Labshake search
Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... The RT-qPCRs were performed using an ABI550 Real-Time PCR machine (Applied Biosystems, Thermo Fisher Scientific) and the 2x SYBR Green qPCR Mix (Aidlab ...
-
bioRxiv - Plant Biology 2023Quote: ... The RT-qPCRs were performed using an ABI550 Real-Time PCR machine (Applied Biosystems, Thermo Fisher Scientific) and the 2x SYBR Green qPCR Mix (Aidlab ...
-
bioRxiv - Developmental Biology 2023Quote: ... and carried out on an Applied Biosystems Vii 7 RT-PCR system (Thermo Scientific P/N 4453552). Validated gene-specific primers in this study can be found in Table S14 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized using Superscript III First-strand Synthesis System for RT-PCR kit (Invitrogen, 18080-051) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... was retro-transcribed to cDNA using RT-for-PCR kit (Invitrogen, Life technologies, Grand Island, NY, USA). The complete ORFs encoding CpomIR41a1 was amplified combining forward (5’-CACCATGATAATGCCAAGTAAAC-3’ ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... reverse transcription polymerase chain reaction (RT-PCR) was performed utilizing SuperScript III (Thermo Fisher, Waltham, MA, USA). The reverse transcription process occurred at 58 °C for 50 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... RT-qPCR was run using SYBR Green on an ABI ViiA7 real-time PCR system (Applied Biosystems), and results were normalized to the expression of an RPLP0 control primer set ...
-
bioRxiv - Bioengineering 2023Quote: ... RT-qPCR was performed on a Step One Plus Real-Time PCR System (Thermo Fisher Cat#43765592R) with QuantiTect SYBR Green PCR Kit (QIAGEN Cat#204145) ...
-
bioRxiv - Plant Biology 2023Quote: ... RT-qPCR reactions and were performed on a 7500 Fast Real-Time PCR System (Thermo Fisher Scientific). ELF1a was used as a reference gene to normalize transcript abundances ...
-
bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed in triplicate using the PowerUP SYBR Green PCR master mix (Thermo Fisher Scientific) and the Bio-Rad CFX Opus 384 Real-Time RT-PCR systems ...
-
bioRxiv - Immunology 2024Quote: ... using the AgPath-ID™ One-Step RT-PCR kit (Life Technologies, Zug, Switzerland, cat. no. AM1005) with vRNA segment 7-specific oligonucleotide primers and probe (50,51) ...
-
bioRxiv - Physiology 2024Quote: ... We performed RT-qPCR in a 7500 Fast Real Time PCR System (Applied Biosystems, Foster City, CA) using TaqMan Fast Universal PCR Master Mix (Life Technologies ...
-
bioRxiv - Immunology 2024Quote: ... Oligo-dT-primed cDNA was prepared using SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen). Poly(A ...
-
bioRxiv - Cell Biology 2023Quote: ... 10µM forward and reverse primers were used for real time PCR in a StepOnePlus® Real-Time PCR system (all from Applied Biosystems, Waltham, MA, USA). The RT-qPCR amplification protocol comprised a polymerase activation step for 15 minutes at 95 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... with DNase set (ThermoFisher Scientific, 12185010). RNA quality was tested by Agilent Bioanalyzer and confirmed to have RIN numbers > 8.5 ...
-
bioRxiv - Genetics 2019Quote: ... a Foxp3 buffer set (Invitrogen, US) was used according to manufacture instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... and BSA pre-set standards (ThermoFisher) to construct a standard curve ...
-
bioRxiv - Microbiology 2023Quote: ... and dNTP Set (Thermo Scientific R0181), and qPCR was carried out using PowerUp SYBR Green Master Mix (Applied Biosystems A25741) ...
-
Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... and the PureLinkTM DNase Set (Invitrogen). 1000 ng of RNA were converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... RT reaction with SuperScript IV RT (Thermo Fisher Scientific) was performed per manufacture’s recommendations with 150 ng of random primers (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... and transferred to RT tubes (Maxima RT: Life Technologies, Inc. ...
-
bioRxiv - Genetics 2022Quote: ... RT reactions were performed with Maxima RT (ThermoFisher Scientific), while qPCR reactions - with SensiFAST™ SYBR® No-ROX Kit (Bioline) ...
-
bioRxiv - Genetics 2023Quote: ... RT reactions were performed with Maxima RT (ThermoFisher Scientific), while qPCR reactions were performed with SensiFAST™ SYBR® No-ROX Kit (Bioline) ...
-
bioRxiv - Immunology 2021Quote: ... Specific primers were designed with Primer Express 2.0 (Applied Biosystems). The ORF1b primers used were as described before(29) ...
-
bioRxiv - Plant Biology 2020Quote: Primers were designed using the Primer Express 3.0 (Life Technologies). Total RNA was extracted from Arabidopsis leaves using TRIzol reagent (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Primers were designed (Primer Express, Applied Biosystems, Foster City, CA) validated ...
-
bioRxiv - Immunology 2021Quote: ... Specific primers were designed with Primer Express 2.0 (Applied Biosystems). Primer sequences used for mRNA expression were for gene product ...
-
bioRxiv - Neuroscience 2022Quote: ... The primers were designed using Primer Express Software (Applied Biosystems) at exon junctions to avoid genomic DNA amplification ...
-
bioRxiv - Physiology 2022Quote: ... Primers were selected with Primer Express 3.0 software (Applied Biosystems).
-
bioRxiv - Immunology 2023Quote: ... Specific primers were designed with Primer Express 2.0 (Applied Biosystems). The following primer sequences were used:
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primers were designed by Primer Express software (Thermo Fisher Scientific) considering the exon-intron boundaries for all target genes (Table 1) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Primers were designed by Primer Express software 3.0 (Applied Biosystems) based on nucleotide sequences from the National Center for Biotechnology Information data bank ...
-
bioRxiv - Physiology 2024Quote: ... Primers were designed using the Primer Express Software (Applied Biosystems). The TATA box binding protein (TBP ...
-
bioRxiv - Neuroscience 2020Quote: ... A 20 µl PCR reaction was set up in each well using the SYBR PowerUp Green Master Mix (Applied Biosystems, USA) with 10ng of the converted cDNA and 0.3 M primers ...
-
bioRxiv - Immunology 2022Quote: ... The reactions were set up using the manufacturer’s guidelines and run on a StepOnePlue Real-Time PCR Machine (Thermo Fisher Scientific). Values are represented as the difference in cycle threshold (Ct ...
-
bioRxiv - Cell Biology 2019Quote: ... The incubation and cycling conditions were set as described in the kit and the plates were analysed in a StepOnePlus Real-Time PCR System (Thermo Scientific).
-
bioRxiv - Microbiology 2020Quote: ... Reactions were set up in a 384-well plate and run in a QuantStudio 12K Flex Real-Time PCR System (4471081, Applied Biosystems) (2 min at 50C ...
-
bioRxiv - Cell Biology 2021Quote: ... and gene specific primer sets for the core clock genes (Integrated DNA Technologies; see Table S1) on the StepOne Real-Time PCR system (Applied Biosystems). Housekeeping genes were determined by running samples from patients (Human geNorm kit ...
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR reactions were set up in technical triplicates with Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, 11733-046) on a ViiA7 Real-Time PCR System (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... qPCR reactions set-up in a dedicated eDNA PCR hood used TaqMan Environmental Master Mix 2.0 (Applied Biosystems, Waltham, MA, USA). Reagent volumes and cycling conditions are listed in Table 2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... A real-time PCR assay was set up using 25 μL of TaqMan Gene Expression Master Mix (ThermoFisher Scientific, California, USA), 17.8 μL of nuclease-free water (Ambion ...
-
bioRxiv - Bioengineering 2023Quote: ... Relative gene expression qRT-PCR reactions were set up in 10-μL reaction mixtures containing TaqMan Universal Master Mix (Thermo Fisher), the appropriate set of primers ...
-
bioRxiv - Biochemistry 2023Quote: ... A temperature ramp from 5°C to 95°C was set up at a speed of 0.02°C/s on a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific). All measurements were performed in triplicates ...
-
bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR reactions were set up in technical triplicates with Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, 11733-046) on a ViiA7 Real-Time PCR System (Thermo-Scientific) ...
-
bioRxiv - Physiology 2020Quote: ... RT-PCR assays were performed in 384-well plates (Attendbio, Cerdanyola del Valles, Spain) using the 7900HT RT-PCR system (Thermo Fisher Scientific, Waltham, MA, USA) and the commercial reagents Premix Ex Taq™ (BN2001 TaKaRa Bio Inc. ...
-
bioRxiv - Microbiology 2022Quote: ... and quantitative RT-PCR was completed using Power SYBR Green PCR Master Mix in a QuantiStudio™ 5 thermocycler (Applied Biosystems, Foster City, CA, USA) to evaluate the mRNA levels of the following genes using the primers listed in Table 1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription and first round polymerase chain reaction (PCR) were performed using a SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase kit (Invitrogen, Carlsbad, CA, USA). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... Thermo Fisher) and the Applied BiosystemsTM ViiA Real-Time PCR Systems by relative quantitative RT-PCR (Thermo Fisher Scientific, Waltham, MA; Applied Biosystems, Foster City, CA) and expression fold change was calculated using the ΔΔCq calculation method ...
-
bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was then performed using the Applied Biosystems StepOne™ system with SYBR Green RT-PCR Master Mixes (Thermo Fisher Scientific, cat: #A25742). The PrimePCR SYBR green assay primers (see Table 4 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2μg RNA and HvHsfA6a forward and reverse primers were taken for cDNA synthesis and PCR amplification of 1119 bp ORF of HvHsfA6a gene was performed by using one step SuperscriptII RT-PCR system (Invitrogen, Life Technologies, Rockville, MD, USA) according to manufacturer’s instructions ...