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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... b-galactosidase activity was measured using the Tropix Galacto-Light Plus™ chemiluminescent kit according to the manufacturer’s protocol (Applied Biosystems, Foster City, CA).
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bioRxiv - Microbiology 2022Quote: ... β-galactosidase activity was measured using the Tropix Galacto-Light Plus™ chemiluminescent kit according to the manufacturer’s protocol (Applied Biosystems, Foster City, CA).
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bioRxiv - Cancer Biology 2023Quote: ... were probed against primaries 45 minutes at room temperature and detected bands were visualised using ECL reagent (NovexTM ECL Chemiluminescent Substrate Reagent Kit, Thermo Fisher Scientific, Massachusetts, USA) and processed using Image LabTM software 6.0.1 (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... and transferred onto a nitrocellulose membrane (Cytiva, cat#10600001) for Western blotting or for RNA detection using a Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific, cat#89880).
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bioRxiv - Neuroscience 2021Quote: ... tau proteins were measured with Tau-5 antibodies using ELISA according to manufacturer’s instructions (The Human Tau Solid-phase sandwich ELISA, #KHB0041, Thermo Scientific).
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: ... Cytokine levels of IL-17a and IFNγ were quantified from collected supernatant using Mouse IL-17a Uncoated ELISA and Mouse IFN gamma Uncoated ELISA (Invitrogen) Kits ...
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bioRxiv - Cell Biology 2021Quote: ... was applied for 1 h at room temperature and the signal visualized using SuperSignal enhanced chemiluminescent (ECL) reagent (Thermo Fisher Scientific, #34080).
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bioRxiv - Cell Biology 2020Quote: ... Membranes were further incubated with chemiluminescence substrate (SuperSignal West Femto Maximum Sensitivity Substrate, or SuperSignal™ West Pico PLUS Chemiluminescent Substrate) for 1 minute (Thermo scientific). Protein bands were visualized with the Fusion FX UILBER LOURMAT (Vilber) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer) for 1 hour at room temperature and detected using SuperSignal Western Dura PLUS Chemiluminescent Substrate (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (Pierce, 1:5000) and chemiluminescent HRP substrates (Supersignal West Pico PLUS, Thermo Fisher Scientific; Pierce ECL Plus, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... after incubation with a secondary anti-rabbit antibody coupled with the horseradish peroxidase (HRP, 1:5000) and exposure to the SuperSignal West Femto chemiluminescent substrate (Thermo Fisher Scientific). Densitometric quantification of the bands was performed with ImageLab software (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An RNA sequencing library was generated from 500 ng of total RNA using a Dynabeads® mRNA DIRECT(tm) Micro Purification Kit (Thermo Fisher) for mRNA purification followed by a NEBNext® Ultra(tm ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Physiology 2021Quote: ... Poly-A mRNA was directly isolated from frozen EDL samples by using the Dynabeads™ mRNA DIRECT™ Purification Kit (61011, Invitrogen) with the Mini configuration followed by alkaline hydrolysis ...
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bioRxiv - Developmental Biology 2022Quote: ... eggs were individually homogenised in 5ul DEPC water within the PCR tube and 1ul was used for gDNA extraction using Phire Animal Tissue Direct PCR Kit (ThermoFisher, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The cells were washed once with PBS and fixed and permeabilized for 30 minutes avoiding direct light at RT using the fixation and permeabilization kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitations were done by using covalently labelled IgG fraction of rabbit antibodies to mouse CAR (rb80) to agarose beads by sodium cyanoborohydride and by following the instructions of the Pierce Direct IP Kit (Thermo Scientific, #26148). A HeLa cell line stably expressing connexin43 [47] grown in DMEM/10%FCS/P/S supplemented with 1 μg/ml puromycin (Sigma #P9620 ...
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bioRxiv - Genomics 2022Quote: ... Two rounds of poly(A)+ RNA purification were performed for all RNA samples using the Dynabeads® mRNA DIRECT™ Micro Kit (Invitrogen™/Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 130 μm nozzle was used and 1,000 to 5,000 cells were sorted into 1.5 ml Eppendorf tubes containing 10 μl of dilution buffer from the Phire Tissue Direct PCR Master Mix kit (F160L, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Variability due to loss of material during centrifugation steps was minimized by directly performing qPCR on aliquots of PMA-treated and non treated parasites after adding the DNARelease Additive from the Phire tissue direct PCR Master kit (Thermo Fisher Scientific) without any gDNA purification.
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bioRxiv - Neuroscience 2023Quote: Sequences were then determined directly by the Sanger method on an ABI 3730 genetic analyser using the BigDye direct cycle sequencing kit (ThermoFisher Scientific, USA). Presence or absence ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was eluted in 100 µl of water and subjected to mRNA polyA enrichment using the Dynabeads mRNA DIRECT Micro kit (Thermo Fisher 61021). After two rounds of washing and enrichment of mRNA following the manufacture’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... followed by DNA isolation and PCR amplification of CRISPR-targeted loci using Phire Tissue Direct PCR Master Mix kit (ThermoFisher, Waltham, MA). Base editing efficiency was analyzed at the genomic level by Sanger sequencing of the PCR amplicons ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA from the lysate supplemented with the purified mRNA-ribosome complexes was extracted using TRIzol LS reagent and a Direct-zol RNA MicroPrep Kit and treated with a TURBO DNase (Thermo Fisher Scientific). cDNAs were synthesized using the ReverTraAce qPCR RT Kit (TOYOBO) ...
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bioRxiv - Pathology 2021Quote: ... Cells grown for 24h in 6-well plates were transfected with 1 μg of cDNA construct (Asic2b or t-Asic2b) using a Lipofectamine™ Plus kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... the media was decanted and cells were incubated for 1 h at 37 °C with Fluo-4 Direct dye (Invitrogen) in FLIPR buffer (1× HBSS ...
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bioRxiv - Immunology 2020Quote: ... and cytokine concentrations measured by ELISA (Thermo Fisher).
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bioRxiv - Evolutionary Biology 2022Quote: ... 3,3′,5,5′-Tetramethylbenzidine ELISA substrate solution (ThermoFisher Scientific) was added and incubated for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and cytokine levels were measured by ELISA (Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Systems Biology 2022Quote: ... ELISA assays for IL-6 (Invitrogen, catalog # KHC0061) and IL-8 (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Mouse TNF alpha Uncoated ELISA (Invitrogen, #88-7324); IL-1 alpha Mouse Uncoated ELISA Kit (ThermoFisher #88-501988) ...
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bioRxiv - Microbiology 2021Quote: Soluble MUC1 was quantified by ELISA (EHMUC1, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... SuperSignal™ ELISA Femto Maximum Sensitivity Substrate (ThermoFisher) was added to plates following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... SuperSignal™ ELISA Femto Maximum Sensitivity Substrate (ThermoFisher) was added to plates following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... One-step TMB-ELISA (34028, ThermoFisher, MA, US) was used to detect absorbance on a plate reader at 650 nm.
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bioRxiv - Cell Biology 2023Quote: ... TMB (3,3′,5,5′-tetramethylbenzidine) ELISA Substrate (ThermoFisher Scientific) was added for 15 mins ...
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bioRxiv - Immunology 2023Quote: ... ELISAs were developed using ABTS solution (Thermo Fisher) and absorbance was measured at 415 nm and 695 nm by a plate reader (Tecan) ...
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bioRxiv - Immunology 2024Quote: ... Samples were blocked with ELISA blocking diluent (Invitrogen) for 2 hr at 37°C then washed 3X ...
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bioRxiv - Bioengineering 2024Quote: ... and assayed with an IL-10 ELISA (Invitrogen). IL-10 and Fab-IL-10 generated in our laboratories served as the standards for their respective ELISAs ...
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bioRxiv - Cell Biology 2020Quote: ... The concentrations of IgG in cell lysates and supernatants after recycling were measured with an IgG Human ELISA Kit (88-50550, Invitrogen), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: The supernatant from this homogenization step was used to evaluate levels of TNFα using the mouse TNF alpha High Sensitivity ELISA Kit according to manufacturer’s instructions (Invitrogen, BMS607HS). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Aβ concentrations in three separate fractions of brain homogenates were determined by Aβ42 and Aβ40 ELISA kits (both from Thermo Fisher Scientific). Results were normalized on the basis of the sample’s protein concentration.
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bioRxiv - Immunology 2021Quote: Enzyme-linked immunosorbent assay (ELISA) was performed for IFNγ and IL-2 using uncoated kits (respectively 88-7316-88 and 88-7025-88, ThermoFisher) following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and IL-1β in all of samples were determined using the appropriate ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufactures’ protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting supernatant was decanted and the concentration of secreted IL6 was measured by the human IL6 ELISA kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2021Quote: Cytokine levels in sampled medium supernatants were measured using commercially available ELISA kits in a blinded fashion and by following the manufacturer’s directions (Invitrogen, USA) for the following cytokines ...
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bioRxiv - Bioengineering 2021Quote: ... its expression was assessed in the conditioned media of both mono- and co-cultures at differentiation day 14 using an ELISA kit (ERIGF1, ThermoFisher), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The supernatants were collected after cytotoxicity assay for cytokine analysis by using IFN-γ and IL-2 ELISA kits (Invitrogen). Cytokine levels were analyzed by comparing the values obtained from samples to a standard curve generated from known concentrations of the cytokines.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...