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Citations for Thermo Fisher :
2251 - 2300 of 2879 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... was peeled off from the underlying tissue using a wet sterile cotton swab (7) and placed in Opti-MEM medium (Invitrogen) containing Pen-Strep ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells (ATCC, CRL-1651) and HeLa cells (ATCC, CCL-2) were cultured in high-glucose DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Neuroscience 2023Quote: ... The relative levels of mRNA expression were examined utilizing real-time PCR (Applied Biosystems ViiA 7 Real-Time PCR System) with SYBR® GreenER™ reagent (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: A549 cells encapsulated in hydrogels were stained at the beginning (Day 7) and the end of culture (Day 28) with 2 µM Calcein-AM (Invitrogen) and 30 µg/mL propidium iodide (PI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonads were then dissected in 7% MgCl2·H2O on ice and stored in RNAlater (Thermo Fisher Scientific Inc., Waltham, USA), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... scATAC-seq libraries were amplified with 12 and 7 PCR cycles and quantified with the High Sensitivity dsDNA Quanti-iT Assay Kit (ThermoFisher) using a Synergy HTX microplate reader (BioTek ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Intracellular NO production was assessed during reoxygenation by live-cell staining with the NO-sensitive fluorescent dye 4-Amino-5-Methylamino-2’,7’-Difluorofluorescein (DAF-FM) diacetate (Invitrogen). iPSC-ECs were stained with 1 μM DAF-FM diacetate for 10 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... The killing capacity of the CTL is represented by the % of target cells undergoing apoptosis after 4hr: the co-culture was stained with CellEvent-Caspase3/7-Green (ThermoFisher) for 30mins ...
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bioRxiv - Plant Biology 2024Quote: ... and one 7 mm surgical cotton ball was placed in each well of the 96 well plates (Thermo Fisher scientific). Black plates were used to minimize light reflection and penetration ...
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bioRxiv - Bioengineering 2024Quote: ... 200 μl of antibody or scFv at 1 mg/ml in PBS was mixed with 10% volume of 1M sodium bicarbonate to adjust pH to 7-8 followed by addition of EZ-Link sulfo-NHS-LC-Biotin (ThermoFisher) to reach a 5:1 biotin:protein molar ratio ...
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bioRxiv - Biophysics 2024Quote: COS-7 and HeLa cells as well as their stable cell lines were cultured in DMEM (Gibco, cat. no. 11965092), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... apoptosis was evaluated in NCI-H1975 or HeLa cells using CellEvent Caspase-3/7 Green ReadyProbe Reagent (Invitrogen, Cat#R37111) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: 1 μg of cDNA mixed with 1μl PLUSTM reagent were transiently transfected into DIV 3-7 cultured neurons by Lipofectamine™ LTX (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was performed for both microRNA and genes using the QuantStudio 7 Pro Real-Time PCR System (Applied Biosystems) and TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... They were then transferred to 48-well plates (VTC-48, AS ONE Corp., Osaka, Japan) and cultured for 7 days using OncoPro medium (A5701201, Gibco) supplemented with 1% P/S ...
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bioRxiv - Bioengineering 2024Quote: ... 7–35) were routinely grown as described with high glucose (4.5 g/L) and 1% (v:v) GlutaMax (Thermo Fisher Scientific), supplemented with 10% (v:v ...
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bioRxiv - Biochemistry 2024Quote: ... and 17 µL of 1x RNA Sequencing Buffer (20 mM sodium citrate pH 5, 1 mM EDTA, 7 M urea) (Ambion). The mixture was split across three tubes (9/8/8 µL) ...
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bioRxiv - Immunology 2024Quote: ... Each well was aspirated of residual cell culture media and incubated for 7 minutes at 37°C with 1 mL 1X TrypLE (Gibco) to detach the cells from the plate ...
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bioRxiv - Immunology 2024Quote: ... injection at day 0 and day 7 with 20 µg peanut extract in 100 µL PBS mixed with 100 µL Alum (ThermoFisher). In parallel ...
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bioRxiv - Microbiology 2024Quote: Flow cytometric cell cycle analysis of unstimulated and 4HT-treated P3HR1-ZHT cells was performed using pulsed BrdU incorporation (20 min) and nuclear staining with 7-AAD in fixed cells (Invitrogen eBioscience BrdU staining kit ...
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bioRxiv - Plant Biology 2024Quote: Quantification of ROS in guard cells of epidermal peels was measured using 20 µM 2’,7’-dichlorodifluorescein diacetate (H2DCFDA; Invitrogen) according to Pornsiriwong et al ...
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bioRxiv - Neuroscience 2021Quote: ... Gene expression was determined by qPCR using Brightgreen Dye qPCR master mix on a ViiA 7 Real Time PCR System (Applied Biosystems). Primer sequences were designed using NCBI Primer Blast ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Microbiology 2020Quote: ... The ORFs encoding the MV-C protein and Muc7 protein (Mucin 7 was used as a negative control) were cloned into entry vector pDON207 (Gateway system; Invitrogen, USA). They were transferred into the destination vector pSNL-N2 by Gateway cloning ...
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bioRxiv - Developmental Biology 2021Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems QuantStudio Flex 7 Real Time PCR System (ThermoFisher Scientific). The ΔΔCT method was used for relative quantification of target cDNA abundance representing mRNA expression level normalized to Rn18s (18S rRNA).
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bioRxiv - Cell Biology 2020Quote: ... cells (either MDA MB 231 GFP or a mixture of MDA MB 231 GFP and MCF 7 at a ratio of 1:1) were suspended in cold neutralized bovine collagen solution (2.5 mg/ml) (Thermo Fisher Scientific). This cell suspension was then added to pre-warmed (37 ̊C ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Cell Biology 2020Quote: ... On day 7 the media was switched to repro media2 and E7 reprogramming media (1: 1) containing E6 basal media (Thermo Fisher) with NaB ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was performed using PowerUp SYBR Green Gene Expression Assays (Thermofischer A25741) and a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Relative expression values were calculated using the comparative Ct (ΔΔCt ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Lifetech, #43-676-59) on a ViiA 7 Real-Time PCR System (Applied Biosystems). The primers used are listed in Table S2 ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR reactions were performed from in vitro cultivated samples with 50ng +RT and –RT cDNA using a ViiA 7 Real-Time PCR system (Applied Biosystems) and Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified with Agencourt AMPure XP beads in 0.7:1 ratio (beads to PCR reaction volume) and quantified with the Quant-iT™ High-Sensitivity dsDNA Assay Kit (Invitrogen). The amplicons were pooled together and sequenced on the Illumina HiSeq 2500 using HiSeq Rapid SBS Kit v2 (500 cycles ...
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bioRxiv - Genomics 2020Quote: ... Experiments were performed in biological triplicates per condition and expression was measured in four technical replicates on QuantStudio 7 (Life Technologies) using TaqMan Gene Expression buffer (Life Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Axial and sagittal sections of the tissue at 7–10 μm thick were then processed and mounted onto cold Superfrost Plus slides (Fisher Scientific) by the PALM Histology Core Facility at the University of Ottawa ...
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bioRxiv - Developmental Biology 2022Quote: ... using KAPA SYBR FAST qPCR Master Mix (2X) ABI Prism (Thermo, KK4617) on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) thermal cycler ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio 7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min followed by glycerol in PBS (volume ratio 7:3) for 30 min before being mounted onto superfrost slides (Fisher Scientific) for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Amplicons from the 7 clones that showed loss of a StyI site upon digestion were cloned into Zero Blunt TOPO (ThermoFisher K280020) and sequenced using primers SP6 and T7 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system (Applied Biosystems). Expression of genes was normalised to three housekeeping genes (Gapdh ...
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bioRxiv - Molecular Biology 2021Quote: ... and real-time quantitative PCR was performed using a Viia 7 Real-Time PCR System on 384 custom-made TaqMan microfluidic cards (ThermoFisher, 4342253). CTs were normalised to the average CT values of Gapdh and Hprt ...
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bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures on feeder layers were performed on Mitomycin C-treated MEF as described above with passaging every 7 days using 1 mg/mL collagenase IV in DMEM/F12 (Life technologies) the feeder-free culture was performed on Geltrex™ (Life technologies ...
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bioRxiv - Immunology 2019Quote: ... Then to stain the cells we used antibodies against LFA-1 (clone I21/7) and 4G10 with Alexa Fluor 568–conjugated phalloidin (Life Technologies) and DAPI ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR reactions were performed using a Quant Studio 7 Flex Fast Real-Time PCR System (Applied Biosystems by life technologies) and the following Taqman primers (Thermo Fisher) ...
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bioRxiv - Genomics 2019Quote: ... Gene expression was determined via Taqman qPCR assays (Table S5) and run on the Applied Biosystems ViiATM 7 Real Time PCR system (ThermoFisher Scientific).
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bioRxiv - Systems Biology 2020Quote: ... and the pellet was resuspended in 7 mL PBS supplemented with 0.01 U/μL SUPERase In RNase Inhibitor (AM2696, Invitrogen, Carlsbad, CA), hereafter referred to as PBS-RI ...
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bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR analysis using the comparative Ct method was performed on the Applied Biosystem Vii 7 system (ThermoFisher Scientific) with the Powerup SYBR Master Mix (ThermoFisher Scientific ...