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Citations for Thermo Fisher :
1751 - 1800 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was run on 2% agarose gels and bands were purified using the GeneJET gel purification kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products and products of restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). DNA sequences were analyzed by DNA sequence service of Fasmac Co. ...
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bioRxiv - Cancer Biology 2022Quote: ... The same protein samples were loaded onto a 15% SDS polyacrylamide gel and then stained with SYPRO Ruby Protein Gel Stain (Thermo Fisher Scientific) to confirm an equivalent sample loading in each lane.
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplicons of these four primer pairs were then purified from 1% agarose gel using Qiagen Gel Extraction kit and concentrations were measured with Qbit High Sensitivity DNA kit (Thermo Fisher Scientific). Each amplicon was then diluted to 1×109 molecules per µl ...
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bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PCR products were resolved by gel electrophoresis (1% agarose) in the presence of a DNA gel stain (SYBR Safe, Invitrogen, Carlsbad, CA). Bands were visualized on an imaging station (Biospectrum 500 ...
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bioRxiv - Genetics 2021Quote: ... The first step was to resolve the products of a standard PCR on a 3% agarose gel in Tris-acetate-EDTA gel stained with SYBR safe (Thermo Fisher Scientific), to assess the correct product size and presence of only one product ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated on either NuPAGE Novex 10% polyacrylamide Bis– Tris gels with 1XNuPAGE MOPS SDS buffer or 6% Tris-glycine gels with 1XTris-glycine running buffer (all Thermo Fisher Scientific), and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2020Quote: ... and 25 μg of protein per sample were resolved by polyacrylamide gel electrophoresis on 10% Tris-Glycine gels (Catalogue Number XP00105BOX) (Thermo Fisher Scientific) and immobilized onto nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots of 13 μl were loaded on 3% agarose gels (Invitrogen UltraPure Agarose 1000, 1X TAE buffer, Invitrogen SYBR Safe Gel Stain) and electrophoresed for 30 minutes at 100 volts ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products and products of restriction digests were routinely purified using preparative agarose gel electrophoresis followed by DNA isolation with the GeneJET gel extraction kit (Thermo Fisher Scientific). Restriction endonucleases were purchased from Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Libraries were PCR-amplified for 12-15 cycles and size selected for fragments (200-400 bp for ChIP-seq, 200-500 for RNA-seq) by gel extraction (10% TBE gels, Life Technologies EC62752BOX). RNA-seq and ChIP-seq librairies were single-end sequenced for 76 cycles on an Illumina HiSeq 4000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... DNA fragments of around 250-300 bp were size-selected on an E-Gel™ SizeSelect™ II Agarose Gels 2% (Thermofisher) as an intermediate step of the library preparation ...
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bioRxiv - Cell Biology 2021Quote: Proteins were separated by SDS-PAGE (8 % gels) and then transferred to a nitrocellulose membrane with an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 % and incubated over-night with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Vector fragments were generated by restriction of plasmids and the correct bands were extracted from gel using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Samples were boiled at 95°C for 5min and subsequently separated by polyacrylamide gel electrophoresis on a 4-12% pre-cast NuPage Bis-Tris protein gel (Thermo Fisher Scientific). Finally ...
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bioRxiv - Biochemistry 2021Quote: Western blotting was performed using mini gel tank system (Life Biotechnologies) with 4-12% gradient Bis-Tris gels (NuPage, Invitrogen cat. #NP0335) and 0.45 μm pore size nitrocellulose membranes (BioRad cat ...
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bioRxiv - Microbiology 2021Quote: ... where the strains usually meet and 20 samples from the meeting point on B medium plate with/without added DNA were resuspended in 700 µl of 0.9 % NaCl instead of Gel Solubilization Buffer from the PureLink® Quick Gel Extraction Kit (Thermo Scientific, ZDA) to prevent damaging viable cells ...
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bioRxiv - Molecular Biology 2020Quote: ... The distinct protein bands were excised and were subjected to in-gel trypsin digestion using In-Gel Tryptic Digestion Kit (Thermo Scientific, USA). The recovered peptides in digestion buffer were dried under vacuum and dissolved in 0.1% Trifluoroacetic acid (TFA ...
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bioRxiv - Cancer Biology 2020Quote: ... The gels were transferred to a nitrocellulose membrane using the iBlot Gel Transfer at P3 for 6 minutes (Thermo Fisher, cat#IB23001) and then blocked for 1 h at room temperature in Odyssey blocking buffer (LICOR Biosciences ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were boiled run on SDS-PAGE gels (10% acrylamide) and subsequently transferred to a nitrocellulose membrane using an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 % ...
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bioRxiv - Cell Biology 2022Quote: ... Mini Protein Gels and the gel run at 100 V for 75 minutes in NuPAGE MOPS SDS Running Buffer (all Thermo Fisher Scientific). The proteins were transferred onto an Immobilon-fl polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... The first round PCR sub-fragments were purified by extraction from an agarose gel using a GeneJET Gel Extraction Kit (Thermo Scientific™, ThermoFisher). A total mass of 10-15 ng of sub-fragments at a 1:1 molar ratio were then used as templates for OL-PCR using the forward and reverse outer primers ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μg aliquots of total protein were transferred to hand-cast polyacrylamide gel or to Novex NuPAGE 4-12 % Bis-Tris Precast Gels (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2022Quote: ... PCR products were run out on a 1% TBE (Tris-Borate-EDTA) agarose gel stained with SYBR Safe DNA gel stain (Invitrogen Life Technologies) and visualized in a blue-light transilluminator ...
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bioRxiv - Physiology 2024Quote: Titin isoforms were separated on a 1% w/v agarose gel and stained with SYPRO Ruby protein gel stain (Invitrogen, ThermoFisher Scientific) as previously described.30 All samples were measured in triplicate and the average of triplicate measurements per sample was shown.
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Loss of the mitochondrial citrate carrier, Slc25a1/CIC disrupts embryogenesis via 2-HydroxyglutaratebioRxiv - Developmental Biology 2024Quote: ... Equal amount of protein lysate was loaded and separated in an SDS-polyacrylamide gel electrophoresis using Novex™ 4-20% Tris-Glycine Mini Gel (Invitrogen, XP04200PK2) and transferred to PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) in 8% polyacrylamide gel together with PageRuler protein ladder (Thermo Fisher Scientific) and transferred onto polyvinylidene difluoride membrane (PVDF) ...
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bioRxiv - Genetics 2024Quote: The quality of the purified DNA was assessed through gel electrophoresis on a 0.8% agarose gel and a Thermo Scientific™ NanoDrop Lite spectrophotometer (Thermo Scientific, UK). The DNA in each sample was quantified with a Qubit 4 Fluorometer (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... The final eluate of 2 rows was used for SDS gel electrophoresis under reducing conditions on a 4–12% bis-tris polyacrylamide gel (Life Technologies, NP0321BOX), which was blotted onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Confirmation of PCR reaction was carried out on a 1% w/v agarose in 1x TBE (89 mM tris, 89 mM boric acid, 2 mM EDTA) gel containing 1x SYBR™ Safe DNA gel stain (Invitrogen™) using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... 500 ng of the PCR reaction 2 amplicons were gel purified on a Size Select II E-Gel (ThermoFisher Scientific Cat#G661012) for approximately 13 minutes to extract the 260bp amplicon band of interest ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products and products of restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). DNA sequences were analyzed by DNA sequence service of Fasmac Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate Bis-Tris polyacrylamide gel electrophoresis (SDS-PAGE) (NuPAGE™ 4 to 12%, Bis-Tris precast polyacrylamide gel, Invitrogen # NP0323BOX) and afterward transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell-free synthesized protein was visualized by Coomassie blue staining after protein gel electrophoresis using pre-casted 4-12% Bis-Tris gradient gel (Invitrogen, Waltham, MA). Purified protein concentration was measured by Bradford Assay.
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bioRxiv - Cell Biology 2023Quote: ... 15-20 µg of NE was heated in SDS loading buffer at 95C for 5min and loaded on to an 8-12% acrylamide gel or a tris-acrylamide NuPAGE gradient gel (Thermo Fisher Scientific) and separated by electrophoresis ...
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bioRxiv - Genetics 2023Quote: ... PCR products were then run on an agarose gel and the desired band was excised and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific). Purified PCR products were prepared for sequencing using the standard Illumina DNA Prep protocol and reagents (Illumina DNA Prep (M) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by 8% acrylamide SDS-PAGE gels and then transferred to a nitrocellulose membrane with an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies ...
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bioRxiv - Systems Biology 2023Quote: ... amplified libraries were size selected for ∼270 bp (mono-nucleosomes + ∼125 bp NextFlex adapter) using 2% E-Gel Size Select Agarose Gels (Thermo Fisher Scientific) and again amplified as described above for 9 cycles ...
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bioRxiv - Cell Biology 2023Quote: For the SILAC APEX experiment (HCT116-APEX vs HCT116-unmodified) proteins were separated on gel (NuPAGE Novex 4-12% Bis-Tris gel, Life Technologies, UK), in NuPAGE buffer (MES ...
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bioRxiv - Genetics 2023Quote: ... 5 µg of small RNA fraction was run on 15% TBE/Urea gel using Owl™ S4S sequencing gel system (Thermo Scientific) and small RNAs of 20- 35 nt were gel-purified for library construction (input) ...
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bioRxiv - Neuroscience 2023Quote: ... Gel electrophoresis was performed with the Novex NuPage SDS-Page Gel System by running 10 μg of total protein on pre-cast SDS-PAGE gels (Life Technologies, NPO335). Gels were transferred onto PVDF membranes (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on NuPage™ Novex™ 4-12% Bis-Tris protein gels in MES buffer or on E-PAGE 8% 48-well gels (ThermoFisher). For native running conditions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The PCR products of each gene were then separated on 2% agarose gels containing SYBR® Safe DNA Gel Stain (ThermoFisher Scientific), at 100V ...
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bioRxiv - Bioengineering 2024Quote: PCR products were run on a 1% agarose gel and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, cat.: FERK0692). Sanger sequencing of respective PCR samples was performed using the reverse primer ...
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bioRxiv - Genetics 2024Quote: ... 500 ng of the second PCR reaction amplicons were gel purified on a Size Select II E-Gel (ThermoFisher Scientific Cat#G661012) for approximately 13 minutes to extract the approximately 250-bp amplicon band ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μl of this mixture was loaded into the lanes of a precast 4-12% gradient polyacrylamide gel (4–12% Bolt Bis-Tris Plus Gels, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were separated by SDS-PAGE ((4-20%; Criterion XT precast gel) with 1× MOPS SDS gel running buffer (NP0001, Invitrogen, Life Technologies Corp). Following electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... SDS-PAGE was performed using a 7.5% gel (Oriental Instruments Co., Ltd.; Sagamihara, Japan) or 4–20% gel (Invitrogen, Carlsbad, CA, USA) at 100 V for 2 hours ...