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Citations for Thermo Fisher :
1701 - 1750 of 7728 citations for Human Coronavirus NL63 Nuceloprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... cells were resuspended in William’s E medium (Thermo Fisher Scientific, Cat No. 12551032) with 5% FBS (Gibco ...
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bioRxiv - Genetics 2024Quote: ... the medium was exchanged for maintenance medium (William’s E Medium (WEM, GIBCO A1217601) supplemented with GIBCO #CM4000).
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bioRxiv - Molecular Biology 2024Quote: ... E-PAP treated RNA was purified using PureLink micro RNA purification kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The amplified DNA was resolved on E-Gel 2% agarose gel (Invitrogen, G401002) on E-Gel Power Snap Electrophoresis device (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: Cortices were dissected from E18 mice and kept in Hibernate-E (Gibco #A1247601) at 4C until dissociation within 1 to 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... J-Lat A1 (gift from E. Verdin) were cultivated in media RPMI (Thermofisher) supplemented as described for the DMEM medium and ...
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bioRxiv - Immunology 2024Quote: ... and gently dissociated by trituration in Hibernate E medium (Life Technologies, cat#A1247601). Neurons were diluted in 5 ml Neuron Growth Medium (Neurobasal media (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... and products separated on 2% EX e-gels containing SYBR Gold II (Invitrogen). qRT-PCR using custom TaqMan primers/probes was performed with TaqMan Fast Advanced Master Mix on a QuantStudio 3 Real-Time PCR System ...
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bioRxiv - Microbiology 2023Quote: ... After two washes with Gibco William’s E Medium (Thermo Fisher Scientific, Cat# A1217601), cells were observed under the EVOS M7000 Imaging System (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Four percent agarose EX E-gels were purchased from Invitrogen (cat. no. G401004). All other chemicals were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2023Quote: ... The PCR product sizes were verified on agarose gel (E-Gel EX; Invitrogen) and quantified with fluorometry (Qubit ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were size-selected with E-Gel EX 2% agarose gels (Life Technologies) and purified by QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Microbiology 2023Quote: ... MHC II (I-A/I-E) (SuperBright645, Thermo Fisher, 1:200,M5/114.15.2), MHC I (H-2Kb/Db ...
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bioRxiv - Bioengineering 2023Quote: ... and 33% complete cholangiocyte culture medium (CCCM: William’s E medium (Gibco, Life Technologies) supplemented with 10 mM nicotinamide (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... and 33% complete cholangiocyte culture medium (CCCM: William’s E medium (Gibco, Life Technologies) supplemented with 10 mM nicotinamide (Sigma-Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was checked on a 1% agarose gel (E-gel EX Invitrogen). 500ng DNA was used from each sample for further analysis ...
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bioRxiv - Physiology 2023Quote: ... H&E sections images were taken using EVOS M7000 imager (Thermo Fisher Scientific) using 20X magnification and analyzed using ImageJ (http://imagej.nih.gov/ij/) ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
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bioRxiv - Neuroscience 2024Quote: ... Embryonic neuronal tissue was dissected and stored in hibernate E medium (ThermoFisher A1247601) on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... the protein construct was transformed into the Escherichia coli BL21 (DE3 Invitrogen) competent cells ...
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bioRxiv - Genetics 2021Quote: ... coli and extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The construction was verified using enzymatic digestion with KpnI and PvuI.
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bioRxiv - Microbiology 2019Quote: ... coli BL21 cells were stained with VybrantTM DiO live cell tracer (ThermoFisher). Cell-labeling solution (5 μl ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were grown in Lucia Bertani (LB) medium (Fisher Scientific) with aeration at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli cultures using PureLink HiPure Plasmid Midiprep Kit (Invitrogen, Cat. No. K210005). To facilitate DNA delivery to ESCs through nucleofection ...
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bioRxiv - Genomics 2020Quote: ... coli (One-Shot MAX Efficiency DH5α-T1 Competent Cells, ThermoFisher, CA# 12297016), followed by plating on LB+Carb plates ...
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bioRxiv - Neuroscience 2019Quote: ... coli BioParticles™ Conjugate (Thermo Fisher Scientific, #P35361, 66.7 ng per well). iMGLs or primary human microglia were incubated with conjugated-synaptosomes (3.44 mg/ml) ...
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bioRxiv - Genetics 2020Quote: ... Escherichia coli strain DH5α was used according to the manufacturer’s recommendations (Invitrogen). Bacterial transformants were selected on LB agar ...
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bioRxiv - Immunology 2019Quote: ... coli TOP10F cells via TOPO cloning (Thermo Fisher Scientific, Waltham, MA, USA). After digesting the purified plasmids containing each individual cloned gene with restriction enzymes ...
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli strains were cultivated in LB medium (Thermo Fisher Scientific, USA) supplemented with appropriate antibiotics ...
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bioRxiv - Microbiology 2019Quote: ... coli One Shot BL21 (DE3) chemically competent cells (Thermo Fisher, Ref: C600003) were transformed with the pACYC184 plasmid according to standard protocol and the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting proteins were expressed in Escherichia coli BL21(DE3) cells (Invitrogen) after induction by 0.5 mM isopropyl β-D-1-thiogalactopyranoside at 18°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The construct was expressed in Escherichia coli strain BL21(DE3) star (Invitrogen) as a fusion protein with a His tag ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 Codon-Plus (DE3-pLys) chemically competent cells (Invitrogen, Carlsbad, CA). Transformed cells with kanamycin and chloramphenicol resistance were cultured at 37 °C with shaking (180 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... coli 70S•PR20 —were collected on a Talos electron microscope (ThermoFisher Scientific) operating at 200 KV and equipped with a K3 direct electron detector (Gatan Inc. ...
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bioRxiv - Plant Biology 2021Quote: ... and introduced into Escherichia coli Mach1 T1R competent cells (Thermo Fisher Scientific). Colonies possessing the fragment of the mutated NbPDS3 gene were identified by colony PCR using primers M13 forward ...
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bioRxiv - Biophysics 2020Quote: ORF1 expression plasmids were transformed into Escherichia coli BL21STAR(DE3) cells (Invitrogen). One-liter cultures supplemented with 50 μg/mL kanamycin were inoculated with 20 mL of an over-night culture and grown at 37°C to an OD600 between 0.5 and 0.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli DH5α cells using a GeneJET Plasmid Miniprep kit (ThermoFisher Scientific K0503). Plasmids were generated by standard restriction digests ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmids were transformed into Escherichia coli BL21(DE3)pLysS cells (Invitrogen), and cells harboring the plasmids were grown at 37°C until the OD600 reached 0.7-1.0 in Luria Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... coli by the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA) and modified or used for full-length genome assembly.
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bioRxiv - Microbiology 2022Quote: ... coli using the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA). Table S3 lists the primers used for the construction of the variant S DNA fragments.
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bioRxiv - Immunology 2019Quote: ... Ifit2 and Ifit3 were expressed in BL21 (DE3) Star Escherichia coli (Invitrogen). Cells were grown to an OD600 of ~1.0 in 2× TY media at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli DH5α or MegaX-DH10B™ T1R Electrocomp™ cells (ThermoFisher Scientific). Transformants were cultured en masse on Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion proteins were expressed in Escherichia coli BL21 Star (DE3) (Invitrogen) or C41 (86 ...
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bioRxiv - Microbiology 2020Quote: ... coli were grown aerobically at 37°C in Luria-Bertani broth (Affymetrix) supplemented with ampicillin (50 ug/mL) ...