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Citations for Thermo Fisher :
101 - 150 of 3707 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The pool was incubated with biotinylated SARS CoV2 Spike extracellular domain bound to 5 μL streptavidin M280 dynabeads (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with 2.5 µg of a SARS-CoV spike protein polyclonal antibody (PA1-41165, Thermo Fisher Scientific) for 30 min on ice before washing with 2.5 mL of FACS buffer and centrifuging at 1750 RPM for 7 min ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (kindly provided by Eric Fischer, DFCI) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with diluted plasma samples to allow antibody binding to occur ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike gene was amplified by PCR using Phusion Hot Start II DNA polymerase (Thermo Scientific™) and a set of primers recognizing sequences upstream and downstream of the spike gene (Forward ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
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bioRxiv - Cell Biology 2022Quote: ... DNA polymerases and NEBuilder HiFi DNA assembly master mix were obtained from New England Biolabs. Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (ThermoFisher Scientific). Buffers for assessing fluorescence lifetime were composed of either 0.01M PBS (pH 6.5 – 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... and Escherichia coli S15 (AP009048.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
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bioRxiv - Cell Biology 2022Quote: ... All synthetic oligonucleotides were obtained from Integrated DNA technologies (IDT). Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (ThermoFisher Scientific). mApple and TfR DNA was obtained from mApple-Lysosomes-20 (RRID:Addgene_54921 ...
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bioRxiv - Biophysics 2022Quote: ... novicida U112 (Fn)Cas12a (WP_003040289) was codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The FnCas12a gene was inserted into the 1B plasmid (Addgene #29653 ...
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bioRxiv - Neuroscience 2023Quote: ... 15 μg/mL and 50 000 cells/mL respectively. Bioparticles of pHrodoTM Green-labelled Escherichia coli (E. coli) were purchased from Thermo Fisher Scientific and used at a concentration of 25 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Sequences of F0 mosaic cuts were confirmed by TOPO cloning (Invitrogen) and sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were transformed into chemocompetent bacteria (E. coli Top10™, Thermo Fisher Scientific, TFS), which were generated like previously described70 ...
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bioRxiv - Bioengineering 2023Quote: MMP9-pro-cat-Hiswt (E coli expression) was expressed in BL21(DE3) (Thermo Fisher). Cells were grown in 2xYT at 37°C and expression was induced with 0.5 mM isopropyl ?-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Bioengineering 2021Quote: ... and 2.5 μg of SARS-CoV-2-d18 spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Microbiology 2022Quote: Hybridoma Screening: Expi293F cells were transiently transfected to express SARS-CoV-2 spike (Wuhan) using Lipofectamine 3000 (L3000001, Thermo Fisher) and then incubated with supernatant from the hybridoma cell lines from each fusion ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Immunology 2022Quote: ... transfected HEK293T cells were stained with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) and an Alexa Fluor 488-conjugated goat anti-rabbit IgG (A11034, Invitrogen). Cells were then acquired on a BD Accuri C6 plus and analyzed by FlowJo software version 10.6.2.
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bioRxiv - Immunology 2021Quote: HEK293T cells were transiently transfected with plasmids encoding full length SARS-CoV-2 spike variants using lipofectamine 3000 (L3000-001, ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...
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bioRxiv - Immunology 2020Quote: The prefusion ectodomain of the SARS-CoV-2 Spike protein was transiently transfected into suspension-adapted ExpiCHO cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Immunology 2021Quote: HEK293T cells were transiently transfected with plasmids encoding full length SARS-CoV-2 spike variants using lipofectamine 3000 (L3000-001, ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Protein labelled cells were stained with biotinylated SARS-CoV-2 S1 (ACRO biosystems – S1N-C82E8) and detected with streptavidin AF647 (Invitrogen – S21374). Cells were stained with 7-AAD viability staining solution at 5 μl/1×106 cells to determine live cells ...
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bioRxiv - Biophysics 2020Quote: The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). The plasmids were a kind gift from Prof ...
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bioRxiv - Cell Biology 2020Quote: ... vector restriction digests or DNA oligonucleotides with compatible overhangs. Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... with the resulting plasmid confirmed as correct by Sanger sequencing (Genewiz). The Escherichia coli (E. coli) DH5-α strain (Thermo Fisher Scientific) was used for plasmid amplification ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Genetics 2022Quote: ... Tissues were incubated with mouse anti-P-glycoprotein C219 antibody at 3.6μg/mL (Glycoprotein Monoclonal Antibody C219, Cat. No MA1-26528, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A glycoprotein ladder (Candy Cane ladder; Invitrogen) served as positive control ...
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bioRxiv - Microbiology 2024Quote: ... along with a CandyCane glycoprotein ladder (Thermofisher). We additionally loaded the same two B ...
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bioRxiv - Biochemistry 2022Quote: ... and transfected with 24 µg of plasmid encoding either the wildtype or E406W SARS-CoV-2 spike protein using Lipofectamine 2000 (Life Technologies). Four hours at transfection ...
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bioRxiv - Microbiology 2021Quote: A codon optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg of plasmid encoding SARS-CoV2 spike (D614) with C-terminal 18 amino acid deletion using Lipofectamine LTX Plus reagent (Invitrogen, USA) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: A codon-optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The positive control was addition of 10 μg/mL of SARS-CoV-2 spike protein antibody (pAb, T01KHuRb) (ThermoFisher, MA, USA) at the time of infection.
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2021Quote: Point mutations to generate the mutants were introduced by site-directed mutagenesis of phCMV-SARS-CoV-2-Spike using Q5 polymerase (Thermo Scientific) and validated by sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Immunology 2022Quote: ... Sections were then incubated with cocktails of primary antibodies: rabbit anti-SARS-CoV-2 Spike Protein (1:100, Invitrogen, #MA5-36087) + rat anti-ERα H222 (1:100 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 5% FBS and 0.5% Triton X-100) for 30 minutes and washed before incubation with an anti-SARS-CoV-2 spike protein antibody (Life Technologies). Nuclei and F-actin were stained using DAPI and Phalloidin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 virus was labelled using first an anti-SARS-CoV-2 antibody (Spike protein, Thermo Fischer) and then a secondary anti-rabbit Alexa 647 (Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... and the SARS-CoV-2 spike gene was reverse-transcribed and amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Immunology 2020Quote: ... The cells were blocked with 5 % skim milk-PBS for 1 hour at RT and incubated with an antibody against SARS-CoV-2 spike (BLSN-005P, Beta Lifescience) or control IgG (Thermo Fisher) at 4 °C overnight ...