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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 Boc 1 oxa 6 azaspiro 3.3 heptan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HSC (Lin−Sca-1+c-Kit+CD150+CD48−) from wildtype C57BL/6 mice were sorted into 16-well chamber slides (Thermo Fisher Scientific) coated with RetroNectin (Takara Bio) ...
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bioRxiv - Bioengineering 2022Quote: ... D13+14 PT-enhanced organoids (triplicate wells per condition) were incubated in 4’,6-diamindino-2-phenylindole substrate (DAPI; 1:1000 [Thermo Fisher Scientific]) with 1:500 DRAQ7 dead cell label (Thermo Fisher Scientific] ...
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bioRxiv - Neuroscience 2022Quote: ... prior to washing (6×60 min PBST) and secondary incubation with species specific Alexafluor IgG secondary antibodies (60 min RT, Invitrogen; 1:500). Tissues were washed again in 0.1% PBST (6 × 60 min) ...
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bioRxiv - Developmental Biology 2022Quote: Mice were anesthetized using isoflurane and injected with 1% Biocytin-5(and-6-)- Tetramethylrhodamine (Biocytin-TMR: ThermoFisher Scientific; Waltham, MA, Cat# T12921). After 30 minutes in circulation ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Virus mutant libraries were rescued in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence in a T75 flask (75 cm2) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: sg-mEX3 or sg-mEX5 and SpCas9-2A-GFP plasmids at 1:1 molar ratio were co-transfected into ~80% confluence OC-k1 cells in 6-well plate using Lipofectamine 3000 (Thermo Fisher Scientific). The cells were incubated for 48 hours and then submitted for fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected after 6 days and passed through a gravity chromatography column containing 1 ml of PierceTM Protein G Agarose resin (ThermoFisher Scientific 20397). The column was washed with 20 ml of wash buffer (150mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... 4*105 TX1072 cells cultured in SL medium were reverse transfected with 250 ng pX330-PK45/46 and 750 ng of the pA cassette-containing donor plasmid (SP292) with Lipofectamine 3000 reagent using 1 µl P3000 reagent and 6 µl Lipofectamine 3000 (Thermo Fisher Scientific) following the manufacturer’s guidelines ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... formation was induced by culturing cell aggregates in suspension from 1-well of a 6-well plate on a 35×10mm petri dish (Thermo Fisher Scientific) with mTeSR1 medium ...
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bioRxiv - Genetics 2020Quote: ... Confocal microscopy was carried out embedding the 6 dpf larvae in 1 % (w/v in E2 medium containing tricaine) low-melting agarose (Fisher Scientific, USA) in a 50-mm glass bottom dish (MatTek ...
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bioRxiv - Genetics 2022Quote: ... These pieces were transferred to the lower surface of a 25 cm2 culture flask (6-8 pieces per flask) which had been pre-moistened with 1-2 mL of AmnioMAX Complete Medium (Thermo Scientific, #11269016) supplemented with 1% penicillin/streptomycin (GenDepot ...
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bioRxiv - Biophysics 2022Quote: ... 1 x 106 Sf9 cells were transferred to each well of a six-wells plate and subsequently transfected with 1 μg of bacmid DNA by 6 μl of Cell-fectin (Thermo Fisher Scientific). Baculovirus containing cell supernatants (P1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Immunology 2022Quote: ... Media was removed from THP-1 monocytes cultures (5-6 x 105 cells/ml) and cells were washed once with DPBS (Thermo Fisher Scientifics). A total of 2 x 106 cells were resuspended in 50 μl of BTXpress solution and the Cas9-sgRNA complex was added ...
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bioRxiv - Microbiology 2023Quote: ... or macroH2A-3Xflag (6 x 106 on 100 mm dish) were cross-linked with 1 % of methanol-free formaldehyde (Thermo Scientific, 28906) in DMEM for 10 min at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... PCs were seeded into 6-well plates coated with collagen IV (100 μg/mL) and were cultured in DMEM (1 g/L glucose, Gibco, cat# 11885084) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with serum-free RPMI-1640 medium before being transfected with 5 µL of siRNA pool (20 µM) and 5 µL Lipofectamine 2000 in a 6-well plate in 1 mL of antibiotic-free Opti-MEM (Gibco, #31985-062) medium plus 2 mL antibiotic-free cell-culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 1 µg of the indicated plasmids (6-well plates) by using the Lipofectamine 2000 system (Thermo Fisher Scientific). For transient transfection with siRNA ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2023Quote: ... The pellets were resuspended in 4.5 mL distilled water to which is added 0.5 mL 6% hypochlorite solution (Fisher Scientific Cat# SS290-1) and gently rocked for 1 minute followed by centrifugation (800g for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 6×10 minutes in .05M PBS and incubated with both Alexa-Fluor 488 and 555 secondary antibody (1:1000; Thermo Fisher Scientific) in .05M PBS with .1% Triton x100 for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... was used to amplify the genes of interest according to the primers mentioned in the Table 1 from 15-20 ng of cDNA in the QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific) machine ...
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bioRxiv - Microbiology 2023Quote: ... 10 x 1 µL droplets of each diluted suspension were deposited on polystyrene material (6-well plates, Thermo Scientific, Cat. No. 140675) and exposed to ambient temperature and variable RH (20% ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and placed in a 6-well cell culture plate filled with 1 ml/well of culture media containing 50% Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23% Earle’s Balanced Salt Solution ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cell Biology 2023Quote: ... 2 x 105 cells were seeded in a 6-well plate containing coverslips and transfected with 1 µg dsRNA mixed with Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio ...
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bioRxiv - Plant Biology 2024Quote: ... were used to amplify a 1:16 dilution of cDNA in triplicate in a Quantstudio™6 PRO real-time thermal cycler (ThermoFisher, Inc.) with a 61°C annealing temperature ...
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bioRxiv - Genetics 2024Quote: ... RT-PCR products were fractionated by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (ThermoFisher Scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ v1.47 (NIH ...
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bioRxiv - Immunology 2024Quote: MonoMac-6 effector cells were stained with 1 μM of CellTrace™ Far Red dye (CTFR, cat. no. C34564, Thermo Fisher Scientific) in PBS (cat ...
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bioRxiv - Neuroscience 2024Quote: ... into the medial septal area (MS) of C57Bl/6NTac mice (n=6). For the colocalization experiments (Fig. 1) we injected yellow-green FluoSpheres (Thermo Fisher Scientific) into the MS of two C57Bl/6NTac mice or AAVrg-syn-FLEX-jGCaMP8m-WPRE (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... 1.7 x 105 Huh7 cells were seeded in a 6-well plate and transfected for 16 h with 1 µg purified dsRNA mixed with Lipofectamin 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio prior to actinomycin D chase.
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 20 minutes with 3.3 nM phalloidin-AF647 (Thermo Fisher, A22287) in the (d)STORM imaging buffer (Abbelight ...
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bioRxiv - Cell Biology 2020Quote: ... Cryo-light and cryo-SEM images were correlated using the MAPS 3.3 software (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Nocodazole (NZ) – exponentially growing cells were treated with 3.3 µM nocodazole (Fisher Scientific Ltd 15997255) for 24 h to arrest them at G2/M ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Biophysics 2019Quote: ... N-((2-(iodoacetoxy)ethyl)-N-Methyl)- amino-7-Nitrobenz-2-Oxa-1,3-Diazole (IANBD ester) and Oregon Green 488 maleimide were purchased from LIFE TECHNOLOGIES LTD (Paisley ...
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bioRxiv - Biophysics 2019Quote: ... N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine] (Thermo Fisher Scientific, Inc., Waltham, MA). Y-ATRAM (Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...