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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 6 Boc 1 oxa 6 azaspiro 3.3 heptan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... hPSCs were cultured on Matrigel-coated 6-well plates in commercial StemFlex™ Medium (ThermoFisher). The medium was changed every day ...
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bioRxiv - Immunology 2020Quote: CRP and IL-6 were measured using a high sensitivity ELISA (Life Technologies, Carlsbad CA). Plasma soluble CD14 (sCD14 ...
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bioRxiv - Immunology 2020Quote: Cell culture supernatant (diluted ¼) was subjected to ELISA (TNFα, Thermo Scientific, Waltham, MA, n=6) and Luminex (Cytokine Human Magnetic 30 Plex Panel ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher) and 0.2% Penicillin/Streptomycin 7 days post transfection ...
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bioRxiv - Microbiology 2021Quote: ... and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC; Thermo Fisher Scientific), respectively ...
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bioRxiv - Immunology 2020Quote: ... calcein AM (C1430) and 4-sulfonic calix[6]arene (10494735) were purchased from Thermo Fisher. 4-[(2-Butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]butanoic acid (DCPIB ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins (approximately 6 μg/sample) were electrophoresed on 4-12% Bis-Tris polyacrylamide gels (Invitrogen) at 200V for 55 minutes ...
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bioRxiv - Genetics 2020Quote: ... EBs were plated on laminin (Cat# 23017015) coated 6-well plates (Nunc; Thermo Fisher Scientific) on day 7 ...
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bioRxiv - Genetics 2020Quote: ... EBs were plated on laminin (Cat# 23017015) coated 6-well plates (Nunc; Thermo Fisher Scientific) on day 7 ...
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bioRxiv - Genomics 2021Quote: ... In step 6 we used Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher Scientific) for 18 cycles of PCR ...
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bioRxiv - Cell Biology 2021Quote: ... 12-well or 6-well Nunclon™ Delta Surface multi well plates (Thermo Fisher Scientific). For live-cell imaging ...
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bioRxiv - Cell Biology 2021Quote: ... expanded in E8 on geltrex-coated 6 well plates and routinely passaged using Versene (Gibco). Pluripotency of the isolated iPSC lines was confirmed by immunofluorescence (IF) ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence signals were detected with a QuantStudio 6 Sequence Detector (Applied Biosystems, Foster City, CA). Data were captured and analyzed with Sequence Detector Software version 1.3 (Applied Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed using the Quantstudio 6 Flex (Applied Biosystems, Life Technologies, USA). For the qPCR reaction 1 μl of 1:20 diluted cDNA in MilliQ was used with 1 μl primer mix (forward and reverse primers ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... Slides were counterstained with 5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific) for 10 min at room temperature and washed with 1 × PBST (0.2% Triton-X 100 ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections (4-6 μm thickness) were mounted onto Superfrost® Plus microscope slides (Thermo scientific) or on Zeiss MembraneSlide 1.0 PEN NF (#415190-9081-001) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were stained with 20 μg/ml DAPI (4’,6-Diamidino-2-Phenylindole, Invitrogen) in PBS for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was loaded onto a Ni affinity column (Ni-Sepharose 6 FF, Invitrogen). The His-tag was cleaved by the HRV3C protease on the column ...
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was loaded onto a Ni affinity column (Ni-sepharose 6 FF, Invitrogen). GreA was eluted with the lysis buffer supplemented with 500 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... qPCR was performed on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) with steps of 10 min at 95 °C and 40 cycles of 15 s at 95 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... incubated in 6 ml of HBSS containing 0.15 mg/ml Collagenase I (Invitrogen, 17100-017) at 37°C for 35 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, in blue, Molecular Probes, Eugene, OR). The slides were observed with a confocal microscope (Fluoview FV100 ...
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bioRxiv - Cancer Biology 2022Quote: ... SlowFade™ gold antifade mountant containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) was applied to stain nuclei and reduce photobleaching ...
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bioRxiv - Immunology 2022Quote: We labeled MC38/gp100 and NALM-6 cells with Cell-Trace Violet (Invitrogen cat. # C34557) dye at a concentration of 5 μM ...
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bioRxiv - Immunology 2022Quote: Caco2 cells were grown in 6-wells and 12-wells cluster plates (Thermo Scientific Nunc Cell-Culture Treated ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each reaction consisted of 6 μl of PowerUp™ SYBR™ Green Master Mix (ThermoFisher), 0.5 μl of each primer solution at 3.6 μm and 5 μl of diluted DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 2 - 6 s in a Vitrobot (Mark IV, Thermo Fisher Scientific) at 4 °C and 100% humidity ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time PCR was performed with the QuantStudio 6 Flex (Applied Biosystems, Life Technologies) using SYBR qPCR master mix (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... each with an N-terminal (His)6 tag from plasmids generated by GeneArt (ThermoFisher Scientific). For each purification ...
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bioRxiv - Genomics 2022Quote: ... Real-time PCR was performed on a QuantStudio 6 Flex real-time thermocycler (Applied Biosystems) with the following thermocycling conditions ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected in 6 well plates at 60% confluence using lipofectamine LTX (Invitrogen) with 1µg of each transposon vector along with 0.5µg of pCMV-m7PB hyperactive transposase (34) ...
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bioRxiv - Molecular Biology 2022Quote: ... The transfection mix was replaced after 6 hours by 293 SFM II suspension medium (Invitrogen, 11686029 ...
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bioRxiv - Biochemistry 2022Quote: ... was used for quantifying the specific transcripts and analyzed with QuantStudio 6 Flex (Life Technologies). Primers used in qPCR analysis are mentioned in sup ...
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bioRxiv - Microbiology 2022Quote: ... and quantified mRNA levels with the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). For each high-ranked and low-ranked HB candidate gene ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using SYBR® Green master mix (Applied Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were seeded in 6-well tissue culture-treated polystyrene plates (Fisher Scientific, Hampton, NH) at 30,000 cells/cm2 for 2 days until cells have recovered in time for treatments ...
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bioRxiv - Cancer Biology 2024Quote: ... melanoma cell lines were seeded in 6 well tissue cultured dishes in RPMI 1640 (Gibco) supplemented with 10% FBS (Biosera ...
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bioRxiv - Neuroscience 2024Quote: ... samples were also counterstained with DAPI (4′,6-diamidino-2-phenylindole; ThermoFisher Scientific, Waltham, MA) to visualize nuclei and/or CellMask (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... qRT-PCR was performed on QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) with 384-well platform ...
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bioRxiv - Physiology 2024Quote: ... Crypts were then suspended in 6 mL of cold Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10 μM Y27632 and 1% PSA ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed 6 times as before and mounted using Prolong Gold Antifade (Thermo Fisher) for confocal microscopy or maintained in PBS at 4°C prior to STORM imaging ...
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bioRxiv - Immunology 2023Quote: ... Using Polycarbonate Cell Culture Inserts (0.4 µm pore) in a 6-well plate (Nunc, 140660), 50’000 Ge518_PTPRZ1-KO cells were seeded in the bottom of the well and 50’000 Ge518_PTPRZ1-KI cells were seeded at the top of the insert ...
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bioRxiv - Cell Biology 2023Quote: ... HepG2 IGF1R KO cells were cultured in 6-well plates and detached with Versene (Gibco) for 5 minutes at 37°C ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with Fast SYBR Green Master mix (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed and stained for 5min with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher) for detection of nuclei and mounted with Fluoromount-G (SouthernBiotech) ...
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bioRxiv - Genomics 2024Quote: ... 1.2 x 106 cells were transfected in 6-well plates using Lipofectamine 3000 (Invitrogen, L3000008) according to the manufacturers instructions with 200 ng pMD2.G (Addgene #12259) ...
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bioRxiv - Genomics 2024Quote: ... with 10ng cDNA per reaction on a QuantStudio 6 Real-Time PCR system (Applied Biosystems). All RT-qPCR reactions were performed in triplicate and outliers were removed if the Ct value measured differed more than 0.5% from the mean ...
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bioRxiv - Genomics 2024Quote: ... with 10ng cDNA per reaction on a QuantStudio 6 Real-Time PCR system (Applied Biosystems). All RT-qPCR reactions were performed in triplicate and outliers were removed if the Ct value measured differed more than 0.5% from the mean ...
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bioRxiv - Immunology 2024Quote: ... 4346906) with a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485691). Quantification of gene expression was calculated as described by Pfaffl et al ...