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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Ethyl 5 4 methoxyphenyl 5 oxovalerate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 4 and 5 we confined cells by using a soft acrylamide gel and either 1% Low Melting Agarose (Invitrogen, 16520-100) or a second soft acrylamide gel ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the samples were run on a thermocycler (60 min, 37°C; 5 min, 95°C; held, 4°C; Applied Biosystems); cDNA was stored at −20°C until use.
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Physiology 2024Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Microbiology 2023Quote: ... Blocking was performed for 1h (5% skimmed milk in Tris-buffered saline containing 0.5% Tween) and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A ...
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bioRxiv - Microbiology 2023Quote: ... the media from each well was aspirated and the wells were overlaid with 4 ml/well DMEM with 5% FBS and 0.7% agarose (Invitrogen, 16500-500). At 7 dpi ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated for 5 min at 99°C before loading on 4-12 % SDS-PAGE with MOPS as running buffer (Thermo-Fisher Life Technologies). BenchMark protein ladder was used as marker (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed again at 1224 rpm for 5 minutes at 4 degrees Celsius and then counted using an automated cell counter (Countess, Thermo Fisher) to obtain the cell concentrations ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Microbiology 2022Quote: Cultures taken from exponential growth at OD 0.5 are starved according to the above protocol and stained with 5 μg/ml DiBac3(4) and 1.25 μg/ml Propidium Iodide (Thermo Fisher Scientific) 10 minutes after entry to starvation ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was then washed 4 times for 5 minutes each with deionized water and stained with Imperial Blue stain (ThermoFisher #24615) for two hours before washing in deionized water overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Microbiology 2022Quote: ... Q-PCR was performed using PowerUp SYBR Green Master Mix with 4 replicates and an Applied Biosystems QuantStudio™ 5 real-time PCR instrument (ThermoFisher). E ...
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bioRxiv - Molecular Biology 2023Quote: ... 50,000 cells swollen after hypotonic shock in 200 μl of KCl 75 mM were cytospun on a slide for 5 min at 900 rpm (Cytospin 4, Thermo Fisher). These cells were then fixed in 3% paraformaldehyde/PBS for further immunolabelling.
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... incubated in secondary antibody solution for 4 hours at RT (5% normal goat serum in PBST, Alexa Fluor 633 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Cancer Biology 2023Quote: ... a final extension step (68°C for 5 min) and holding the reaction at 4°C in a thermocycler (Applied Biosystems) with a preheated lid (105°C) ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cell suspension was passed through a 70-μm filter, centrifuged (400xg, 5 minutes, 4 °C) and resuspended in Advanced DMEM/F12 (ThermoFisher #12634010) supplemented with FCS (10% vol/vol ...
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bioRxiv - Immunology 2024Quote: ... For T cell panels (Supplemental table 2, 4, 5, 6) we fixed cells with the FOXP3 Fixation/Permeabilization Buffer Kit (Thermo Fisher) and conducted intracellular/nuclear stains using the FOXP3 Permeabilization Buffer (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... was prepared fresh with the 3’aminated 5’ caged oligo root carrying a terminal Cy3 dye (2 μM BL003 for the single-cycle experiment and 2 μM BL728 for the 4-cycle experiment) and a bifunctional crosslinker BS(5)PEG (Thermo Scientific) (5 μM in dimethylformamide) ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed with 5 mL PBS (4°C) and trypsinized with 1 mL 0.25% Trypsin-EDTA per dish (Gibco, 25200-056) for 5 min at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were subsequently permeabilized with 0.5% Triton solution for 5 min at 4°C and then incubated with PicoGreen (1:1000 dilution) (Cat No: P11496, ThermoFisher Scientific) in PBST buffer (PBS plus 0.1% Tween-20 ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were washed again at 1224 rpm for 5 minutes at 4 degrees Celsius and then counted using an automated cell counter (Countess, Thermo Fisher) to obtain the cell concentrations ...
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bioRxiv - Physiology 2024Quote: Intracellular Ca2+ [Ca2+]i measurements were performed in cardiomyocytes loaded with Fluo-4 AM dye (5 μmol.L-1, ThermoFisher, Cat #F14201). After a 30-minute loading period ...
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bioRxiv - Neuroscience 2024Quote: LDS-treated samples were thawed and boiled at 95°C for 5 minutes before loading into 1.0 mm-thick Bis-Tris 4-12% polyacrylamide gels (Invitrogen, cat# NP0323) and subjected to electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... The fixed cells were spun down at 900 × g for 5 min at 4°C and then washed twice with 1 ml of ice-cold PBS (Gibco 70011044). Cells were lysed twice with 1 ml of ice-cold 4C permeabilization buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 μl of the cytoplasmic fraction and 5 μl of the mitochondrial fraction were loaded onto a 4-12% Bis-Tris gel (Invitrogen, NP0322BOX) and run using MES buffer at 100V for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed with 23G needles and the parasites passed through a 5 µm filter before fixation in 4% PFA and staining with Hoechst 33342 (Thermofisher Scientific). Samples were analysed on a BD LSR Fortessa flow cytometer and with FlowJo software v10 ...
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bioRxiv - Biophysics 2024Quote: Aliquots of 3 μL of complex sample was applied on UltrAuFoil r1.2/1.3 Grids covered with Graphene Oxide as described before76 and the grids were blotted for 3.5 s at force -1 at 4°C and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 26,386 Movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... 35 μg of protein lysate was heated at 95 °C for 5 min and loaded onto Novex Tris-Glycine Mini-Protein Gel 4–12% (ThermoFisher #XP04200BOX) and run at 100V for 1.5 h ...
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bioRxiv - Immunology 2024Quote: ... murine lung or spleen cells were stimulated for 4 hrs with Ancestral spike (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies) for a further 10-12 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... Protein isolates were mixed with protein loading buffer containing 100 mM DTT reducing agent and denatured for 5 min at 95°C before loading onto 4–12% Bis-Tris NuPAGE precast gels (Thermo Fisher) for electrophoresis ...
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bioRxiv - Systems Biology 2024Quote: ... about 10 cm of leaf length were cut into small pieces and placed in chilled 5-mL tubes containing 4 mL of RNALater (Fisher Scientific) solution for RNA stabilization and storage ...
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bioRxiv - Neuroscience 2024Quote: ... The RNase I treatment was carried out as described by Ingolia et al.19 for 45 min at 4 °C using 5 µl of RNase I (Ambion, AM2295) per 250 µg of crude total RNA and quenched by adding 20 µl of SUPERase-In (Ambion ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the samples were run on a thermocycler (60 min, 37 °C; 5 min, 95 °C; held, 4 °C; Applied Biosystems); cDNA was stored at −20 °C until use.
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bioRxiv - Neuroscience 2024Quote: ... The protein mixture was boiled at 95 °C for 5 minutes before run on a 4-12 % Bis-Tris gel (Invitrogen, #NP0335BOX) in NuPAGE MOPS buffer (ThermoFisher ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... 5 µL of the lysates were resolved on a NuPAGE 4-12% Bis-Tris Protein Gel with MOPS buffer (Thermo Scientific) and transferred to a 0.45 µm nitrocellulose membrane (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... Protein– antibody complexes were visualized using the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium for color development (Life Technologies).
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bioRxiv - Genetics 2024Quote: ... Samples were heated at 95°C for 5 min (protein denaturation step) before loading onto a 4–12% NuPAGE precast gel for Western analysis (ThermoFisher Scientific). After the transfer to PVDF membranes (LC2005 ...
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bioRxiv - Biophysics 2024Quote: ... 1 µL of the completed PCR reactions was then combined with 4 µL of water and 5 µL of print buffer (2x: 20 mg/mL UltraPure BSA (Thermo Fisher), 24 mg/mL trehalose dihydrate ...
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bioRxiv - Cancer Biology 2024Quote: ... Radiation-induced alterations in ZFE length were measured at 5 days post-fertilization (dpf) following embryo fixation (4% PFA) and microscopic imaging (Evos XL Core Cell Imaging System, Thermo Fisher). Analysis was conducted using ImageJ software ...