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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Human CD3/CD28 T-cell expansion Dynabeads were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2023Quote: Human cells HEK293T (ECACC 12022001) were cultivated in DMEM (Gibco, Cat.No: 41966) in CO2-incubators (Binder C150 E2 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... HEK293T (human embryonic kidney 293T) cells were incubated in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... A549 human lung epithelial cells (ATCC) were cultured in F12K media (Gibco) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... SW13 human adrenocortical carcinoma cells were cultured in high glucose DMEM (Invitrogen) supplemented with 10% fetal calf serum and antibiotics ...
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bioRxiv - Biochemistry 2024Quote: ... CCR6+ T cells were activated with Dynabeads Human T-activator (Gibco, 11132D) at a density of 1 × 106 cell/ml in growth media (RPMI1640 media with 10% serum ...
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bioRxiv - Immunology 2023Quote: Human HEK293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ∼1 x 106 HEK293 cells grown at 70% confluency on 35 mm dish embedded with 12 mm glass viewing area (Nunc, Thermo Fisher Scientific Inc., Waltham, MA, USA) were transfected with appropriate plasmids using Lipofectamine 3000 ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mg of His-SUMO-SecA purified from untreated cells was bound to 100 μl Ni-NTA agarose beads (Life Technologies). The beads were incubated with 500 μl of lysis buffer or buffer containing 2 mM sodium azide for 10 minutes and washed three times with 500 μl lysis buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The cells after incubation were washed with FACS buffer and incubated with anti-his Alexa Fluor 647 antibody (Thermo Fisher Scientific) for 30 minutes on ice ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher). The resulting enriched library pool was subsequently sorted using a Sony SH800 Cell Sorter ...
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bioRxiv - Immunology 2021Quote: ... HI) containing S gene variants were transfected into Drosophila S2 cells using the Lipofectamine LTX with PLUS reagent (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Microbiology 2022Quote: His-GspD and His-PilQOlut were expressed in Escherichia coli BL21 cells and purified according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... monocytes/macrophages (CD3-CD19-NKp46-CD11b+Gr1loLyC6int/hi) and neutrophils (CD3-CD19-NKp46- CD11b+Gr1+LyC6int) were counted using a Countess II Automated Cell Counter (Thermo Fisher) and then combined and processed as one sample per mouse ...
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bioRxiv - Developmental Biology 2019Quote: ... Medium for H9 and PEN8E-derived organoids was supplemented with 20 ng/ml insulin-like growth factor 1 (IGF1; ThermoFisher Scientific) and 55 nM beta-mercaptoethanol (2-ME ...
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bioRxiv - Molecular Biology 2020Quote: DNA regions corresponding to the coding sequence of each uL18-Like protein but lacking the stop codon were PCR amplified and transferred into pGWB5 (22) by Gateway™ cloning (Invitrogen) to create GFP translational fusions ...
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bioRxiv - Microbiology 2020Quote: ... PCR product (smmA3C-like sample only) from the lowest denaturation temperature was cloned using CloneJET PCR Cloning Kit (Thermo Fisher Scientific) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... the polyherbal formulation was characterized by different spectroscopic techniques like FTIR spectroscopy (Nicolet 6700 FT-IR spectrometer, Thermo Fisher Scientific, USA), 1HNMR spectroscopy (600 MHz ...
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bioRxiv - Genetics 2023Quote: ... The emerging ESC-like colonies were manually picked and transferred into 96-well plates coated with rhLaminin-521 (Thermofisher Catalog#A29248) in mTeSR™ Plus medium (Stem Cell Technologies Catalog #100-0276) ...
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bioRxiv - Neuroscience 2024Quote: ... the eGFP-positive island-like structures within the dorsal striatum of Nr4a1-eGFP transgenic mice were carefully isolated onto LCM Macro Caps (Applied Biosystems) and designated as “patch” ...
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bioRxiv - Cancer Biology 2021Quote: ... the same protocol as above was used except cells were treated with the following antagonists which were directly added to the conditioned media: AZD4547 (FGF receptor, Fisher Scientific #NC0660421, 100 ng/ml) or DMH1 (BMP receptor ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Human pluripotent stem cells (PSCs) were generated from anonymized human PBMCs based on published protocols115 using Sendai viral vectors (Thermo Fisher, A16517) driving high expression of pluripotency markers NANOG and OCT4 ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Biophysics 2019Quote: Jurkat T cells (Human T cell leukemia cell line– ATCC TIB-152) were grown in RPMI-1640 with Glutamax (GIBCO), supplemented with 10 % foetal calf serum and ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Immunology 2020Quote: ... African Green monkey kidney epithelial cells) and Huh-7 cells (human hepatocellular carcinoma cells) were cultured in Dulbecco’s minimal essential medium (DMEM; Life Technologies), supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 (ATCC) were transfected using Lipofectamine LTX® Reagent and OptiMEM Reduced Serum Medium (Thermo Fisher) with pCMV-MAPK14 Wt (DU1765 ...
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bioRxiv - Genomics 2022Quote: HUVECs and pB-circRNA transfected HEK293 were grown in media supplemented with exosome-depleted (Life Technologies). HEK293 were induced with 3 μg/ml doxycycline (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific, Cat. #11995040). pCDH-EF1a-mCherry-EGFP-LC3B (RRID ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa RIEP (Jurkin et al. 2014) and HEK293 Flip-In™ T-REx™ (FITR, Invitrogen) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM high glucose ...
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bioRxiv - Cancer Biology 2021Quote: ... primary antibodies were detected using Alexa Fluor 680 F(ab’)2 fragments of goat anti-mouse immunoglobulin G (IgG) or anti-rabbit IgG (Life Technologies) diluted at 1:8,000 in Blocking buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 200 µl of permeabilization buffer with 10 µg/ml donkey anti-mouse immunoglobulin G–AlexaFluor568 (Thermo Fisher Scientific; Cat. No. A10037), donkey anti-rabbit immunoglobulin G–AlexaFluor647 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cell associated immunoglobulin was detected with an appropriate FITC-labelled secondary antibody and fluorescence measured using an Attune NXT cytometer (Thermofisher, UK). Data are shown as relative fluorescence intensities calculated as a percentage of the negative control fluorescence.
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bioRxiv - Systems Biology 2022Quote: ... Immune complexes were detected with Alexa Fluor Plus 488–conjugated goat antibodies to rabbit immunoglobulin G (Invitrogen–ThermoFisher Scientific, Cat# A32731). Nuclei were stained with Hoechst 33342 (Invitrogen) ...
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bioRxiv - Systems Biology 2022Quote: ... Immune complexes were detected with Alexa Fluor Plus 488–conjugated goat antibodies to rabbit immunoglobulin G (Invitrogen–ThermoFisher Scientific, Cat# A32731). Nuclei were stained with Hoechst 33342 (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used are: ECL anti-mouse immunoglobulin G (IgG) horseradish peroxidase linked whole antibody (1/10 000; Fisher Scientific; NXA931V) for anti-MAP1B ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (Alexa Fluor 488 donkey anti-rabbit or Alexa Fluor 647 donkey conjugated to anti-goat immunoglobulin G (H+L) (Life Technologies). The coverslips were then mounted with DAPI (diamidino-2-phenylindole)-free Fluoro-Gel on glass slides (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... myelin debris26 and immunoglobulin G-opsonized red blood cells42 (IgG-RBCs) were labelled with pHRodo GreenTM STP ester (Thermo Fisher Scientific) and were used at the following respective concentrations which were determined to be non-saturating ...
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bioRxiv - Cancer Biology 2023Quote: ... Horseradish peroxidase– conjugated secondary antibodies, goat anti-mouse immunoglobulin G (IgG) (# 32230), and goat anti-rabbit IgG (# 32260, 1:5000) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... donkey anti-mouse or Alexa Fluor 568-conjugated (A11036) or Alexa Fluor 488-conjugated (A11034) donkey anti-rabbit immunoglobulins (Molecular Probes, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated for 1 h at RT with the secondary antibodies: goat anti-rabbit immunoglobulin G (IgG) Alexa Fluor 488 and/or goat anti-mouse IgG Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed three times in PB and incubated at room temperature with the secondary antibody to rabbit immunoglobulins (Thermo Fisher Scientific Cat# A21206 ...
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bioRxiv - Physiology 2024Quote: ... New Zealand) was used in combination with AlexaFluor 647-conjugated goat anti-guinea pig immunoglobulin (1:500, Thermo Fisher Scientific, USA). For GnRH dendron immunostaining ...
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bioRxiv - Bioengineering 2019Quote: ... 10x of mouse (for NIH 3T3 cells) or human (for HCT116 cells) Cot-1 DNA (Thermo Fisher Scientific Cat ...