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Citations for Thermo Fisher :
2001 - 2050 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... blood MZp B-cells were cell-sorted as stated above and lysed with TRIzol solution (ThermoFisher). mRNA was extracted with the phenol/chloroform technique ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Mobile phase B was 0.1% formic acid and 99.9% LCMS-grade acetonitrile (Thermo Fisher, Waltham, MA). The flow rate of the mobile phase was 0.4Lml/min ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used were anti-LC3A/B (rabbit polyclonal; 1: 1000 dilution; Invitrogen, Cat. # PA1-16931), anti-p62 (mouse monoclonal ...
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bioRxiv - Plant Biology 2024Quote: ... and a 0.1% formic acid in acetonitrile (B) (A998-4, Thermo Fisher Scientific, Waltham, MA, USA). The column temperature was 25 °C ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-Caspase-8 (clone B.925.8; RRID:AB_10978471; 0.619g/L Thermo Fisher Scientific Cat# MA5-15226), mouse anti-Caspase-8 (clone 5D3 ...
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bioRxiv - Microbiology 2024Quote: ... To transfer the binding reactions to the nylon membrane (Biodyne™ B Nylon Membrane, Thermo Scientific), we semi-dry transferred the reactions at 15 V for 45 min and immediately crosslinked the transferred DNA to the membrane using a commercial UV-light crosslinking oven (UV Stratalinker ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
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Cellulose fermentation by the gut microbiota is likely not essential for the nutrition of millipedesbioRxiv - Microbiology 2024Quote: ... streptomycin sulfate: 10 µg ml-1 and amphotericin B: 25 µg ml-1 (Thermo Fisher Scientific), following Zimmer and Bartholme (71) ...
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bioRxiv - Biophysics 2024Quote: The cholera toxin subunit B-Alexa FluorTM 488 and 647 conjugates were obtained from Thermo Fisher Scientific and applied to cultured cells at a concentration of 20 nM for a duration of ∼10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The “B” entry clones were constructed by the use of pDONR™ 221 donor vector (Invitrogen) through BP reactions ...
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bioRxiv - Immunology 2024Quote: Cell trace violet/CTV staining of B cells was completed per manufacturer recommended conditions (Thermo Fisher). Enriched splenic B cells (method as above ...
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bioRxiv - Cell Biology 2024Quote: ... the same medium was substituted with fresh RPMI supplemented with B-27™ (Thermo Fisher Scientific). Beating cells usually appeared between days 8 and 12 of differentiation ...
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bioRxiv - Molecular Biology 2023Quote: ... buffer B:100 mM HFIP and 9 mM TEA in LC–MS grade methanol (Fisher Scientific); column ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 20-minute incubation with 1:200 tyramide AF555 (Thermo Fisher Scientific, catalog #: B-40957).
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bioRxiv - Genetics 2023Quote: ... supplemented with the following reagents at the specified final concentrations: B-27 at 1X (Gibco #12587010), GlutaMAX at 1X (Gibco #35050061) ...
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bioRxiv - Immunology 2023Quote: ... the chitin preparation was incubated for 3 h with 10 µg/ml polymyxin B (Thermo Fisher), washed by centrifugation ...
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bioRxiv - Bioengineering 2023Quote: ... The overnight cultures were lysed and sonicated in non-denaturing conditions: B-PER Complete (Thermofisher, 89821), 10 mM imidazole ...
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bioRxiv - Bioengineering 2023Quote: ... Differentiated hiPSC-CMs were maintained in RPMI 1640 supplemented with 2% B-27 (Thermo Fisher Scientific) from day 7 on ...
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bioRxiv - Cancer Biology 2023Quote: ... Lot #4281568) and granzyme B within EMT-6 tumors (Thermo Scientific, Product # PA1-37799, Lot # PA1813162). According to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by primary antibody incubation (Aurora B Kinase antibody; catalog no. A300-431A; Thermo Fisher Scientific) (1:200 in 3% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Hek293 Flp-In T-REx cells were additionally supplemented with 100 µg/ml hygromycin B (Invitrogen) and 10 µg/ml blasticidine (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... the protein expression was induced with 0.2 mM isopropyl b-D-1-thiogalactopyranoside (IPTG, Thermo Scientific) at 16 °C for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... Stably-expressing cells were selected using resistance to Hygromycin B (Thermo Fisher Scientific, Waltham, MA, #10687010) supplied in growth medium at 200μg/mL for 21 days ...
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bioRxiv - Biochemistry 2023Quote: ... cells were chemically lysed for 30 min under mild shaking in B-PER II (Thermo Fisher) diluted to 1x with TEMB buffer supplemented with 0.1 mg/mL lysozyme ...
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bioRxiv - Bioengineering 2023Quote: ... constructs were maintained in Neural Maintenance Media consisting of Neurobasal supplemented with 2.5% B-27(Gibco), 1% Pen-Strep (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: Injections of cholera toxin subunit b conjugated to Alexa Fluor 555 (CTB-555) (Thermo Scientific C34776) was injected at 1X 150-200nl in the secondary motor cortex as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were incubated in primary antibody (Aurora B Polyclonal Antibody (catalog no. A300-431A; ThermoFisher Scientific), diluted in blocking buffer (1:100–1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... and anti-FREM2 human antibody with 77% immunogen identity with zebrafish Frem2a/b (Invitrogen PA5-55986) were used ...
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bioRxiv - Cell Biology 2023Quote: ... Thawed bacterial pellets were lysed in Bacterial Protein Extraction Reagent (B-PER; Thermo Fisher Scientific, 90079) supplemented with 100μg/ml lysozyme (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... with 0.1% formic acid and solvent “B” was 99.9% LC-MS grade acetonitrile (Thermo Fisher Scientific) with 0.1% formic acid ...
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bioRxiv - Physiology 2023Quote: ... anti-human HLA-A,B,C to detect leukocyte derived EVs and Annexin V (ThermoFisher, UK), which binds externalized phosphatidylserine (PS ...
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bioRxiv - Neuroscience 2023Quote: ... while the Neural Medium Plus (NMpl) was supplemented with B-27 plus (Thermo Fisher Scientific, A3582801) instead of the B-27 without Vitamin A ...
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bioRxiv - Immunology 2023Quote: ... Growth medium for BJ-5ta cells was further supplemented with hygromycin B (10 µg/ml, Invitrogen). PBMCs were obtained from healthy blood donors from the University Hospital Essen after informed consent under Ethical Review Board approvals 14-5961-BO and 16-6769-BO ...
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bioRxiv - Microbiology 2023Quote: ... The collected pellets were lysated by chemical lysis using B-PERTM Bacterial Protein Extraction buffer (ThermoFisher). For each gram of pellet ...
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bioRxiv - Neuroscience 2023Quote: ... at 1% B with Solvent A as 0.1% formic acid in water (Thermofisher Optima LS118-4) and Solvent B as 0.1% formic acid in acetonitrile (Thermofisher Optima LS120-4) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 μg/ml amphotericin B (Wisent) and 50 μg/ml gentamicin (Life Technologies Inc., Burlington, ON).
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bioRxiv - Bioengineering 2023Quote: ... cells were cultured in RPMI containing 2% v/v B-27 without insulin supplement (Thermo Fisher) with daily media changes ...
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bioRxiv - Immunology 2023Quote: ... 1 million B cells/well were added to 12-well plates in 1 mL DMEM (Gibco) supplemented with 10% FBS (R&D Systems) ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were collected by centrifugation at 14,500xgxg and lysed in B-PER Reagent (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... iPSC-derived neurons were cultured in BrainPhys Neuronal Media (StemCell) supplemented with 2% B-27 (GIBCO), 10 ng/mL BDNF (PeproTech) ...
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bioRxiv - Systems Biology 2024Quote: ... Mobile phase A and B were 0.1% formic in water (Fisher Scientific, Optima LC-MS grade) and 0.1% formic acid/80% acetonitrile (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Stable clones were selected in DMEM-FBS supplemented with 200 μg/mL hygromycin B (Invitrogen #10687010), 100 U/mL penicillin ...
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bioRxiv - Microbiology 2024Quote: ... and lysed in 100 μL of B-PERTM Complete Bacterial Protein Extraction Reagent (Thermo Fisher Scientific). After heating at 95°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Raji B cells were stained with 1.5 μM carboxyfluorescein diacetate succinimidyl ester (CFSE; #C34554; Invitrogen™) for 8 min at room temperature in PBS and then pulsed with a mixture of staphylococcal superantigens A ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were maintained in NGM (Neurobasal-A Medium, 2% B-27, and 1% Glutamax, from Gibco, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... cell media was replaced with 2 mL/well RPMI supplemented with B-27 minus insulin (Gibco) and 6 μM CHIR99021 (STEMCELL) ...
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bioRxiv - Cell Biology 2020Quote: ... Parasites cell lines were grown in media with 30 µg/mL hygromycin B (ThermoFisher Scientific Cat# 10687010) to maintain the pLEXSY-CK1.2-V5 or the empty pLEXSY plasmids ...
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bioRxiv - Microbiology 2020Quote: ... antibiotics/antimycotics (100 units/mL penicillin, 100 µg/mL streptomycin, 0.025 µg/mL Gibco Amphotericin B, Gibco) and 2 mM L-glutamine (GlutaMAX ...
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bioRxiv - Microbiology 2020Quote: ... antibiotics/antimycotics (100 units/mL penicillin, 100 µg/mL streptomycin, 0.025 µg/mL Gibco Amphotericin B, Gibco) and 2 mM L-glutamine (GlutaMAX ...