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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Transferrin receptor (Invitrogen, 13-6890), NHE5 (PA5-37222) ...
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bioRxiv - Cancer Biology 2021Quote: ... FAM-labeled target primer and endogenous human ACTB control (beta actin) (Thermo Fisher Scientific) were mixed with master mix and template ...
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bioRxiv - Cell Biology 2022Quote: ... TaqMan RT primers against human occludin (Hs05465837_g1) and GAPDH (Hs02786624_g1) were purchased from Invitrogen.
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bioRxiv - Developmental Biology 2024Quote: ... For the expression analysis of globins in human cells predesigned TaqMan primers (Applied Biosystems) were used in conjunction with Taqman Universal Master Mix II (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR for fish samples were performed by primers in combination with Power SYBR Green (Thermo Fisher Scientific) using a ViiA7 real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and qPCR was performed using custom primers (Table S2) and PowerUp SYBR Green 2X Master Mix (ThermoFisher) on QuantStudio6 or 12k Real-Time PCR instruments (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed with the primers in Supplementary Table 1 using the POWRUP SYBR Master Mix (ThermoFisher). qPCR was performed on the QuantStudio6 real-time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... Primers obtained from Elim Biopharmaceuticals were run with SYBR™ Green qPCR Master Mix (Thermo Fisher Scientific) on an ABI PRISM 7900 Sequence Detection System to quantify mRNA transcript levels ...
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bioRxiv - Microbiology 2023Quote: ... primer efficiencies were determined by performing a qPCR with SYBR green (Applied Biosystems, Waltham, MA, United States) and a Applied Biosystems 7300 Real-Time PCR System using the manufacturer’s protocol with at least 4 concentrations of a 10-fold dilution series of gDNA and the primers listed in table 2 at 400 nM concentration ...
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bioRxiv - Bioengineering 2022Quote: ... Quantitative PCR was performed using PrimeTime qPCR Primers (Integrated DNA Technologies) and PowerUp SYBR Green (Thermo Fisher) on a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... RNA was collected for qPCR using a commercially available Taqman primer-probe for SCAF1 (ThermoFisher Scientific, Hs01553675_m1). 250 (TP53−/− ...
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bioRxiv - Bioengineering 2024Quote: ... Quantitative PCR was performed using PrimeTime qPCR Primers (Integrated DNA Technologies) and PowerUp SYBR Green (Thermo Fisher) on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... and qPCR was performed by Taqman primers with TaqMan™ Fast Advanced Master Mix (Applied Biosystems™) on StepOnePlus Real-Time PCR Systems (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... alpha-MEM (Thermo Fisher Scientific) was supplemented with 2.5 % FBS ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with the primary antibody solution (1:100 dilution; PDGFRA-APC, Thermo Fisher, cat#17-1401-81 ...
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bioRxiv - Developmental Biology 2022Quote: ... We used the following antibodies: PE anti-mouse CD140a (PDGFRA) (Thermo Fisher Scientific, 12-1401-81), APC anti-mouse CD31 (PECAM-1 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were incubated overnight at room temperature with Goat anti-PDGFRA (Invitrogen, CAT#, 1:200), HuC/HuD (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... step of 14 cycles using dilute (50 nM in a 5-uL reaction) primer pairs of each assay with TaqMan Preamp MasterMix (Applied Biosystems, Foster City, CA, USA) was performed according to the BioMark protocol (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... Specific primer pairs (Supplementary Table S4) were used in each qRT-PCR with the SYBR Green PCR Master Mix Kit (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 1 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... TRAC knockouts were detected via staining with anti-human alpha/beta TCR FITC (Thermo Fisher Scientific, catalog #11-9955-42). Anti-CD19 CAR expression was detected using FITC-labelled human CD19 protein (Acro Biosystems ...
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bioRxiv - Bioengineering 2022Quote: ... The serum-free medium used for encapsulation was alpha minimum essential medium (alpha-MEM, Gibco) supplemented with 10 % bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... sorted cells were cultured on irradiated OP9DLL1 monolayers in Alpha-MEM (Alpha-MEM, ThermoFisher, 12000063) supplemented with 20% FBS (HyClone) ...
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bioRxiv - Cancer Biology 2024Quote: ... we determined the human origin of each PDX tumor by quantifying the ratio of human-to-mouse gDNA in PDX tumors via qPCR with a human RNaseP gene probe (20x human RNaseP copy number assay, FAM-TAMRA, Life Technologies) and a mouse Trfc gene probe (20x mouse Trfc copy number assay ...
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bioRxiv - Bioengineering 2020Quote: ... and biotinylated detection antibody pairs from Invitrogen™ ...
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bioRxiv - Microbiology 2021Quote: ... 5µl were subjected to quantitative PCR using primer/probe sets for human Gapdh (Applied Biosystems), and EBOV trVLP 5’-trailer region ...
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bioRxiv - Neuroscience 2022Quote: Random primers were used to construct a cDNA library from human foetal brain mRNA (Invitrogen) into the pP6 plasmid derived from the original pACT2 ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR was performed using let-7 and 2S rRNA TaqMan MicroRNA Assay primer mix (Thermo Fisher Scientific) with the TaqMan Small RNA Assay (Applied Biosystems).
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bioRxiv - Cell Biology 2020Quote: ... Gene-specific primers were used to perform qPCR on the ABI 7500 Real-Time PCR System (Applied Biosystems) with MESA GREEN qPCR MasterMix Plus and SYBR® Assay Low ROX (Eurogentec ...
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bioRxiv - Bioengineering 2021Quote: Primer efficiency was determined by preparing qPCR reactions with PowerUp SYBR® Green Master Mix (Life Technologies™), with a 5x dilution series of cDNA (500ng/μL - 0.0061ng/μL) ...
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bioRxiv - Neuroscience 2021Quote: ... Primers are reported in Table 3 and qPCR was performed with SYBR green PCR master mix (Applied Biosystems). Samples were excluded if technical replicates failed ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was carried out with TaqMan® probe/primers (Applied Biosystems; see Table S3 for Unique assay IDs). Beta-actin (ACTB) ...
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bioRxiv - Cell Biology 2024Quote: Primers for qPCR (Table 1) were confirmed using standard PCR using DreamTaq master mix (Thermo Fisher, cat: K1082), 500 ng cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... with 10 ng of cDNA and 250 nM final primer concentration on a QuantStudio3 qPCR machine (Applied Biosystems). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was carried out using sequence-specific primers and PowerTrack SYBR green master mix (ThermoFisher, catalog number: A46109). Relative mRNA expression was calculated by the 2−ΔΔCT method of quantitative PCR after normalization with 18S rRNA levels ...
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bioRxiv - Neuroscience 2024Quote: ... The expression of specific mRNAs was determined by RT-qPCR using gene-specific SYBR Green-based primers (Invitrogen). Each individual RT-qPCR assay was carried out in triplicates ...
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bioRxiv - Cell Biology 2021Quote: The expression cassette consisting of a Kozak sequence followed by the coding sequence for human diphtheria toxin receptor (hDTR; UniProt Q53H93) was codon-usage optimized for expression in mice and synthesized (Thermo Fisher Scientific/GeneArt ...
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bioRxiv - Cell Biology 2021Quote: ... gene expression was analysed using the TaqMan™ Array Human Cardiomyocyte Differentiation by BMP Receptors (Thermo Fisher Scientific, Waltham, MA). Methods are detailed in the online Supplementary material.
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bioRxiv - Molecular Biology 2020Quote: ... and Transferrin Receptor (1:1,000; Invitrogen). Proteins were detected with HRP-conjugated secondary antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... anti‐transferrin receptor (1:500; Invitrogen), anti‐α‐fodrin (1:5000 ...
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bioRxiv - Immunology 2024Quote: ... followed by Fc receptor blocking (Invitrogen) for 10 minutes and surface staining for 30 minutes with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight in rat anti-CD140a (PDGFRA) (ThermoFisher; #14-1401-82; RRID: AB_467491; 1:50) and rabbit anti-TRPV1 (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: Human skin fibroblast cell line GM07492 (Coriell Cell Repository) was grown in MEM Alpha medium (Life Technologies, catalog number 12561-056) with 10% FBS (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... alpha-pinene (Acros Organics, CAT#: 131270050); limonene (Tokyo Chemical Industry ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-Tubulin (MS581P1) from Thermo Fisher, SEC23B (GTX82432 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:3000 alpha-bungarotoxin 488 (Invitrogen), 1:1000 rabbit anti-Syntaxin1 (gift of Thomas Südhof ...
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bioRxiv - Immunology 2020Quote: ... and Interferon alpha (Invitrogen, Cat. BMS6027TWO). Plates were read using an Epoch BioTek Gen5 microplate reader at 450nm ...
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bioRxiv - Physiology 2024Quote: ... and alpha-smooth muscle actin (ThermoFisher Scientific ...