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Citations for Thermo Fisher :
151 - 200 of 3707 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The trimeric RhGB07 spike protein was expressed as previously reported as for the SARS-CoV-2 spike transiently expressed in suspension-adapted ExpiCHO cells (Thermo Fisher) in ProCHO5 medium (Lonza ...
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Biochemistry 2023Quote: ... The pET24b Cd-IF1 plasmid was subsequently transformed into One Shot™ BL21(DE3) Chemically Competent Escherichia coli (E. coli) (Thermo Fisher Scientific) for expression of the recombinant proteins.
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bioRxiv - Microbiology 2022Quote: ... Transformations were performed in Top10 competent bacteria (One Shot TOP10 chemically competent E. coli, Invitrogen) and selected using 100 µg/mL ampicillin or/and 34 µg/mL chloramphenicol (Euromedex) ...
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bioRxiv - Molecular Biology 2019Quote: ... polyA-tailed using Escherichia coli poly(A) Polymerase (E-PAP) and ATP (Thermo Fisher Scientific), and recovered using a MEGAclear kit (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cell lysate was prepared from the RNAse E deficient BL21 STAR (DE3) (ThermoFisher Scientific) cells that were transformed with the pRARE vector from BL21 Rosettta (Merck) ...
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bioRxiv - Systems Biology 2020Quote: ... and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375, Thermo/Invitrogen), according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2022Quote: ... or SARS-CoV-1-d28 (Genbank AAP13567) or SARS-CoV-2 VOC spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Immunology 2021Quote: ... HIV-based lentiviral pseudotyped particles harboring the SARS-CoV-2 Spike protein variants were produced using the ViraPower Lentiviral Expression system (Thermo Fisher Scientific) according to manufacturer’s protocol with minor changes ...
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bioRxiv - Immunology 2023Quote: ... The cells were then transfected with ancestral or variant SARS-CoV-2 spike protein encoding plasmid using Lipofectamine 3000 Transfection Reagent (L3000001; Thermo Fisher Scientific). After 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (SouthernBiotech ...
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bioRxiv - Microbiology 2022Quote: ... The presence of recombinant virus was verified by immunostaining with a monoclonal antibody against the RABV nucleoprotein (FujiRebio, Cat# 800–092) and polyclonal antiserum against the SARS CoV/CoV-2 S1 domain (Thermo Fisher, Cat# PA581798). The filtered virus was then used to inoculate Vero cells seeded in Cellstack Culture Chambers (Corning ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Microbiology 2020Quote: ... Transformations were performed in Top10 competent bacteria (One Shot™ TOP10 Chemically Competent E. coli, Invitrogen) and selected using 100 μg/mL ampicillin or 50 μg/mL kanamycin sulphate (Euromedex) ...
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bioRxiv - Cell Biology 2021Quote: Ocn-GFPTopaz mice were injected intravenously with 50mg/kg of pHrodo labeled E-Coli particles (Invitrogen) and one-hour post injection mice were sacrificed ...
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bioRxiv - Cell Biology 2022Quote: ... LPS (E. coli 0111:B4, Ultrapure, tlrl-3pelps, used to treat cells) were obtained from Invitrogen. ATP-Na+ (A2383) ...
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bioRxiv - Molecular Biology 2021Quote: 2 × 20 μg of each of the three different SARS-CoV-2 spike proteins were mixed with 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and up to 10 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 E sgmRNA standards were generated by in-vitro transcription of the generated SARS-CoV-2 E sgmRNA cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... After transformation in bacteria (One Shot™ TOP10 Chemically Competent E. coli; Thermo Fisher Scientific, Bleiswijk, Netherlands), successful cloning was validated by sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... or with Pierce™ Glycoprotein Staining Kit (Thermo Scientific) after separation.
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bioRxiv - Microbiology 2020Quote: ... and S1 nuclease (Invitrogen). Total RNA was extracted from the pulverized samples using the TRIzol Plus RNA Purification Kit (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... S1 nuclease (ThermoFisher #18001016) at 20 U/ml in S1 nuclease buffer and 50 mM NaCl was added to one of the two slides and incubated for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Biochemistry 2020Quote: MBP-tagged (pTHMT) full-length SARS-CoV2 nucleocapsid protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA levels were quantified by quantitative reverse-transcriptase PCR (qRT-PCR) specific for the SARS-CoV-2 E gene (23) using TaqMan Fast 1-Step Master Mix (Applied Biosystems) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... cRNA was prepared according to the manufacturer’s instructions from linearized plasmids encoding SARS-COV2 Protein E using the mMESSAGE mMACHINE T7 kit (Ambion, TX, USA). RNA concentrations were quantified by UV spectroscopy (NanoDrop ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 Spike RBD, His, Avitag™ (ACRO Biosystems, SPD-C82E9) immobilized on paramagnetic beads (Dynabeads M-280 streptavidin; Invitrogen Dynal AS, Oslo, Norway) as target antigen ...
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bioRxiv - Immunology 2021Quote: ... The proteins were transferred to nitrocellulose membrane and probed with polyclonal rabbit serum against SARS-CoV S1 domain (Thermofisher, Cat No. PA5-81798 and PA5-81795), monoclonal antibodies I1 (8G5F11 ...
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bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
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bioRxiv - Developmental Biology 2019Quote: ... ERCC spike-in (Invitrogen) were added in the lysis buffer (1:1000000 ...
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bioRxiv - Cancer Biology 2024Quote: ... ERCC spike-in (Invitrogen) was added at a ratio of 2uL of spike-in per 1ug of RNA.
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bioRxiv - Molecular Biology 2021Quote: ... (2020; Figure S1; Life Technologies). The construct was cloned into pGEM®-T Easy (Promega Corp. ...
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bioRxiv - Microbiology 2021Quote: ... S1 Nuclease (Thermo Fisher Scientific) digestion was subsequently performed to de-branch chimeric DNA formed during the rolling circle amplification process ([5]) ...
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bioRxiv - Microbiology 2020Quote: ... S1 nuclease (ThermoFisher Scientific, EN0321) treatment was carried out after inactivation of protease with 0.56 u/µl nuclease in S1 nuclease buffer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 10 units S1 Nuclease (Invitrogen) in S1 Buffer (30 mM sodium acetate pH 4.6 ...
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bioRxiv - Cell Biology 2023Quote: ... S1 nuclease (Invitrogen cat # 18001016) was diluted 1/100 in S1 buffer and used at 20 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... for 5 min and hybridized with smFISH probes diluted 1:3000 in hybridization buffer (10% dextran sulfate, Sigma D8906, 20% formamide, 1 mg/ml E. coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Immunology 2019Quote: ... adult females aged 6 to 11 days after eclosure were injected with fluorescent bioparticles (E. coli K-12 Strain Bioparticles TexasRed Conjugate; Invitrogen) or pH sensitive fluorescent bioparticles (pHrodo E ...
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bioRxiv - Microbiology 2019Quote: ... and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (Avanti Polar Lipid). LTA (Lipoteichoic acid from S. aureus) and LPS (lipopolysaccharide from E. coli) (both obtained from Invitrogen) were also tested ...
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bioRxiv - Cell Biology 2023Quote: ... differentiated monocytes were preincubated for 30 mins with PBS or 25 μμM Cytochalasin D and then incubated with pHrodo red E coli bioparticles (Thermofisher) with or without 25 μM cytochalasin D for 1 h 30 mins ...