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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 7H Cyclopenta c pyridin 7 one 5 6 dihydro 3 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... for 3–5 s and then washed thoroughly in phosphate-buffered saline (PBS) (pH 7.4, Gibco, USA). Single zona pellucida-free embryos were carefully pipetted and placed into individual tubes containing 2 μL PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 2.5 mM probenecid (Life-Technologies: catalog # P36400) and 5 µM Fluo-3 (Life Technologies; catalog # F-14218). Cells were incubated at 37°C for 30 minutes before being centrifuged and resuspended in modified L-15 media containing 2% FBS and 2.5 mM probenecid ...
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bioRxiv - Cell Biology 2019Quote: ... PKC-specific custom siRNA targeting to endogenous human PKCα (5’-CAGAAGAACTGTATGCAAT-3’) was purchased from Ambien (ThermoFisher). To perform knockdowns ...
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bioRxiv - Immunology 2020Quote: ... containing a 5′-CACC-3′ sequence required for directional TOPO cloning in pENTR/D-TOPO (Invitrogen, USA), and a reverse primer [“Tgd057 R HA stop” 5’-TTACGCGTAGTCCGGGACGTCGTACGGGTACTCGACCTCAATGTTGTATTC-3’] ...
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bioRxiv - Cell Biology 2019Quote: ... actin was incubated with 3-5 molar excess amount of Alexa Fluor 488 C5 maleimide (Invitrogen, #A10254) or EZ-Link Maleimide-PEG2-Biotin (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... (RV-5′-GCCGTGGGTACTGAGAAAG-3′) and the fragments were bi-directional sequenced using (3130 Genetic Analyser; Applied Biosystems). Further the results were compared with the reference sequence of ARHGEF40 gene using NCBI-BLAST program and the chromatogram was analysed using Chromas lite (2.1 ...
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bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Immunology 2022Quote: ... Probe: 5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-NFQ-MGB-3’ (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2022Quote: ... Sections were washed 3× for 5 min each in PB and mounted with Prolong Gold (Thermo Fisher) and Deckglaser cover glass (Cat#NC1776158) ...
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bioRxiv - Cell Biology 2022Quote: ... 5′-RACE and 3′-RACE were done using the GeneRacer method according to the manufacturer’s instructions (Invitrogen). Briefly ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′) and KAPA HiFi HotStart ReadyMix (Thermo Fisher Scientific), cleaned up using RNAClean XP SPRI beads three times ...
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (ThermoFisher SuperSignal West) and imaged using a ChemiDoc (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... At 3 hours post infection (hpi) cells were seeded on 5 mm round glass coverslips (Thermo Scientific) and mounted on a sample holder specially designed for LLSM ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA (5′-TGTGAATCGTAGGAGCGCTG-3′) was synthesized via in vitro GeneArt™ Precision gRNA Synthesis Kit (ThermoFisher, A29377) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The 5’ homology arm-Linker-mAID-3’UTR fragment was synthesized using Genestrings® (Thermo Fisher Scientific). The Cas9-NCAPH2-gDNA plasmid and the two template plasmids (BSR or Hygro ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were passaged every 3-5 days at 70-80% confluence using 0.25% Trypsin-EDTA (Life Technologies). Cells were grown at 37°C in 5% CO2.
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bioRxiv - Biochemistry 2024Quote: ... and WNK1 (5’ CAGACAGUGCAGUAUUCACTT 3’) were transfected into HDMEC using Lipofectamine RNAiMax reagent (Thermo Fisher Scientific, 13778150). After 48 (TSC22D1 ...
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bioRxiv - Neuroscience 2024Quote: ... A guide RNA targeting exon 41 of the LRRK2 gene (5’-CTCAGTACTGCTGTAGAATG-3’) was commercially synthesized (ThermoFisher) and used to form ribonucleoprotein (RNP ...
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bioRxiv - Developmental Biology 2022Quote: ... injected fluorescent oocytes were selected and matured with 1-methyl adenine (3.0 μM Acros Organics). Eggs were fertilized with diluted sperm ...
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bioRxiv - Plant Biology 2021Quote: ... 30 μL of N-methyl-N-trimethylsilyltrifluoroacetamide plus 1% trimethylchlorosilane (MSTFA + 1% TMCS, Thermo Scientific) was added to the MSTFA vials ...
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bioRxiv - Biophysics 2021Quote: ... A Methyl-β-Cyclodextrin solution (200 mM) in Live Cell Imaging Solution (Thermo Fisher Scientific) was added to the cholesterol lipid film at a molar ratio of 10 to 1 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then pegylated with MS(PEG)8 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) for 15 minutes prior to grid preparation.
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bioRxiv - Physiology 2024Quote: ... for 1 hour and then incubated with Calcium Orange AM methyl ester (Invitrogen Cat. # C3015) + 0.01% Pluronic acid (Invitrogen Cat ...
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bioRxiv - Pathology 2022Quote: Cell viability of the MRC-5 cells cultured on LdECM and Ru-LdECM hydrogels was assessed after 1 and 7 days using Calcein AM (Thermo Scientific, Breda, the Netherlands) to stain live cells and propidium iodide (PI ...
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bioRxiv - Microbiology 2022Quote: ... and gene-specific primers were used to perform quantitative reverse transcription-PCR (qRT-PCR) on 5 ng/μL cDNA in a Real-Time PCR System (Applied Biosystems ViiA 7, USA). Table S2 lists the primers used in qRT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed 3 times for 15 min at 67°C in 1X Hybridization Stringency Wash Buffer (Thermo Fisher), developed ...
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bioRxiv - Molecular Biology 2019Quote: ... and (3) 110,000 × g for 2 hours at 4°C with a Sorvall Discovery SE ultracentrifuge (Thermo Fisher Scientific) with an AH-650 rotor (k factor ...
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bioRxiv - Microbiology 2020Quote: ... Forty cycles of amplification at 60 °C were conducted in a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Amplification curves were analyzed using QuantStudio Design & Analysis Software v1.5.1 ...
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bioRxiv - Microbiology 2021Quote: ... After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco) supplemented with 5% New Born Calf Serum (NBCS ...
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bioRxiv - Cell Biology 2021Quote: ... under 5% CO2 at 37°C in humidifying conditions and passaged every 2-3 days using 0.25% Trypsin-EDTA (25200056, Gibco). Cells were routinely tested for mycoplasma ...
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bioRxiv - Microbiology 2021Quote: ... a recodonized TgHypo cDNA sequence fused to a C-terminal 3×HA tag was synthesized by GeneArt (Life Technologies). This fragment was cloned into the BamHI and HindIII sites of a modified version of the parental plasmid p5RT70loxPKillerRedloxPYFP-HX3 ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Bioengineering 2024Quote: ... and inflated using a needle and syringe with warm (42°C) 3% low-melting point agarose (ThermoFisher #BP165-25) diluted in DMEM-H (Gibco #11965-092) ...
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bioRxiv - Neuroscience 2023Quote: Samples were heated to 95°C for 3 minutes in loading buffer NuPAGE LDS Sample Buffer (4x) (Invitrogen NP0008) with 5% β-Mercaptoethanol then loaded on 4-20% Criterion TGX Precast Midi protein gels (Bio-Rad #5671094) ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 72°C for 20 seconds and a melt curve stage on a QuantStudio 3 quantitative PCR system (Thermo Fisher) and each assay was performed in triplicate ...
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bioRxiv - Microbiology 2020Quote: ... one-step RT-PCR was carried out using a One-step RT-PCR detection kit (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... One sample was stored dry and one was stored in RNAlater (Thermo Fisher Scientific, Waltham, Massachusetts). All samples ...
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bioRxiv - Neuroscience 2022Quote: ... The RNA concentration of these samples were quantificated by NanoDrop One (Thermo Scientific, ND-ONE-W). 2 ug RNA per sample were used to reverse transcript the cDNA by QuantScript RT Kit (TIANGEN Biotech ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Immunology 2021Quote: ... monocyte-derived DCs that were transfected with hACE2 were seeded at 0.5×106 cells/mL in a 6-well plate and transfection was performed with Lipofectamine LTX and PLUS reagents (Invitrogen) according to the manufacturer’s instructions for primary cells ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Cell Biology 2022Quote: 100,000 BHK cells were plated into a 6-well plate and transfected with 5 nM of siRNA pool (siTOOL) against Uhrf1 or Usp7 by Lipofectamine 3000 (Invitrogen). After 24 or 48 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... E8 medium was replaced daily and hiPSC were gently passaged every 5-6 days by mechanical selection or bulk passaged using non-enzymatic reagents (i.e., Versene solution (ThermoFisher Scientific) or Phosphate-Buffer-Saline (PBS)-based enzyme-free cell dissociation buffer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted 1:5 in TE buffer and 6% were mixed with TaqMan Universal PCR Master Mix (Applied Biosystems). The sample mix and TaqMan assays were loaded separately into the wells of 96.96 Gene expression Dynamic Arrays (Fluidigm ...
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bioRxiv - Bioengineering 2021Quote: ... Droplets of 5 μL cell-Matrigel mixture were manually deposited onto a high performance #1.5 glass bottom 6-well plate (Fisher Scientific). Data acquisition was performed using a confocal microscope (Nikon A1R HD25 ...