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Citations for Thermo Fisher :
1601 - 1650 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Genomics 2019Quote: ... PCR products from PAT assay and TVN PCR were run on same native TBE gels and stained with SYBR® Gold Nucleic Acid Gel Stain (Invitrogen) for analysis.
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bioRxiv - Genetics 2019Quote: ... Enriched and shotgun libraries were then pooled separately and size selected on an E-Gel SizeSelect 2% agarose gel (Thermo Fisher). Enriched libraries were then sequenced on an Illumina MiSeq ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We first PCR amplified J and ran the product on a 1% agarose gel prepared with SYBR™ Safe DNA Gel stain (Invitrogen). The band was excised and the Wizard® SV Gel and PCR Clean-up System (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... prior to SDS-PAGE gel electrophoresis on 1.5 mm NuPAGE Novex 4-12 % Bis-Tris Protein Gels (Thermo Fisher Scientific, USA), using Mini Trans-Blot Cell system (BioRad ...
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bioRxiv - Genetics 2019Quote: ... the PCR products in the range from 350 bp to 10 kb were cut out and extracted from the gel (GeneJet Gel Extraction Kit, Thermo Scientific).
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bioRxiv - Evolutionary Biology 2021Quote: ... We selected libraries within the size range of 450-500 bp using a 2% E-gel on an E-Gel Electrophoresis System (Invitrogen, Canada), and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions were resolved in an agarose gel and the amplicon extracted using the PureLink Quick Gel Extraction and PCR purification Combo Kit (ThermoFisher Scientific). Amplicons were sequenced at the LGT Core at Texas A&M using the same primers as for amplification.
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bioRxiv - Genomics 2021Quote: ... DNA fragments between 200 and 1000bp were isolated from the 4C libraries by gel excision after running the 4C libraries on a 4-20% gradient TBE gel (Thermo Scientific). The gel slices were shredded and macerated in Tris EDTA buffer at 37°C overnight and DNA was precipitated using ethanol ...
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bioRxiv - Cancer Biology 2021Quote: Cultured cells were collected and lysed with RIPA buffer and a total of 20 μg protein was separated by SDS-polyacrylamide gel electrophoresis using 4-12% NuPAGE Bis-Tris gels (ThermoFisher Scientific), blotted to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... Deglycosylation on the standard proteins was analyzed by gel electrophoresis on 15% SDS-PAGE gels and the gels stained by Coomassie Brilliant Blue R-250 Dye (Cat. 20278, Thermo Scientific). The optimal conditions were applied to study limited deglycosylation on LLC-MK2 cellular glycoproteins upon induction of ER stress ...
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bioRxiv - Microbiology 2020Quote: ... The presence of SARS-CoV-2 RNA was confirmed through UV visualization of a PCR product of the expected length on a 2% agarose gel stained with Sybr-Safe DNA Gel Stain (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
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bioRxiv - Microbiology 2020Quote: ... The presence of glycoproteins was examined by staining the gels with SYPRO Ruby protein gel stain according to the manufacturer’s instructions (Thermo Fisher Scientific). Stained LPS and protein bands were visualized under UV light using an Azure c300 gel imaging system (Azure Biosystems).
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bioRxiv - Microbiology 2021Quote: ... Extracted DNA was visualized on a 1% agarose gel using Gel Red nucleic acid stain and quantified using Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and the NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... 10uL of the protein lysate was loaded into a bis-tris protein gel (4-12% Invitrogen Bolt Bis-Tris mini gel), ran at 150V for 35min then transferred to PVDF membrane using the iBlot2 system ...
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bioRxiv - Microbiology 2021Quote: Proteins extracted from cell lysates were resolved by SDS-polyacrylamide gel electrophoresis on 4-12% NuPAGE Bis-Tris Gel (Life Technologies) with MOPS running buffer and semi-dry transferred to a nitrocellulose membrane with Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR product visualization was performed using agarose gel electrophoresis with appropriate agarose concentration and SYBR® Safe DNA Gel Stain (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... After protease exposure the samples were analysed by reducing and denaturing polyacrylamide gel electrophoresis on 4-12% bis-tris gels (Life Technologies). Protein bands were developed with Simply Blue Safe Stain (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then separated using gel electrophoresis using 4-12% Bis-Tris Novex Midi 20 well gels (Invitrogen, Cat. No. #WG1402BOX) with 1X MES Buffer (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... transcript size and integrity of all RNA constructs were examined under denaturing conditions with 7M Urea-PAGE gels stained with SYBR™ Safe DNA Gel Stain (Invitrogen), with RNA Century™-Plus Markers 0.1–1 kb (Ambion) ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on a 10% SDS-PAGE gel and the gel contents were transferred to a nitrocellulose membrane (ThermoFisher, 88018). Membrane was blocked for 30 min in TBS-T (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The mixtures were then analyzed by electrophoresis through a urea-15% polyacrylamide gel (Novex TBE-Urea gel 15%, Thermo Fisher Scientific). To acquire the fluorescent signals ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were loaded on 10% polyacrylamide gels or were run on NuPage Novex 4–12% Bis-Tris gels (Life Technologies) and blotted onto nitrocellulose membranes using the iBlot device (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... Second dimension analysis was performed by solubilizing BN-PAGE gel slices in 2x Laemmli buffer (Laemmli, 1970) and loading into precast 2D gels (Life Technologies).
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bioRxiv - Physiology 2022Quote: The amplified fragments were cut out of their gel bands and purified using a PureLink Quick Gel Extract Kit (Thermo Fisher), cloned into a plasmid pCR 2.1-TOPO TA vector (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... concentration of 1.5% in Tris/Borate/EDTA (TBE) buffer and gel stained with SYBR® Safe DNA Gel Stain (Invitrogen®). GEL DOC 1000 image processor (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Both the insert (MCR-MOR) and vector fragments were gel purified using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific). The gel-purified PCR products were assembled together using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates of HAP1 and HeLa cells were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% gradient precast polyacrylamide gels (Life Technologies). The proteins separated by SDS-PAGE were transferred onto a nitrocellulose membrane using a Mini-Protean® Tetra Cell device ...
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bioRxiv - Neuroscience 2021Quote: ... The designated samples were then subjected to one-dimensional gel electrophoresis (1DE) employing precast NuPAGE 4-12% Bis-Tris 10-well mini protein gels (Invitrogen, Germany) with 2-[N-morpholino]ethanesulfonic acid (MOPS ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were confirmed on a 0.8% agarose gel and visualized after staining with SYBR Safe DNA Gel Stain (Invitrogen, Carlsbad, CA). The amplicons were purified with an Invitrogen PCR Purification Kit (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: Protein profiles were analyzed by SDS-PAGE using pre-cast 4-20% acrylamide gradient gels (Novex WedgeWell, Tris-glycine gel; Thermo Fisher) and 20 μg protein per well ...
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bioRxiv - Microbiology 2021Quote: ... and separated using a 1.5% agarose gel stained with SYBR Safe DNA gel stain (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Bands of approximately 350 bp were excised by comparing to a 50 bp ladder (Bioline) ...
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bioRxiv - Microbiology 2020Quote: Protein samples were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using NUPAGE 10% Bis-Tris Gels (Life Technologies), run in NUPAGE MES Running Buffer (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The isolated protein samples were separated on 4-12% SDS-PAGE gels and were subsequently transferred to a nitrocellulose membrane using the iBlot Gel Transfer Device and iBlot Gel Transfer Stacks (Thermo Scientific). The blots were developed using LI-COR secondary antibodies (680RD and 800CW) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGE Bis-Tris gels (Thermo Fisher Scientic) and transferred on nitrocellulose membranes (iBlot 2® gel transfer Stacks Nitrocellulose system from Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... 15μl samples along with a 100bp DNA Ladder (GeneRulerTM, Fermentas) were run on a 2% agarose gel made with SYBR®Safe DNA gel stain (Invitrogen). Gels were then imaged using a Genius Bio Imaging System and GeneSnap Software (Syngene).
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bioRxiv - Cancer Biology 2022Quote: ... of SEC-purified small EVs and cell lysates were separated by SDS-polyacrylamide gel electrophoresis (PAGE; NuPAGE 4-12% Bis-Tris Protein Gel, ThermoFisher Scientific) and transferred to PVDF membranes (Millipore ...
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bioRxiv - Genomics 2022Quote: Native mitochondrial respiratory complexes were visualized by Blue Native PAGE (BN-PAGE) gels following the manufacturer’s instructions protocols (Nativepage 12% Bis Tris Protein Gels, 1.0 mm, 15 well, Thermo Fisher Scientific BN1003BOX). Mitochondrial pellets from 10 thoraxes or 10 larvae were resuspended in 20ul sample buffer cocktail (5µl sample buffer ...
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bioRxiv - Pathology 2022Quote: ... sites flanking the donor DNA sequence and the linearized donor DNA was purified by agarose gel electrophoresis with GeneJet Gel Extraction kit (Thermo Fisher). Linearized donor DNA and CRISPR/Cas9 complex were injected into C57BL/6 fertilized zygotes ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on a commercial native- PAGE gel (3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well, Invitrogen, Cat. BN1001BOX) at 100 V tension using as running buffer 50 mM BisTris ...
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bioRxiv - Cancer Biology 2019Quote: SDS-solubilized protein samples were resolved using the Novex NuPage SDS-PAGE gel system (ThermoFisher Scientific; 4-12% Bis-Tris gels), and transferred via semi-dry electrophoresis to 0.45 μm PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were resolved by 8% Native polyacrylamide gel electrophoresis (0.5X TBE buffer with 2mM MgCl2) run at 4°C and the gel was stained by SYBR Gold Nucleic acid Gel stain (ThermoFisher Scientific) for 30 min at room temperature in shaking condition ...
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bioRxiv - Molecular Biology 2020Quote: ... ref.17-1318-02) before being subjected to SDS-PAGE gel electrophoresis (NuPage™ 4-12% Bis-Tris Gel Invitrogen, NP0322box) and semi-dry transfer to a nitrocellulose membrane (iBlot®2NC Mini Stacks Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... after denaturation by 70°C for 3 minutes and separated by gel electrophoresis using 10% TBE-Urea gels (Invitrogen, Cat# EC68755BOX) at 180V for 60 min in 1x TBE electrophoresis buffer (Bio-Rad ...
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bioRxiv - Pathology 2021Quote: ... The PCR amplicon with an attB sequence at the end was visualized using gel electrophoresis and purified using the GeneJet Gel Extraction Kit (Thermo Scientific). Fragments were cloned into the pDONOR vector via a BP Clonase reaction (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Molecular Biology 2021Quote: ... and/or PCR products after the reaction were extracted from the gel and cleaned with a GeneJET Gel Extraction and DNA Cleanup Kit (ThermoFisher Scientific) according to the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were analyzed by 0.8% or 2% agarose gel electrophoresis and visualized under UV after staining with SYBR safe DNA gel stain (Invitrogen, Carlsbad, California). PCR products were purified with the Zymoclean Gel DNA Recovery Kit (Zymo Research ...