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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Peptides were then separated with an Acclaim PepMap RSLC column (75 µm ξ 25 cm, C18 2 µm 100Å, Thermo Scientific) at 0.25 µL/min with a gradient of 4-31% solvent B (80% I ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of each sample was injected into the analytical C18 column (75 µm inner diameter × 50 cm, Acclaim PepMap 2 µm C18 ThermoFisher Scientific) equilibrated in 97.5% solvent A (5% acetonitrile ...
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bioRxiv - Plant Biology 2024Quote: ... 100 µM × 2 cm) and then separated on an Easy-SprayTM PepMap RSLC C18 column (75 µM × 50 cm) (ThermoFisher Scientific) and transferred to the mass spectrometer via an Easy-Spray source with temperature set at 50 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and the trap column was switched on-line to a C18 PepMap Easy-spray analytical column (2 µm, 100 Å, 75 µm I.D. x 50 cm, Thermo Scientific). Equilibration was done in mobile phase A (0.1% formic acid in water) ...
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bioRxiv - Cell Biology 2024Quote: ... experiments were grown overnight in SDC at RT and then diluted in SDC and grown at 30°C for >4 h before being treated with either 500 µM 3-indole acetic acid (3-IAA; AID) for 1h or 1 µM estradiol + 1 µM 5-phenyl-IAA (5-Ph-IAA; Fisher Scientific) for 2h (grAID ...
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bioRxiv - Microbiology 2024Quote: ... equipped with a reverse-phase column (EASY-Spray column, 15 cm × 75 µm ID, PepMap RSLC C18, 2 µm, Thermo Scientific) and a precolumn (Acclaim Pepmap 100 C18 3 µm ...
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bioRxiv - Molecular Biology 2024Quote: ... The 50 µL concentrated media samples were then incubated with 50 µL of 0.1 µm-filtered 1x PBS containing 100 µM Syto RNASelect (Thermo Fisher, S32703) (final concentration 50 µM ...
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bioRxiv - Neuroscience 2024Quote: ... and the trap column was switched on-line to a C18 PepMap Easy-spray analytical column (2 µm, 100 Å, 75 µm I.D. x 50 cm, Thermo Scientific). Equilibration was done in mobile phase A (0.1% FA) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peptides were first loaded on the trap column (Acclaim PepMap 100 C18, 75 µm ID x 2 cm, 3 µm particle size, Thermo Scientific), washed with 0.1 % formic acid in water for 7 min at 5 µL/min and subsequently transferred to the separation column (IonOpticks Aurora C18 column ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides trapped on a 5 mm PepMap 100 C18 column (300 µm ID, 5 µm particle size; Dionex, Thermo Fisher Scientific) and then analyzed on a 200 mm Alltima C18 home-made column (100 µm ID ...
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bioRxiv - Microbiology 2024Quote: ... the flow was directed to an Acclaim PepMap RSLC C18 analytical column (75 µm × 150 mm, C18 reversed phase, particle size: 2 µm; Thermo Scientific). The mobile phase A was 0.1% v/v formic acid in water and the mobile phase B was 0.1% v/v formic acid in acetonitrile ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the sample was loaded on an Acclaim PepMap 100 trap column (100 µm × 2 cm, C18 reversed phase, particle size: 5 µm; Thermo Scientific) using a flow rate of 7 µL/min ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Cell Biology 2024Quote: ... and then were eluted to a C18 column (Acclaim PepMap RSLC, C18, 2 µm, 75 µm x 15 cm, nanoViper; Thermo Scientific) at a flow rate of 300nL/min over 90min ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (Thermo Scientific), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Physiology 2024Quote: ... Analytical separation was then performed at a flow rate of 250 nl/min over a 95 min gradient of 4-25% acetonitrile using a 2 µm EASY-Spray PepMap RSLC C18 75 µm x 250 mm column (ES802A, Thermo Fisher) with a column temperature of 45°C ...
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bioRxiv - Immunology 2024Quote: ... A trap-and-elute method was employed with a C18 PepMap100 trap column (300 µm x 5 mm, 5 µm particle size, Thermo Scientific) and an Acclaim PepMap 100 analytical column (75 µm x 25 cm ...
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bioRxiv - Immunology 2024Quote: ... Peptides were eluted across a PepMap100 C18 nano column (75 µm x 50 cm, 3 µm, 100 Åpore size; Thermo Scientific) at 250 nL/min with buffer A utilising a gradient of buffer B as follows ...
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bioRxiv - Developmental Biology 2021Quote: Membrane fractionations were performed using the Mem-PERTM Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, 89842). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... For membranes with a mixture of CellBrite Cytoplasmic membrane dye (Biotum) and BODIPY FL C5-Ceramide (Invitrogen) at 400- and 1000-fold dilution ...
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bioRxiv - Cell Biology 2021Quote: ... cytosol and membrane fractions were isolated using the Mem-Per Plus Membrane Protein Extraction Kit (89842, Thermofisher) following product guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... slices were placed on polycarbonate membranes for 24-well plates (two slices per membrane; Thermo Scientific #140620). Cultures were allowed to mature for at least 18 days before experimental assessment ...
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bioRxiv - Developmental Biology 2021Quote: ... We stained the plasma membrane using CellMask Deep Red Plasma Membrane Stain (Cat. C10046, Thermo Fisher Scientific) according to the manufacturer’s instructions and fixed the cells with 4% of paraformaldehyde ...
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bioRxiv - Biochemistry 2022Quote: ... and thereafter blotted onto PVDF membranes (Immobilon P membranes, 0.45 μM) according to the manufactures manual (Invitrogen). The membrane blots were blocked with phosphate-buffered saline containing 0.2% Tween-20 (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... cytosol and membrane fractions were isolated using the Mem-Per Plus Membrane Protein Extraction Kit (89842, Thermofisher) following product guidelines ...
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bioRxiv - Immunology 2024Quote: ... Membrane and cytosolic fractions were separate by Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher) following manufacturer’s instruction and immunoprecipitated with 1.5 μg of anti-TRAF1 antibody (clone 1F3 ...
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bioRxiv - Biophysics 2024Quote: ... Cell membranes were stained with 0.1X CellMask™ Deep Red Plasma Membrane Stain (Invitrogen, Cat. No. C10046) at 37 °C and 150 rpm for 10 minutes ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... tissues were embedded in paraffin and serially sectioned at a thickness of 4 um using a microtome (Thermo Scientific Microm™ HM325) and placed on Superfrost Microscope glass slides ...
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bioRxiv - Biophysics 2021Quote: Coverslips for live-cell imaging were prepared by cleaning round glass coverslips (d=25 mm, 0.17 mm thickness) (Menzel Gläser, Thermo Fisher Scientific, Waltham, MA) for 1 min in O2 plasma and incubating them with fibronectin (2 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were sectioned to 7 – 15 μm thickness and deparaffinized before staining with hematoxylin (Catalog No. SDGHS280, Thermo Fisher Scientific, Waltham, MA) and eosin (Catalog No ...
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bioRxiv - Pathology 2022Quote: ... The samples were cryosectioned with a thickness of 12 μm and stained using rapid chrome H&E staining kit (Thermo Scientific, 9990001).
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bioRxiv - Developmental Biology 2022Quote: ... sections were cut in 5 μm thickness and mounted on Superfrost positive charged microscope slides (cat# 12-550-15, Thermo Fisher Scientific). H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698 ...
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bioRxiv - Neuroscience 2021Quote: ... The V1 piece with a total of 1 mm cortex depth by 1.5 mm thickness was transferred to a dish containing 600 µl of RNAlater (Thermo Fisher Cat# AM7020) and kept on ice until dissections were complete ...
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bioRxiv - Microbiology 2020Quote: ... The UHPLC was equipped with a Hypersil Gold C18 column (150 x 2.1 mm, 3 μm film thickness; Thermo Fisher, Waltham, USA) and a C18 guard column (10 x 2.1 mm ...
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bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
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bioRxiv - Immunology 2022Quote: ... 3 ×105 Aag2 cells were seeded on an 18 mm, #1 thickness, poly-D-lysine coverslip (Neuvitro, Vancouver, WA) in a 12-well plate (Thermo Fisher Scientific) and cultured at 28°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue was frozen in TissueTek and sectioned at 35 to 50 μm thickness on a cryostat (Thermo Scientific Microm HM 550) at −20° C ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal or sagittal (15-30 μm thickness) sections were cut with a cryostat and mounted on Superfrost™ Ultra Plus Adhesion slides (Thermo Scientific). Sections were then blocked and permeabilized with a solution containing 3% normal goat serum ...
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bioRxiv - Biochemistry 2022Quote: ... All grids were screened for homogeneous sample distribution and optimal ice thickness on a Glacios cryo-TEM (Thermo Fisher Scientific, Waltham, MA) equipped with a Falcon 3EC direct electron detector at a magnification of 92,000x ...
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bioRxiv - Biochemistry 2024Quote: For cryo-EM data acquisition all grids were pre-screened for optimal particle distribution and ice thickness on an Glacios cryo-TEM (Thermo Fisher Scientific) operated at 200 kV equipped with a K2 Summit direct electron detector (DED ...
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bioRxiv - Biophysics 2023Quote: ... Samples of desired ice thickness were used for data collection in a 300 kV electron-microscope (Titan Krios G3i, Thermo Fisher Scientific) equipped with a cutting-edge direct electron detector (K3 ...
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bioRxiv - Biochemistry 2024Quote: Axle-less TF1: The cryo-EM samples were screened for ice thickness and particle density using a Talos Arctica (Thermo Fisher Scientific) transmission electron microscope (TEM) ...
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bioRxiv - Immunology 2024Quote: ... 10 µm thick axial sections were mounted on Fisherbrand™ Superfrost™ or ColorFrost™ Plus slides (Fisher Scientific, catalog # 12-550-14G and 12-550-18), air dried for 20-60 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Purified RNA was reverse transcribed into cDNA using the high-capacity cDNA reverse transcription kit (Thermo Fisher, 43-688-13). RNA levels were quantified using the IDT PrimeTime Gene Expression Master ...
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bioRxiv - Developmental Biology 2021Quote: Cells were harvested at day 13 of differentiation and the RNA was extracted using Ambion PureLink RNA mini kit (Invitrogen). RT-PCR was performed to obtain cDNA using Superscript III reverse transcriptase and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were stimulated with phorbol 12-myristate 13 acetate (PMA) and ionomycin (Cell stimulation cocktail, eBioscience, ThermoFisher Scientific, USA), and cell supernatants were collected after 24 hours.
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bioRxiv - Immunology 2021Quote: ... mice were administered 150 μg of anti-IL-13 (eBio1316H cat# 16-7135-85) or IgG (eBRG1 cat# 16430185) from Thermofisher. For anti-CCR2 cell depleting treatment ...
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bioRxiv - Neuroscience 2021Quote: ... and imaged or fixed on 13-15 DIV. Neurons were transfected as described in (Sheehan et al. 2016) using Lipofectamine 2000 (ThermoFisher/Invitrogen) on 9 DIV ...
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bioRxiv - Microbiology 2021Quote: ... Purified RNA was reverse transcribed into cDNA using the high-capacity cDNA reverse transcription kit (Thermo Fisher, 43-688-13). RNA levels were quantified using the IDT Prime Time gene expression master mix and TaqMan gene expression Primer/Probe sets (IDT ...
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bioRxiv - Microbiology 2021Quote: ... fixed parasites were incubated with 5µg/mL Blood Group Antigen H (O) Type 1 monoclonal antibody (Invitrogen 13-9810-82), washed three times with 1x PBS and incubated with 1:100 α-mouse secondary antibody conjugated to Alexa 488 (Invitrogen ...