Labshake search
Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc ...
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bioRxiv - Biochemistry 2023Quote: ... BODIPY-CE (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3- dodecanoate) was obtained from Life Technologies. Lipids (with the exception of NBD-LPC ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... 3-4 days post-nucleofection (depending on cell confluency) selection was started using 400 ng/mL Puromycin (ThermoFisher A1113802) in FM (PFM) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 3-4 days during the log phase of growth with Trypsin/EDTA solution (Gibco, R001100) following a PBS wash ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse cochleae were fixed in 4% PFA for 3 hours and microdissected in cold 20% RNAlater (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... dried and cover slipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Slides were imaged at 10X magnification on a slide scanning microscope Olympus VS120 slide scanning microscope (Olympus ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of 1 mg mL−1 was vitrified using a Mark IV Vitrobot (Thermofisher). Prior to sample vitrification ...
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bioRxiv - Immunology 2022Quote: ... containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies or reagents were used ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with 4-6 μg linearized targeting vector DNA (ROSA26-SpCas9) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, USA), selected with 1400-1600 μg/mL G418 (Genaxxon Bioscience ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from liver tissues of SHR and WKY (4-6 weeks old) using the TRIzol reagent (Invitrogen, Waltham, USA) followed by purification using Nucleospin columns (Machery-Nagel ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with PBS and mounted on the slide with ProLong Diamond antifade with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies, #P36966). Confocal images were acquired on a Nikon Ti-Eclipse A1M microscope fitted with a 60× oil immersion objective using 488 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 × 105 of isolated MLEC were cultured in a 4-well chamber slide (Nunc Lab-Tek Chamber Slide System, Thermo Fisher Scientific) precoated with Extracellular Matrix Attachment Solution provided in the kit ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were washed three times and ProLong® Gold Antifade mounting reagent containing DAPI (4’,6-diamidino-2-phenylindole) (Thermo Scientific, #P10144) was applied to mount the coverslips.
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bioRxiv - Microbiology 2021Quote: ... then 50 μl of the EV preparations were mixed with 250 μl PBS staining buffer containing 2.5 µg/ml DAPI (4’, 6-diamidino-2-phenylindole; Thermo Fisher Scientific, USA)) and kept at 4 °C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Genetics 2020Quote: MEFs were fixed on 6-well glass bottom dishes with 4% PFA (diluted from 16% stock in PBS; ThermoFisher Scientific, Massachusetts, US), washed with PBS ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were counterstained with 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI) and appropriate secondary antibodies conjugated to fluorochromes (Thermo Fisher Scientific) were applied for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells were typically transfected in a 6 well plate format with 20 μM siRNAs and 4 μL Lipofectamine RNAiMAX (Thermo Fisher Scientific); the depletion phenotype was inspected 72 h after transfection unless otherwise indicated in the text or the legends ...
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bioRxiv - Immunology 2020Quote: ... Isolated B cells were stained for 20 minutes on ice with a fluorescence staining-mix containing 4’,6-Diamidin-2-phenylindol (DAPI; Thermo Fisher Scientific), anti-human CD20-Alexa Fluor 700 (BD) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and filamentous actin with Alexa Fluor 488-conjugated phalloidin (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific, R0533) diluted in serum-free media ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were then washed with 1x PBS and incubated with μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies, #D1306), followed by mounting as described above.
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bioRxiv - Immunology 2022Quote: ... Bacteria were harvested after 16–18 h at 3000 x g for 15 min at 4°C (Sorvall LYNX 6000 centrifuge, F9-6×1000 LEX rotor, Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Cells were then washed with DPBS before being stained with 50 μL/well of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) (Invitrogen, cat. #D1306) diluted to 5 μM in DPBS for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... After 2 washes with 1xPBS, the cell nuclei were stained with NucBlue Fixed Cell Stain ReadyProbes Reagent (4’,6-diamidino-2-phenylindole, DAPI) (Thermo Fisher Scientific) for 30 min in the dark at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sciatic nerves of 6 mouse pups between postnatal days 2 and 4 (P2 and P4) were collected in L15 medium (Thermofisher, Massachusetts, USA) and incubated for 30 min in 2 mg/mL collagenase II and then 10 min in 0.25 % trypsin containing EDTA at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... the coverslips were washed with PBS three times before being mounted using ProLong™ Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies). Images were obtained with Olympus BX61 fluorescence microscope or Zeiss LSM800 laser confocal scanning microscope and processed using cellSens imaging software (Olympus ...
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bioRxiv - Microbiology 2023Quote: ... After the secondary antibody was washed three times for 5 min, nuclei were stained with DAPI (4’,6- Diamidino-2-Phenylindole, Dihydrochloride) (#D1306, Thermo Fisher Scientific) (dilution 1:1,000 in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were stained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min and mounted using SlowFade Diamond Antifade Mountant with DAPI (Invitrogen, Cat# S36964). Slides were imaged using a Nikon Y-FL microscope attached to a Nikon DS-Qi2 camera and images were captured using NIS elements AR software ...
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bioRxiv - Plant Biology 2023Quote: ... 250 ng/ml 4′,6-diamidino-2′-phenylindole dihydrochloride (DAPI) or 500 nM SYTOX Orange (Life Technologies, Thermo Fisher Scientific, Schwerte, Germany) were used as nucleic acid stain.
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bioRxiv - Microbiology 2022Quote: ... in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
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bioRxiv - Microbiology 2023Quote: ... Fixed cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) for visualization of host-cell nuclei and anti-MOMP antibody conjugated to FITC (Thermo Scientific™) for visualization of Chlamydia ...
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bioRxiv - Genomics 2023Quote: ... nuclei were counterstained with ProLong Gold anti-fade reagent with 4’,6-diami-dino-2-phenylindole (DAPI) (Life Technologies, catalog no. P36935). Images were viewed on a Zeiss Axioskop 2 using AxioVision Rel ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Neuroscience 2023Quote: ... Nuclei was then isolated using a sucrose gradient and ultracentrifugation at 13,000 rcf for 45 mins at 4°C (Sorvall WX-100, F65L-6 x 13.5 rotor, Thermo Fisher, Waltham, MA USA). Isolated nuclei were resuspended in Nuclei PURE Prep kit storage buffer and RNAase inhibitor ...
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bioRxiv - Microbiology 2024Quote: ... The coupon was incubated in a 300 nM (100 ng/mL) solution of DAPI (4’,6-diamidino-2-phenylindole, ThermoFisher, Waltham, MA) for 3 minutes as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated for 5 min with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Dojindo, D523) and then mounted with PermaFluor (Thermo Fisher Scientific). Images were acquired using a BC43 or LSM800 instrument equipped with a Zeiss Axio Observer Z1 and a LSM 800 confocal unit with Airyscan module ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were co-stained with Draq5 (Cell Signal, 4048L) or nuclei stain 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI; Thermo Fisher, D1306). Stained samples were imaged using an Olympus FV3000 Confocal Laser Scanning Microscope.
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bioRxiv - Genetics 2021Quote: ... 6-VIC ® or 6-PET® (Life Technologies Corporation, Carlsbad, USA). During capillary electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... in a 3:1 ratio with 1X penicillin/streptomycin (Gibco; 15070) and 5% FBS (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... rat Taste receptor type 1 member 3 (Tas1r3, Rn00590759_g1, Applied Biosystems) and rat Taste receptor ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... in Fibroblast medium (3:1 DMEM: Ham’s F12 Nutrient Mixture, Gibco) supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were counterstained with ToPro-3 (1:1000 dilution; Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... and washed 3 times with 1x PBS (Fisher Scientific, BP399-1).
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bioRxiv - Microbiology 2020Quote: ... and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000 dilution, Invitrogen). Secondary antibodies against rabbit and mouse IgG conjugated to IRDye 680LT or IRDye 800CW were obtained from Li-Cor (1:10,000 dilution) ...
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bioRxiv - Cancer Biology 2022Quote: ... BxPC-3 and PSN-1 were cultured in RPMI1640 (Thermo Fisher), supplemented with 10% FCS and 1% P/S ...