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Citations for Thermo Fisher :
1801 - 1850 of 3721 citations for Rubella Virus VLP strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... we exchanged the medium in the filtered virus collections three times with Opti-MEM I (1X) + GlutaMAX (Gibco) using an Amicon Ultra-15 column (100 kDa cutoff) ...
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bioRxiv - Microbiology 2022Quote: ... quantitative polymerase chain reaction (qPCR) was performed using TaqMan Fast virus 1 Step PCR Master Mix (Applied Biosystems); a standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... Reverse-transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo (dT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative real-time PCR was performed using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), 2019-nCoV RUO Kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated to passage three on Vero E6 cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (10,000 IU/mL ...
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bioRxiv - Microbiology 2019Quote: FVG-R infections were performed by inoculating A549 cells with virus diluted in Opti-MEM I (Invitrogen, 31985070) supplemented with 0.2% FBS ...
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... Samples KAB_3 and KAB_7 were reprogrammed using Sendai virus (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... Transmissible gastroenteritis virus [TGEV] and Swine deltacoronavirus [SDCoV] (VetMAX™ PEDV/TGEV/SDCoV, Thermo Fisher Scientific, MA, USA) and Senecavirus A [SVA] (EXOone Seneca Virus Valley ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo(dT ...
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bioRxiv - Synthetic Biology 2022Quote: ... undiluted SINV-containing supernatants were combined with the TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher, #4444434) and the appropriate primer-probe sets in technical triplicates ...
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bioRxiv - Microbiology 2022Quote: ... Premixes were prepared for each amplification reaction using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... C and S genes of the Hepatitis B virus (accession number: KY00323035) was ordered from GeneArt (ThermoFisher Scientific) and is available from Addgene (Addgene # 179155).
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bioRxiv - Neuroscience 2023Quote: ... C-Myc) introduced by Sendai virus according to the manufacturer’s instructions (Cytotune iPS 2.0 Sendai reprogramming kit, ThermoFisher) or by using an episomal method ...
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bioRxiv - Cell Biology 2023Quote: ... After a brief culture to expand the erythroblast population a Sendai virus based approach (CytoTune 2.0 Kit, Invitrogen) was used to express the Yamanaka factors without any genomic integration (27) ...
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Epstein–Barr virus-transformed lymphoblasts were obtained from Coriell Cell Repositories and were grown in RPMI 1640 (Gibco) supplemented with 10% FBS and penicillin/streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA synthesis and PCR were performed using TaqMan™ Fast Virus 1-Step Multiplex Master Mix (#4331182; ThermoFisher). Probes were used to detect the mouse transcripts of Htr2a and Gapdh (Mm00555764_m1 and Mm99999915_g1 4448892 ...
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bioRxiv - Cell Biology 2023Quote: ... infected with Sendai virus containing Yamanaka factors from the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (ThermoFisher, A165167) according to manufacturer recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... 8 ml P4 virus was used to infect 800 ml cultures of Expi293F GnTI-Cells (Thermo Fisher Scientific) cells at 2-3 × 106 cells supplemented with 1% Fetal Bovine Serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080], 5% FBS [Hyclone SH30070.03HI] and 1% Penn-Strep [Gibco 10378016]). Monoclonal antibody dilution plates were prepared similarly to serum dilution plates ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 10-fold serial dilutions of the virus for 1 h and overlaid with 0.55% methylcellulose or 1.0% cellulose in minimal essential medium (MEM) (Gibco) supplemented with 2% FBS (Corning ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Genomics 2024Quote: ... Virus was produced by transfecting HEK293T cells with the plasmid and the Lipofectamine 3000 kit (Thermo Fisher Scientific). U87MG were transduced with the supernatant containing the NY-ESO-1 virus ...
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bioRxiv - Microbiology 2024Quote: ... and CC81 (feline cell line transformed by mouse sarcoma virus ECACC 90031403) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-high glucose (GlutaMAX, Gibco) supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Virus production medium contained DMEM supplemented with L-glutamine and sodium pyruvate and 10% heat-inactivated FBS (Gibco). Viral supernatants collected roughly 48- and 72-hours post-transfection were sterile-filtered through a 0.45-micron filter and concentrated in 100kDa molecular weight cutoff filters.
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bioRxiv - Cell Biology 2020Quote: F-Actin was stained with phalloidin conjugated to Rhodamine (Invitrogen, Cat. #R415, 1:500 dilution). The cell membranes were labelled with CellMask Orange Plasma Membrane Stain or CellMask Deep Red Plasma Membrane Stain (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... F-actin was detected using Alexa Fluor- 488 and -647-conjugated Phalloidin (Invitrogen, 1:200). Fluorescence imaging was performed using an inverted Zeiss LSM880 confocal microscope with a fast-mode Airyscan detector and a 63x /1.4 NA ...
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bioRxiv - Molecular Biology 2020Quote: ... A549 cells (ATCC CCL-185) were maintained in Ham’s F-12K medium (Gibco, 21127-022) supplemented with 10% v/v FBS (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... Filamentous actin (F-actin) was labelled using phalloidin Alexa-Fluor 568 (1:200, Life Technologies). All antibodies used in this study have previously been extensively used and validated for immunofluorescence studies.
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bioRxiv - Cell Biology 2022Quote: ... and F-Actin was stained with 1/400 Rhodamine or AlexaFluor-488-conjugated phalloidin (ThermoFisher).
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bioRxiv - Immunology 2019Quote: ... HEK 293-F cells were transiently transfected using the FreeStyle MAX293 expression system (Life Technologies) and proteins purified as for CHO-K1 cells.
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... were obtained from ThermoFisher/Invitrogen (N7800-100) and cultured in KnockOut™ D-MEM/F-12 medium (ThermoFisher 12660012) supplemented with 2mM L-glutamine (Gibco 25030081) ...
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bioRxiv - Bioengineering 2019Quote: ... Secondary antibodies F(ab) goat anti mouse AF555 and goat anti rabbit AF488 (Life Technologies) were used at 1:500 dilution ...
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bioRxiv - Cancer Biology 2019Quote: Chinese hamster ovary (CHO) cells (ATCC, CCL-61) were maintained in F-12K medium (Gibco) with 10% fetal bovine serum supplementation ...
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bioRxiv - Cancer Biology 2020Quote: ... 25mM HEPES and 0.1%Pluronic F-68) containing 1 μg/mL anti-CD28 antibody (Invitrogen).
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bioRxiv - Immunology 2021Quote: ... After 45 minutes digested lungs were decanted in complete DMEM/F-12 (Gibco, Life Technologies) with DNase I (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... After 45 minutes digested lungs were decanted in complete DMEM/F-12 (Gibco, Life Technologies) with DNase I (Qiagen ...
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bioRxiv - Synthetic Biology 2021Quote: FreeStyle 293-F and FreeStyle CHO-S cell lines were obtained from Invitrogen (Carlsbad, CA) and cultured in FreeStyle 293 Expression Medium and complete FreeStyle CHO Expression Medium (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... DYNAmo Color Flash SYBR Green qPRC kit (cat# F-416L) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... scaffolds were washed and stained for F-actin (488 Alexa Fluor Phalloidin, Thermo Fisher Scientific) for 1 h ...