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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... insertions were additionally verified by flanking fragment sequencing (Supplementary Fig. 7) using the Phire Plant Direct PCR Kit (Thermo Fisher Scientific) and primers listed in Supplementary Table 1 ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was isolated from the progeny at the L2-L3 larval stage according to the protocol of the Phire Tissue Direct PCR Kit (Thermo Scientific). Multiplex PCR was performed with primers 260-q-Mag-Mel-R ...
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bioRxiv - Systems Biology 2022Quote: A final volume of 10 µL of purified mRNA was obtained using the Dynabeads® mRNA Direct Kit (ThermoFisher, Cat#61012) from an optimized protocol to work in 96-well plates 42.
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bioRxiv - Cancer Biology 2019Quote: ... Clones harboring a nonsense mutation were determined by PCR-direct sequencing using BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific).
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bioRxiv - Physiology 2021Quote: ... Poly(A) RNA was isolated using the Dynabeads mRNA DIRECT Kit according to the manufacturer’s instructions (Life Technologies, Carlsbad, CA, USA) and then subjected to reverse transcription (RT ...
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bioRxiv - Microbiology 2021Quote: ... 3 μg of total RNA from the same yellow-necked field mice were treated with the Dynabeads mRNA DIRECT Micro Purification Kit (Invitrogen, USA) following the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... and mutant detection was performed on adult legs with the Phire Animal Tissue Direct PCR kit (Thermo Fisher Scientific, Waltham, MA) with added LCGreen Plus+ Melting dye (Idaho Technology Inc. ...
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bioRxiv - Genomics 2021Quote: ... the mRNA captured on beads was washed extensively according to the manual of Dynabeads® mRNA DIRECT™ Micro Kit (Invitrogen). The Dynabeads-mRNA complex was then resuspended in 20 μl of reverse transcription reaction mix without any primer prepared using SuperScript™ IV First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: The immunoprecipitated part of the library was then used for amplification of the insert region according to the manufacturer’s instructions with a Phusion Blood Direct PCR Kit (Thermo Fisher Scientific). The primers T7_Endo_Lib_LONG_FOR GCCCTCTGTGTGAATTCT and T7_Endo_Lib_LONG_REV GTCACCGACACAAGCTTA were used and a second round of PCR was carried out with the IDT for Illumina UD indexes (Illumina Corp. ...
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bioRxiv - Plant Biology 2022Quote: ... Individual T0 plants carrying homozygous or biallelic mutations generated by CRISPR were identified by Sanger sequencing (AGRF) of the targets and flanking region amplified by the Phire Plant Direct PCR Kit (ThermoFisher Scientific).
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bioRxiv - Immunology 2023Quote: ... was then used for amplification of the insert region according to the manufacturer’s instructions with a Phusion Blood Direct PCR Kit (Thermo Fisher Scientific). A second round of PCR was carried out with the IDT for Illumina UD indexes (Illumina Corp. ...
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bioRxiv - Cancer Biology 2023Quote: RNA from sorted NK cells and fibroblasts was purified using the Dynabeads mRNA DIRECT Purification Kit (Thermo Fisher Scientific; cat. #61012). A total of 1 × 104 pNKs or fibroblasts were isolated and sorted into 40 μL of lysis/binding buffer ...
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bioRxiv - Immunology 2024Quote: ... and gDNA was isolated from the offspring L2-L3 larvae according to the storage & dilution protocol of the Phire Tissue Direct PCR Kit (Thermo Scientific). Multiplex PCR was performed with primers 530-P2A-multi-F ...
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bioRxiv - Plant Biology 2023Quote: ... ELISAs were conducted with a fully automated robotic system (Automated ELISA Workstation, Thermo Fisher Scientific Inc. Waltham, MA, USA). The ELISA response values are depicted as heat maps to reflect the relative abundance of glycan epitopes recognized by the mAbs.
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bioRxiv - Neuroscience 2019Quote: Total tau and pT231 tau content were measured by commercial tau ELISA kits according to the manufacturer’s instructions (total tau – KHB0041; pT231 tau – KHB8051, Invitrogen). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Neuroscience 2019Quote: ... These brain extracts were analyzed by sandwich ELISA for Aβ1-40 and Aβ1-42 using commercial ELISA kits and following the manufacturer’s protocol (Aβ1-40: KHB348, and Aβ1-42: KHB3441, Thermo Fisher Scientific). The Aβ concentration was calculated by comparing the sample absorbance with the absorbance of known concentrations of synthetic Aβ1-40 and Aβ1-42 standards on the same plate ...
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bioRxiv - Neuroscience 2022Quote: The total amount of soluble tau in S1 fractions was determined by using an ELISA kit (human Tau, Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The supernatants were collected and quantified for soluble Aβ40 and Aβ42 using enzyme-linked immunosorbent assay (ELISA) kits (Invitrogen, US) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: The quantitation of soluble human Aβ1-42 in hippocampus extracts was determined with a sandwich antibody ELISA kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... APOE levels were similarly processed and quantified from iMGL conditioned media using Apolipoprotein E Human ELISA kit (Invitrogen; #EHAPOE).
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bioRxiv - Immunology 2019Quote: ... and IL-18 were determined using the Platinum ELISA kit for the quantitative detection of the three cytokines (ThermoFisher SCIENTIFIC Invitrogen ...
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bioRxiv - Immunology 2020Quote: The IFN-γ concentration in the sera was analyzed by standard sandwich ELISA kit according to manufacturer’s instructions (ThermoFisher).
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bioRxiv - Immunology 2020Quote: TNF-α released by mouse peritoneal macrophages was measured by a Mouse TNF-α ELISA Kit (Invitrogen, BMS607-3TEN) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: The concentration of IFN-γ was determined in the cultural medium with an ELISA kit according to the manufacturer’s protocol (Cat. 88-7314-22, ThermoFisher). In CD8+ T cells ...
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bioRxiv - Immunology 2022Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22 ...
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bioRxiv - Cell Biology 2022Quote: ... Human AFP secreted into the sera of animals were determined by the Human AFP Elisa Quantification Kit (Invitrogen, EHAFP) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Supernatants (25 μl) were used to measure MPO using the Myeloperoxidase Mouse ELISA Kit (Thermo Fisher Scientific, Catalog # EMMPO). Plates were read at 450 nm using a Varioskan Instrument.
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bioRxiv - Neuroscience 2023Quote: ... These brain extracts were analyzed by sandwich ELISA for Aβ1-40 and Aβ1-42 using commercial ELISA kits and following the manufacturer’s protocol (Aβ1-40: KHB348 and Aβ1-42: KHB3441, Thermo Fisher Scientific). The Aβ concentration was calculated by comparing the sample absorbance with the absorbance of known concentrations of synthetic Aβ1–40 and Aβ1–42 standards on the same plate ...
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bioRxiv - Neuroscience 2022Quote: ... conditioned neuronal media was diluted and handled according to the protocol of the Human Aβ42 Ultrasensitive ELISA Kit (Invitrogen) followed by colorimetric readout at 450 nm.
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ concentrations in three separate fractions of brain homogenates were determined by Invitrogen™ Amyloid β 42 and 40 Human ELISA kits (Cat.-No.: KHB3441 and KHB3481, respectively; both from Thermo Fisher Scientific). Results were normalized on the basis of the sample’s protein concentration.
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bioRxiv - Immunology 2023Quote: ... Fecal samples were then serially diluted and analyzed for IgA concentration by ELISA using a commercially available kit following manufacturer’s protocol (Invitrogen). Reading was acquired in a microplate reader (MultiSkan FC microplate photometer).
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bioRxiv - Biochemistry 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a human IL-8 ELISA kit (Invitrogen).
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bioRxiv - Synthetic Biology 2023Quote: ... We quantified the concentration of IL-22 using an Invitrogen Mouse IL-22 ELISA Kit (Invitrogen, catalog no. BMS2047).
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bioRxiv - Neuroscience 2023Quote: ... The levels of Aβ40 and Aβ42 were measured using Aβ40- and Aβ42-specific ELISA kits (Invitrogen KHB3481 and KHB3441) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Immunology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... The levels of soluble and insoluble human Aβ40 and Aβ42 in these extracts were quantified using ELISA kits (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Enzyme-linked immunosorbent assay (ELISA) kits were used according to the manufacturer’s instructions (R&D systems, Raybiotech or Thermofisher) and analyzed with a BioTek Synergy H1 plate analyzer ...
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bioRxiv - Immunology 2021Quote: ... then TMB substrate (1-Step™ Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, Waltham, MA, USA) was added and developed for approximately 1 minute before stopping the reaction with 1 N HCl ...
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bioRxiv - Neuroscience 2021Quote: ... ISF Aβ42 in the 14 samples for each mouse was measured by ELISA (Invitrogen, KHB3441, 1:4). To determine Aβ42 half-life (Cirrito et al. ...
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bioRxiv - Immunology 2021Quote: ... the assay was developed by addition of 1-Step Ultra TMB-ELISA solution (Thermo Scientific Cat# 34028) and reaction was stopped with 2 N sulfuric acid (Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... A colorimetric detection was performed using a 1-Step Ultra TMB ELISA substrate solution (Thermo Fisher Scientific) and OD450 was measured with a SpectraMax Plus 384 microplate reader (Molecular Devices) ...
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bioRxiv - Bioengineering 2021Quote: ... A colorimetric detection was performed using a 1-Step Ultra TMB ELISA substrate solution (Thermo Fisher Scientific) and OD450 was measured with a SpectraMax Plus 384 microplate reader (Molecular Devices).
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bioRxiv - Microbiology 2020Quote: ... was performed prior to incubation with 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific, CA) for color development ...
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bioRxiv - Immunology 2022Quote: ... Horseradish peroxidase activity was detected with 50 µL 1-Step Ultra TMB ELISA substrate solution (Thermo Fisher). Then the reaction was stopped with 50 µL 10% H2SO4 and the absorbance was measured at 450 nm ...