Quote: ... one lung section 5×5×5 mm in size was finely cut and stored at -20°C in RNAlater (Thermo Scientific, US). We mechanically disrupted the tissue from RNAlater using a MediMachine (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... detection cells were incubated for 6h and stained using Click-iTTM EdU Alexa FlourTM 594 HCS Assay (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... followed by 4 hours with Dynabeads™ Protein G (Thermo Scientific) at 4°C ...
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bioRxiv - Microbiology 2023Quote: 3’(4-Hydroxyphenyl)-fluorescein (HPF; Molecular Probes, OR, USA) was used for detecting •OH production (Avci et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM EDTA (pH 8) (TE) containing 10 mg/mL RNase A (Thermo Scientific), and incubated at 37 °C for 1 hr ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were permeabilized for 8 minutes at -20ºC in Optima Methanol (ThermoFisher, A456-1)and washed in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... then transferred into a 1:8 dilution of 10 kD fluorescein conjugated dextran (Invitrogen) in Schneider’s Insect Medium (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... followed by immediate neutralization with 1 M Tris-HCl pH 8 (Thermo Fisher Scientific). Following elution from the column ...
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bioRxiv - Plant Biology 2024Quote: ... 5 mM EDTA, 5 mM DTT, 10 mM β-mercaptoethanol, 1% SDS, 1 mM PMSF, and 1X protease inhibitors from Thermo Fisher), and the plant debris was removed by centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated with fluorescently labelled secondary antibodies (1:1,000 G-anti-Rb-AlexaFluor-488, G-anti-M-AlexaFluor-555, ThermoFisher Scientific) and DAPI in PBS+ for 1.5 hr at room temperature before mounting with Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Cell Biology 2023Quote: ... coated #1.5 18 mm glass coverslips in DMEM (4.5 g/L-1 D-G-glucose) supplemented with 10% fetal bovine solution (Gibco™), 1% antibiotic solution (penicillin Streptomycin ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... After neutralization with 1/10 volume of 1 M sodium phosphate buffer (pH 8) the antibody was coupled to M270 Epoxy Dynabeads (Thermo Scientific) in ammonium sulfate buffer (0.1 M sodium phosphate pH 7.4 ...