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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Actinomycin D (ThermoFisher) was used to label dead cells ...
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bioRxiv - Immunology 2024Quote: ... Actinomycin D (Invitrogen) was added to label dead cells ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
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bioRxiv - Biophysics 2020Quote: ... 0.1 M NaCl and 1% n-Dodecyl β-D-maltoside (DDM, Anatrace, Affymetrix) and stirred for 18 hours for solubilization ...
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bioRxiv - Cancer Biology 2019Quote: ... or a non-targeting siRNA pool (Pool #1, D-001206-13, ThermoFisher Scientific). Efficiency of mRNA depletion was assessed 72 hours post-transfection using qPCR or western blot in the case of ACSL1 (Proteintech ...
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bioRxiv - Bioengineering 2021Quote: ... Recombinant protein expression was triggered using 0.5mM isopropyl β-D-1-thiogalactopyranoside (ThermoFisher) and incubated for 18 h in an orbital shaker at 18 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were as follows: HuC/D (mouse, ThermoFisher A21272, 1:200), Cherry (goat ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-HuC/D (1:500; Cat#: A-21271, RRID:AB_221448; Thermo Fisher Scientific), rabbit anti-Krt5 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal IgG2b anti-HuC/D (Invitrogen Thermo Fisher, A-21271, 1:250), Mouse monoclonal IgG1 anti-Phox2b (B-11 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal IgG2b anti-HuC/D (Invitrogen Thermo Fisher, A-21271, 1:250), Mouse monoclonal IgG1 anti-Phox2b (B-11 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.5% w/v Peptone (Formedium) and 1% w/v D-Glucose (Fisher Scientific). For solid medium ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and stained with 7-Aminoactinomycin D (7AAD) (Invitrogen, 1:400) that was used as dead cell marker ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
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bioRxiv - Immunology 2021Quote: ... were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads, Life Technologies) at 1 bead ...
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bioRxiv - Neuroscience 2020Quote: ... treatment one-hour after plating in cell media (DMEM/F12 [Gibco, 10565018], 1% N2 [Gibco, 17502048], 1% penicillin/streptomycin [Gibco, 1570063]). Vehicle treatment in each experiment consisted of equal volume of either HBSS or DMSO (final concentration ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for one hour in primary antibody NPM1 (FC-61991, ThermoFisher, 1 : 500 dilution in PBST with 1 %BSA) ...
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bioRxiv - Plant Biology 2023Quote: ... Chlorophyll content was then measured using a NanoDrop™One/One C Microvolume UV-Vis Spectrophotometer (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... One-step RT-qPCR was performed using the SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) and custom PrimeTime qPCR Probe Assays (IDT ...
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bioRxiv - Immunology 2022Quote: ... containing 100 µl of tempus RNA- stabilizing solution aliquoted from a regular-sized tempus tube (designed for the collection of 3 ml of blood and containing 6 ml of solution; ThermoFisher, Waltham, MA, USA). This method is described in detail in an earlier report (7) ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from hind limb muscle of adult extensor digitorum longus (3-6 months) or newborns (P0) with TRIzol reagent (Thermo Fisher Scientific, 15596026) (41 ...
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bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... and (3) 110,000 × g for 2 hours at 4°C with a Sorvall Discovery SE ultracentrifuge (Thermo Fisher Scientific) with an AH-650 rotor (k factor ...
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bioRxiv - Immunology 2021Quote: ... Excess liquid was blotted for 3 seconds with a force of -4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Genomics 2020Quote: ... Two TaqMan probes targeting the MLLT3 exon 3-4 and exon 9-10 splice junctions (ThermoFisher, Hs00971092_m1 and Hs00971099_m1) were used with cDNA diluted 1:5 in dH2O to perform qPCR for six replicates per sample on an Applied Biosystems Quant Studio 6 Flex ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781; 1µg/ml) labeling was added from the second day to show the whole brain morphology ...
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bioRxiv - Synthetic Biology 2021Quote: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) was supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were subcultured every 3-4 days as follows: cells were washed with phosphate buffered saline (PBS, Life Technologies) and then incubated with trypsin (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... RT reactions were performed with 3-4 µg total RNA using oligo-dT and SuperScript III RT (ThermoFisher Scientific). QPCR was performed as previously described [20] ...
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bioRxiv - Cell Biology 2019Quote: ... Liposomes containing PtdIns(4)P- or PtdIns(3)P were independently labeled with DiI and DiD (Thermo Fisher Scientific) lipid dyes and detected by excitation at 561 nm and 640 nm ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Media was changed every 3 to 4 days and cells were passaged at nearly 90% confluence using 0.25% trypsin (Gibco). B16F0 cells were verified for mycoplasma contamination at Charles River Laboratories for cell line testing ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Neuroscience 2022Quote: ... The craniotomy was then sealed with two glass coverslips (3 mm and 4 mm diameter, Thermo Fisher Scientific, UK) glued to one another using optical adhesive (Norland ...
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bioRxiv - Physiology 2022Quote: ... Cells were fixed with ice-cold 4% paraformaldehyde solution and cell nuclei were stained with To-Pro-3 (Invitrogen). Samples were mounted with Mowiol (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS 3 times then fixed with 4% paraformaldehyde (PFA) in 1x PBS (Thermofisher, cat#043368.9M) at room temperature for 15 min in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... BODIPY-CE (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3- dodecanoate) was obtained from Life Technologies. Lipids (with the exception of NBD-LPC ...
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bioRxiv - Biochemistry 2022Quote: ... Acly-/- MEFs were maintained at <80% confluence (passaged every 3-4 days) in high glucose DMEM (Gibco #11965-084) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells at passage 3-4 were seeded in to black walled clear bottomed 96 well plates (Pierce, ThermoFisher Scientific) at a density of 7,500 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc ...