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Citations for Thermo Fisher :
51 - 100 of 5001 citations for Fab pHast human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Purified Hec1 Fab was then directly conjugated with Alexa 647 (Invitrogen) according to the following procedure ...
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bioRxiv - Immunology 2020Quote: ... The RM19R Fab was purified using CaptureSelect™ CH1-XL (ThermoFisher) affinity chromatography.
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bioRxiv - Cell Biology 2023Quote: ... Fabs were conjugated with Alexa Fluor 488 (Sulfodichlorophenol Ester; ThermoFisher Scientific) or Cy5 (N-hydroxysuccinimide ester monoreactive dye ...
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bioRxiv - Immunology 2023Quote: ... Fabs were purified using CaptureSelect IgG-CH1 Affinity Matrix (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... Fab fragments were purified by anti-CH1 resin (Thermo Fisher Scientific). scFv fragments for MAD8-151 ...
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bioRxiv - Immunology 2019Quote: ... and CD19 were prepared with Pierce Fab Micro preparation kit (Thermo Scientific) using immobilized papain according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: The Fab were obtained by enzymatic digestion with Ficin (Immobilized Ficin, Thermofisher) or Papain (Immobilized Papain ...
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bioRxiv - Biochemistry 2022Quote: ... Fabs were expressed by transient transfection using the Expi293 expression system (ThermoFisher). Fab expression vectors contained genes of LC and the C-terminally 6x-His tagged HC ...
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bioRxiv - Immunology 2020Quote: ... dialyzed against PBS and Fab protein captured using Talon Resin (Thermo Scientific). Fab protein was eluted with 150 mM imidazole in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... were prepared with Pierce™ Fab Preparation Kit (#44985, Thermo Scientific, USA) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... before Fab was purified with CaptureSelect™ IgG-CH1 Affinity Matrix (ThermoFisher). Bound Fab was eluted in a buffer containing 100 mM glycine pH 3.0 ...
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bioRxiv - Biophysics 2021Quote: ... The labeled fAb antibody was prepared by conjugating an Alexa647 NHS ester (ThermoFisher) to an unconjugated fAb (Goat Anti-Mouse IgM ...
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bioRxiv - Neuroscience 2019Quote: ... Fluorophore-conjugated secondary antibodies were either H&L or Fab (Thermo Fisher Scientific) at a consistent concentration of 1:400 in the following species and colors ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorophore-conjugated secondary antibodies were either H&L or Fab (Thermo Fisher Scientific) at a consistent concentration of 1:400 in the following species and colors ...
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bioRxiv - Immunology 2020Quote: ... papain digestion was performed using the Pierce Fab preparation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Antibody digestion was performed using a Fab preparation kit (Thermo Fisher Scientific, USA) and the resulting Fab fragments were analyzed using SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: Ab1485 and 8ANC195 Fabs were recombinantly expressed by transiently transfecting Expi293F cells (Invitrogen) with vectors encoding antibody light chain and C-terminal hexahistidine-tagged heavy chain genes ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits ...
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bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
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bioRxiv - Microbiology 2020Quote: ... CR3022 Fab and CR3022 IgG were transiently expressed in Expi293™ (ThermoFisher Scientific, UK) and proteins were purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Fabs were expressed in ExpiCHO cells and purified using CaptureSelect CH1-XL resin (ThermoFisher) and followed by size exclusion chromatography ...
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bioRxiv - Molecular Biology 2021Quote: ... α-FLAG antibody were digested into Fab in a Zeba Desalt Spin Column (ThermoFisher) containing immobilized Ficin undergoing gentle rotation for 3-5 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and H5.31 and H5.28 Fabs were purified with CaptureSelect IgG-CH1 (Thermo Fisher Scientific). To obtain complexes of Fabs and HA head domain ...
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bioRxiv - Biochemistry 2022Quote: ... following standard protocols supplied in the Pierce Mouse IgG1 Fab Preparation Kit (Thermo Fisher). Complexes of refolded MAGEA4 pMHC bound to 2M2 Fab were isolated by mixing the two components and separating the complex by SEC using a Superdex 200 Increase column ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher) containing immobilized Ficin while gently rotating for 3–5 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were detected with Fab fragments coupled to Alexa Fluor 647 (Thermo Fisher) or antibodies labeled with Cy5.5 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
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bioRxiv - Microbiology 2020Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
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bioRxiv - Bioengineering 2021Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography ...
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bioRxiv - Immunology 2022Quote: ... and CD19.CAR-T cells were labeled with biotinylated anti-Fab antibodies (ThermoFisher Scientific, 31803). The co-culture was then mixed with CELLection Biotin Binder (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
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bioRxiv - Cell Biology 2021Quote: ... full length rMAb-Hec1ms was first digested using a Pierce Fab Preperation Kit (Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa 532 conjugated goat α-rabbit Fab (Invitrogen, Thermo Fisher, Darmstadt, Germany,1:500 each).
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bioRxiv - Immunology 2021Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography on a HiLoad Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... the Fab fragments were collected and dialyzed into Phosphate Buffered Saline (PBS) (ThermoFisher, catalog #10010023).
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bioRxiv - Microbiology 2020Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
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bioRxiv - Immunology 2022Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific), followed by size exclusion chromatography.
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bioRxiv - Immunology 2023Quote: ... The Fab was purified with a CaptureSelect CH1-XL Pre-packed Column (Thermo Fisher Scientific), followed by a buffer exchange to Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... The Fab was purified with a CaptureSelect CH1-XL Pre-packed Column (Thermo Fisher Scientific) followed by size exclusion chromatography ...
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bioRxiv - Molecular Biology 2023Quote: A fragment encoding the anti-Fab nanobody (29) was synthesized by GeneArt (Thermo Fisher Scientific) and subsequently cloned into the pET28a vector downstream of a 6xHis tag ...
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bioRxiv - Biophysics 2021Quote: ... were functionalized via an amine coupling reaction with 20 μg of anti-digoxigenin Fab fragments (Invitrogen) per mg of beads according to a protocol described elsewhere.[67] Of note ...
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bioRxiv - Immunology 2022Quote: RBD and Fab fragment from NCV2SG48 with 6xHis-tag expressed in Expi293F cells (Thermo Fisher Scientific) were purified using Ni-NTA Agarose resin (QIAGEN) ...
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bioRxiv - Microbiology 2021Quote: ... Fab was generated by overnight papain digestion at 37°C using immobilized papain agarose (Thermo Fisher). Fab was separated from Fc and uncleaved IgG by passage over protein A resin followed by size-exclusion chromatography on a Superose 6 10/300 column before being used in SPR binding ...
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bioRxiv - Microbiology 2020Quote: ... The Fab was purified with a CaptureSelect™ CH1-XL Pre-packed Column (Thermo Fisher Scientific) followed by size exclusion chromatography ...
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bioRxiv - Molecular Biology 2022Quote: ... Fab was purified from clarified culture supernatants using a CaptureSelect CH1-XL column (Thermo Fisher Scientific) and buffer-exchanged into Tris-buffered saline (100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibody was digested into Fab using papain resin and purified by protein A beads (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The Fab was purified with a CaptureSelect™ CH1-XL Pre-packed Column (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... and the Fab was purified using a CaptureSelect CH1-XL Pre-packed Column (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... the Fabs were purified from the supernatant using Capture Select IgG-CH1 Affinity beads (Life Technologies) according to the manufacturer indications.