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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Human and murine B cells were lysed in RIPA buffer (Thermo Fisher, #89900) supplemented with protease inhibitors (Roche ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were activated with DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher, 11131D) at a 1:1 cell-to-bead ratio and cultured in 24-well plates with 1 million cells per mL at 37◦C and 5% CO2 overnight.
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bioRxiv - Immunology 2021Quote: ... The remaining cells were enriched for CD8+ cells with Dynabeads™ Untouched™ Human CD8 T Cells Kit (Thermo Fisher #11348D) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: HeLa human cervical cancer cells transfected in conjunction with fluorescence ubiquitination cell cycle indicator (FUCCI) cell cycle sensor (Life Technologies, P36237) were kindly donated by Dr ...
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bioRxiv - Cancer Biology 2023Quote: The total RNA contents of approximately 2 × 106 cells from culture-dissociated tumor cells or human GBM single-cell suspensions were extracted with TRIzol Reagent (#15596026, Invitrogen, USA) and a Direct-zol RNA Miniprep Plus kit (#R2070 ...
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bioRxiv - Immunology 2020Quote: ... Cecal contents were dissolved in fecal immunoglobulin extraction buffer (1X PBS, 0.5%Tween, 0.05% Sodium Azide) containing 1X protease inhibitor cocktail (Cat#78429, Thermo Fisher Scientific, Rockford, USA) and centrifuged at 1500 rcf for 20 minutes at 4°C to isolate the supernatant ...
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bioRxiv - Physiology 2020Quote: ... Sections were then incubated with Alexa Fluor® 488 conjugated goat anti-rat immunoglobulin G (IgG; [H+L]; 1:100, Life Technologies) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were the following: horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG, Invitrogen: G21040, Lot#:2122350, 0.5 μg/ml) and HRP-conjugated goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in PBS and incubated with goat anti-rabbit immunoglobulin G (IgG) (H + L) secondary antibody conjugated with Alexa Fluor 594 (Invitrogen, 1:200). Slides from eWAT were incubated with anti-F4/80 (ab300421 ...
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bioRxiv - Immunology 2023Quote: ... Blood from all animals of an exposure group was pooled and centrifuged (600 x g) and serum isolated and assessed for immunoglobulin E (IgE) and IgG2a using sandwich ELISA (Invitrogen, Waltham, MA) according to manufacturer instructions.
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using Dynabeads Untouched Human T Cells Kit using manufacturer’s protocols (Thermo Fisher, Waltham, MA). Isolated T cells were kept in T cell media ...
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bioRxiv - Microbiology 2019Quote: ... and human monocytic leukemia (THP-1) cells (TIB-202; ATCC) cell lines were maintained in RPMI medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... Human CD8+ T cells were negatively isolated from PBMC and labeled with 5μM of Cell Trace Violet (Invitrogen) according to manufacturer’s specifications ...
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bioRxiv - Immunology 2020Quote: Allogenic CD8+ T-cells were isolated by negative selection using Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen), labeled with carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Biochemistry 2021Quote: ... Differentiation of H9 ES cells was induced by exchanging human ES cell growth medium with DMEM/F12 (Gibco) containing 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: Human proteins (LIS1-SNAP, or motor domains) were expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications3,7,20,69 ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney cells (HEK293T, ATCC, Cat#: CRL-3216) cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Huh7 human hepatoma cells and Calu3 lung adenocarcinoma cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; from Gibco) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2021Quote: Isolation of CD3+ T-cells was performed using Dynabeads® Untouched™ Human T-cells Kit (11344D, Invitrogen). CD3+ T-cells were stained with CellTrace Violet Cell Proliferation Kit (C34571 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs using the Dynabeads® Untouched Human T Cells Kit (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Adherent human glioblastoma cell lines and patient-derived cell cultures were passaged using Trypsin-EDTA (0.25%, Gibco, #25200056). For DRUG-seq ...
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bioRxiv - Biochemistry 2023Quote: Human Embryonic Kidney Cells 293 (HEK293T cell) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Scientific™) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Human monocyte cell line THP-1 cells were cultured in RPMI 1640 medium with added 10% FBS (Gibco) and 1% penicillin-streptomycin (Hyclone) ...
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bioRxiv - Bioengineering 2023Quote: Human lung squamous cell carcinoma cell line NCI-H226 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human lung squamous cell carcinoma cell line NCI-H1975 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Immunology 2023Quote: ... The human cutaneous T-cell lymphoma cell line HUT78 was cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco) with 20% FBS and penicillin-streptomycin 100X ...
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bioRxiv - Bioengineering 2023Quote: Human lung squamous cell carcinoma cell line NCI-H1975 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human lung squamous cell carcinoma cell line NCI-H226 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cancer Biology 2020Quote: ... not commercially obtained but can be provided on request) (42) and HEK293-FT (R70007, Thermo Fisher Scientific) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2021Quote: ... growth media containing DMEM (HEK293) or DMEM/F12 (CHO) (both high glucose, pyruvate, Thermo Fisher Scientific (TFS)) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... and 5% CO2 in DMEM (for HEK293 and HeLa) supplemented with 10% Fetal bovine serum (Invitrogen, Switzerland) and 1% of Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293 (NT and FASTKD5 sgRNAs) samples were purified using the Dynabeads mRNA purification kit (ThermoFisher, 61006) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... platelet-derived growth factor receptor A (PDGFRA) and type I collagen (COL1A1) (Thermo Fisher, Waltham, Massachusetts). Probe references ...
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bioRxiv - Neuroscience 2021Quote: ... surface receptors were labeled with Pierce™ Premium Grade Sulfo NHS-SS-Biotin (Thermofisher, Waltham, USA) and purified using Streptavidin High Performance Spintrap™ (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Receptor and chemokine baculovirus stocks were produced using the Bac-to-Bac Baculovirus Expression System (Invitrogen). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... western blots were performed to normalize for receptor quantity using monoclonal anti-HA antibody (Thermo Scientific). Reactions were started by adding 150μL partially purified receptor samples to 300μL of reaction mixture and incubating on ice for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... and IGF-1 receptor (Catalog number: AM51331, siRNA ID:110754) with Lipofectamine RNAiMax Transfection Reagent (Invitrogen), according to manufacturer’s established protocol ...
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bioRxiv - Neuroscience 2023Quote: Stably expressing 5-HT receptor Flp-In 293 T-Rex Tetracycline inducible system (Invitrogen, mycoplasma-free) were used for calcium flux assays ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... Membranes were probed with antibodies against: estrogen receptor β (Erβ, rabbit, 1:1000, Invitrogen PA1-311), tumor necrosis factor receptor 1 (TNFR1 ...
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bioRxiv - Evolutionary Biology 2024Quote: The chemicals used for the deorphanization of receptors were obtained from Acros Organics (Morris, NJ, USA), Alfa Aesar (Ward Hill ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies used were as follows: anti-mouse aryl-hydrocarbon receptor (ThermoFisher Scientific, MA1-513, 1:500) and anti-β-actin antibody (Millipore Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified using HisPur Ni-NTA Resin (Thermo Fisher). Resin-bound proteins were washed (25 mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and templated using the Ion PI Hi-Q Chef kit (Thermo Fisher). The libraries were sequenced on an Ion Proton system (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Hi-Q reagents and PI™ chip kit V3 (ThermoFisher, Waltham, USA) and the sequencing was performed for 300 flows ...
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bioRxiv - Synthetic Biology 2019Quote: ... Dual immunogold labeling was conducted with 6x-His Tag Monoclonal Antibody (Invitrogen) and the HA Tag Polyclonal Antibody as primary antibodies and an anti-mouse IgG-gold (40 nm ...
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bioRxiv - Genetics 2019Quote: To the amplification products were added formamide (Hi-Di Formamide, Applied Biosystems) and the GeneScan 500 molecular weight standard labeled with the fluorochrome LIZ ™ (orange fluorescence ...