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Citations for Thermo Fisher :
1701 - 1750 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... RNAs were stained by incubating the gels in 50 ml 2X Invitrogen™ SYBR™ Gold nucleic acid gel stain (ThermoFisher) in 0.5x TB buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined via densitometry using BSA standards on an SDS- PAGE gel stained with Sypro Protein Gel Stain (ThermoFisher: S12000).
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bioRxiv - Biophysics 2023Quote: ... Proteins and lipids in the SEC fractions were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on pre-cast 4-12% gradient gel (Life technologies) and by LC-MS/MS ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... The same total protein amounts or extracts from the same number of cells were loaded into each lane of an SDS-PAGE gel (NuPAGE 4– 12% Bis-Tris Protein gels, Thermo Fisher) followed by transfer to a nitrocellulose membrane ...
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: The PCR products were separated by agarose gel electrophoresis and visualized using SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher Scientific). Images were captured with a WUV-M20 Printgraph Classic UV transilluminator (ATTO CORPORATION ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were treated with loading dye and electrophoresed in agarose gel stained with SYBER Safe DNA Gel Stain (Invitrogen: S33102) without cleanup and visualized under UV light ...
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bioRxiv - Neuroscience 2024Quote: ... They were subsequently separated through SDS-polyacrylamide gel electrophoresis on a NuPAGE Novex 4–12% Bis-Tris gel (Thermo Fisher Scientific) at 150V for 50 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... purified from the gel using the Nucleospin™ Gel and PCR Clean-up Kit and cloned into the pCRII-topo vector (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were analysed on a 1% agarose TAE gel stained with SYBR Safe DNA Gel Stain (Thermo Fisher Scientific S33102), and remaining products were purified using the QIAquick PCR Purification Kit (Qiagen 28104) ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were mixed with an equal volume of SYB Green loading buffer and operated electrophoresis on 2% agarose gel for quantification and qualification followed by purification with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was gel-purified and subjected to size-fractionation on a denaturing polyacrylamide gel (Novex TBE-urea 10%, Invitrogen, cat# EC68752BOX)) to enrich for small RNA molecules ...
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bioRxiv - Microbiology 2024Quote: ... and 450–600 bp amplicons were excised and extracted with the E-Gel SizeSelect 2% agarose gel system (Thermo Fisher Scientific). Amplicon libraries were sequenced by 2 × 250 bp paired-end sequencing on a MiSeq platform (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by gel electrophoresis using NuPAGE Novex 4–12% Bis-Tris precast gels and transferred onto PVDF membranes using the iBlot Gel Transfer Device (Thermo Fisher Scientific). Membranes were blocked with 5% non-fat milk in 0.2% Tween 20-phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Aliquots of all gradient fractions were analyzed on an SDS-polyacrylamide gel stained with Gel CodeTM Blue Safe Protein Stain (Thermo Fisher Scientific) and density measurement of the protein bands was done with ImageJ software.
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were detected by electrophoresis on 6% non-denaturing polyacrylamide/TBE gels with SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher Scientific). Splicing efficiency was measured by densitometry analysis of the PCR amplicons in image J (Schindelin et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-PCR products were run on either agarose gels with EtBr staining or PAGE gels with SYBR Gold staining (Thermo Fisher Scientific), and visualized on a Typhoon imager (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... 12 µg of protein per sample was used for gel electrophoresis with a NuPAGE™ 4-12% Mini Protein Gel (Invitrogen, NP0323) in a Mini Gel Tank (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and final product purity were assessed on a 15% sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using SYPRO Ruby Protein Gel Stain (S12000, Thermo Fisher Scientific). Refolding efficacy was measured by denaturing XCL1 with 20 µM beta-mercaptoethanol (-ME) ...
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bioRxiv - Molecular Biology 2021Quote: ... Products of both protocols were separated by DNA 10% polyacrylamide or 2% agarose gels and stained with SYBR Safe DNA Gel Stain (Thermo Fisher Scientific). ImageJ program was used to estimate indel or editing percentage measuring integrated intensity of digested and undigested products.
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bioRxiv - Microbiology 2019Quote: ... The PCR products were verified using standard TAE-agarose gel (1.5%) electrophoresis with the SYBR® Green I nucleic acid gel stain (Thermo Fisher Scientific), and the PCR products were purified using the GenepHlow Gel/PCR Kit (Geneaid).
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bioRxiv - Physiology 2019Quote: ... 15µl samples and a 100bp DNA ladder (GeneRuler™, Fermentas) were run on 2% agarose gels with SYBR®Safe DNA Gel Stain (Invitrogen). Gels were imaged using a Genius Bio Imaging System and GeneSnap software (Syngene) ...
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bioRxiv - Cancer Biology 2019Quote: ... The molecular sizes of peak 1 (300 kDa) and peak 2 (150 kDa) were determined by SDS gel electrophoresis (NuPAGE 4-12% Bis-Tris Gel; Life Technologies, CA) under non-reducing conditions along with antibody standards.
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bioRxiv - Genomics 2019Quote: ... 225-325 bp fragments (including the 125 bp NextFlex adapter) were size-selected using a 2% E-Gel Size Select agarose gel (Thermo Fisher Scientific) and amplified by PCR for ten (asexual blood stages and ookinetes ...
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bioRxiv - Physiology 2019Quote: ... Protein samples (100 µg/lane) were separated using a gradient gel (NuPAGE® Novex 4-12% Bis-Tris Gel 1.0 mm, Thermo Fisher Scientific) and transferred onto nitrocellulose membrane (Amersham Protran ...
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bioRxiv - Biochemistry 2019Quote: Western blotting was performed using mini gel tank system (Life Biotechnologies) with 4-12% gradient Bis-Tris gels (NuPage, Invitrogen cat. #NP0335) and 0.45 µm pore size nitrocellulose membranes (BioRad cat ...
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bioRxiv - Biochemistry 2020Quote: ... 20 µg of total protein/lane were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGETM 4-12% Bis-Tris SDS-PAGE Protein Gels (Thermo Fisher Scientific), and Western blot was completed using the Bolt Mini Blot Module (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... were loaded onto precasted Tris-Tricine 16% low molecular weight gels or pre-casted Tris-Glycine 4-20% gels (Thermo Fisher Scientific) and transferred onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... N-terminally acetylated and phosphorylated α-Syn strains were cleared from endotoxins using one run of Detoxi-Gel Endotoxin Removing Gel (Thermo Scientific) or until endotoxins were below detection level ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Lysate volumes with equivalent protein content were diluted with SDS-PAGE loading dye and subjected to gel electrophoresis on 4-12% BisTris SDS-PAGE gels (Life Technologies NP0322). Gels were then transferred and blocked in 5% NFDM for 1 hour at RT ...
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bioRxiv - Plant Biology 2020Quote: ... Amplified products were detected by gel electrophoresis of 10 μL of PCR reactions in a 1.5% TBE agarose gel with SYBR Safe dye (Thermo Scientific, Waltham, MA).
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were verified by electrophoresis in 2% agarose e-gel stained with SYBR™ Safe DNA Gel Stain (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Amplicons were first pooled in an equimolar fashion and then the pools were gel-extracted using the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific). The sequencing was carried out at the Interdisciplinary Center for Biotechnology Research (ICBR ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products and products of restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). DNA sequences were analyzed by DNA sequence service of the University of Alberta Molecular Biology Service Unit and Fasmac Co. ...
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bioRxiv - Neuroscience 2020Quote: ... PCR product was checked by agarose gel-electrophoresis using 2% gel (SeaKem LE agarose) and GeneRulerTM 100 bp Plus DNA ladder (#SM0328, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Assessment of the quality of the isolated DNA was performed by electrophoresis on 0.8% agarose gels stained with SYBR™ Green I Nucleic Acid Gel stain (Invitrogen, Carlsbad, CA) and visualized using a UV transilluminator (Ultra-Lum Inc ...
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bioRxiv - Microbiology 2019Quote: ... and heated for 2 minutes at 85°C or 95°C before gel electrophoresis on a precast 10-20% Tricine gel (Thermo Fisher Scientific) in Tricine running buffer (100 mM Tris base [pH 8.3] ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were loaded onto a gel and separated by SDS-PAGE using NuPAGE® pre-cast gels (10 % or 4-12 %) (Thermo Scientific). Protein was transferred onto PVDF membrane and blocked and incubated with primary antibody in 5 % non-fat milk or BSA diluted in PBS-Tween 20 ...
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bioRxiv - Microbiology 2022Quote: ... Heterologous expression of HpuA was confirmed by protein gel analysis (Novex 10-20% Tris-Glycine mini wedge well gels, Thermo Fisher Scientific) followed by Coomassie blue staining (Bio-Safe Coomassie G-250 ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples were resolved by SDS–polyacrylamide gel electrophoresis in NuPAGE Novex 4 -12% Bis-Tris gels (ThermoFisher Scientific, NP0321 and WG1402A) either with NuPAGE MOPS SDS buffer (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The subcellular fractionation samples were resolved by denaturing gel electrophoresis on 4-12% Bis-Tris gradient protein gels (Thermo Fisher Scientific, NP0335BOX) using MES SDS running buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA fragments between 200 bp and 1 Kb were size-selected and recovered after resolving on an E-Gel EX Precast Agarose Gels (Thermo Fisher Scientific). Library molarity concentration calculation was obtained by measuring concentration of double stranded DNA (Qubit ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblotting of MSD2-mCherry was performed after native-PAGE or SDS-PAGE (12.5% separating gel and 4% stacking gel) with anti-mCherry primary antibody (Invitrogen, cat. n. M11217) diluted to 1:5,000 in Tris-buffered saline with 0.1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... The fragments originating from mono-nucleosomes + 125 bp NextFlex adapter were selected using 2% E-Gel Size Select agarose gels (Invitrogen, #G6610-02) and amplified by PCR for nine cycles using the above conditions ...
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bioRxiv - Neuroscience 2021Quote: ... Samples (12-15 μg per sample per lane) were loaded and run on a 10%-20% tricine gel (Novex 10%-20% Tricine Protein Gel, Thermo Fisher Scientific), and transferred onto a polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2020Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride (PVDF) membrane using an iBlot™2 Gel Transfer Device (ThermoFisher Scientific) at 20 V for 6 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cell-free synthesized protein was visualized by Coomassie blue staining after protein gel electrophoresis using pre-casted 4-12% Bis-Tris gradient gel (Invitrogen, Waltham, MA) (Figure S5).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Equal protein concentrations were run on a 10% SDS-PAGE (polyacrylamide gel electrophoresis) gel and transferred to a polyvinylidene difluoride membrane (Thermo Fisher Scientific). Membranes were exposed to anti-p65 ...