Labshake search
Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Total RNA from MG stimulated MCECs was extracted using peqGOLD MicroSpin Total RNA kit (Peqlab) and then converted into cDNA with a high capacity cDNA reverse transcription kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was quantified with the high sensitivity double stranded (ds) DNA Assay Kit (Qubit dsDNA HS Assay Kit, Thermo Fisher Scientific, #Q32854) on the Qubit 2.0 fluorometer.
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bioRxiv - Cell Biology 2022Quote: ... templated and sequenced using either Ion 550™ Kit-Chef and Ion 550 Chip Kit as one 16-plex library pool or Ion 540™ Kit-Chef and Ion 540 Chip Kit as one 8-plex library pool (Thermo Fisher). Two independent sequencing analyses were performed on a panel of eight samples ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplicons of these four primer pairs were then purified from 1% agarose gel using Qiagen Gel Extraction kit and concentrations were measured with Qbit High Sensitivity DNA kit (Thermo Fisher Scientific). Each amplicon was then diluted to 1×109 molecules per µl ...
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bioRxiv - Immunology 2019Quote: ... SHIV RNA was visualized with the 1-Plex ViewRNATM ISH Tissue Assay Kit using SIVmac239 or Beta actin (positive control) probe sets and ViewRNA Chromogenic Signal Amplification Kit (ThermoFisher, Waltham, MA). These two sequential slides were indivudually imaged with a Zeiss AxioObserver microscope and AxioCam MRm camera ...
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bioRxiv - Microbiology 2019Quote: ... 0.1 ml of MTT solution (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) was added to the wells following the recommendation of the commercial kit (Vybrant® MTT Cell proliferation assay kit, ThermoFisher Scientific). After 2h of incubation at 37°C ...
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bioRxiv - Pathology 2019Quote: Total RNAs were obtained from whole kidneys or cells using NucleoSpin® RNA Kit (Macherey Nagel) and reverse transcribed using High-Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific). Quantitative PCR was performed with iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... total pancreas RNA was isolated using a Qiagen RNeasy Plus Isolation Kit followed by cDNA synthesis (High Capacity cDNA RT kit, Applied Biosystems, 4374966). RT-PCR was performed using TaqMan Universal Master Mix II (Fisher ...
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bioRxiv - Microbiology 2020Quote: ... The indexed amplicons were purified using the AMPure XP kit according to the manufacturer’s instructions followed by library quantification using a Qubit™ dsDNA BR Assay Kit (Thermo Fisher Scientific). All samples were normalized to 9 nM and pooled with 5 μl of each sample.
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bioRxiv - Pathology 2020Quote: ... The pellet was rinsed twice in DDW and then lysis buffer from the Prepfiler BTA kit (PrepFiler® Express BTA Forensic Extraction Kit (Applied Biosystems) was added together with DTT (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: The transcription start site of AhLEC1A gene was identified by 5’ RACE (rapid amplification of cDNA ends) using a 5’ RACE kit (Invitrogen GeneRacerTM Kit) following the instructions provided by the manufacturer ...
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bioRxiv - Genetics 2020Quote: ... Barcoded products were pooled and gel purified from a 1% agarose gel using a QIAgen kit (#28115) to remove primers before quantitation with a Qubit dsDNA HS Kit (ThermoFisher Scientific #Q32851). Samples were loaded onto an Illumina MiSeq instrument with a 300 cycle v2 kit for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified using the QIAquick Gel Extraction Kit after electrophoresis in agarose gel 1% and then cloned with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) into competent E ...
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bioRxiv - Immunology 2021Quote: cDNA was reverse-transcribed from template RNA either using a two-step reverse transcription using AppScript cDNA synthesis kit (Appleton Woods) or using the High Capacity RNA-to-cDNA kit (Applied Biosystems, USA).
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bioRxiv - Genetics 2020Quote: ... genomic DNA was extracted using the Qiagen DNeasy Blood & Tissue Kit (Cat.D No. 69504) and quantified using the Qubit dsDNA HS Assay Kit (Invitrogen, Waltham, MA, USA). DNA purity was verified (OD260/280 > 1.8 ...
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bioRxiv - Physiology 2020Quote: ... total cholesterol E and non-esterified fatty acid (NEFA) were measured with commercially available kits following manufacturers’ instructions: Infinity TG kit (Thermo Fisher Scientific), total cholesterol E kit (Wako Diagnostics) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA concentrations were measured fluorometrically (Qubit® 3.0, kit Qubit® dsDNA Broad Range Assay Kit, Life Technologies, Carlsbad, CA, USA). Purified DNA from 35 isolates was prepared for sequencing using Nextera XT DNA Library Prep Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 5μL of nucleic acid extract and 20μL of RT-qPCR reagent mix using cycling parameters ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 2.5μL of nucleic acid extract and 7.5μL of RT-qPCR reagent mix ...
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bioRxiv - Bioengineering 2021Quote: ... DNA contents were measured using the Quant-it PicoGreen double-stranded DNA Kit (Quant-iT™ PicoGreen™ dsDNA assay kit, Cat. No. P7589, Invitrogen). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated from snap-frozen (liquid nitrogen) tissues using a Qiagen RNeasy Plus Isolation Kit followed by cDNA synthesis (High-Capacity cDNA RT kit, Applied Biosystems, 4374966). RT-PCR was performed using TaqMan Universal Master Mix II (Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... CRISPR gRNAs were synthesized using a MEGAshortscript T7 Transcription Kit and purified using a mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA). Zebrafish codon-optimized cas9 was synthesized using a mMESSAGE mMACHINE Kit (Ambion ...
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bioRxiv - Neuroscience 2021Quote: ... rRNA were depleted from 1 μg of total RNA using the NEBNext rRNA depletion kit (Huma/Mouse) and ribo-depleted RNA was quantified by Quant-iT ™ RiboGreen ™ RNA Assay Kit (Invitrogen). Using a reverse transcriptase and random primers ...
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bioRxiv - Microbiology 2019Quote: ... Ilumina libraries were prepared using the Kapa Hyper prep kit as described (41) and quantified using Qubit dsDNA BR Assay Kit (Thermo Fisher Sc). Fragment size was assessed on a Fragment Analyzer (Advanced Analytical Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... The amplified band was purified using a Qiagen gel purification kit and TA cloning was performed using a TOPO™ TA cloning™ kit (Invitrogen). 12 clones were randomly picked for Sanger sequence analysis by the Advanced Genomics Core at the University of Michigan.
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated using the Rneasy Mini Kit (cat# 74134) and cDNA was synthesized using High Capacity cDNA Reverse Transcription Kit (ThermoFisher, cat#4368814). Quantitative PCR was performed on synthesized cDNA samples using ThermoFisher TaqMan Master Mix (cat# 4444556 ...
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bioRxiv - Neuroscience 2021Quote: ... Library quality was determined with the Agilent High Sensitivity DNA kit on an Agilent Bioanalyzer and concentrations measured with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher) on a QubitTM (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... and control mice (Shank3b+/+ and Cntnap2+/+) with RNeasy Mini Kit (QUIAGEN) and retro-transcribed to cDNA with a SuperScript™ VILO™ cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Bar-coded small RNA sequencing libraries were generated using the Ion Total RNA-Seq Kit v2 and the Ion Xpress RNA-Seq bar coding kit (Thermo Fisher Scientific). For the developmental course samples (Figure 1A) ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Genetics 2022Quote: ... The plasmid was extracted by Hybrid-Q™ Plasmid Rapidprep Kit (GeneAll, Korea) and sequenced using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, USA) on ABI 3130 instrument (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Bar-coded RNA libraries were generated according to the manufacturers’ protocols using the Ion Total RNA-Seq Kit v2 and the Ion Xpress RNA-Seq barcoding kit (Thermo Fisher Scientific). The size distribution and yield of the barcoded libraries were assessed using the 2200 TapeStation System with Agilent D1000 ScreenTapes (Agilent Technologies) ...
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bioRxiv - Physiology 2023Quote: ... Total IL-1β protein level was measured by mouse IL-1β/IL-1F2 DuoSet ELISA kit and first normalized to total protein level quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific), and then normalized to the sham group.
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bioRxiv - Molecular Biology 2022Quote: ... In vitro transcribed sgRNAs were purified and eluted with MEGAclear Purification Kit (LifeTechnologies, Carlsbad, CA) for the Cas9 102 nucleotide RNA products or with the mirVana miRNA isolation kit (Ambion, Austin, TX) for the Cas12a 44 nucleotide RNA products ...
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bioRxiv - Developmental Biology 2022Quote: ... Total expression levels of miR-675-3p and miR-675-5p were determined relative to snoRNA202 by using a separate RT kit (TaqMan MicroRNA Reverse Transcription Kit, Thermo Fisher Scientific), qRT-PCR primers (Assay Id 001232 ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted DNA concentrations were quantified using the Qubit dsDNA HS Assay kit and the Qubit ssDNA Assay kit (Thermofisher Scientific, USA).
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Physiology 2023Quote: ... Tau protein was also labeled separately with Alexa Fluor 555 using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were kept and protein concentration was estimated using the micro-BCA Protein Assay Kit (Pierce BCA Protein Assay Kit (Thermo Scientific: 23225)) ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was measured using the Pierce 660nm Protein Assay Kit or the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Microbiology 2023Quote: ... Quantification of RNA and DNA were performed using Qubit™ RNA Assay Kits and Qubit™ dsDNA HS Assay Kits (Invitrogen, Singapore), respectively ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cells using a Universal RNA purification kit (EURx) and reverse-transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2023Quote: FITC and Alexa Fluor 647 labelling of purified proteins was done by using FluoroTag FITC Conjugation Kit (MilliporeSigma, FITC1-1KT) and Alexa Fluor™ 647 Antibody Labeling kit (Invitrogen, A20186), respectively ...
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bioRxiv - Genomics 2023Quote: Qubit DNA Quantification Assay kit (High Sensitivity) or Quant-iT PicoGreen dsDNA Assay Kit with Lambda DNA standard (Invitrogen/Thermo Fisher Scientific)
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bioRxiv - Genomics 2023Quote: Qubit DNA Quantification Assay kit (High Sensitivity) or Quant-iT PicoGreen dsDNA Assay Kit with Lambda DNA standard (Invitrogen/Thermo Fisher Scientific)
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bioRxiv - Cell Biology 2023Quote: ... The yield of amplified libraries was measured on a Qubit 4 fluorometer using the Qubit high sensitivity DNA kit (HS DNA kit) (Thermo Fisher Scientific). The libraries were sequenced at Genomed (Warsaw ...
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bioRxiv - Developmental Biology 2024Quote: ... sgRNAs were made using the GeneArt Precision gRNA Synthesis Kit following the protocol provided in the kit (Invitrogen A29377, Supplemental table 10). The sgRNA were mixed with InvitrogenTM TrueCut Cas9 protein (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNAs of the cells were then extracted by the RNeasy Mini Kit and analyzed by the SYBR Green Real-Time PCR Kit (Thermo Fisher Scientific). Cyclophilin mRNA levels were utilized as the internal control.