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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 6 Bromo 3 N ethylamino 1 2 4 triazolo 4 3 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 3-Isobutyl-1-methylxanthine (IBMX) was from Acros Organics (#228420050). PF-04447943 was purchased from MedChem Express (#HY-15441/CS-0942) ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Adenylate Cyclase 3 (IF: 1:500, Invitrogen, PA5-35382); Anti-Ephrin-B1 (IF ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were diluted (1:3) using loading buffer (ThermoFisher), heat-denatured for 3 minutes at 95°C ...
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bioRxiv - Molecular Biology 2019Quote: Cells were pulse-labeled with the thymidine analog Bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrdU; ThermoFisher) for 2 hr (50mM ...
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bioRxiv - Immunology 2024Quote: ... senescent CD4+ T cells were treated with BrdU (5-bromo-2′-deoxyuridine; 10µM; B23151-ThermoFisher) for 72 hours ...
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bioRxiv - Microbiology 2021Quote: ... mixed with forward and reverse detection primers (T3DCD S1 forward [5’- TACGCGTTGATCACGACAAT-3’] and T3DCD S1 reverse [5’- TGGCGAGATTATTCCCTGAC-3’] or GAPDH forward [5’- ACCCAGAAGACTGTGGATGG-3’] and GAPDH reverse [5’- GGATGCAGGGATGATGTTCT-3’]) and SYBR Select Master Mix (Applied Biosystems), and then subjected to PCR using the StepOnePlus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... were blocked for 2 hr at 4 °C in lysis buffer containing 1 mg/ml yeast tRNA (Ambion) and washed twice with 1 ml lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1:4 and 2 μL was used in a 20 μL qPCR Taqman assay (Invitrogen). Assay probes were used to detect cDNA from Nkx2.9 (Mm00435145_m1) ...
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bioRxiv - Neuroscience 2021Quote: ... protein was extracted from 3-week mature neurons using neuronal protein extraction reagent (N-PER) (Fisher Scientific, Waltham, MA) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Biophysics 2022Quote: The human wild-type S1PR5 gene (UniProt ID Q9H228) with an N-terminal 3 × HA epitope (YPYDVPDYA) tag was cloned into pcDNA3.1+ (Invitrogen) at KpnI(5’ ...
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bioRxiv - Bioengineering 2020Quote: ... Proliferation was assessed using a Click-iT EdU Alexa Fluor 594 Imaging Kit (ThermoFisher; n=3/group/time point). EdU was added to media at a concentration of 10 µM at days 2 and 6 of culture and incubated for 24 hours before gels were fixed at days 3 and 7 ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was isolated from ontogenetic and regenerating scales (n=3 fish per group, RU) by using Trizol (Invitrogen) for RNA-sequencing (RNA-seq ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) for 10mins and later incubated with 4 uM fluo-4/AM (1 mM, Molecular probes, #F14201) and 0.002% pluronic F-127 (Sigma ...
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bioRxiv - Physiology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) was used (Invitrogen; 1:5000).
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bioRxiv - Physiology 2022Quote: ... 6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific). The stains were analyzed using a microscope (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... 6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific). The stains were analyzed using a microscope (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... 6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific). The staining was visualized using a microscope (Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (1:10000, Thermo Fisher Scientific). Semi-quantitative methods were utilized for analysis ...
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bioRxiv - Microbiology 2019Quote: ... pneumoniae TIGR4 was detected using 1:2 optimized rabbit type-4 antiserum and 1:100 anti-Rabbit-Alexa-Fluor-488 (Thermo Fisher). Binding of human FH (Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... 1 uM APG-4 (pre-incubated 1:1 with Pluronic-127, Invitrogen) and K+-permeable ionophores valinomycin ...
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bioRxiv - Immunology 2022Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S ‘2P’ and HexaPro IMAC elution fractions were concentrated to ∼ 1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S-2P IMAC elution fractions were concentrated to ~1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Microbiology 2023Quote: ... cells were blocked for 1 h in 3% BSA/PBS and incubated with rabbit-anti-GFP (1:50 in 3%BSA/PBS, 200 µl/well, Invitrogen, G10362) for 1 h at 37 °C ...
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: Pancreas tissue was harvested from wildtype C57BL/6 mice and fixed in 4% paraformaldehyde (Fisher Scientific), cryo-preserved using 30% sucrose ...
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bioRxiv - Microbiology 2023Quote: ... IFN-γ IL-4 and IL-6 were measured by Mouse Uncoated ELISA Kit (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135 ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... A glass coverslip (4×4 mm, #1.5 thickness; Thermo Scientific) was positioned over two-thirds of the craniotomy ...
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bioRxiv - Immunology 2024Quote: ... and 4 ng/ml IL-4 (Fisher Scientific, #404-ML) was added to each well ...
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bioRxiv - Molecular Biology 2024Quote: ... BMP-4 (bone morphogenic protein 4, 25 ng/mL, Invitrogen) and VEGF (vascular endothelial growth factor ...
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bioRxiv - Immunology 2024Quote: ... 25 mM N-2-hydroxy-ethylpiperazine-N′-2-ethanesulfonic acid (HEPES, Gibco, Grand Island, NY, USA), 100 U/mL penicillin and streptomycin (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with following primary antibodies and/or labelling agents: anti-GFP (Invitrogen, #11122, 1:800, 3 days or Invitrogen, #10262, 1:800, 3 days), anti-V5 (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... Round 2 and 3 PCRs were followed in real time using SYBR Green (Invitrogen) and stopped prior to plateauing ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged every 2-3 days by washing with HBSS (Gibco, cat#14170), trypsinizing using 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MMT) was obtained from Thermo Fisher. Crystal violet was purchased from VWR ...
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bioRxiv - Bioengineering 2020Quote: Nbs were diluted in a 2- or 3-fold series in Opti-MEM (Thermofisher). Each Nb dilution (110 μl ...
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bioRxiv - Physiology 2021Quote: ... cells were fed every 2-3 days with DMEM + 10% FBS (Thermo fisher Scientific) until differentiation was complete at day 10.
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bioRxiv - Immunology 2022Quote: ... The band are visualized with gel electrophoresis using 2-3% agarose (#16500500, Thermo Fisher) gel with SYBR Safe dye (#S33102 ...
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bioRxiv - Genomics 2019Quote: ... Round 2 and 3 PCRs were followed in real time using SYBR Green (Invitrogen) and stopped prior to plateauing ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 2-3 sections were mounted on each Superfrost Plus slide (Thermo Fisher Scientific). All sections were air-dried at −20 ° C for 1 hour and afterwards stored at −80 ° C.
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bioRxiv - Systems Biology 2024Quote: ... Cells were passaged every 2-3 days using 0.05% Trypsin/EDTA (Gibco™, 25300062). Cell density was 5x 104 per well for the experiments in the μ-Slide (ibidi ...