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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Purification and normalization of amplicons were performed using a SequalPrep plate kit (Invitrogen, USA), and the DNA concentration was measured with a Qubit® Fluorometer ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned and normalized using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific), then quantified and pooled equimolarly according to Kapa Biosystems Library qPCR MasterMix (ROX Low ...
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bioRxiv - Microbiology 2023Quote: ... Barcoded amplicons were again purified and normalized with the SequalPrep Normalization Plate Kit (Invitrogen), pooled ...
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bioRxiv - Microbiology 2023Quote: ... Normalization was performed using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, Darmstadt, Germany) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were normalized using a SequalPrep Normalization Plate (96) Kit (A1051001; Invitrogen, Frederick, MD). Paired-end sequencing was performed on an Illumina MiSeq26 at the Nebraska Food for Health Center ...
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bioRxiv - Microbiology 2024Quote: ... and mock community were normalized using SequalPrep Normalization Plate Kit (Invitrogen, Carlsbad, CA, USA) and pooled into equal volumes ...
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bioRxiv - Synthetic Biology 2021Quote: ExpiCHO-S cells (6E6cells/mL) were transfected with 1ug/mL of Spike protein DNA22 using the ExpiCHO™ Expression System Kit (Gibco #A29133) and shaken at 37°C and 8% CO2 in a 50mL culture ...
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bioRxiv - Biophysics 2021Quote: Expression SΔTM trimers was performed by transfection of ExpiCHO-S™ cells with the plasmids described above using the ExpiFectamine™ CHO transfection kit (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: Biotinylation of HBs-S and HBs-L was performed using the Antibody Biotinylation Kit for IP (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... supplemented with MSC Growth Kit Low Serum (ATCC, PCS-500-040, VA, USA) and 0.1% v/v penicillin-streptomycin (P/S, Invitrogen, 15140, Barcelona, Spain). Medium was replaced every 2-3 days ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured in a Dulbecco’s Modified Eagle’s medium (DMEM) containing 10% FBS and transfected by pLV-Spike using a calcium phosphate kit (ThermoFisher, Waltham, MA, USA). The control virus with VSV-G as the tropism and expression of mCherry was generated by co-transfection of pLV-mCherry and pMD2.G vector (Addgene ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... The plasmid expression plasmid carrying each SARS-CoV-2-associated protein was transiently transfected into ExpiCHO-S cells by using the ExpiFectamine™ CHO transfection kit according to the manufacturer’s instruction (Thermo Scientific, A29129). After transfection ...
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bioRxiv - Neuroscience 2022Quote: ... > 8.0 were further processed with the Affymetrix WT Plus kit and hybridized to Clariom S mouse arrays as described by the manufacturer (Thermo Fisher, Germany). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... according to manufacturer’s instructions and 200 ng RNA was used to make cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems #4368814). The cDNA was diluted 1:5 and a qPCR reaction mixture composed of the diluted cDNA ...
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bioRxiv - Developmental Biology 2023Quote: Cells in S-phase were detected by EdU-incorporation using the Click-ItTM EdU Alexa FluorTM 647 Imaging Kit (C10340, Thermo Fisher Scientific) following the supplier instructions ...
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bioRxiv - Immunology 2023Quote: ... The AF-03 and AF03-NL plasmid were transfected into ExpiCHO-S cells using the ExpiFectamine™ CHO Transfection Kit (Gibco, A29129) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... were transfected with the plasmid encoding the stabilized SARS-CoV-2 soluble S protein with the Expifectamine kit (GIBCO, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of Aβ1-40 and Aβ1-42 in the s-extracts was determined by commercially available ELISA kits (ThermoFisher Scientific: KHB3481 and KHB3544). Aβ1-42 was undetectable in the Aβ negative sample ...
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bioRxiv - Microbiology 2021Quote: ... washed with phosphate buffered saline (PBS) and the resin digested with His6-GST-HRV3C protease (Thermo Fisher Scientific) 16-18 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... With the following exceptions: the nitrocellulose-coated coverslips were incubated with protein or with anti-GST antibody (Invitrogen) for 4 min ...
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bioRxiv - Cell Biology 2022Quote: ... GST-PLK-2 was expressed in insect Sf9 cells using the standard Bac-to-Bac system (Life Technologies) and then purified using glutathione Sepharose (GE Life Sciences).
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bioRxiv - Biochemistry 2023Quote: Mice were intraperitoneally injected with 10µg of purified GST-SLAPTAG or GFP in aluminum hydroxide (Imject Alum ThermoFisher). Two and four weeks later ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Labelling of 647His6-GST-CENP-E was performed according to the manufacturer’s instructions with the AlexaFluor647 (A20173A, Invitrogen). Degree of labelling estimated as 1:3.
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bioRxiv - Neuroscience 2022Quote: ... Brain sections that contained the mPFC were stained with a fluorescent Nissl stain (NeuroTrace 530/615 Red Fluorescent Nissl Stain, Thermo Fisher, Waltham, MA, USA), and expression of the viral construct was evaluated by visualizing GFP with a fluorescent microscope (MVX10 ...
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bioRxiv - Biochemistry 2023Quote: ELISA plates (Maxisorp, 96 well plate, Invitrogen) were coated with 100 μL of 7.5 ng/μL Streptactin (IBA ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates (Nunc Maxisorp Immuno Plate) were coated overnight at 4 °C with 2.0 µg of the recombinant P ...
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bioRxiv - Developmental Biology 2024Quote: ... Feeder-depleted serum cultured ESCs were plated on gelatin-coated plates at 10,000 cells/cm2 (90,000 cells per well in a 6-well plate) in advanced N2B27 defined media composed of a 1:1 mix of 1:1 DMEM/F12 (Invitrogen, 12634-010) and Neurobasal medium (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154, Fisher Scientific, Waltham, MA, USA). Following purification of total RNA ...
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bioRxiv - Developmental Biology 2020Quote: ... HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154, Fisher Scientific, Waltham, MA, USA). Following purification of total RNA ...
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bioRxiv - Microbiology 2019Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 20 s and was run on QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). Provided that the efficiencies of the reactions were between 90% and 110% ...
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bioRxiv - Molecular Biology 2023Quote: ... and NCI-H520 (ATCC® HTB-182™) cells were maintained in Dulbecco′s Modified Eagle′s Medium (DMEM) high glucose pyruvate medium (Gibco, #10569010), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 3.5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Neuroscience 2024Quote: ... (10 µl per mg tissue) After homogenizing with a Sonic Dismembrator (8 s on, 15 s off, three cycles in total, Fisher Scientific Model FB120), the mixture was centrifuged at 20,000 g (20 min ...
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bioRxiv - Immunology 2020Quote: ... Plates were developed using 1-Step Ultra TMB-ELISA (34028, Thermo Fisher) for one to two minutes after which the reaction was stopped with 1M H2SO4 ...
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bioRxiv - Neuroscience 2021Quote: ... on six-well plates coated with 1:100 Geltrex (Life technologies; A1413302). Media was changed every 48 hours and passaged when 70-80% confluent with HBSS and Versene (Gibco ...
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bioRxiv - Immunology 2020Quote: ... The plate was blocked with PBS+1%BSA (Fisher Scientific, PA, USA)+0.1%Tween20™ (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... HepG2 cells (1×105) were seeded in 24-well plates (ThermoFisher Scientific). Two days after the second silencing (Mock or siTANGO2) ...
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bioRxiv - Microbiology 2023Quote: ... Plates were developed using 150μl 1-Step ABTS substrate (ThermoFisher, Prod#37615). Following a brief incubation at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Plates were developed using 150μl 1-Step ABTS substrate (ThermoFisher, Prod#37615). Following a brief incubation at room temperature ...
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bioRxiv - Immunology 2024Quote: ... plates were developed using TMB 1-Component Peroxidase Substrate (Thermo Fisher Scientific), followed by reaction termination using the TMB stop solution (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 12-well plates were coated overnight with 1% GelTrex (Thermo Fisher Scientific) diluted in H2O ...
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Neuroscience 2019Quote: ... slices were incubated overnight at 16°C in PBS with Alexa 633-conjugated streptavidin (final concentration 1 μg/mL, catalogue no. S-21375; Invitrogen). Slices were cryopreserved in 30% sucrose containing PBS and then re-sectioned at 100-150 μm thickness using a sliding freezing microtome (HM430 ...
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bioRxiv - Synthetic Biology 2020Quote: ... were maintained in Dulbecco’s modified eagle medium supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin (ThermoFisher). Cells were maintained in a 37°C incubator with 5% CO2 and passaged once ∼80% confluent.
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bioRxiv - Biophysics 2021Quote: ... the grids were blotted for 2 s with a blot force of 1 in a Vitrobot Mark IV (Thermo Fisher) and plunge-frozen in liquid ethane ...
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bioRxiv - Biochemistry 2021Quote: ... 2.25 mg/mL S protein and S protein-ACE2 complex (1:2.3 S protein trimer:ACE2 molar ratio) samples were vitrified using a Vitrobot Mark IV (Thermo Fisher Scientific) plunge freezing device ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5-μm frozen sections of uterine myometrium embedded in OCT compound were obtained using Leica CM1510 S cryostat and immunostained for phosphorylated myosin light chain kinase (1:200 rabbit anti-mouse phosphomyosin light chain kinase, Invitrogen) and imaged with the Zeiss Axioskop 40 microscope ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) penicillin [10,000 U/mL]–streptomycin [10,000 μg/mL] (p/s; GIBCO, Life Technologies™, New York, USA), and 1% non-essential amino acids in 75 cm2 culture flasks (NUNC™ ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) penicillin [10,000 U/mL]–streptomycin [10,000 μg/mL] (p/s; GIBCO, Life Technologies™, New York, USA), and 1% non-essential amino acids in 75 cm2 culture flasks (NUNC™ ...