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Citations for Thermo Fisher :
2051 - 2100 of 3296 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Biochemistry 2023Quote: High-titre recombinant baculovirus was obtained using the Bac-to-Bac Baculovirus expression system (Invitrogen) as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant baculovirus was generated via the flashBACTM Baculovirus Expression System using Sf21 cells (Life Technologies), amplified to reach a third passage viral stock ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-titer recombinant baculovirus was obtained using the Bac-to-Bac Baculovirus Expression System (Invitrogen), and used to infect sf9 insect cells to produce D1R proteins as described previously [26] ...
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bioRxiv - Biophysics 2023Quote: ... and subsequently incubated in the same buffer with 1:300 Tom20 Recombinant rabbit antibody (Invitrogen) overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... according to the manufacture protocol and treated with recombinant Rnase-free DNAse I (Thermo Scientific). RNA concentration and purity were determined with NanoDrop 2000 Spectrophotometer (ND-2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
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bioRxiv - Neuroscience 2023Quote: ... The Sf21 insect cells were transfected with recombinant bacmid using Cellfectin reagent (Thermo Fisher Scientific) to obtain recombinant baculovirus ...
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bioRxiv - Immunology 2023Quote: ... the recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as previously detailed (56 ...
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bioRxiv - Genetics 2023Quote: ... Protein concentration of recombinant SIRT5 was subsequently determined by Bradford assay (#23200, Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: Recombinant bacmid production was carried out under manufacturer’s instructions (Bac-to-bac system, Life Technologies). Competent E ...
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bioRxiv - Developmental Biology 2024Quote: Recombinant FGF10 protein was labeled using an Alexa Fluor 488 Microscale Protein Labeling Kit (Invitrogen). To reduce the loss of physiological activity of FGF10 ...
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bioRxiv - Cell Biology 2023Quote: ... the recombinant adenovirus extract was injected into Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher Scientific) and dialyzed in a dialysis buffer at 4 °C with stirring ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... 50 µL of 20 mg/mL recombinant Proteinase K solution (Life Technologies, Catalogue number: AM2548) was then added ...
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bioRxiv - Microbiology 2024Quote: ... equi were prepared using a recombinant plasmid from the TA cloning® kit (Invitrogen, USA). A 10-fold serial dilution of the plasmid (from an initial concentration of 0.5x108 copy number/μL ...
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bioRxiv - Immunology 2024Quote: The recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as reported previously (25 ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Cell Biology 2024Quote: ... in presence of 40 units of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific #10777-019) and 200 units of Reverse Transcriptase per 20 µL of reaction mixture ...
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bioRxiv - Biophysics 2019Quote: ... N-terminal-tagged MBP-TRPA1 under the control of a CMV promoter was expressed in HEK293F cells using the BacMam system (Thermo Fisher). Protein expression was induced at cell density 1.5-2.5 × 106 cells ml- with media supplemented with 5 mM sodium butyrate and 5 µM ruthenium red ...
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bioRxiv - Developmental Biology 2021Quote: ... HA and Myc tagged proteins were visualized on an Amersham Hyperfilm MP (28906845, Cytiva) using SuperSignal WestDuo (34075, Thermo Fisher Scientific).
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3x in PBS with 2% FBS and incubated with secondary antibody (Alexa Fluor 488-conjugated goat anti-mouse IgG) and fluorescently-tagged phalloidin (Molecular Probes), in a humidity chamber for 1hr ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Cell Biology 2019Quote: ... we transfected one 100 mm plate of DLD1 BRCA2-/- cells at 70% of confluence with 10 µg of a plasmid containing human EGFP-MBP-tagged BRCA2 cDNA (corresponding to accession number NM_000059) using TurboFect (Thermo Fisher Scientific), 48h post-transfection the cells were serial diluted and cultured in media containing 1 mg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T cells were grown in 10 cm dishes and transfected with APEX2-tagged mammalian expression plasmids using Lipofectamine 3000 (Thermo Fisher). Cells were washed off the plate 12-15 hours post-transfection with Dulbecco’s PBS and then pelleted at 500x g ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa Kyoto cells were transfected with two guide RNA and one HDR donor plasmid (or 4 guide RNA and two HDR donor plasmids for the EGFP-STAG1 FLAG-mRFP-STAG2 double tagged cell line) using Lipofectamine 2000 (Invitrogen 11668019). Cells were grown for 7-10 days before sorting single cells into 96 well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... RING1Bfl/fl conditional mESCs were transfected with pCAG vectors encoding 2xFlag-HA-tagged mouse wild-type or RING1B I53A/S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: N-terminally MBP-tagged (pTHMT) FUS CHOP fusion type II and CHOP oncogene were expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7–0.9 before induction with 1 mM isopropyl-b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with V5-tagged WNT11 and 24 h post transfection proteins were crosslinked for 30 min with 1 mM DSS (Thermo Fisher) in 1 ml PBS + 1mM MgCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no. A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Genomics 2021Quote: ... MBP-tagged (pTHMT, Peti and Page, 2007) full-length CLAMP protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2021Quote: ... A549 cells were transiently co-transfected with 100 ng in total of an N- or C-terminal LgBiT and SmBiT tagged construct using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher). All possible combinations of the N-terminal and C-terminal-tagged split luciferase protein pairs were tested ...
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bioRxiv - Biochemistry 2021Quote: ... For staining FLAG tagged receptors we used anti-FLAG M1 antibody (1:100) labelled with DyLight594 (Thermo Scientific, Cat. no. 46412) dye in presence of 2 mM CaCl2 ...
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bioRxiv - Neuroscience 2019Quote: pLVX-EF1a-TS-EGFP-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged EGFP cDNA (DNA String byGeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Neuroscience 2019Quote: ... pLVX-EF1a-TS-OPT-FUS-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged codon optimized FUS cDNA (Gene synthesis by GeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Molecular Biology 2020Quote: ... StrepII- and MBP-tagged human PUM1 and PUM2 RD and RBD constructs were expressed in E.coli BL21 (DE3) Star cells (Thermo Fisher Scientific). Cells were grown in LB overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... along with 50 ng and 100 ng of expression plasmids encoding 3xHA-tagged wildtype or mutant mouse PORCN-D as indicated using Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissues were counterstained with wheat germ agglutinin (WGA) tagged with Alexa Fluor™ 647 (#A-31573, ThermoFisher Scientific, Waltham, MA) and DAPI (#H-1500 ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
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bioRxiv - Neuroscience 2020Quote: ... cells were rinsed thrice with PBS and incubated with secondary antibody (donkey anti-goat IgG, tagged with Alexa Fluor 488, Thermo Fisher) at 1:500 dilution for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were transfected with 100ng EGFP to mark cell bodies and 50-100ng of GFP tagged TDP-43 constructs using Lipofectamine 2000 (Invitrogen 52887), as previously described[41 ...