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Citations for Thermo Fisher :
1851 - 1900 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The pelleted cells were lysed using B-PER II bacterial protein extraction reagent (Thermo Fisher) for 15 min ...
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bioRxiv - Genetics 2021Quote: ... Immortalized B-cell clonal lines were cultured in Roswell Park Memorial Institute (RPMI) medium (Gibco), containing 15% FBS (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... DCL3 sequences were then sub-cloned into the pFastBac™ HT B vector (Thermo Fisher) and bacmids and baculovirus were produced as previously described (Singh et al. ...
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bioRxiv - Cell Biology 2022Quote: ... cells were co-stained with recombinant Cholera toxin subunit B conjugated to Fluor488 (Invitrogen C34775) at 1 μg/mL for less than 1 minute prior to fixing and staining for NINJ1 as described above ...
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bioRxiv - Neuroscience 2021Quote: ... The “B” entry clones were constructed by using of pDONR™ 221 donor vector (Invitrogen) through BP reactions ...
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bioRxiv - Cell Biology 2022Quote: ... and the cell pellet was resuspended in Neurobasal medium with 2% B-27 supplement (Invitrogen), 1% L-glutamine (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and the resulting bacterial pellet was resuspended in B-PER - Bacterial Protein Extraction reagent (ThermoFisher). Separation of soluble proteins from inclusion bodies was performed by centrifugation at 12,000 x g for 15 min at 4 °C and the insoluble fraction was resuspended in a PBS buffer containing 8M urea ...
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bioRxiv - Neuroscience 2019Quote: ... 10-12 ml of the nonionic detergent-based lysis reagent B-PER (Thermo Scientific #78266) in phosphate buffer containing 1mg/ml of lysozyme (Thermo Scientific #89833) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... gentamicin (50 mg/ml) and amphotericin B (250 µg/ml) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... and in the presence of B-27 supplement (1:100; catalog number 12587010; Thermo Fisher), N-2 supplement (1:200 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cell extracts were prepared with B-PER Complete Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) and the amount of protein was measured with the Bradford Protein Assay (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... the protein and DNA complexes were transferred to biodyne B nylon membrane (Thermo Fisher Scientific) and observed by chemiluminescent detection methods post-UV crosslinking.
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with Alexa fluor 647-cholera toxin B (CTB, 1 µg/ml, Invitrogen) overnight at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... 1x Antibiotic-Antimitotic mix (5000 units of penicillin, 5000μg streptomycin and 15μg Gibco Amphotericin B), 1 X non–essential amino acids and 10 μM all trans-retinoic acid (RA) ...
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bioRxiv - Genetics 2021Quote: ... was added (500 U per tube) together with 5.6 μl 10X Buffer B (ER0211, Invitrogen) for 18 hours digestion at 37 °C with shaking (700 RPM) ...
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bioRxiv - Immunology 2020Quote: ... isolated B cells were stained with Cell Proliferation Dye eFluor™ 670 (5 μM, Invitrogen) and measured after 48 and 96 h.
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bioRxiv - Cell Biology 2020Quote: ... blasticidin and hygromycin B as instructed by the manufacturer (Gibco/Thermo Fisher, Waltham, MA, USA) and gene expression was induced by 1 μg/ml doxycycline ...
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bioRxiv - Cell Biology 2020Quote: ... blasticidin and hygromycin B as instructed by the manufacturer (Gibco/Thermo Fisher, Waltham, MA, USA) and gene expression was induced by 1 μg/ml doxycycline ...
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bioRxiv - Cancer Biology 2021Quote: ... NSB-75 and peripheral blood B cells (PBBC) were cultured in RPMI+10% FBS (Gibco), supplemented with 2mM L-glutamine (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... and transformants were selected on agar media containing 15μg/mL hygromycin B (Invitrogen, Carlsbad, CA).
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bioRxiv - Genomics 2021Quote: ... Cells were selected 2 days post-transduction with Hygromycin B (600 µg/mL, ThermoFisher, 10687010) for 10 days followed by sorting single-cells into 96-well plates with a SH800 sorter (Sony Biotechnology) ...
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bioRxiv - Physiology 2020Quote: ... Solution B contains ∼1% CO2/44 mM + the fluorescent pH-sensitive dye pyranine (H348, Invitrogen), which reports extracellular pH (pHo) ...
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bioRxiv - Plant Biology 2021Quote: ... T1 transgenic plants were selected on plates supplemented with 15 mg/L hygromycin B (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B (Antibiotic-antimycotic solution, ThermoFisher Scientific). Cells were incubated at 37°C in a 5% CO2 environment for the necessary time in accordance with the experiments described below.
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bioRxiv - Cell Biology 2021Quote: ... the media was changed to Neurobasal media with 2% (v/v) B-27 Supplement (Gibco) instead of FBS.
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bioRxiv - Biophysics 2020Quote: ... and 5 mL of B-PER™ complete bacterial protein extraction reagent (Thermo Scientific #89821) with 2 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... MB56 tumor cells were cultured in neurobasal medium supplemented with B-27 (21103-049, Thermofisher). Cells were infected with lentiviral particles of shRNA against Myomegalin or control shRNA.
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bioRxiv - Cancer Biology 2021Quote: ... NHA-PC5 cells were selected on hygromycin B (500 µg/ml; Thermo Fisher Scientific, #10687010) for four days.
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bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... human antibody HLA-A,B,C (1:100, W6/32, #17-9983-42) (Thermo Fisher). To distinguish live/dead cells ...
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bioRxiv - Physiology 2022Quote: ... The cell pellet was then lysed with B-PER buffer (catalog no. 78243, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Stably transfected cells were selected by culturing in media containing hygromycin B and G418 (Thermofisher); cells were cultured in DMEM supplemented with L-glutamine at 2 mM ...
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bioRxiv - Biophysics 2022Quote: ... while anaerobic purification following cell lysis by bead-beating or B-PER (Thermo Fisher Scientific) resulted in complex lacking FdhF ...
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bioRxiv - Neuroscience 2021Quote: ... the mouse ephrin-B3 was cloned into pSecTag-B plasmid (Termo Fisher Scientific, Waltham, USA). Six repeats of the myc epitope were cloned at the carboxyl end of ephrin-B3 ...
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bioRxiv - Neuroscience 2020Quote: ... 3ul 0.2% cholera toxin B subunit (CTB) conjugated Alexa 488 dye (CTB488, Life Technologies, C22841) was intravitreally injected into the left eye ...
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bioRxiv - Immunology 2022Quote: Transduced and sorted B cell lines were stained with Zombie-Violet live dead stain (Thermofisher) and PE-anti-HLA-ABC (Biolegend ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR product was cloned in-frame into pRSET-B protein expression vector (Invitrogen, CA, USA) using In-Fusion®□ HD Cloning Plus (Takara Bio ...
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bioRxiv - Microbiology 2019Quote: ... or after addition of 1 μM Alexa Fluor 647-conjugated Cholera Toxin B (Life Technologies), cells were infected with A ...
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bioRxiv - Immunology 2019Quote: ... Media was aspirated and 100μL of Fix and Permeabilization Medium B (Life Technologies, Madison, W) was added to permeabilize the cell membrane ...
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bioRxiv - Neuroscience 2019Quote: ... cholera toxin subunit B (CTB) conjugated with Alexa Fluor 488 or 555 (Thermo Fisher Scientific) and fluorophore-labeled beads (Green Retrobeads IX ...
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bioRxiv - Immunology 2019Quote: CF996 EBV-transformed B cells were stained with 0.5 μM cell trace violet (CTV, Thermofisher) by incubation at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... TS13-64 cells were grown in DMEM (WELGENE) supplemented with 1X B-27 (Thermo Scientific), 20 ng/mL of bFGF (R&D Systems) ...
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bioRxiv - Cancer Biology 2020Quote: ... B: 20 mM ammonium formate added in 90% ACN (cat No. A998, Thermo Fisher, USA), ammonium hydroxide (cat No ...
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bioRxiv - Microbiology 2019Quote: ... propidium iodide (component B, LIVE/DEAD Bac-Light Bacterial Viability Kit, L-7012, Molecular Probes) was added to the starting inoculum to obtain a final concentration of 20nM ...
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bioRxiv - Neuroscience 2019Quote: ... 300nl of Cholera Toxin B conjugated to AlexaFluor 488 (CTb488, Thermo Fisher, Cat. No: C34775) were microinjected to the right hemisphere NAc as previously (Dong et al. ...
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bioRxiv - Microbiology 2021Quote: ... and incubated in 1x loading solution of LiveBLAzer FRET-B/G Loading Kit (Life Technologies) with CCF2-AM for 1 hour (dark ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested and lysed with B-PER® bacterial protein extraction reagent (Thermo Scientific). The total protein level was checked by the Bradford assay kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... cholera-toxin subunit B (CTB) conjugated with Alexa fluorophore 488 (Thermo Fisher Scientific, Massachussetts, USA), 555 or 647 was unilaterally (left/right hemisphere ...
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bioRxiv - Neuroscience 2021Quote: ... we injected fluorescently-labeled cholera toxin subunit B (CTB, 500 µg / 100 µL, Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Abelson virus-transformed pre-B cell lines were maintained in DMEM (Thermo Fisher #11960-077) supplemented with 10% fetal bovine serum ...