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Citations for Thermo Fisher :
1801 - 1850 of 7266 citations for Human Coronavirus NL63 Nuceloprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and transformed into OneShot TOP10 chemically competent Escherichia coli cells (Invitrogen, Carlsbad, CA) by heat-shock ...
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bioRxiv - Biophysics 2022Quote: ... These proteins were expressed in BL21 Star (DE3) Escherichia coli cells (Life Technologies). The expression and purifications were either in LB or M9 minimal media supplemented with 15NH4Cl with slight modifications as previously described (Conicella et al. ...
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bioRxiv - Microbiology 2019Quote: ... coli was transferred to an Eppendorf tube and centrifuged (Thermo Fisher Scientific, Germany) for 15 mins at a speed of 13000 rpm ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was introduced into Escherichia coli BL21 Star (DE3) (Life Technologies, USA). The resulting expression strain was pre-grown for 16 hours in liquid culture at 37 °C in LB supplemented with 50 μg ml−1 ampicillin ...
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bioRxiv - Microbiology 2019Quote: All cloning steps were carried out in Escherichia coli DH10β (Thermo Fisher Scientific). For protein purification ...
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bioRxiv - Systems Biology 2021Quote: ... the product was transformed into One Shot Stbl3 chemically competent Escherichia coli (Invitrogen). A fraction (150 µL ...
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bioRxiv - Microbiology 2020Quote: ... coli mlaF or mlaB strains were transformed with a pBAD-derived plasmid (Invitrogen) encoding WT mlaF or mlaB genes ...
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bioRxiv - Immunology 2021Quote: ... injections of 1 mg/mL pHrodo-labeled Escherichia coli particles (Thermo Fisher Scientific) resuspended in defRPMI-1640 ...
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bioRxiv - Microbiology 2019Quote: ... coli (10,208 probes per microarray) was cured to only retain data from Affymetrix probes that entirely match within one coding sequence of the REL606 reference genome (NCBI NC_012967.1 ...
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bioRxiv - Biochemistry 2021Quote: ... coli strains DH5α and BL-21-AI were obtained from Invitrogen (Carlsbad, CA). Isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... coli colonies were grown at 37°C shaking in Miller’s lysogeny broth (Invitrogen) until reaching an OD600 of 0.1 when they were induced with a final concentration of 10 mM arabinose to express Csm1-6 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by second strand cDNA synthesis with Eschericcia coli DNA pol I (Invitrogen) and RNAse H (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The pET-28b clone was expressed in Rosetta (DE3) Escherichia coli cells (Invitrogen). The cells were grown to mid log phase ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3)pLysS (Thermo Fisher Scientific). 1-4 liters of cells were grown in Luria-Bertani (LB ...
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bioRxiv - Immunology 2022Quote: ... coli cells containing a baculovirus shuttle vector and helper plasmid (DH10Bac cells; ThermoFisher) to obtain colonies containing recombinant bacmids ...
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bioRxiv - Plant Biology 2022Quote: ... coli clones were first induced with 0.1 mM IPTG (Thermo Fisher Scientific, 15529019) at 16°C for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were performed using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen). Hfx ...
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bioRxiv - Microbiology 2022Quote: ... coli DNA digested with FastDigest HindIII and NotI (Thermo Fisher Scientific, MA, USA) was used as a template in ddPCR reactions optimized for 16S rRNA ...
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bioRxiv - Plant Biology 2022Quote: ... coli BL21 cells using HisPur™ Ni-NTA Spin Columns (Thermo Fisher Scientific). Protein size and purity was assessed by SDS-PAGE (4-12% gradient gel ...
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bioRxiv - Biochemistry 2022Quote: ... coli DH5α competent cells according to the TOPO® Cloning reaction protocol (Invitrogen). Positive transformants were selected on LB-agar-ampicillin (50 μg.mL-1) ...
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bioRxiv - Microbiology 2023Quote: ... overnight Escherichia coli CAN-50 growth in Luria-Bertani (LB, ThermoFisher, Massachusetts, USA) broth equivalent to 109 CFUs was spun down at 16,000 xg ...
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bioRxiv - Bioengineering 2023Quote: ... coli expression were generated using Invitrogen GeneArt web service (Thermo Fisher Scientific, USA). iTerm-PseKNC30 was used for identification of transcriptional terminators ...
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bioRxiv - Biochemistry 2023Quote: ... coli using Strings DNA Fragments Synthesis Service (Thermo Fisher Scientific, Waltham, MA, USA) (Supplementary Data S3) ...
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bioRxiv - Molecular Biology 2023Quote: ... and transformed into One Shot TOP10 Chemically Competent Escherichia coli (ThermoFisher Scientific, www.thermofisher.com). The restriction enzyme-epitope-coding sequence fragment was excised by restriction enzyme digestion ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli and cultured them in LB Miller Broth (Thermo Fisher Scientific, Waltham, MA) with 100 μg/mL Carbenicillin (Gold Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... coli cells and were purified from extracts using glutathione agarose (#16100, Thermo Fisher) as described previously (61) ...
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bioRxiv - Microbiology 2023Quote: ... coli DH5ɑ were washed once in phosphate buffered saline solution (PBS; Thermo Scientific), and pelleted ...
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bioRxiv - Microbiology 2023Quote: ... coli was carried out using Invitrogen TRIzol Reagent (Thermo Fisher Scientific; Cat. # 15596018). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... coli cells was done with DreamTaq polymerase (Thermo Fisher Scientific GmbH, Dreieich, Germany). Phire Plant Direct PCR Master Mix (Thermo Fisher Scientific GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... coli was purified using the GenJET Plasmid Miniprep kit (Thermo Scientific, Waltham, USA). Chromosomal DNA from Hm ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of 6His-CPK3 was induced in Escherichia coli strain BL21-AI (Invitrogen) with 0.2% (m/v ...
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bioRxiv - Microbiology 2024Quote: ... coli strains were performed using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... Final products were electroporated into DH10ß electrocompetent Escherichia coli cells (Thermo Scientific, EC0113) and purified to achieve a highly diverse plasmid library
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... The paraffin-embedded retina was stained with hematoxylin and eosin (H&E) (Thermo Fisher Scientific) to evaluate the cellular structure of the retina ...
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bioRxiv - Neuroscience 2021Quote: ... cortical tissue was dissected from E15.5 embryos and kept on ice in Hibernate-E (GIBCO) supplemented with 2% B27 (GIBCO ...
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bioRxiv - Developmental Biology 2022Quote: ... were maintained in SF900 media (Lif e Technologies) supplemented with 1x Antibiotic-Antimycotic (Life Technologies) at 25°C ...
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bioRxiv - Microbiology 2020Quote: ... were removed from the final pool using a 2% E-gel size-select II (ThermoFisher). Quality of the pool was assessed with the Bioanalyzer DNA 1000 chip (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... The concentration of E/S was measured using the Nanodrop 1000 spectrophotometer (Thermo Fisher Science) and aliquoted before storing at −80°C.
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bioRxiv - Genetics 2019Quote: ... Samples were then pooled together and run on a 4% agarse E-Gel (Life Technologies), and the 140-160 nt length bands were excised and purified using MiniElute Gel Extraction Kit ...
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bioRxiv - Systems Biology 2021Quote: R1/E mouse embryonic stem cells (mESCs) were cultured in DMEM (high glucose, pyruvate, Gibco) supplemented with 16 % FBS (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... Cortices of E-18 Wistar rat embryos were dissociated in trypsin with 0.25% EDTA (Gibco) and counted with a hemocytometer as described previously [32] ...
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bioRxiv - Neuroscience 2021Quote: ... We dissociated cortices of E-18 Wistar rat enzymatically in trypsin with 0.25% EDTA (Gibco), followed by trituration ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...