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Citations for Thermo Fisher :
151 - 200 of 5001 citations for Fab pHast human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The Fab fragments of Clone 2 and Clone 6 were generated from full length IgGs of Clone 2 and Clone 6 using a commercial PierceTM Fab Preparation Kit (Thermo Fisher). All procedures were performed following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Cell Biology 2021Quote: ... TOMM22 was revealed with a species-specific Fab’ secondary antibody coupled to Alexa 647 (Thermo Fisher Scientific, A-21237 or A-21246). TOMM20 and PMPCB were revealed with either secondary antibody depending on the co-stained protein ...
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bioRxiv - Immunology 2022Quote: ... and anti- TCRα (clone H28-710 or H28) mAbs were obtained by digesting commercial full-length mAbs using Fab preparation kit (Thermo Fisher 44685). H57 scFv was a generous gift from Johannes Huppa of the Medical University of Vienna (10).
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bioRxiv - Physiology 2021Quote: ... Alexa Fluor 546-conjugated goat anti-rabbit IgG (Fab)2 fragment and Alexa Fluor 488 conjugated goat anti-mouse IgG were from Invitrogen (Taastrup, Denmark). All primary antibodies were diluted in PBS with 5% normal goat serum ...
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bioRxiv - Bioengineering 2020Quote: ... Pro + using an optimized autoinduction media and purified by protein A affinity chromatography similarly to Fabs.30 VH-Fc were expressed in Expi293 BirA cells using transient transfection (Expifectamine, Thermo Fisher Scientific). 4 days after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Fabs were generated by Papain cleavage and purified by the use of the Pierce Fab Preparation Kit according to the manufacturers’ instructions (Thermo Scientific - #44985). The purity of the Fabs was confirmed by SDS-PAGE.
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bioRxiv - Biophysics 2022Quote: ... The supernatant containing the secreted Fab was isolated by centrifugation (4000xg for 20 minutes) and applied to 1-2mL of IgG-CH1 affinity resin (Thermo Fisher Scientific) pre-equilibrated with 1xHBS ...
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bioRxiv - Biophysics 2022Quote: ... Suitable grids and grid regions of hSERINC3-Fab samples were identified by screening atlas images on the UVa MEMC Titan Krios (Thermo Fisher Scientific) using the Falcon 3EC detector ...
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bioRxiv - Cell Biology 2023Quote: ... the disulfide bond linking the two Fab fragments of F(ab’)2 goat anti-mouse Ig(G+M) was reduced using 2-mercaptoethylamine HCL (Thermo Fisher Scientific) and biotinylated by using maleimide-PEG2-biotin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: The sandwich ELISA assay was performed with 4 µg/ml coated F(ab’)2 fragments that were generated from full-size antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher, 44980) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Immunology 2023Quote: Fabs used for ns-EM were purified as follows: expressed IgGs were concentrated and digested using the Pierce™ Fab Preparation Kit (Thermo Fisher 44985) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Human transferrin-568 (ThermoFisher) was added at 10μg mL−1 concentration in serum free media and incubated at 37°C to allow for internalization ...
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bioRxiv - Immunology 2021Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: Probes Hs00171064_m1 (human, ThermoFisher), Mm00440280_g1 (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human hepatocytes (Gibco) were seeded in the top channel at a density of 3.5 x 106 cells/mL using complete hepatocyte seeding media ...
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bioRxiv - Immunology 2020Quote: ... or anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 25 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... or human IgG (Invitrogen) as control ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (Gibco) at 50 μg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... human (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (ThermoFisher, #33016015); Laminin 111 ...
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bioRxiv - Immunology 2023Quote: ... A Human ProcartaPlexTM (Invitrogen) immunoassay was additionally used to detect 45 human cytokines ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... CD36 (Human tissue Thermofisher: PA1-16813 1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... VDAC1 or COX2 were revealed by a species-specific Fab’ secondary antibody coupled to Alexa 555 (Thermo Fisher Scientific, A-21430 or A-21425). TOMM22 was revealed with a species-specific Fab’ secondary antibody coupled to Alexa 647 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescently labeled Fabs were made by conjugating with either Alexa Fluor 488 or Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific, A20000 or A20006) to lysine side chains in PBS and molar ratio of dye to Fab was controlled to be in the range of 1 ∼ 2.
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bioRxiv - Immunology 2022Quote: ... M8a-34 Fab or HSW-1 Fab and SARS-CoV-2 Omicron BA.1 spike 6P with M8a-31 Fab were collected using SerialEM automated data collection software (Mastronarde, 2005) on a 300 keV Titan Krios (Thermo Fisher Scientific, Waltham, MA) cryo-electron microscope equipped with a K3 direct electron detector camera (Gatan ...
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bioRxiv - Immunology 2023Quote: ... The fragment antigen-binding (Fab) part was separated from undigested IgG and the crystallizable fragment (Fc) using an Immobilized Protein A column (Thermo Fisher Scientific, No. 20356). The flow through containing the Fab was concentrated through 10 kDa Amicon centrifugal filter units (EMD Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Primary human vein endothelial cells (HUVECs) were cultured in human endothelial SFM medium (Invitrogen) containing 20% foetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) and cultured in AIMV medium (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... + 2% human serum albumin (ThermoFisher) + 5 μM ROCKi (Tocris) ...
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bioRxiv - Cell Biology 2020Quote: ... Human GR-specific siRNA (Invitrogen) was used at a concentration of 100 nM for 48 h to effectively knock down GR ...