Labshake search
Citations for Thermo Fisher :
101 - 150 of 5001 citations for Fab pHast human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... The Fab was then purified using a CaptureSelect CH1-XL pre-packed column (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2023Quote: 7G5 Fab [16] were expressed in transiently transfected Expi-293F cells (Thermo Fisher Scientific, Waltham, MA) and purified by affinity chromatography using protein A/G agrose (Beyotime ...
-
bioRxiv - Immunology 2021Quote: The RAM2 Fabs were generated by papain digestion and purified using Immobilized papain agarose resin (Thermofisher Scientific) following manufacturer’s protocol ...
-
bioRxiv - Biophysics 2022Quote: ... The Fab yield was quantified by measuring absorbance at 280 nm on a Nanodrop One (Thermo Scientific) and evaluated by SDS-PAGE under reducing and non-reducing conditions.
-
bioRxiv - Immunology 2020Quote: Purified monoclonal antibodies (Fab/IgG) were buffer exchanged into PBS using equilibrated ZebaSpin columns (Thermo Fisher Scientific). The protein concentration was determined and the antibodies biotinylated by incubating for 30 min at room temperature with EZ-Link NHS-PEG4-Biotinylation reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... the Fab was buffer exchanged into Tris buffer by centrifugation with a Zeba spin column (Thermo Scientific).
-
bioRxiv - Microbiology 2020Quote: ... stained with custom Fab b12-Atto565 (25 nM) and Transferrin-AlexaFluor647 (Transferrin-AF647; Invitrogen; 25 μg/mL) in media with 6% BSA ...
-
bioRxiv - Biophysics 2021Quote: ... Primary antibodies were detected using Fab fragments coupled to Alexa Fluor 647 (Thermo Fisher, dilution 1:500) or secondary antibodies coupled to Cy5.5 (Jackson Immunoresearch ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated with spectrally appropriate Alexa Fluor-conjugated Fab fragments against primary species antibodies (Molecular Probes) for 1h ...
-
bioRxiv - Immunology 2021Quote: ... The recombinant Fab was purified from culture supernatant using an anti-CH1 CaptureSelect column (Thermo Fisher Scientific). For the RBD/COV2-2196 complex ...
-
bioRxiv - Microbiology 2022Quote: ... The IgGs and Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
-
bioRxiv - Immunology 2023Quote: ... The Fab domain was expressed in Expi293F cells and purified by IgG-CH1 Affinity Matrix (Thermo Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The flow-through was collected and Fabs were purified using CaptureSelectTM IgG-CH1 affinity resin (ThermoFisher Scientific). 200 mM acetic acid (pH 3.5) ...
-
bioRxiv - Immunology 2021Quote: ... The IgG antibodies and Fabs were purified with a CaptureSelect™ CH1-XL Matrix column (Thermo Fisher Scientific) for affinity purification and a HiLoad Superdex 200 pg column (Cytiva ...
-
bioRxiv - Molecular Biology 2022Quote: ... movies of the hZnT7 with Fab#1 were collected on a Titan Krios G3i TEM (Thermo Fisher Scientific) operated at 300 kV ...
-
bioRxiv - Microbiology 2022Quote: ... The Fab heavy and light chains of AI774 and AI878 were individually cloned into a pcDNA3 vector (Invitrogen). A 6x-His tag was added to the C-terminus of each Fab heavy chain sequence ...
-
bioRxiv - Biophysics 2021Quote: ... samples were incubated with secondary antibody (anti-mouse AlexaFluor 647 Fab Fragment, Thermo Fisher, A21237, 1:500 dilution) for 1.5 hours at room temperature and further washed ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mouse antiHuman IgG Fab Secondary Antibody-PE and LAMP1 Monoclonal Antibody (LY1C6) eFluor 660 (all from Thermofisher Scientific); CellTiter 96® AQueous One Solution Assay (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... The IgG antibodies and Fabs were purified with a CaptureSelect™ CH1-XL Matrix column (Thermo Fisher Scientific) for affinity purification and a HiLoad Superdex 200 pg column (Cytiva ...
-
bioRxiv - Immunology 2020Quote: ... F(ab)-fragments were prepared from the corresponding antibodies using the Pierce Fab Micro Preparation Kit (Thermo Fisher) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... the Fab was buffer-exchanged into Tris buffer by centrifugation with a Zeba spin column (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2022Quote: ... The mixture was then transferred to a Protein A column to purify the Fab fragments (ThermoFisher, Catalog (Cat.) No.) ...
-
bioRxiv - Microbiology 2024Quote: All antibodies in IgG and Fab forms in this study were transiently expressed in ExpiCHO cells (Thermo Fisher). For IgGs and Fabs ...
-
bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
-
bioRxiv - Microbiology 2020Quote: Polyclonal anti-CD55 antibody and control IgG were digested using the Pierce™ Fab preparation kit (Thermo Fisher Scientific). The resulting Fab fragments were quantified on a spectrophotometer ...
-
bioRxiv - Immunology 2022Quote: ... The antigen binding fragment (Fab) portion of LY-CoV1404 was generated by proteolytic digestion using immobilized papain (ThermoFisher Scientific), followed by removal of un-cleaved protein using standard chromatography techniques ...
-
bioRxiv - Immunology 2022Quote: Intact masses of the eluted IgA1 Fabs were retrieved from the generated RAW files using BioPharmaFinder 3.2 (Thermo Scientific). Deconvolution was performed using the ReSpect algorithm between 5 and 57 min using 0.1 min sliding windows with a 25% offset and a merge tolerance of 30 ppm ...
-
bioRxiv - Molecular Biology 2021Quote: ... 012-22384 Anti DYKDDDDK mouse IgG2b) using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher). First ...
-
bioRxiv - Immunology 2023Quote: ... Prior to experiments both Fab and 6P spike were buffer-exchanged into HEPES-buffered saline (HBS, pH 7.4) using Zeba spin columns (ThermoFisher).
-
bioRxiv - Molecular Biology 2023Quote: ... The RRtoGG αEβ7 ectodomain was diluted 1:10 in non-activating buffer and incubated with digested LF61 Fab (Invitrogen) at a 1:4 molar ratio for 45 minutes at room temperature with end-over-end mixing ...
-
bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits, mouse IgG2a for anti-EGFR 199.12 quantitation [ThermoFisher Z25108] ...
-
bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
-
bioRxiv - Biochemistry 2021Quote: The Fab fragment of the ch14D5 antibody was prepared from the antibody using Immobilized Papain-agarose resin (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2021Quote: The reaction products of anti-c-Myc Fab labelled with the CF 568 dye were purified using Size exclusion chromatography (SEC-1 MAbPac, Thermofisher) (Supplementary Fig ...
-
bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
-
bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... IBL America) overnight and subsequently with goat anti-rabbit Alexa Fluor-647 conjugate IgG-Fab (1:500, Thermo Fisher Scientific) for 45 min in PHEM buffer supplemented with 1% (w/v ...
-
bioRxiv - Immunology 2019Quote: ... Fabs and IgGs were produced by transient transfection in suspension 293F or adherent HEK 293T cells using Lipofecatamine 2000 (Invitrogen) or polyethylenamine (PEI) ...
-
bioRxiv - Microbiology 2022Quote: ... EH3 and EH8 Fab fragments were generated by overnight papain digestion at 37°C using immobilized papain agarose (ThermoFisher Scientific). Fab were separated from Fc and uncleaved IgG by passage over protein A resin followed by size-exclusion chromatography on a Superose 6 10/300 column before being used in SPR binding and X-ray crystallography ...
-
bioRxiv - Immunology 2022Quote: The retention times and masses of each of the Fab molecules were retrieved from the generated RAW files using BioPharmaFinder 3.2 (Thermo Scientific). Deconvolution was performed using the ReSpect algorithm between 5 and 57 minutes using 0.1 min sliding windows with 25% offset and a merge tolerance of 30 ppm ...
-
bioRxiv - Immunology 2022Quote: The Fab fragments of anti-Muc1 mAbs 14A and 16A were expressed by Bac-to-Bac baculovirus expression system (Invitrogen). The heavy-chain (Hc ...
-
bioRxiv - Molecular Biology 2023Quote: The Fab from the CD4i antibody 17b37 was expressed by transient transfection using expression vectors encoding the LC and a C-terminally tagged HC portion of the Fab using the Expi293 expression system (ThermoFisher)7 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fabs were generated from an overnight papain digestion of IgG at 37°C using immobilized papain agarose (Thermo Fisher Scientific). The resulting Fab was separated from Fc and undigested IgG by passage over protein A resin ...
-
bioRxiv - Cell Biology 2023Quote: ... The primary antibody was detected by a Fab fragment coupled to Alexa Fluor 647 (Thermo Fisher, A21246; dilution 1:500). The sample was then covered in imaging buffer (27 ...
-
bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
-
bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
-
bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
-
bioRxiv - Immunology 2020Quote: ... the 5D5Δg Fab was produced by site-directed mutagenesis of the mAb 5D5 VH region using Accuprime Pfx Supermix (Thermo Fisher Scientific). 5D5Δg Fab was expressed in HEK293F cells and purified by chromatography as described above.
-
bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).