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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Ethyl 5 4 methoxyphenyl 5 oxovalerate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Molecular Biology 2022Quote: ... then the supernatant was diluted 10x and pre-cleared for 30 min at 4 °C with rotation using 5 µl protein A- and G-coupled dynabeads (ThermoFisher Scientific). An input sample was taken ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Genetics 2022Quote: ... Ly6Chi monocytes were labeled by intraperitoneal injection of 4 mg/mL of Edu (5-ethynyl-2’-deoxyuridine) from Life technologies (NY), and mice were euthanized after 5 days to assess baseline recruitment or 21 days to assess for retention ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 21,000g for 20 min at 4 °C and 5 μl/ml of RNase A/T1 (Thermo Fisher Scientific) was added to the supernatants ...
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bioRxiv - Pathology 2020Quote: ... and resolved on 4-12% Bis-Tris Plus Bolt™ gels at 4-5 ug of protein per lane in MES SDS Running Buffer (Invitrogen). Target-protein signal was detected with Horseradish Peroxidase (HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heated to 95 °C for 5 min and cooled on ice prior to loading on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher). SDS-PAGE was performed of all compared samples in parallel according to the manufacturer’s specification ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Cancer Biology 2020Quote: ... incubated in ice for 5 min and then loaded into to a pre-cast gel (NuPAGE® 4-12% Bis-Tris Protein Gels, Invitrogen). Gel electrophoresis was performed for 90 min at 125V ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Immunology 2020Quote: ... and heated at 70°C for 5 min before separation on 4-12% Bis-Tris Plus ready gel (Thermo Fisher Scientific). To determine PGE2R glycosylation in the hemolymph samples ...
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bioRxiv - Genetics 2020Quote: ... boiled in reducing sample buffer for 5 min and resolved on 4–12% Bis-Tris Bolt gels and transferred using an iBlot 2 (Thermo Fisher). Blots were blocked in 2.5% milk in 1% TBS-Tween before staining with antibodies (Table S5) ...
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bioRxiv - Neuroscience 2021Quote: ... prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556, Thermo Fisher Scientific, 1:500) or Alexa Fluor680 (A31556 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBS-0.5%Triton X-100 at RT before overnight incubation at 4°C with Alexa555-conjugated donkey-anti-rabbit antibody (Invitrogen, #A32794) in PBS containing 5% NHS ...
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bioRxiv - Developmental Biology 2021Quote: ... Collected cells were then pelleted at 0.8 × 1000g for 5 minutes at 4 degrees Celsius and resuspended in Trizol (Thermo Fisher 15596018). After genotyping ...
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bioRxiv - Biochemistry 2021Quote: ... and insoluble samples and were denatured in reducing Laemmli buffer at 100°C for 5-10 min prior to loading onto a 4-20%Tris-Gly SDS-PAGE gel (Life Technologies). Proteins were then transferred to a nitrocellulose membrane using an iBlot2 (Life Technologies) ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was exchanged daily and splitting was performed every 4-5 days when cells reached 75-85% confluency using TrypLETM (Gibco, 12605028). Cultures were propagated for at most 4 passages before being replaced by fresh cell stock.
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bioRxiv - Cell Biology 2020Quote: ... A pool of 4 siRNAs targeting mouse SNAP-47 and control Luciferase siRNA (Target Sequence: 5’-CGTACGCGGAATACTTCGA-3’) (Dharmacon, Thermofisher Scientific) were electroporated along with GFP into E15.5 cortical neurons (2 µg GFP + 50 pmol siRNAs) ...
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bioRxiv - Immunology 2021Quote: ... The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyl phosphate p-toludine salt (NBT/BCIP chromagen) substrate solution (Thermo Scientific) for 7 min ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated capillary networks were placed onto a glass coverslip and fixed (4% formaldehyde in phosphate buffered saline (PBS)) and blocked (0.5% Triton X-100, 5% SEABlock Blocking Buffer (ThermoFisher Scientific, USA) in PBS ...
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bioRxiv - Microbiology 2022Quote: Cultures taken from exponential growth at OD 0.5 are starved according to the above protocol and stained with 5 μg/ml DiBac3(4) and 1.25 μg/ml Propidium Iodide (Thermo Fisher Scientific) 10 minutes after entry to starvation ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was then washed 4 times for 5 minutes each with deionized water and stained with Imperial Blue stain (ThermoFisher #24615) for two hours before washing in deionized water overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Microbiology 2022Quote: ... Q-PCR was performed using PowerUp SYBR Green Master Mix with 4 replicates and an Applied Biosystems QuantStudio™ 5 real-time PCR instrument (ThermoFisher). E ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical neurons were pelleted at 2,500 x g for 5 minutes at 4 °C and resuspended in 1 mL Neurobasal outgrowth media (Thermo Scientific, 21103049), supplemented with B-27 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with excess DTT was added to all reactions and samples were fixed for 5 min at 95°C before loading on a 4-12% Bis-Tris gel (Invitrogen WG1402BOX). The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211 ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... with 5% β-mercaptoethanol at 50°C for 10mins and loaded onto a 4-20% Tris-glycine SDS-PAGE gel (Invitrogen). The proteins were transferred to a nitrocellulose membrane with an iBlot (ThermoFischer) ...
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bioRxiv - Biochemistry 2022Quote: Phagosomes or cells were lysed directly in 2x Laemmli buffer (with 5% ß-mercaptoethanol) and subjected to SDS-Page using 4-12% NuPAGE gels (Invitrogen) and immunoblotted to PVDF membrane ...
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bioRxiv - Neuroscience 2022Quote: ... 800 µL of the bottom phase was transferred to scintillation vials containing 5 mL ScintiVerse BD Cocktail (Fisher Scientific SX18-4). The vials were vortexed and radioactivity was measured in a Scintillation Counter (Tri-Carb 2819TR ...
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bioRxiv - Microbiology 2022Quote: ... A 3% agarose gel with 4 μL ethidium bromide was loaded with 5 μL PCR reaction and 2 μL GeneRuler 100bp ladder Plus (Thermo Fisher) and run for 40 minutes at 100 volts ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were transferred to the dialysis wells plates and incubated at 37°C and 5% CO2 for a total 4 weeks in osteogenic monoculture medium containing DMEM (low glucose, 22320, Thermo Scientific), 10% human platelet lysate [11] (hPL ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... the neurons were given 12 hours in growth media at 37°C/5% CO2 to allow sufficient recovery and expression of the plasmid prior to fixation in 4% formaldehyde (Thermo Fisher) / 4% sucrose (VWR ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Physiology 2024Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Neuroscience 2024Quote: ... zebrafish larvae from visually similar developmental stages were collected in groups of 16 individuals from all corresponding genotypes (N = 4-5 per genotype) and stored in RNAlater (Thermo Fisher) until use ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Genomics 2024Quote: ... The purified DNA solutions from the eight aliquots were combined and loaded into 5 lanes of 4% E-gel (Invitrogen, G401004). The DNA bands with the size between 300 bp and 1.2 Kb were excised ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then incubated overnight at 4 °C with 5 μg of prebound anti-SP100 or rabbit IgG isotype control protein A dynabeads (Thermo Fisher). The next morning ...
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bioRxiv - Immunology 2023Quote: ... at 1:100 overnight at 4°C in 5% BSA in the presence of 0.05% sodium azide (BP922I-500; Thermo Fisher Scientific), 0.5% Triton X-100 (161-0407 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were centrifuged at 400 g for 5 min at 4 °C with no brake and resuspended in staining buffer (DPBS (without Ca2+ and Mg2+) (Gibco, Life Technologies) containing 0.1 % low endotoxin BSA (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: Protein extracts were boiled with NuPAGE 4x sample buffer containing 50 mM DTT for 5 min and then loaded onto NuPAGE Novex 4-12% Bis-Tris gels (ThermoFisher Scientific). Electrophoresis was performed according to a standard protocol followed by transfer of proteins onto PVDF membrane (pore size 0.2 µm ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of eluted ChIP-DNA were mixed with 5 µL of PowerUp SYBR Green master mix (Applied Biosystems, cat#A25743), and 1 µL of the primer mix (forward+reverse ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4-20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Differentiation medium was replaced daily on days 4 and 5 by 75:25 KSR:N2 medium (486.5 mL DMEM/F-12 (Thermo Fisher Scientific, 11320033), 5 mL 15 % glucose ...