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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 6 Boc 1 oxa 6 azaspiro 3.3 heptan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... or macroH2A-3Xflag (6 x 106 on 100 mm dish) were cross-linked with 1 % of methanol-free formaldehyde (Thermo Scientific, 28906) in DMEM for 10 min at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... PCs were seeded into 6-well plates coated with collagen IV (100 μg/mL) and were cultured in DMEM (1 g/L glucose, Gibco, cat# 11885084) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Biophysics 2019Quote: ... N-((2-(iodoacetoxy)ethyl)-N-Methyl)- amino-7-Nitrobenz-2-Oxa-1,3-Diazole (IANBD ester) and Oregon Green 488 maleimide were purchased from LIFE TECHNOLOGIES LTD (Paisley ...
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bioRxiv - Biophysics 2019Quote: ... N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine] (Thermo Fisher Scientific, Inc., Waltham, MA). Y-ATRAM (Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 20 minutes with 3.3 nM phalloidin-AF647 (Thermo Fisher, A22287) in the (d)STORM imaging buffer (Abbelight ...
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bioRxiv - Cell Biology 2020Quote: ... Cryo-light and cryo-SEM images were correlated using the MAPS 3.3 software (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Nocodazole (NZ) – exponentially growing cells were treated with 3.3 µM nocodazole (Fisher Scientific Ltd 15997255) for 24 h to arrest them at G2/M ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was conducted on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using 12 ng cDNA per reaction ...
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR was performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with LightCycler 480 SYBR Green I Master (Roche) ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Immunology 2021Quote: ... vRNA was quantified using the QuantStudio 6 Real-Time PCR System (Applied Biosystems, Waltham, MA). Five microliters vRNA was added in duplicate to a 0.1 mL 96-well MicroAmp fast optical reaction plate (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were seeded at equal densities into wells of 6-well plates (Nunc, Roskilde, Denmark). The cells were pre-treated with EVs for 24 h and then stimulated with LPS (LL24 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was quantified using Qubit high sensitivity assays (Thermo Fisher Scientific)6-10 μg of DNA was digested with 120 Units Csp6I enzyme (Thermo Fisher Scientific) [3-5 Csp6I digests per sample] overnight at 37°C in thermomixer shaking at 900 rpm (30s on ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were run on a QuantStudioTM 6 Flex Real-Time PCR System (ThermoFisher; Cat #: 4485691). Fold change calculations were done based on the ΔΔCt method ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.05 % xylene cyanol) and the products were separated on a 6 % urea-PAGE gels (Invitrogen) run at 180 V for 50 min ...
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bioRxiv - Neuroscience 2022Quote: ... We plated an amount of 6×105 primary mouse cortical neurons (ref. Gibco A15586, ThermoFisher) on each coated coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... We plated an amount of 6×105 primary mouse cortical neurons (ref. Gibco A15586, ThermoFisher) on each coated coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed in the QuantStudio 6 Flex Real-Time System (Applied Biosystems, Waltham, MA). Target gene expression levels were determined by the comparative 2∧-(delta C(T) ...
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bioRxiv - Neuroscience 2021Quote: ... and 5% (by volume) fluorescent bead solution (6 μm FocalCheck Microspheres; part no. F14807; ThermoFisher), prepared using deionized water ...
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bioRxiv - Neuroscience 2021Quote: ... fixed larvae were stained with 2.5 ug/ml 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS to label all cell nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed using the Quantstudio 6 Flex (Applied Biosystems, Life Technologies, USA). For the qPCR reaction 1 μl of 1:20 diluted cDNA in MilliQ was used with 1 μl primer mix (forward and reverse primers ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixtures were run on a QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with combined annealing and elongation cycles (Step1 ...
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bioRxiv - Molecular Biology 2019Quote: ... 6’-Diamidino-2-phenylindole (DAPI; Cat#D1306; RRID: AB_2629482) were purchased from Invitrogen (Carlsbad, CA). Neurobasal® medium ...
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bioRxiv - Immunology 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and LysoTracker Red DND-99 were purchased from Invitrogen, Carlsbad ...
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bioRxiv - Genomics 2020Quote: ... transduced cells were seeded into 6-well format in 1.5 μg/mL puromycin (Life Technologies) containing medium ...
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bioRxiv - Genomics 2020Quote: ... plated in 6-well plates then stimulated with anti-CD3/anti-CD28 Dynabeads (Life Technologies) over a period of 24-hours ...
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bioRxiv - Physiology 2019Quote: Total RNA was isolated from 6 adult female medaka (WT) pituitaries using Trizol (Ambion, USA) and cDNA was prepared from 30 ng total RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR assays were performed on either QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) or CFX384 Touch Real-Time PCR Detection System (BIO-RAD) ...
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bioRxiv - Microbiology 2019Quote: ... nuclei were stained using Prolong gold antifade containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). All preparations were observed with a confocal microscope (ZEISS LSM 700 inverted ...
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bioRxiv - Cancer Biology 2019Quote: ... then resuspended in the FACS buffer with 6 µg/mL Hoechst 33258 (Thermo Fisher; H3569) and either 2-µm YG microspheres (Polysciences ...
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bioRxiv - Synthetic Biology 2019Quote: Samples were resolved by electrophoresis in 6% or 15% polyacrylamide TBE-Urea gels (Thermo Fisher) in TBE ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... the cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, R37606). Cells were then viewed and photographed using a fluorescence microscope (Zeiss ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was then loaded on a Novex 6% TBE-Urea gel (Life Technologies) and a band around 160-170 bp was excised from the gel ...
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bioRxiv - Biochemistry 2020Quote: ... which were blotted for 4-6 sec using a Vitrobot Mk IV (Thermo Fisher Scientific) and vitrified by plunging into liquid ethane at liquid N2 temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then labeled using 6-plex tandem mass tags (TMT; Thermo Scientific, Waltham MA) according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Mounting media with DAPI (4’,6-diamidino-2-phenylindole) used was either ProLong Gold (Invitrogen) or ProLong Diamond (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... 6-well well-plates were first coated with vitronectin (5 μg/mL, Thermo Fisher, USA) followed by a coating with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... cell nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, dilactate; 2μM, Life Technologies). Cells were visualized in PBS with calcium and magnesium (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were stained with 4′-6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, CA, USA) before examination under a fluorescence microscope (Zeiss LSM 710).