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Citations for Thermo Fisher :
1601 - 1650 of 3296 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HA-glmS-tagged parasite lines were coated on diagnostic slides (Epredia; Thermo Fisher Scientific; Waltham, US) and air-dried ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Biochemistry 2022Quote: Cloning of full-length N-terminal strepII-tagged hItln1 into the pcDNA4/myc-HisA vector backbone (Life Technologies) was described previously (14) ...
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bioRxiv - Cell Biology 2023Quote: ... To visualise the cell surface with Alexa488-tagged wheat germ agglutin (Thermo Fisher Scientific, UK, 1:2000 dilution) an incubation was done prior to permeabilization ...
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bioRxiv - Molecular Biology 2023Quote: ... Genes encoding tagged and untagged SOSIP.664 Env homotrimers were expressed by transient transfection of Expi293 cells (ThermoFisher). Env heterotrimers were purified from co-transfections involving a 20:1 expression plasmid DNA ratio of untagged to tagged Env constructs ...
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bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged β-catenin variants were expressed in transient transfection assays using the pCDNA3.1 vector (Thermo Fisher Scientific). A plasmid expressing human β-catenin containing a S33Y mutation (pCDNA3-S33Y ...
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bioRxiv - Molecular Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5’ ends were synthesized by Thermo Scientific, while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
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bioRxiv - Bioengineering 2024Quote: GFP-HUVECs were added to well 1 and GFP-HUVECs co-tagged with Hoechst (NucBlue Live ReadyProbes, Invitrogen) were added to wells 2-4 ...
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bioRxiv - Cell Biology 2023Quote: ... strep tagged APC/C was pre-incubated on ice with streptavidin conjugated Alexa Fluor 700 (ThermoFisher Scientific S21383) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Bioengineering 2024Quote: ... cells were either transduced to include a fluorescent reporter or fluorescently tagged using Hoechst (NucBlue Live ReadyProbes, Invitrogen) or general membrane cell tracker (Cellvue Claret Far Red ...
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bioRxiv - Biochemistry 2024Quote: Construct containing Avi-tagged HormR/GAIN domains of ADGRL3 was expressed in High Five cells (Thermo Fisher, B85502) grown in Insect-Xpress medium (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged: The lysate was mixed head-over-head with Ni-NTA agarose resin (Thermo Scientific™, USA) for 60 min at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: Lamprey embryos were injected with MYC-tagged lamprey (pou5) or Xenopus (pou5f3.1, pou5f3.2, pou5f3.3) mRNA and a FITC dextran tracer (ThermoFisher AAJ6360622) into one blastomere at the two-cell stage at the same concentration used for injections in Xenopus ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001) was used ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... T1249-Fc were expressed by transient transfection in 293-6E (CNRC) or Expi293 (ThermoFisher Scientific) cells and purified from transfected cell supernatants using a HiTrap MabSelect SuRe column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... absorbance at 405 nm was measured in a MultiSkan FC microplate reader (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Both compensation controls and samples were washed twice with 200μL FACS buffer (2% FCS (Gibco) in PBS (Gibco)) ...
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bioRxiv - Immunology 2020Quote: ... ECD region of mouse CLEC2 was cloned into mammalian expression vector pFuse-rIgG-Fc (Invitrogen). The construct was used to stable transfect HEK-293T cells with PEI method ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... 10% FCS and 100 units / ml of penicillin and 100 μg / ml of streptomycin (Gibco). U87MG ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were blocked with anti-mouse CD16/32 (Fc-block, Invitrogen, San Jose, CA, USA) for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11 ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-rabbit IgG Fc secondary antibody (Invitrogen, Cat. No. 31463).
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). MLV P30 protein within the pseudovirus capsid was detected using a rabbit anti-MLV-P30 polyclonal antibody (Origene ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). Beta-actin was used as an internal control.
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bioRxiv - Molecular Biology 2020Quote: ... Absorbance at 570 nm was measured using a spectrophotometer (Multiskan FC Microplate Photometer, Thermo Fisher). A standard curve was generated using standard glycogen solution in the reaction ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Cells were infected with the specified viruses and MOIs in DMEM without FCS (Life Technologies) for 1h at 37.0°C (Cts24 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) for 3 hours before fixation for 15 minutes (3.7% FA and 0.5% triton X-100) ...
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bioRxiv - Molecular Biology 2022Quote: ... and then blocked in PBS supplemented with 20% foetal calf serum (FCS, Thermo Fisher Scientific) and 20% normal goat serum (NGS ...
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bioRxiv - Immunology 2022Quote: ... and were used after freezing in Fetal Calf Serum (FCS) (Invitrogen, Waltham, Massachusetts, United States) containing 10% DMSO (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Absorbance at 405 nm was read on a Multiskan FC plate reader (Thermo Scientific, USA).
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bioRxiv - Biochemistry 2020Quote: ... SKOV3-cells were cultured in McCoy’s 5A Medium supplemented with 10% FCS (Thermo Fisher Scientific). HROC24-cells were cultured in DMEM/Ham’s F12 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 1mM sodium pyruvate and 3.7 g/L NaHCO3 and supplemented with 15% FCS (Gibco, A3160801), 10 mM HEPES (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked with anti-mouse CD16/CD32 (20 µg/ml, clone 93; Invitrogen) for 20min ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (P/S; Thermo Fisher) in a humidified atmosphere at 37°C with 5% CO2 ...
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bioRxiv - Physiology 2019Quote: ... Cells were kept in DMEM supplemented with 10% tetracycline-free Hyclone™ FCS (Fisher Scientific) and the above-mentioned antibiotics.
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice following staining and resuspended in FC buffer containing 7AAD (Life Technologies) or FVS700 (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at 450 nm by a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... supplemented with 4% FCS and 0.75% sodium bicarbonate containing 0.5% low melting agarose (Invitrogen, USA). The overlay was allowed to solidify at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 2mM L-glutamine (all from Life Technologies). HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% foetal calf serum (FCS) and 1mM Sodium Pyruvate (all from Life Technologies). DLD-1 and COLO205 cells were maintained in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Biochemistry 2020Quote: ... The absorbance was measured with a Multiscan™ FC microplate photometer (Thermo Fisher Scientific, USA). Cell viability is presented as the percentage of control (untreated cells) ...