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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... followed by 40 cycles at 95 °C (15 s) alternating with 60 °C (1 min) (Applied Biosystems, Carlsbad, CA). Gene-specific products were normalized to a group of 5 housekeeping genes (ACTB ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA sequence corresponding the residues 1-1208 of S-UK was subcloned into the mammalian expression vector pcDNA3.4-TOPO (Invitrogen), which contains a foldon trimerization domain based on phage T4 fibritin followed by a c-myc epitope and a hexa-repeat histidine tag as described previously28 ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (P/S, 10.000 U/ml, Gibco). Jurkat ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 10% fetal bovine serum (AusGeneX, Molendinar, Qld, Australia) and 1% penicillin–streptomycin (P/S; Invitrogen, Carlsbad, CA, USA) with a humidity of 5% CO2 at 37°C.
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bioRxiv - Developmental Biology 2022Quote: ... MK4 cells cultured under standard conditions (DMEM + 10% FBS + 1% P/S) were then transfected with each plasmid using Lipofectamine 3000 per the manufacturer’s protocol (Invitrogen). After 24h ...
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bioRxiv - Neuroscience 2022Quote: ... 1 sec off) with 30 s pause at 40% output using the Fisherbrand Model 120 Sonic Dismembrator (Fisher Scientific). The lysate was clarified by centrifugation at 21000 g ...
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bioRxiv - Immunology 2022Quote: ... Hepa1-6 and 293-AAV cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Meilunbio) supplemented with 1% penicillin/streptomycin (P/S; Invitrogen) and 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... in equal parts supplemented with 1% penicillin-streptomycin (P/S; cat. num. 15140122; Gibco, Thermo Fisher Scientific, Waltham, MA), 1% Insulin-Transferrin-Selenium (ITS+ ...
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bioRxiv - Developmental Biology 2021Quote: ... in equal parts supplemented with 1% penicillin-streptomycin (P/S; cat. num. 15140122; Gibco, Thermo Fisher Scientific, Waltham, MA), 1% Insulin-Transferrin-Selenium (ITS+ ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal bovine serum (FBS) (Summerlin Scientific Products) and 1% penicillin/streptomycin (P/S) (Thermo Fisher Scientific). HCT-116 (Duke Cell Culture Facility-verified ...
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bioRxiv - Neuroscience 2020Quote: ... ECL was added and chemiluminescence was measured with 1 s integration time using Varioskan Flash (Thermo Fisher Scientific, USA).
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bioRxiv - Cancer Biology 2020Quote: ... HCC cell lines and primary hepatocytes were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone) plus with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin-streptomycin (Gibco) in a 37°C incubator with 5% CO2 in air ...
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bioRxiv - Biophysics 2020Quote: ... hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine) (Invitrogen). Cleavage of PED-6 eliminates a self-quenching effect that results in the release of a BODIPY-FL dye ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 and N2a cells were maintained in Dulbecco’s Modified Eagle Medium with 10% Fetal Bovine serum (FBS) and 1% Penicillin/Streptomycin (P/S) (Gibco, Invitrogen). A549 cells (CCL-185 ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were sacrificed by decapitation and dissected brains were first dissociated mechanically and then enzymatically with 0.05% Trypsin-EDTA in DMEM/F-12 supplemented with 1% P/S and (all Gibco) into single cells ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% P/S in tissue culture T-25 flasks coated with 0.1 mg/mL collagen I (A10483-01, Gibco). For experiments ...
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bioRxiv - Microbiology 2023Quote: ... The supplemented-Basal Epithelial Medium (s-BEM) consisted of 3:1 mix of Ham’s F12 (Gibco, Life Technologies, UK) and DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The supplemented-Basal Epithelial Medium (s-BEM) consisted of 3:1 mix of Ham’s F12 (Gibco, Life Technologies, UK) and DMEM (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... FBS (S-FBS-SA-015; Serana) and 1% (v/v) antibiotic penicillin/streptomycin (pen/strep, 10,000 U/mL; Gibco). The cells were cultured at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by a 60-minute incubation with secondary 1:500 streptavidin (SA-HRP, Thermo Fisher Scientific; catalog #: S-911), and a 20-minute incubation with 1:200 tyramide AF555 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 8 days after transfection antibiotic selection was performed using 6 µg mL-1 blasticidin S (Thermo Fisher Scientific, A1113903) to identify cells which had undergone productive editing ...
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bioRxiv - Biochemistry 2023Quote: Murine RAW 264.7 macrophages (ATCC) were cultured in DMEM supplemented with 10% FBS and 1% P/S (Life Technologies). All cells were maintained at 37°C in a humidified incubator containing 5% CO2.
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bioRxiv - Immunology 2023Quote: ... B16 and HEK293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; MA0212, Meilunbio) supplemented with 1% penicillin/streptomycin (P/S; 15140122, Gibco) and 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Physiology 2023Quote: Murine BV2 cell line (ATCC) was cultured in DMEM supplemented with 10% FBS and 1% P/S (Life Technologies) and 1% non-essential amino acids (Euroclone) ...
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bioRxiv - Biophysics 2019Quote: ... The media surrounding the trachea consisted of a solution of fluorescent beads (2 or 0.5 μm diameter; FluoSpheres Invitrogen, 1:250 to 1:500 dilution) in 500μl DMEM/F12 media ...
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bioRxiv - Cancer Biology 2020Quote: ... we added 1:20-1:100 (volume) of 1 mg/ml fluorescent tag (Alexa Fluor® succinimidyl esters of the desired excitation/emission spectrum, Invitrogen™, Molecular Probes®) to the gel premix immediately before polymerization.
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bioRxiv - Neuroscience 2023Quote: ... followed by an incubation in secondary fluorescent antibodies (1:500, Alexa Goat anti-Mouse IgG2b, A-21141, Alexa Goat anti-Rabbit IgG, A32733) and DAPI (Thermo Fisher Scientific; 62248, 1:500) in 0.1x PBS with 0.1% Triton X100 for 1 hour at 22-24°C ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was dissected on ice into 1-mm-thick coronal sections and green fluorescent protein (GFP) was visualized and imaged using an EVOS FL tabletop fluorescent microscope (ThermoFisher Scientific, Waltham, MA). Mice with surgeries that failed to elicit viral overexpression were excluded from the study.
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bioRxiv - Neuroscience 2023Quote: ... Brain slices were stained with green fluorescent Nissl stain (NeuroTrace® 500/525 Green Fluorescent Nissl Stain, Molecular Probes™, Eugene, OR, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The GFP signal was confirmed using a CellDrop Automated Fluorescent Cell Counter (DeNovix) to confirm protein expression prior to imaging on the EVOS M5000 fluorescent imaging system (ThermoFisher, cat no. AMF5000). Cells were incubated 24 h before harvest to analyze protein and mRNA expression.
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovarian cell suspensions were then seeded in 96-well V-bottom plates and incubated with LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Invitrogen, L34962, 1:2000) and Fc Block (BioLegend ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA concentrations were estimated using a fluorimeter and fluorescent DNA-binding dye (Qubit™ dsDNA BR Assay Kit; Thermo Fisher Scientific, USA), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... aliquots of cell suspensions were used to check for equal cell numbers by fluorescent staining (CyQuant Cell Proliferation Assay Kit; Molecular Probes, #C7026). In the setting 2 ...
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bioRxiv - Microbiology 2021Quote: ... DNA quantification in the spent medium of the donor was performed through fluorescent labelling with Quant-iT reagent (Invitrogen Qubit dsDNA HS Assay Kit, Thermo Fisher Scientific). Fluorescence was measured with Infinite M200 PRO (TECAN ...
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... The remaining cells were respectively labelled with aqua-fluorescent reactive dye (dying cells) and calcein violet AM (living cells) using the LIVE/DEAD Violet Viability/Vitality Kit (Molecular Probes, #L34958) under manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2019Quote: ... The quantity of DNA in each digest was measured by fluorescent signal detection using the Quant-iT™ dsDNA High-Sensitivity Assay Kit (Q33232, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... the far-red fluorescent dye Alexa-647 was covalently linked to EdU using the click-iT EdU Imaging Kit (Thermo Fisher, B10184). To detect CDC45 ...
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bioRxiv - Genetics 2023Quote: ... We gated for cells and live cells based on the Near-IR fluorescent reactive dye LIVE/DEADTM (LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, Invitrogen, L10119). Spermatogonia were identified and gated within the diploid (2C ...
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bioRxiv - Molecular Biology 2023Quote: ... Fractions containing the protein were pooled and then incubated in batch with glutathione agarose resin (Thermo Fisher Scientific) for 1 hour at 4°C and washed with wash buffer B (20 mM Tris-HCL pH 8.0 ...
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bioRxiv - Bioengineering 2019Quote: ... followed by 40 cycles at 95° C for 3 s and 60° C for 20 s (7500 Fast Real-Time PCR System; Thermo Fisher Scientific). Taqman probes for Gnat1 (Mm01229120 ...
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bioRxiv - Biophysics 2020Quote: ... The grids were blotted for 4 s or 2 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) at 4°C and 100% humidity ...
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bioRxiv - Biochemistry 2020Quote: Both variants of the recombinant SARS-CoV-2 S protein (OptSpike1 and OptSpike2) were expressed in Expi293F™ and ExpiCHO-S™ cells according to manufacturer’s instructions (ThermoFisher Scientific). For Expi293F™ (ThermoFisher Cat# A14527 ...
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bioRxiv - Neuroscience 2022Quote: HEK293-T cells of low passage (<25) were kept in Dulbecco′s Modified Eagle′s Medium (DMEM, Thermo Fisher Scientific #31966-047) containing 10% fetal calf serum (FCS ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... 40 cycles of 94 °C for 30 s and 60 °C for 30 s with ABI 7700 PCR system (Applied Biosystems, USA). U6 snRNA was used as internal control ...
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bioRxiv - Biochemistry 2023Quote: ... samples were blotted at 100% humidity for 3 s (13°C, 0 s drain time, blot force -3 to +2) with a Vitrobot Mark IV (Thermo Fisher Scientific) and plunged into liquid ethane ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR amplification was performed at 95 °C for 2 minutes and subsequently 40 cycles of 95 °C for 2 s and 60 °C for 20 s on a QuantStudio Real-Time PCR System (Thermo Fisher Scientific). The relative expression of different target RNAs in RNA samples isolated with different methods was analyzed using their Ct values in RT-qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... the sample was incubated for 3 s and blotted for 2 s using the freezing plunger Vitrobot I (Thermo Fisher Scientific, USA). Grids were quickly frozen in liquid ethane cooled by liquid nitrogen and stored in liquid nitrogen until checked ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed 3 times in TBS for 10 min each and incubated for 1 h at RT with a combination of fluorescent dye-labelled secondary antibodies: a Cy3-conjugated goat anti-rabbit (1:200; Invitrogen, Waltham ...