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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for 7H Cyclopenta c pyridin 7 one 5 6 dihydro 3 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Reactions were performed at 30°C for 10 min and terminated by addition of 0.4% SDS and 6 × loading buffer (Thermo Scientific). The reaction mixture was loaded onto an 0.8% agarose gel in 1x TAE (40 mM Tris ...
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bioRxiv - Pathology 2023Quote: ... The samples were then incubated for 24 hours at 37°C in a standardised volume (6 mL/g of tissue) of RPMI 1640 medium (Gibco) supplemented with antibiotics and antimycotics ...
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bioRxiv - Cell Biology 2023Quote: Binucleation analyses and detection of aberrant divisions were performed by heat-fixing cells on a microscope slide at 70 °C before staining with 4’,6-diamidino-2-phenylindole (DAPI) (SlowFade Diamond Antifade Mountant with DAPI, Invitrogen) and Calcofluor (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Immunology 2024Quote: ... Bacterial suspensions were further centrifuged at 3,000 rpm during 30 minutes at 6 °C and resuspended in the same volume of RPMI 1640 broth (Gibco, USA) for intranasal piglet infection ...
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bioRxiv - Pathology 2019Quote: ... The cells were maintained under standard cultural conditions at 37°C in an atmosphere of 5% CO2 and 5% O2 in a Heracell CO2 incubator (ThermoFisher Scientific, Waltham, MA) (28) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we incubated 3x10[6] cells in 1ml Schneider’s Medium including 10% FBS for 5 min at 25°C with or without 5 µg/mL puromycin (Gibco™ Sterile Puromycin Dihydrochloride). Cells were then washed twice and incubated for 50 min at 25°C ...
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bioRxiv - Genetics 2023Quote: Mouse Embryonic Fibroblasts were obtained from E12.5 mouse embryos and expanded for 10 days at 37 °C with 5% CO2 and 5% O2 in DMEM medium (Thermo Fisher Scientific, 31966021) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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bioRxiv - Physiology 2020Quote: Bone biopsies stored at -80°C between 1 and 7 days were placed into liquid nitrogen contained in a ceramic mortar sprayed with RNaseZap® (ThermoFisher Scientfic, MA, USA). Samples were pulverised using an RNaseZap-treated pestle ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated at 37 °C for 7 days with secondary Alexa Fluor 647 donkey anti-rabbit IgG (1:450; Thermo Fisher Scientific, Rockford, IL) in 3% donkey serum/PTwH and then washed in PTwH for 5 times.
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bioRxiv - Plant Biology 2024Quote: ... extracted with 3 mL methanol for 1 h shaking at 200 rpm at 37 °C in a 7 mL glass scintillation vial (Fisher Scientific 03-337-26). The methanol extracts were dried under nitrogen gas and resuspended in 3 mL of deionized water ...
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bioRxiv - Physiology 2019Quote: ... Hearts were immersed for one day in a chemical mixture obtained by mixing 25 wt% urea (U16–3, Fisher Scientific Hampton, NH, USA), 25 wt% N,N,N0,N0-tetrakis(2-hydroxypropyl ...
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bioRxiv - Microbiology 2020Quote: ... A 25 μl RT-PCR for the viral E gene was carried out using 5 μl of extracted RNA template using the AgPath-ID One-Step RT-PCR (Applied Biosystems). Samples were processed in duplicate ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 μL of this reaction was used for reverse transcription and preamplification of cDNA in a 5 μL one-step reaction using the RT enzyme from Taqman RNA-to-Ct (ThermoFisher, #4392938) and the Taqman Preamp Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: One milliliter of monomeric capsid (5 mg/ml) was dialyzed in SnakeSkin dialysis tubing (10K MWCO, Thermo Scientific, Waltham, MA, USA) using a buffer that was high in salt and contained a reducing agent (Buffer 1 ...
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bioRxiv - Neuroscience 2024Quote: ... and the primers listed in Extended Data Table 5 were annealed at 61degC on the Step One Plus Real-Time PCR System (Life Technologies). Fold-changes were calculated using the ΔΔCt method.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3× for 5 min each in vehicle and then incubated in vehicle containing IgG-subclass-specific secondary Abs for one hour (Thermo Fisher). Sections were then washed 3× for 5 min each in PB and mounted with Prolong Gold (Thermo Fisher cat#P36930 ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was co-transcriptionally capped using m7(3’OMeG)(5’)ppp(5’)(2’OMeA)pG capping reagent (Hongene Biotech) in a “one-pot” reaction followed by digestion with DNase I (Thermo Fisher). Linear mRNA was purified using Dynabeads MyOne Carboxylic Acid beads (Thermo Fisher).
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bioRxiv - Cancer Biology 2024Quote: ... and each treatment condition was labeled with one channel of TMT-11plex reagent (5 µl of 25 µg/µl reagent per reaction, prepared in dry acetonitrile, Thermo Scientific) for 1 h at 23 °C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by one to two weeks of selection with fresh medium containing 200-500 μg/ml Hygromycin (Invitrogen, ant-hg-5) or 7.5 μg/ml Blasticidin (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse transcribed and amplified in 5 reactions with 1 µg RNA template using SuperScript IV One-Step RT-PCR System with ezDNase (Invitrogen, 12595025) and the primers ...
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bioRxiv - Zoology 2021Quote: ... and with NanoDrop One (ThermoFisher) with target OD 260/280 and OD 260/230 ratios of 1.8 and 2.0-2.2 ...
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bioRxiv - Microbiology 2021Quote: ... One-step Ultra TMB (ThermoFisher) was then used for detection ...
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bioRxiv - Immunology 2021Quote: ... one labeled with AlexaFluor647 (Invitrogen), were used in this panel in order to increase specificity of the detection of SARS-CoV-2-specific B cells ...
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bioRxiv - Immunology 2021Quote: ... one labeled with AlexaFluor488 (Invitrogen), one labeled with AlexaFluor647 (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... spectrophotometric (Nanodrop One, Fisher Scientific) determination of purity ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli One Shot TOP10 (Invitrogen) were transformed with the resulting vector ...
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bioRxiv - Genomics 2024Quote: ... and NanoDrop One (Thermo Fisher).
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bioRxiv - Genetics 2024Quote: ... One ml of TRIzol (Invitrogen) was added to the cells and mixed thoroughly ...
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bioRxiv - Microbiology 2019Quote: ... The DNA was dissolved in 50 μl molecular grade water for 1 hour at 65°C and the DNA concentration determined using a NanoDrop™ One/Onec Microvolume UV-vis Spectrophotometer (ThermoFisher Scientific, USA).
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bioRxiv - Plant Biology 2021Quote: ... these were extracted and dialyzed against 25 mM Tris pH 7.5 at 4 °C and measured using a Nanodrop One (Thermo Fisher Scientific, Waltham, MA). Particles for cryo-EM were dialyzed against 20 mM NaOAc ...
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bioRxiv - Neuroscience 2021Quote: ... preparations were washed six times with PBT and incubated for one night at 4°C with secondary antibodies goat anti-rabbit Alexa 488 (Invitrogen, USA; 1:250) and goat anti-mouse DyLight 649 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... Quantification of the Alexa Fluor® 647 ratio per tetanus toxin was obtained by scanning measurements using the NanoDrop One c device (Thermo Fisher Scientific) with 280 and 650 nm readings ...
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bioRxiv - Neuroscience 2023Quote: ... brains were equilibrated at -20 °C for one hour and 15 μm sections were collected using a cryostat and mounted on Superfrost Plus slides (Fisher Scientific, catalog # 22037246). RNAScope assays were performed following the recommendations of the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality and concentration were estimated with NanoDrop One C spectrophotometer and Qubit 4MT with a dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA). Total of 83 Lonsdalea quercina strains (one strain per tree ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were incubated at 4°C for one day in PBT with 0.002% Streptavidin conjugated to Alexa Fluor 633 (ThermoFisher Scientific, Waltham, MA, USA), then washed two times in PBT and two times in PB ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then washed twice with Blocking One (Nacalai Tesque, 03953-95) and incubated overnight at 4 °C with primary antibodies diluted in 10% goat serum (Thermo Fisher Scientific, 16210064)/Blocking One ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was suspended in RNase-free water and the concentration and purity of the samples was determined using a Nanodrop instrument (ThermoFisher, Nanodrop One C). Library preparation and RNA sequencing were performed as a service by GENEWIZ/AZENTA and sequenced on a Novaseq 6000 instrument (Illumina).
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bioRxiv - Immunology 2024Quote: ... and OtsuR747 (5’- TATGCCTGAGTAAGATACGTGAATGGAATT-3’) primers (IDT, Coralville, IA) and detected with the probe OtsuPr665 (5’-6FAM- TGGGTAGCTTTGGTGGACCGATGTTTAATCT-TAMRA) (Applied Biosystems, Foster City, CA) by SsoAdvanced Universal Probes Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS 3-5 times for 5 minutes each and mounted with ProLong Gold Antifade Mountant (#P36930, Thermo Fisher Scientific, Waltham MA). Confocal images were taken with the 20x and 40x (oil ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: Membrane potential was evaluated using the image-iT TMRM (tetramethylrhodamine, methyl ester) reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were then incubated with Tetramethylrhodamine-methyl-ester (TMRM) (Thermo Fisher Scientific, United States) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were cleared in methyl salicylate and mounted using DPX mountant (Fisher Scientific, #18404). Images of whole mounts were captured at different magnifications using stereoscope (Magnus MSZ-TR).
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bioRxiv - Microbiology 2022Quote: Membrane potential was evaluated using the image-iT TMRM (tetramethylrhodamine, methyl ester) reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... gels were incubated with 10 µM BODIPY TR Methyl Ester (Thermofisher, catalog no. C34556) in 1× PBS for 1 h on a rocking platform at RT and washed three times with 1× PBS for 30 min each ...