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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were used conjugated with a fluorescent label (Alexa Fluor 488, 546 or 647, 1:1000 Thermo Fisher). Nuclei were stained with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with a solution of red fluorescent streptavidin (Streptavidin, Alexa Fluor™ 555 conjugate, S21381, Thermo Fisher, 1:400) and 3% BSA in PBS for 2h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with anti-green fluorescent protein rabbit IgG primary antibody (1:1000; Molecular Probes, OR, USA) and anti-Tyrosine Hydroxylase (TH ...
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bioRxiv - Neuroscience 2022Quote: ... ECL) or the secondary fluorescent-conjugated (Dylight anti-rabbit 650 and anti-mouse 550 IgG, 1:5000, Fisher Scientific). Membranes were washed within 0.1% Tween 20 PBS ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated in the dark with Alexa Fluor-conjugated fluorescent secondary antibodies (1:500; Invitrogen, Carlsbad, CA). After washing in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... Degradable PEG hydrogel precursors and buffers were prepared as previously described 39,60 and supplemented with 1:60 far-red fluorescent beads (FluoSpheres Carboxylate-Modified Microspheres, 200 nm, Invitrogen). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized in 0.1% Triton X100 and nuclei were stained with 1:10,000 dilution of Hoechst 3342 Fluorescent Stain (Fisher Scientific). Coverslips were then mounted on glass slides using ProLong Gold Antifade Mountant (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: ... Zebrafish eye tissue sections were stained with NeuroTrace 435/455 Blue Fluorescent Nissl Stain (1:50, N21479, Thermo Fisher) for 30 mins at room temperature as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... washed with 0.1M PBS and incubated for 3 hours in a 25:1 solution of 0.1M PBS with 435/455 blue or 530/615 red fluorescent NeuroTrace Nissl stain (Invitrogen). Brain sections were imaged and stitched using a VS200 Virtual Slide fluorescence microscope (Olympus ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice with PBS and stained with fluorescent lipid staining reagents: LipidTOX™ Green (1:500, Invitrogen), for total neutral lipids ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected using a 1:10,000 dilution of fluorescent IRDye 800CW goat anti-mouse IgG (925-32210; Thermofisher) or IRDye 680RD Goat anti-Mouse IgG Secondary Antibody (925-68070 ...
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bioRxiv - Microbiology 2019Quote: ... The amplicons were pooled in equimolar concentrations using SequalPrep plate normalization kit (Invitrogen) and then purified with AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2019Quote: Amplicons were cleaned and quantified using a SequalPrep normalization plate kit (Invitrogen, USA), and a Qubit dsDNA High Sensitivity Assay kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Samples were normalized using SequalPrep Normalization Plate (96) Kit (Invitrogen, Maryland, MD, USA) and pooled using 5 μl volume of each ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from cells trypsinized from plates using the MirVana kit (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PCR amplicons were normalized (SequalPrep normalizatin plate kit from Thermo Fisher Scientific), pooled and quantified (KAPA library quantification kit from KAPA Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were normalized using SequalPrep Normalization Plate (96) Kit (Invitrogen, Maryland, MD, USA) and pooled using a 5 µl volume for each sample ...
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bioRxiv - Microbiology 2019Quote: ... Triplicate reactions were combined and purified using the SequalPrep Normalization Plate Kit (Invitrogen) according to manufacturer’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... All PCR products were normalized using the SequalPrep Normalization Plate kit (Invitrogen, USA) and sequenced on a MiSeq Next Generation sequencing platform (2x300 bp ...
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bioRxiv - Microbiology 2023Quote: ... Barcoded samples were purified and normalized over a SequalPrep Normalization Plate Kit (Invitrogen) using a Biomek NXP Span-8 pipetting robot (Beckman Coulter) ...
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bioRxiv - Genetics 2020Quote: ... Primer pairs producing a single product band of expected size during PCR amplification were shortlisted for fluorescent dye labelling (forward primers) with one of four fluorescent dyes (6-FAM, VIC, NED, or PET, Invitrogen, South Korea) to perform fragment analysis using Applied Biosystems 3130 Genetic Analyser ...
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bioRxiv - Biochemistry 2020Quote: ... and then loaded with a fluorescent NO indicator DAF-FM diacetate (SKM423741, Sekisui Medical) or a fluorescent ROS indicator CellROX (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The neutrophils were incubated at 37 °C in 5% CO2 for 1 hour before adding Syto Green (cell-permeable) and Sytox orange (cell-impermeable) fluorescent dye mixture and visualized by fluorescent microscopy (EVOS Fluorescence microscope, Thermo Scientific, USA). The two dye images were merged using ImageJ software.
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bioRxiv - Developmental Biology 2022Quote: ... RNAi Inc.) or 4 μM red fluorescent non-targeting siRNA (BLOCK-iT™ Alexa Fluor® Red Fluorescent Control; Thermo Fisher Scientific) was prepared as siRNA solution ...
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bioRxiv - Biophysics 2023Quote: Cells expressing an endogenous fluorescent tag on alpha-actinin were imaged using the integrated fluorescent light microscope (iFLM) (ThermoFisher Aquilos2 cryoFIB-SEM) to identify cells with ordered sarcomeric arrangement ...
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Biophysics 2021Quote: ... Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Cryo- EM datasets were collected on a Titan Krios G2 electron microscope operated at 300 kV equipped with a GIF (Gatan ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were conducted at 95°C for 10 min followed by 40 cycles at 95°C for 15 s and 60°C for 30 s on a real-time quantitative PCR system (7900HT Fast Real-Time PCR; Applied Biosystems). Each real-time PCR reaction was performed in triplicate ...
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bioRxiv - Microbiology 2021Quote: ... ACE2-S-tag expression was detected by using a mouse anti-S-tag monoclonal antibody 6.2 (Invitrogen, Cat. No. MA1-981), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were incubated with 1.5 mg/mL EZ-Link™ Sulfo-NHS-S-S-Biotin (#21331; Thermo Fisher Scientific, USA) in PBS for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 2X Phusion Master Mix (Cat. No. F531L), and Dulbecco′s Modified-Eagle′s Medium (DMEM, Cat. No. 11995-065) were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2022Quote: ... A 3-μL aliquot of the purified virions was applied to the grids and after 30 s blotted for 4.5 s and plunge-frozen in liquid ethane with a vitrification apparatus (Vitrobot Mark IV, Thermo Fisher Scientific) equilibrated to 6 °C and 100% relative humidity ...
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bioRxiv - Plant Biology 2019Quote: ... 40 cycles of 95°C for 3 s and 60°C for 30 s was run in a StepOne Real-Time PCR machine (Applied Biosystems). The quality of the graphs ...
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bioRxiv - Neuroscience 2021Quote: ... The anterior part of the tongue was stimulated with different tastants (applied for 30 s and then rinsed with water for 40 s) with a constant flow pump (Fisher Scientific). Stimuli consisted of (in mM) ...
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bioRxiv - Microbiology 2022Quote: ... MLE-12 cells were maintained in Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham (DMEM/F-12; Gibco, US) medium containing 2% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: BHK-T7 cells expressing the T7 polymerase (46) were maintained in Dulbecco′s modified Eagle′s medium (DMEM) (Invitrogen life technologies) supplemented with 10% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Immunology 2022Quote: The pcDNA3.1 plasmids encoding S and S-Fc were transfected into CHO cells using PEI MAX 40000 (Fisher Scientific, Cat# NC1038561). Stable cell lines were selected and maintained under G418 (1 mg/ml) ...
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bioRxiv - Bioengineering 2023Quote: HEK293 and HEK293T from the American Type Culture Collection (ATCC) were cultured in Dulbecco′s modified Eagle′s medium (DMEM; Life Technologies) with 10% FCS (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... embedded with 0.5 μm diameter fluorescent beads (ThermoFisher Scientific), were prepared ...
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bioRxiv - Cancer Biology 2021Quote: ... After incubation with a fluorescent-labelled secondary antibody (Invitrogen; Jackson Immunoresearch ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... and STYO BC Green Fluorescent Nucleic Acid Stain (Invitrogen) at 1:750 dilution each for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... calibrated with a standard fluorescent beads (LinearFlow, Thermo Fisher)
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bioRxiv - Cell Biology 2020Quote: ... followed by 45 min with fluorescent phalloidin (Molecular Probes) in PSG ...
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bioRxiv - Immunology 2021Quote: ... fluorescent microscopy (EVOS™ M5000 Cell Imaging System, Invitrogen), infectious TCID50 titer measurement 39 ...
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bioRxiv - Microbiology 2019Quote: ... uninfected macrophages using MitoSox fluorescent dye (Thermo Fisher Scientific), at 5µM for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... 500 nm diameter fluorescent latex beads (Thermo Fisher Scientific) are PEG-coated following previously published protocols62 ...
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bioRxiv - Developmental Biology 2020Quote: ... species-appropriate fluorescent antibodies were applied (AlexaFluor, ThermoFisher Scientific), and DAPI was used to counterstain nuclei ...
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bioRxiv - Neuroscience 2022Quote: A fluorescent tracer (10% Dextran AlexaFluor 594, ThermoFisher Scientific) was injected with a stereotaxic frame (KOPF ...
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bioRxiv - Neuroscience 2021Quote: ... Appropriate fluorescent secondary Abs were used (Alexa Fluor, Invitrogen).