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Citations for Thermo Fisher :
351 - 400 of 3707 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... then digested with S1 nuclease (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... Hydrolysis probes (Table S1) were ordered from ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... and then treated with S1 endonuclease (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... ERCC spike-in RNAs (4456653; ThermoFisher Scientific) were added at a 1:40 dilution of stock at 1µl per embryo (5 embryos and 5 µl diluted ERCC RNA per sample ...
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bioRxiv - Genomics 2020Quote: ... ERCC RNA Spike-In Mix 1 (Invitrogen), and the mCherry and EGFP open reading frames from the FUCCI plasmid (we included the latter to ensure that the transgene was being transcribed) ...
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bioRxiv - Neuroscience 2020Quote: ... and ERCC Spike-ins (ThermoFisher Scientific 4456740) diluted at 1:2.4×107 ...
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bioRxiv - Bioengineering 2020Quote: ... 1:600,000 diluted ERCC spike-in (ThermoFisher), and 32 µL of 10% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... and ERCC spike in (Thermo Fisher #4456740) was added ...
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bioRxiv - Microbiology 2021Quote: ... ERCC RNA Spike-In Control Mixes (Ambion) was added proportionally to the total RNA of each sample (0.1 µL per sample ...
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bioRxiv - Cell Biology 2022Quote: ... ERCC spike ins (ThermoFisher cat. num. 4456739) were added to each sample in a dilution ranging from 1:1000 to 1:10000 depending on the initial amount of RNA extracted.
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bioRxiv - Biochemistry 2022Quote: ... ERCC RNA Spike-In Mix (both Invitrogen) was added according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1:4000000 ERCC spike-in (Ambion, 4456740), 2.5 mM dNTPs (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... and ERCC spike-in synthetic RNAs (Ambion). 96-well plates were snap frozen and stored at –80 °C until use ...
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bioRxiv - Immunology 2020Quote: ... and ERCC RNA spike in controls (Ambion)) in a 96-well V-bottom PCR plate (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: ... ERCC ExFold RNA Spike-in mix (Invitrogen) was added to purified RNA ...
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bioRxiv - Cell Biology 2019Quote: ... ERCC Mix 1 spike-in controls (ThermoFisher) were added at 1e-5 final dilution ...
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bioRxiv - Microbiology 2021Quote: ... ERCC RNA Spike-In Control Mix (ThermoFisher) was added into each sample following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... ERCC spike-in controls (Ambion, Austin, TX) were added on a per-cell basis for downstream normalization ...
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bioRxiv - Systems Biology 2023Quote: ... ERCC ExFold RNA Spike-in mix (Invitrogen) was added to purified RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... ERCC RNA Spike-In Mix II (Invitrogen) was added in 1:50 x 103 ratio to each of the wells administered in 150 nL volume by the micro-dispenser Nanodrop II (BioNex) ...
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bioRxiv - Plant Biology 2020Quote: ... NAA50 was cloned into the cauliflower mosaic virus 35S promoter vector pEarleyGate100 (Earley et al. 2006) using a modified multisite Gateway recombination cloning system (Invitrogen) as described in (Qi et al ...
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bioRxiv - Plant Biology 2021Quote: ... ROMT2 and ROMT3 were transferred into the binary destination vector pB2GW7 including Cauliflower mosaic virus (CaMV) 35S promoter through Gateway LR clonase recombination (Invitrogen). Together with ROMT1 and STS48 constructs (Schmidlin et al. ...
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bioRxiv - Biophysics 2023Quote: Plasmids for mosaic experiments such as Actin-GFP or Caldesmon-GFP were incubated with cells using Lipofectamin (Invitrogen, cat. 11668030). One day prior experiment ...
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bioRxiv - Cell Biology 2022Quote: ... and replaced with 200µl of fresh medium supplemented with 1nM phenyl thiourea and Texas red-conjugated Escherichia coli K-12 particles (Molecular Probes BioParticles® E-2863) for a density of 53000 particles/mm2 and incubated for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... The standard curve was obtained using a commercial control (E. coli DH10B library control in Ion Library TaqMan™ Quantitation Kit, Thermo Fisher Scientific Inc.) diluted to 6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... The agarase-treated samples were then carefully poured into combing reservoirs containing 1.2 ml of MES solution supplemented with 2 mM Zn(O2CCH3)2 and either S1 nuclease (40 U/ml) or S1 nuclease dilution buffer (Thermo Fisher) and were incubated for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... media was changed to Hepatocyte Medium E (William’s E Medium [Gibco] ...
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bioRxiv - Genomics 2023Quote: ... the plate containing the lysed cells is supplemented with RNA spike-ins (1 μL of a 1:1,600,000 dilution of ERCC spike-in mixture A (Life Technologies)) ...
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bioRxiv - Plant Biology 2021Quote: ... and then incorporated into the destination vector pMV3 (driven by the cauliflower mosaic virus (CaMV) 35S promoter) using the LR recombination reaction (Invitrogen, USA). All the recombinant constructs were transformed into the Agrobacterium tumefaciens strain C58 by electroporation and subsequently transformed into the tomato genome via explants of cotyledon ...
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bioRxiv - Genomics 2022Quote: ... and the blocked mosaic end (Extended Data Table 1; PSS3) were obtained from IDT and resuspended in Ultrapure water (Invitrogen 10977015) to a final concentration of 100 uM (Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... The gel was then stained using Pro-Q Emerald 300 staining for glycoproteins kit (Invitrogen) following the procedure described by the supplier.
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... All liquid cultures were agitated at 250 rpm (E. coli) or 180 rpm (P. putida) in an orbital MaxQ™ 8000 shaker incubator (ThermoFisher Scientific, Waltham, MA, USA). Solid media contained 15 g L−1 agar ...
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bioRxiv - Cell Biology 2023Quote: ... Expression constructs were made by LR reaction using the following destination vectors: pDEST15 (for N-terminal GST-fusion in E. coli, cat#11802014, Thermo Fisher Scientific, Waltham, MA, USA) and pcDNA-DEST53 (for N-terminal GFP fusion in mammalian cells ...
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bioRxiv - Molecular Biology 2020Quote: ... To 1/3 of the sample 1/10 of the recommended amount of spike-in (ERCC RNA spike-in mix, Ambion) was added ...
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bioRxiv - Immunology 2021Quote: ... Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+Spike+Spike+ cells were sorted into individual wells containing RNase OUT (Invitrogen), First Strand SuperScript III buffer ...
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bioRxiv - Immunology 2023Quote: RNA was isolated using TRIfast [46] (Peqlab) with pre-isolation D.melanogaster Schneider S2 spike-in (1:10) and post-isolation ERCC spike-ins (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Viral copy number quantification by RT-qPCR was performed using Detection Kit for 2019 Novel Coronavirus (2019-nCoV) RNA (PCR-Fluorescent Probing) (Cat. #DA-930) (China) in a QuantStudio 3 real-time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin was visualized with rat anti-E-cadherin (Invitrogen #13-1900), both at 1:100 concentrations as per manufacturer protocol ...
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bioRxiv - Genomics 2020Quote: ... and A3B3 (Table S1) were synthesized (Thermo Fisher Scientific) and cloned into BamHI/XbaI restriction sites of the pcDNA3.1(+ ...
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bioRxiv - Synthetic Biology 2019Quote: ... S1 nuclease was added (0.2 units; Thermo Fisher Scientific) and samples were incubated at 37 °C for 10 minutes and quenched in a solution of 7 M Urea and 10 mM EDTA ...
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bioRxiv - Pathology 2019Quote: ... All primer sequences (Table S1) were synthesized by Invitrogen Biological Company (Shanghai ...
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bioRxiv - Cell Biology 2021Quote: S1/S2 basal media: 500 mL MCDB131 (Life Technologies) supplemented with 0.75 g NaHCO3 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... then digested with T7 Exonuclease and S1 Nuclease (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... treated with the S1 nuclease (18001-016, ThermoFisher Scientific) at 20 U/mL in S1 buffer (30 mM sodium acetate pH 4.6 ...
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bioRxiv - Genetics 2021Quote: ... ERCC ExFold RNA Spike-In mixes (Thermo Scientific) were added as internal control ...
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bioRxiv - Genomics 2020Quote: ... ERCC RNA Spike-In (ThermoFisher Cat. No. 4456740) was diluted in the lysis mix following the manufacturer’s user guide and previous studies36 ...
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bioRxiv - Microbiology 2020Quote: ... Spike RBD or NP in 1X PBS (Gibco) and incubated overnight at 4°C ...