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Citations for Thermo Fisher :
1801 - 1850 of 3296 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were subsequently washed with TSB and incubated with WGA tagged with Alexa Fluor 488 (WGA-488, Invitrogen) at a final concentration of 2 μg/ml for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Transgenic S2 cells carrying the SIN3 187 transgene tagged with an HA epitope were grown in medium containing 0.1 mg/ml penicillin and streptomycin (Gibco). For treatment with CuSO4 ...
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bioRxiv - Plant Biology 2023Quote: ... Epitope-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... mYFP tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with uLimch1 and mLimch1 epitope tagged expression plasmids using Lipofectamine 3000 following the manufacturer’s protocol (catalog number L3000015, Invitrogen). mLimch1 and uLimch1 epitope tagged expression plasmids were commercially synthesized by GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... the C-terminal HA-tagged Cas12f/sgRNA-encoding plasmids were transfected into HEK293T cells by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed twice with PBS and stained with AlexaFluor 488-tagged donkey-anti-goat (Molecular Probes, 1:100), Cy3-tagged donkey-anti-chicken (Jackson Immuno ...
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bioRxiv - Biophysics 2023Quote: ... and the 3C protease was removed using Dynabeads magnetic beads designed for His-tagged protein isolation (Thermo Fisher, 10103D). The supernatant from the magnetic bead pulldown was then concentrated using Amicon 10 kDa centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Synthetic Biology 2023Quote: Tetra-cysteine (TC) tagged proteins (CCPGCC) were site-specifically labelled using the TC-ReAsH II TC detection dye (Invitrogen). TC-HERD-2.2–TnaA and TC-HERD-2.2–FMO (100 μM ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were-donkey anti-rabbit or donkey anti-mouse tagged with either Alexaflour488 or Alexafluor568 (1:1000; Invitrogen) or goat anti-rat Alexaflour350 (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged TpPyShell together with TEV protease was dialyzed overnight at 4°C in SnakeSkin dialysis tube (Thermo Scientific) against dialysis buffer (50 mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were gently mixed with a 2x solution such that the final concentrations were 0.5mg/mL Alexa Fluor 647-tagged 10,000 MW dextran (Invitrogen #D22914), 200nM caged fMLP (NEP) ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were seeded in 6-well plates and transfected with 0.25μg LgBiT-miniG (miniGs or miniGsq45) or LgBiT-β-arrestin240 and 0.25μg SmBiT-tagged receptor plasmids using 3µL Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... samples were washed and incubated with donkey anti-mouse secondary antibody tagged with Alexa Fluor 647 (Thermo Fisher #A32787) diluted in fresh blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... or Flag-tagged proteins were generated by inserting PCR-amplified fragments into the pcDNA3.1(+) mammalian expression vector (V79020, Invitrogen). Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Biochemistry 2019Quote: ... The cells were then incubated using FITC-labelled goat-anti-human IgG-FC antibody (ThermoFisher Scientific) (200 ng/ul ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cell density was measured as an optical density (OD, 595 nm; Multiscan FC Thermo Scientific) and colony forming units per ml (CFU/ml ...
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bioRxiv - Microbiology 2020Quote: ... RBD-Fc was biotinylated with EZ‐link sulfo‐NHS‐LC‐biotin (Thermo Fisher Scientific, Waltham, MA) (RBD-Fc-Bio) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with final 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest, Nuaillé, France) and antibiotics (Gibco, Waltham, MA, USA). The human NSCLC cell line NCI-H23 (H23 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco). Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells ...
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bioRxiv - Biophysics 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... S2R+ Cells (2 × 105) were seeded in Schneider medium plus 10% FCS (Gibco 21720024, Sigma F9665) in a 24-well plate ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 % fetal calf serum (FCS) (Biochrom, Ltd., Berlin, Germany) and 1 % penicillin-streptomycin (Invitrogen). The human colorectal adenocarcinoma cell line Caco-2 was provided by Jindrich Cinatl Jr ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were pre-incubated with Fc-Block (eBioscience, Thermo Fisher Scientific, Les Ulis, France) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and absorbance were subsequently measured at 450 nm using a Multiskan™ FC photometer (Thermo Fisher).
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with anti-CD16/32 (Fc Block) antibodies and Fixable Live/Dead stain (Invitrogen) for 15 min before staining with antibodies for surface markers in 2% BSA/PBS buffer for 30 mins on ice ...
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bioRxiv - Immunology 2019Quote: ... Cells were cultured in DMEM (Wisent) or RPMI1640 supplemented with 10% FCS and penicillin/streptomycin (Gibco). Media were supplemented with 200 UI/mL IL-2 (Proleukin – Novartis Pharmaceuticals ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% (v/v) heat-inactivated Hyclone fetal calf serum (FCS; Thermo Scientific, UT, USA), 2mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
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bioRxiv - Microbiology 2020Quote: COS7 and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... washed in TBS and blocked with StartingBlock T20 Blocking buffer containing Fc blocker (Thermo Scientific, 37543). Sections were incubated with unconjugated L ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco ...
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... was measured as absorbance at 450 nm (OD450) by Spectrophotometer (Thermo Scientific, Multiskan FC Microplate Photometer). The titers were defined from the reciprocal value of the absorbance at OD450.
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following the manufacturer’s instructions.