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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... cDNAs and qPCR primers were combined with 2X SYBR master mix from Thermo Fisher for qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR was performed using 2 uL of cDNA and primers for 18S (ThermoFisher #Hs03003631), Pax3 (ThermoFisher #Bt04303789) ...
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bioRxiv - Cell Biology 2022Quote: ... The qPCR primers were purchased as pre-optimized reagents (Applied Biosystems/Life Technologies, Inc.) and the TaqMan One-Step RT-PCR kit was used to perform all reactions ...
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bioRxiv - Cell Biology 2022Quote: ... The qPCR primers were purchased as pre-optimized reagents (Applied Biosystems/Life Technologies, Inc.) and the TaqMan One-Step RT-PCR kit was used to perform all reactions ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was conducted using synthesized primers and the SYBR green master mix (ThermoFisher, 4309155) on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Primers were added to TaqmanTM Fast Advanced Master Mix for qPCR (Thermo Fisher, 4444554) and then combined with cDNA in a MicroAmpTM Fast Optical 96-well Reaction Plate ...
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bioRxiv - Microbiology 2024Quote: ... The oligonucleotides used as primer sequences for the qPCR experiments were purchased from ThermoFisher and are reported in Extended Data Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Relative quantification using standard curve method on the input was performed for each primer pair and 96-well Optical Reaction plates were read in a StepOnePlus PCR instrument (Applied Biosystems). Fold enrichments were calculated as fold inductions relative to the values measured with IgG ...
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bioRxiv - Genomics 2020Quote: ... that were generated from the capillary sequencing were pre-processed based on the sequence alignment of the designed primer pairs using the Vector NTI software (Invitrogen, USA). The trimmed sequences were then loaded to the CAP3 Sequence Assembly Program (Huang and Madan 1999 ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were amplified using appropriate primer pairs (Tables S2 and S3) and Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific). Linear fragments were purified on agarose gels ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 µL of the amplified product was subjected to another PCR reaction using primer pair AK4340F2 and AK4630R2 (Kapoor et al., 2013) along with the DreamTaq DNA Polymerase (ThermoFisher Scientific). The PCR conditions comprised of 3 min of denaturation at 95 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR-positive samples were amplified with the same primer pair and sequenced with the dideoxy chain-termination protocol on an ABI PRISM 3730xl Genetic Analyzer (Applied Biosystems) using the BigDye Terminator cycle sequencing kit (Perkin Elmer ...
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bioRxiv - Plant Biology 2021Quote: ... with the primer pair cRBR-GWF/ cRBR-GWR and cloned into pDONR221 vector with the Gateway BP clonase II enzyme mix (Invitrogen, 11789020), and the resulting pDONR221-RBR_N*AB*C* phospho-variants entry clones were recombined into pDEST32 by Gateway LR clonase II enzyme mix (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the plasmid complement of each isolated clone was determined using primer pairs previously described (77) and DreamTaq Green DNA Polymerase (ThermoFisher Scientific). Each clone was confirmed to contain all B31-specific plasmids ...
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bioRxiv - Microbiology 2020Quote: ... The genes of interest and pfhsp70-1LID- were PCR-amplified using specific primer pairs (SI Appendix, Table S1) and Platinum Taq DNA polymerase (ThermoFisher Scientific) (2 U/reaction) ...
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bioRxiv - Microbiology 2023Quote: ... rnpM was amplified using the primer pair DW40/DW41 and cloned between the BsaI and XhoI sites of the expression vector pETSUMOadapt (Invitrogen, Germany). The resulting plasmid was pGP3618.
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bioRxiv - Immunology 2023Quote: ... 2.5 µl of a 200 nM stock pool of each primer pair (Supplementary File 1) was added to 5 µl of TaqMan PreAmp Master Mix (Applied Biosystems) and 2.5 µl of 1:5 dilution of cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Diluted cDNA was used together with selected primer pairs and Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, #4368702) on a ViiA7 device (Applied biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... the MpROPGAP and MpREN coding sequences were amplified using PCR by KOD Plus Neo DNA polymerase (TOYOBO) with the appropriate primer pairs (Table S1) and cloned into the pENTR/D-TOPO cloning vector (Life Technologies). To generate proMpE2F:XVE>>MpROPCA ...
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bioRxiv - Plant Biology 2024Quote: ... were amplified from wild-type Tak-1 genomic DNA using PCR with the appropriate primer pairs (Table S1) and cloned into the pENTR/D-TOPO cloning vector (Life Technologies). These entry vectors were used in the Gateway LR reaction (Life Technologies ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Primer pairs were designed using NCBI Primer-BLAST41 and ApE42 to flank each SNP by ∼250 base pairs (giving approximately 500bp amplicons) and ordered from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA fragment contained in each clone was PCR amplified using gene specific primer pairs and the Platinum SuperFi II mastermix (Invitrogen™, ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting solution was used to perform multiplex PCR using primer pairs specific for each endogenous plasmid of strain B31 [61] and the DreamTaq Green DNA Polymerase (Thermo Scientific). For genomic DNA extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... standard curve analysis was carried out to ensure that the amplification efficiency of PCR for each primer pair was within the prescribed range of 90-110% (Applied Biosystems). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Triplicate reactions for each cDNA and primer pair were run on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) and transcript levels relative to GAPDH were calculated using the ΔCt method.
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bioRxiv - Microbiology 2024Quote: The VP37 and HSP90 coding sequences were PCR-amplified from the clones used in YTHA using specific primer pairs (Supplementary Table S1) and inserted into the pENTRTM1A vector (Invitrogen, USA) using the SalI and XhoI sites ...
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bioRxiv - Microbiology 2024Quote: ... PRR1 and MS2 were amplified with the primer pairs (Table 2) using Phusion™ high-fidelity DNA polymerase (Thermo Fisher Scientific). The amplified products were gel-purified and digested with BamHI and PstI (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: Enrichment of Zfp263 at specific sites in the genome was probed using custom-designed primer pairs using Sybr green dye-based detection of amplification products on the Quantstudio 7 (Thermo Fisher). The reaction condition and primer details are in the Supplementary Method Table 2 ...
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bioRxiv - Systems Biology 2024Quote: ... along with primer pairs and run in triplicates for quantitative real time PCR on a QuantStudio 5 Real-time PCR System (Applied Biosystems). Primers were designed using the NCBI/Primer Blast tool (Supplementary Table 1) ...
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bioRxiv - Bioengineering 2021Quote: ... Alpha-MEM (Gibco, Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... and 20uL was added to each well of a 96 well TaqMan Array plate with chemokine/chemokine receptor primers (ThermoFisher, Mouse Chemokines & Receptors Array plate ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then processed for real-time polymerase chain reaction (RT-PCR) to assess the expression of the oxytocin receptor (primers and probe assay ID: Rn00564446_g1, purchased from Life Technologies). In particular ...
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bioRxiv - Neuroscience 2021Quote: ... and 20 ng/mL Platelet-derived growth factor (PDGF, Invitrogen, USA) for 3 days at 37 °C under a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL each of platelet-derived growth factor (PDGF) (Gibco), and fibroblast growth factor (FGF ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from 6 pairs of P21 mouse testes (3 pairs of wild type and 3 pairs of Mov10-/-) using TRIzol reagents (Thermo Fisher Scientific). 1 μg of total RNA from each sample was used to generate RNA-seq libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat ...
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bioRxiv - Genetics 2024Quote: ... cDNAs were then used to launch the qPCR reaction using gene-specific primers along with the Platinum SYBR green qPCR SuperMix-UDG (#Cat.11733038, Invitrogen, Massachusetts, USA). Using BioRad CFX96TM Real-time System (C1000 TouchTM Thermal Cycler) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... with specific primer pairs (table 1) were used for all qPCR assays and analyzed via QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Technical and experimental triplicates were performed for all samples ...
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bioRxiv - Bioengineering 2020Quote: ... Fifty microliter clots were formed from purified fibrinogen using 0.25 U/mL human alpha-thrombin (Fisher Scientific), 2 mg/mL fibrinogen (Enzyme Research Laboratories) ...
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bioRxiv - Biochemistry 2020Quote: Specific primers and probes for real-time qPCR analysis of Atlantic haddock were designed with Primer Express software (Applied Biosystems, Carlsbad, California, USA) (cyp1a and the technical reference ef1a ...
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bioRxiv - Physiology 2020Quote: All forward and reverse quantitative PCR (qPCR) primers (Table 1) were designed using Primer Express 3.0.1 (Applied Biosystems, ThermoFisher Scientific, Wilmington, DE, USA). Primers were designed using sequences from the brook trout transcriptome from Sutherland et al ...
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bioRxiv - Microbiology 2022Quote: ... 500 ng of cDNA were amplified by 40 PCR cycles using an inverse primer pair SuperFi II DNA polymerase (Thermo Fisher Scientific). The linear RNAs were amplified using 100 ng of cDNA for 25 cycles of PCR amplification using a corresponding primer pair ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR reactions were carried out with the indicated primer pairs (Eurofins MWG Operon, 500 nM final concentration, Supplementary Table 5) and Power SYBR® Green PCR Master Mix (Applied Biosystems) and read in MicroAmp® Fast Optical 96-well plates (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR reactions were carried out with the indicated exonic primer pairs (Eurofins MWG Operon, 500 nM final concentration, Table 3) and the Power SYBR® Green PCR Master Mix (Applied Biosystems) and read in MicroAmp® Fast Optical 96-well plates (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... H2AK115R/K116R and H2AK119R were amplified by two pairs of primers (LH3848/LH4309 and LH3848/LH3849) and sub-cloned into the pENTR-TOPO vector (Thermo Fisher Scientific) to generate pENTR-H2AK115R/K116R and pENTR-H2AK119R ...
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bioRxiv - Cell Biology 2020Quote: ... Duplex reaction format was utilized with FAM-labeled probe/primer pairs for the gene of interest and VIC-labeled probe/primer-limited pairs for TBP (Tata-Binding Protein) as the normalizer gene (ThermoFisher, Waltham, MA). For real-time PCR reactions ...
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bioRxiv - Immunology 2023Quote: ... in order to conduct real-time PCR using the indicated primer pairs from PrimerBank (24) (Table S1) with SYBR Green master mix (ThermoFisher, Waltham MA).
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bioRxiv - Cell Biology 2024Quote: ... to determine the transcript expression of target genes using the respective primer pair and SYBR green master mix (#A25741, Thermo Scientific, US). Gapdh was used as the housekeeping gene for normalization ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA fragment contained in each clone was PCR amplified using gene specific primer pairs and the Platinum SuperFi II mastermix (Invitrogen™, ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... primer pairs targeting wildtype with or without cryptic sequences (see table below) and Phusion Plus Green PCR Master Mix (Thermo Scientific, F632L). Upon cryptic exon inclusion ...