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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 7H Cyclopenta c pyridin 7 one 5 6 dihydro 3 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Escherichia coli was grown at 37°C (or 28°C with extrachromosomal pMBsacB present) with shaking in Luria-Bertani (LB) medium (Fisher Scientific cat. # DF9446-07-5) supplemented with 300 µg/ml erythromycin and 150 µg/ml spectinomycin as needed for selection ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured in RPMI (24 h, 37°C, 5% CO2) and stained with MitoTracker Red FM (ThermoFisher Scientific, M22425; 30 min, 37°C). Grids were vitrified by plunge-freezing using a Leica EM GP2 grid plunger (Leica Microsystems ...
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bioRxiv - Genetics 2023Quote: ... 45 amplification cycles at 95°C for 5 sec and 60°C for 30 sec) in the Applied Biosystems 7300 fluorescence quantitative PCR system (Applied Biosystems, Foster City, CA, USA) according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... coli strain DH5α was grown at 37°C (or 28°C with extrachromosomal pMBsacB present) with shaking in Luria-Bertani (LB) medium (Fisher Scientific cat. # DF9446-07-5) supplemented with 300 μg/mL erythromycin or 50 mg/mL kanamycin as needed for selection.
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bioRxiv - Bioengineering 2022Quote: ... All the cells were cultured at 37°C in 5% CO2 using 5 mL of Dulbecco modified Eagle medium (DMEM, Thermo Fisher Scientific, MA, USA)–high-glucose (4500 mg of D-glucose/liter ...
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bioRxiv - Bioengineering 2022Quote: Constructs were incubated for 1 hour at 37°C with 3 mM Fluo3 AM calcium indicator (ThermoFisher Scientific) in differentiation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... 20 sec 72 °C) with fluorescent readings during annealing and elongation on a QuantStudio 3 machine (Thermo Fisher). Three reference genes were tested ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Tail and ear fragments from C57/B6 or mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNAs were fragmented for 3 minutes at 70 °C using a Zinc-based alkaline fragmentation reagent (AM8740, Ambion). RNAs were cleaned up using RNeasy MinElute Cleanup Kits (74204 ...
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bioRxiv - Bioengineering 2019Quote: ... Lysis was done in 2% SDS for 3 min at 95 °C in a thermomixer (Thermo Fisher Scientific), followed by digestion of DNA with Benzonase at 37 °C for 1.5 h ...
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bioRxiv - Developmental Biology 2019Quote: ... at 4°C for 1-3 days followed by secondary antibody (1:500, Thermo Fisher Scientific #A-21206) overnight at 4°C.
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bioRxiv - Biophysics 2023Quote: ... This was done by dissociating cells for 3 min at 37°C using TrypLE Express (ThermoFisher, Cat#: 12605010), neutralizing with 1:10 volumes (CDM) ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95⁰C for 3 min and loaded onto a SDS-polyacrylamide gel (4-12%, Invitrogen NuPAGE, #NP0336BOX). After size separation ...
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bioRxiv - Bioengineering 2023Quote: ... for 3 hours at 37°C and then in vitro transcribed under control of promoter T3 (Ambion AM1348) for 3 hours at 37°C and DNAse treated with TURBO-DNAse for 20 min at 37°C ...
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bioRxiv - Pathology 2023Quote: Tail and ear fragments from mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixtures were incubated at 25°C for 3 hours and then transferred to centrifugal filter (Thermo Fisher). The flow-through (FT ...
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bioRxiv - Genetics 2024Quote: ... and BSA (3%) for 4 hours at 4°C after which streptavidin-agarose UltraLink Resin (Thermo Fisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies, Grand Island, NY, USA) with recombinant human GM-CSF (Miltenyi Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670, 0.001% Brij35 ThermoFisher, #28316 ...
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bioRxiv - Microbiology 2021Quote: Cells were reverse-transfected (transfected while plating) in 6-well plates (3.5 -5 × 105 cells per well) using Lipofectamine RNAimax transfection reagent (Invitrogen), following standard protocols ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... 5 mM DTT) and then mixed with 50 mM (final 6 mM) disuccinimidyl dibutyric urea (DSBU) (Thermo Fisher Scientific). Crosslinking reaction was incubated on ice for 2 hours and then was quenched by adding 1M (final 50 mM ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were plated in 6 well plates and transfected with 5 μl Lipofectamine RNAiMAX transfection reagent and siRNAs (Invitrogen) at 50 nM final concentration according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... resulting from UA were determined via a membrane-permeant JC-1 dye (5, 50, 6, 60 -tetrachloro-1, 10,3,30 -tetraethylbenzimidazol-carbocyanine iodide, Molecular Probes). JC-1 dye accumulates in mitochondria in a potential-dependent manner ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with transfection complexes for 5-6 hours and then replated onto #1.5 coverslips (Warner Instruments) coated with Collagen IV (Gibco). Cells were imaged in DMEM/10% FBS ...
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bioRxiv - Immunology 2020Quote: ... animals previously injected with vaccine or PBS where injected 24 hrs before sacrifice in the same footpad with 30 μL of 0.5 mM 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen). For assessing migration after 24 hrs ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Neuroscience 2021Quote: ... incubated 5 minutes with at room temperature with 500 ng/ml 4’,6-Diamidino-2-Phenylindole Dilactate (DAPI; ThermoFisher) in DPBS and mounted in ProLong®Gold (ThermoScientific).
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... coverslips were stained for 5 min with 1 μg/ml 300 nM 4′,6-diamidino-2-phenylindole (Life Technologies) to visualize nuclei ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of each SF was diluted 7-fold in water and used for a Bradford protein assay (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA) and Direct-Zol RNA Mini Prep (Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... at ~10 mg ml-1 concentration and blotted for 5-7 s followed by plunge-freezing into liquid ethane using a Vitrobot MarkIV (Thermo Fisher Scientific, USA) operating at 100 % humidity ...
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bioRxiv - Biophysics 2021Quote: ... T cells were maintained at 37°C and 5% CO2 in RPMI 1640 media (Life technologies) supplemented with 10% FBS (MERCK) ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 mM Sodium Citrate (pH 5 at 25°C) and 0.2 U/µl RNase T1 (ThermoFisher). Reaction mixtures were incubated at 50°C for 10 min prior to being stopped by flash freezing in liquid nitrogen for 5 min and adding equivolume 90% formamide ...
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bioRxiv - Cell Biology 2020Quote: ... A375 cells were cultured at 37 °C with 5% CO2 in DMEM (Thermo Fisher, #12800-082) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with anti-cytochrome C (33-8200, 5 μg/ml, mouse monoclonal, Thermo Fisher) and anti-SPIRE1 (SA-2133 ...
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bioRxiv - Cell Biology 2019Quote: Cells were incubated at 37°C and 5% CO2 with MitoTracker-Red FM (100 nM) (Invitrogen) for 15 min to stain mitochondria and Hoechst 33258 (2.5 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ATCC HeLa CCL2 (ATCC) were cultured at 37°C and 5% CO2 in DMEM (Gibco 11965092) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... and cultured at 35°C with 5% CO2 in 7.4 g/L MEM (ThermoFisher, #11700-077) with the addition of (in mM unless labeled otherwise) ...