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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The RT-qPCR reactions were done using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, USA), 10× diluted cDNA ...
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bioRxiv - Microbiology 2020Quote: ... The viral genomic segments were amplified using the SuperScript IV One-Step RT-PCR system (Thermo Fisher) according to the previously described M-RTPCR protocol 52 ...
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bioRxiv - Cancer Biology 2020Quote: ... Duplicate RT-qPCR assays were carried out in a StepOnePlus™ Real-Time PCR System (Applied Biosystems) with SsoFast™ EvaGreen® supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription was performed using SuperScript III first strand synthesis system for RT-PCR (Invitrogen, 18080-051) according to the manufacturer’s protocol from fibroblasts ...
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bioRxiv - Microbiology 2021Quote: ... The RT-qPCR was performed in a QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific, Waltham, MA) using a Quantabio qScriptTM XLT One-Step RT-qPCR ToughMix kit (Quantabio ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR experiments were performed with the 7500 Fast Real-Time PCR System (Applied Biosystems/Life Technologies). All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR experiments were performed with the 7500 Fast Real-Time PCR System (Applied Biosystems/Life Technologies). All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed in 20 µL reaction volumes containing 1X TaqMan Gene Expression (Applied Biosystems) Assay ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was conducted on BioRad C1000 Touch Thermal Cycler system with Phusion High Fidelity (Thermo Scientific). For RT-qPCR ...
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bioRxiv - Microbiology 2019Quote: ... RT-PCR was performed using SuperScript IV reverse transcriptase and Phusion High-Fidelity DNA polymerase (Thermo Scientific). cDNA was obtained by retrotranscription from RVFV M-segment RNA using primer MRV1ag (5’-CAAATGACTACCAGTCAGC-3’ ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR was carried out in a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA). All reactions were performed using 5× HOT FIREpol EvaGreen qPCR Mix Plus (ROX ...
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bioRxiv - Physiology 2019Quote: ... RT-qPCR was performed on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, Carlsbad, California), with Fast SYBR Green master mix (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... the reaction was carried out by 7500 Fast RT-PCR system (Applied Biosystems, Foster City, CA, USA). Endogenous control was glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Pathology 2020Quote: ... Quantitative real-time reverse transcription PCR (RT-qPCR) was performed with SYBR Select Master Mix (Applied Biosystems) using 10 ng of cDNA and 400 nM forward and reverse primers (Supplementary table 1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-PCR was performed with Power SYBR Green master mix (Applied Biosystems, Foster City, CA) and 50ng cDNA ...
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bioRxiv - Pathology 2021Quote: ... with minor modification to adapt it to the AgPath-ID One-Step RT-PCR Kit (Life Technologies). RT-PCR targets a portion of envelope protein gene (position 26,141-26,253 of GenBank NC_004718) ...
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bioRxiv - Microbiology 2020Quote: ... Semiquantitative RT-PCR was performed with the Quantum RNA™ kit (Thermo Fisher Scientific, Dallas, TX, USA) containing primers to amplify 18S ribosomal RNA (18S rRNA ...
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bioRxiv - Microbiology 2019Quote: ... Isolated RNA (50ug) from cell lines was used as a template for RT-PCR (SuperScript III; Invitrogen). Sequences of interest were PCR amplified from cDNA using Phusion High-Fidelity mastermix (Thermo ...
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bioRxiv - Physiology 2021Quote: ... Quantitative real-time RT-PCR was performed using SYBR Premix Ex Taq with StepOne Plus (Applied Biosystems). The relative levels of Kcc2 were normalized to Gapdh ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... 1-step RT-qPCR was performed using the SuperScript III Platinum One-Step qRT-PCR Kit (Thermofisher) or Luna Universal One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... A one-step RT-qPCR reaction was performed in a StepOnePlus Real-Time PCR System (Applied Biosystems). Cutoff points for Cq values (Cycle of quantification ...
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bioRxiv - Neuroscience 2021Quote: ... pcDNA3.1-Ptprt was cloned from mouse brain RNA by SuperScript III one-step RT–PCR system (Invitrogen). Lentiviral shRNAs against mouse ptprt were constructed in pLKO.1 (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Quantitative reverse transcription PCR (RT-qPCR) was performed using the Universal SYBR Select Master Mix (Applied Biosystems) with a Thermal Cycler Dice TP800 system (TaKaRa) ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the Power Up™ SYBR™ Green Master mix kit (Applied Biosystems) and the following primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative RT-PCR was performed in triplicate wells using Power SYBR® Green Master Mix (Applied Biosystems) with the 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was created using SuperScript IV® First-Strand Synthesis System for RT-PCR (ThermoFisher, Asheville, NC). Resulting samples were quantified on a Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using the SuperScript III Platinum One-Step qRT-PCR Kit (Thermo Fisher Scientific) following the manufacturer’s instructions with the following changes ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed in triplicate using the Power SYBR Green PCR master mix (Thermo Fisher Scientific) and the Applied Biosystems Step One Plus or QuantStudio 6 Flex RT-PCR systems ...
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bioRxiv - Immunology 2020Quote: ... Downstream single cell IgG RT-PCR reactions were conducted using Superscript IV Reverse Transcriptase (Thermo Fisher, # 18090050), random hexamers (Gene Link # 26400003) ...
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bioRxiv - Immunology 2020Quote: ... Virus detection was performed using One-Step RT-PCR SuperScript(tm) III Platinum(tm) Kit (Life Technologies). Thermal cycling was achieved at 55°C for 10 minutes for reverse transcription ...
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bioRxiv - Genetics 2019Quote: ... Synthesis of cDNA was performed with SuperScript III First-Strand Synthesis System for RT-PCR (Life technologies) using random hexamers as primers ...
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bioRxiv - Immunology 2020Quote: ... Expression levels of mRNA were measured with the StepOne Plus RT-PCR system (Applied Biosystems, Burlington, ON), and the comparative Ct method was used to quantify mRNA levels using GAPDH as the normalization control.
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bioRxiv - Immunology 2022Quote: ... and used for cDNA synthesis using the SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen) as described (14 ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was carried out with a Step-One PLUS Fast Real-time PCR cycler (Applied Biosystems) under standard fast cycling conditions using PowerUP SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... RT-qPCR reactions were performed in duplicates on the ABI HT7900 real-time PCR system (Life Technologies). The expression values were calculated including the efficiency of the reactions (49 ...
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bioRxiv - Physiology 2022Quote: ... RT-qPCR reactions were performed in duplicates on the ABI HT7900 real-time PCR system (Life Technologies). The expression values were calculated including the efficiency of the reactions (49 ...
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bioRxiv - Plant Biology 2022Quote: ... The RT-qPCR was carried out on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) using three independent biological samples and three technical replicates of each sample ...
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bioRxiv - Microbiology 2022Quote: Viruses were detected by RT-PCR using a 2720 Thermal Cycler (Applied Biosystems, Foster City, CA, USA) and SuperScript III One-Step RT-PCR System with Platinum Taq DNA polymerase (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR analysis was performed using Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... with the final volume made up with RT-PCR grade nuclease-free water (Thermo Fisher Scientific, Germany). The PCR reactions were conducted in the Bio-Rad CFX96 real-time PCR detection system ...
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bioRxiv - Neuroscience 2022Quote: ... Gene expression levels were measured by RT-qPCR using StepOne Plus Real-Time PCR machine (Life Technologies) with FAM-labeled TaqMan probes (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed in a QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific, Waltham, MA) using a Quantabio qScript XLT One-Step RT-qPCR ToughMix kit (Quantabio ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cDNA was analyzed by quantitative RT-PCR (qPCR) using SYBR green master mix (Life Technologies) on QuantStudio6 Real-Time PCR system (Life Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR reactions were carried out in MicroAmp Fast Optical 96-well reaction plates (Applied Biosystems) using Sybr Green Mix 2x ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated from 25-98°C in a QuantStudio Flex 12 RT-PCR machine (ThermoFisher Scientific). Measurements of fluorescence emission at 570nm were followed for each 0.3°C increment ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed as previously described using the Superscript III First-Strand Synthesis System (Thermo Fisher), iTaq universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR samples were run in duplicate on the StepOne Plus Real-Time PCR System (Applied Biosystems) at 95°C for 10 minutes and 60°C for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription of mRNA was performed using the SuperScriptIII First-Strand Synthesis System for RT-PCR (Invitrogen) with oligo(dT)20 primers ...
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bioRxiv - Developmental Biology 2023Quote: ... and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems). Relative microRNA expression levels were normalized to RNU48 (assay ID ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using Power SYBR® Green PCR Master MIX (Applied Biosystems, Warrington, United Kingdom) and two specific primers (final concentration 500 nM each ...