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Citations for Thermo Fisher :
1601 - 1650 of 3721 citations for Rubella Virus VLP strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Wild-type mumps virus (MuVi/Utrecht.NLD/40.10; genotype G) was grown on Vero cells in DMEM (Gibco) with 2% FBS ...
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bioRxiv - Immunology 2021Quote: ... Reactions were conducted with 5 μL RNA and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems) with 500 nM primers and 200 nM probes ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfection and virus infection: Expression constructs were transfected into cells using Lipofectamine 2000 Transfection Reagent (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The genomic titer of each virus was determined by quantitative PCR using the ABI 7700 (Applied Biosystems) and primers specific to the WPRE ...
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bioRxiv - Immunology 2020Quote: ... Virus detection was performed using One-Step RT-PCR SuperScript(tm) III Platinum(tm) Kit (Life Technologies). Thermal cycling was achieved at 55°C for 10 minutes for reverse transcription ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells (ATCC) were cultured for virus production in Dulbecco’s Modified Eagle Medium: Nutrient Mixture (DMEM) (GIBCO) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids encoding two vaccinia virus capping enzyme subunits (pCAG-D1R and pCAG-D12L −0.8μg each) using 16μL Lipofectamine 2000 (Invitrogen) per transfection reaction in a total volume of 200μL of Opti-MEM (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR analysis was performed using Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were generated using the Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) carrying the Yamanaka reprogramming factors OCT3/4 ...
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bioRxiv - Neuroscience 2023Quote: ... medium containing virus was processed by centrifugation (Sorvall WX-90 Ultracentrifuge and SureSpin 630 rotor; ThermoFisher Scientific), using a sorbitol cushion (20% sorbitol ...
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bioRxiv - Neuroscience 2023Quote: Expanded PBMCs were transduced Sendai virus reprogramming vector – according to CytoTune® 2.0 Sendai (Thermo Fisher Scientific). Before cells infection ...
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bioRxiv - Molecular Biology 2023Quote: ... purified gRNA from benzonase-treated virus material was treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, USA), and analyzed using an Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... The PBMCs were reprogrammed by using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming kit, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used: influenza A virus nucleoprotein (NP, PA5-32242, 1:3000, ThermoFisher Scientific), XPO1 (46249S ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Microbiology 2023Quote: ... Infectious virus was reconstituted by transfecting ARPE-19 cells with BAC DNA and Lipofectamine 3000 (ThermoFisher L3000001); high titer stocks generated after three passages in ARPE-19 cells were used for this project ...
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bioRxiv - Microbiology 2023Quote: ... infected cells were stained with primary antibody (Vaccinia Virus Polyclonal FITC Antibody ThermoFisher PA1-73191, 1:1000) and Hoechst 33342 (1:10,000) ...
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bioRxiv - Biochemistry 2023Quote: ... virus was harvested by collecting supernatant and filtering it through 0.45µm PES sterile filter (Thermo Fisher, 2954545). Virus was concentrated by ultracentrifugation with a 20% sucrose cushion ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed twice with PBS and then incubated with virus suspended in infectious medium (DMEM; Invitrogen) supplemented with 35% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... This virus mixture was used to infect 1.8–2.4 liters of High Five cells (Thermo Fisher Scientific) at a cell density of 3 × 106 per ml for 60 h at 27°C ...
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bioRxiv - Cell Biology 2023Quote: ... from a cDNA library of HCT116 which was prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen), as described previously (Ninagawa et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells used for the production of virus were cultured in DMEM supplemented with 2mM glutamine (Gibco), 10% Tetracycline-free FBS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... cells were infected with virus diluted in 10 mL of Opti-MEM (ThermoFisher Scientific, Waltham, MA, USA) and incubated for 1 h at 37°C to allow for virus adsorption ...
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bioRxiv - Microbiology 2024Quote: ... and the virus was incubated with 25 μg of Alexa Fluor 488 NHS Ester (succinimidyl ester; Invitrogen) for 1 hour at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... followed by APC-conjugated F(ab’)2-goat anti-mouse secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... F-12K medium (ATCC® 30-2004) and Keratinocyte SFM media (Invitrogen, Carlsbad, CA) respectively ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding S variants were transiently transfected into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Cell Biology 2019Quote: ... and stained for F-actin with Texas Red-coupled phalloidin (1/200, Molecular Probes) and for nuclei with DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... F-actin was stained using the Alexa Fluor 488 Phalloidin (A123791, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... F-actin was stained with Alexa Fluor 647-conjugated phalloidin (1:50; Life Technologies). Images were acquired using LAS X software on a Leica TCS SP8 confocal microscope system using a HC PL APO 63×/1.40 objective.
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bioRxiv - Cell Biology 2020Quote: ... and F(ab’)2 goat anti-mouse Alexa Fluor 633 (Molecular Probes, 1:1000).
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bioRxiv - Cell Biology 2020Quote: ... Filamentous (F)-actin was labelled with directly conjugated Alexa Fluor 568 Phalloidin (ThermoFisher Scientific). The DNA-binding dye Hoescht 33258 (Molecular Probes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F-actin was stained with 0.66 units/mL Alexa 647-phalloidin (Life Technologies A22287) when combined with FM 1-43 FX ...
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bioRxiv - Developmental Biology 2020Quote: ... F-actin was visualized by incubating embryos with Alexa Fluor 647-conjugated phalloidin (Invitrogen) in dilution buffer ...
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bioRxiv - Cell Biology 2022Quote: RPE1 cells (hTERT-RPE1, RRID:CVCL_4388) were cultured in DMEM/F-12 medium (ThermoFisher Scientific) supplemented with 10 % fetal calf serum (ThermoFisher Scientific) ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... which was replaced on the next day by SC medium (DMEM/F-12 [Gibco] ...
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bioRxiv - Developmental Biology 2022Quote: ... F=Flag (https://emb.carnegiescience.edu/drosophila-gateway-vector-collection) using the Gateway cloning system (Invitrogen). AWG-MCS plasmids were used as GFP control for IP experiments.
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bioRxiv - Genomics 2020Quote: ... followed by AlexaFluor 647 F(ab’)2 goat anti rabbit IgG (H+L) (Invitrogen), CD3 (Clone UCHT1 ...
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bioRxiv - Microbiology 2019Quote: ... to visualize DNA and Texas Red-X phalloidin for F-actin filaments (Thermo Fisher). Images were acquired using a63Xoil objective on an EVOS FL fluorescent microscope.
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bioRxiv - Cell Biology 2019Quote: ... F-actin was stained with Alexa Fluor 488-conjugated phalloidin (1:50, Life Technologies) for confocal imaging.
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bioRxiv - Molecular Biology 2019Quote: ... The cells were maintained in phenol red-free DMEM/F-12 (Gibco, Life Technologies) supplemented with 5% charcoal-dextran stripped fetal bovine serum (Gemini Bio-Products ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were maintained in phenol red-free DMEM/F-12 (Gibco, Life Technologies) supplemented with 5% charcoal-dextran stripped fetal bovine serum (Gemini Bio-Products ...
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bioRxiv - Developmental Biology 2019Quote: ... F-actin was stained with AlexaFluor®647 Phalloidin (Thermo Fisher Scientific; 1:100) and nuclei were stained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488® phalloidin was used to selectively stains F-actin (Molecular Probes).
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bioRxiv - Biophysics 2021Quote: ... F-actin was stained with Alexa Fluor 532-labeled phalloidin (A22282; Invitrogen, Carlsbad, CA) and the DNA with DAPI (Sigma-Aldrich).