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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... E40500) to stop the reaction and the absorbance was measured at 450/620 nm using an ELISA plate reader (Thermo Fisher, Varioskan LUX). The mean OD (optical density ...
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bioRxiv - Molecular Biology 2020Quote: ... An SPE plate (SOLAμTM HRP 2mg/1 mL 96 well plate, ThermoFisher Scientific, Germany) was put on a positive pressure manifold and conditioned with 200 μL methanol and 200 μL water with 0.1% formic acid (FA) ...
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bioRxiv - Plant Biology 2020Quote: ... GSTU7-V5 fusion was visualized with a 1:1 mix of SuperSignalTM West Pico and SuperSignalTM West Femto chemiluminescent substrates (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2019Quote: ... Cytokine measurement on tissue homogenate was assessed using ELISA (Mouse IFN gamma Uncoated ELISA (Invitrogen, #88-7314); Mouse TNF alpha Uncoated ELISA (Invitrogen ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Immunology 2021Quote: ... mouse uncoated ELISA kit (Catalog no.88-50400-22) and IgM mouse uncoated ELISA kit (Catalog no.88-50470-22) were from Thermo Fisher (Winnipeg, MB). Mouse IL-10 DuoSet ELISA (Catalog no ...
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bioRxiv - Biophysics 2021Quote: ... HRP-conjugate goat-anti-rabbit secondary antibody was used (1:15,000 dilution, Pierce) and developed with SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Davids Biotechnologie antibody (1:20,000 dilution detects 100 ng pure EspA in 1:20,000 dilution after 10 minutes exposure with SuperSignal West Pico PLUS Chemiluminescent Substrate from Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... and then incubated for 1 min with Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific). Bands were detected on a BioRad Chemidoc MP imaging system ...
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bioRxiv - Cancer Biology 2019Quote: ... membranes were incubated for 1 minute at room temperature with SuperSignal West Pico PLUS Chemiluminescent Substrate (34580, Thermo Fisher Scientific), and the bound antigen-antibody complexes were visualized using Odyssey Fc Imaging System (LI-COR Corp. ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated for 1 minute at room temperature with SuperSignal West Pico PLUS Chemiluminescent Substrate (34580, Thermo Fisher Scientific) and the bound antigen-antibody complexes were visualized using Odyssey Fc Imaging System (LI-COR Corp. ...
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bioRxiv - Molecular Biology 2020Quote: ... The biotinylated DNA fragments were applied to the gel-shift assay by using the LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, US) and Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: The probes used for EMSA were 3’-biotin end-labeled by HuaGene (Shanghai, China) and EMSA was conducted using a LightShift Chemiluminescent EMSA kit (Thermo Fisher Scientific). Mutant probes and competition probe (20-fold and 500-fold unlabeled oligonucleotides ...
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bioRxiv - Immunology 2020Quote: ... Binding of the primary antibody was detected using peroxidase-conjugated secondary antibodies and a SuperSignal West Dura chemiluminescent substrate kit (Thermo Scientific, 34075) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... After that the blot was cross-linked via UV lamp at 254 nm for 10 minutes then processed according to the given protocol (Light Shift Chemiluminescent EMSA kit, Thermo fisher scientific). The blot was quantified by ImageJ software.
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed three times with TBST and the bands were visualized using a chemiluminescent kit for HRP (Thermo Scientific, 34075) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA synthesis and target specific pre-amplification was done using Cell Direct one-step qRT-PCR kit (Invitrogen 46-7201). Pre-amplification mastermix was added to each well ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was isolated according to the manufacturer’s instructions of the Phire Plant Direct PCR Kit (Thermos Fisher Scientific, F130WH) and homozygous lines of mutants were confirmed by PCR.
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bioRxiv - Neuroscience 2021Quote: mRNA was isolated from 100,000 to 400,000 sorted microglia or BEMs (CD45low/int/CD11b+ cells) using the Dynabeads mRNA DIRECT Purification Kit (Invitrogen #61011) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: hiPSC-derived neuron and astrocyte co-cultures were loaded with Fluo 4 direct (calcium assay kit by Thermo Fisher Scientific) for 30 minutes in 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... total RNA was extracted by phase separation and poly(A) selection was performed using Dynabeads mRNA DIRECT Purification Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was extracted by phase separation and poly(A) selection was performed using Dynabeads mRNA DIRECT Purification Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 100 ng of RNA from each sample was then reverse transcribed into cDNA using SSIV Cell Direct cDNA Synthesis kit (lot# 00916637, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The ligation products were then purified with the Dynabeads mRNA direct purification kit and reverse transcription was performed using Superscript III reverse transcriptase (Invitrogen) and random hexamer primer attached to reverse adaptor sequence (5’-AGACGTGTGCTCTTCCGATCNNNNNN-3’) ...
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bioRxiv - Molecular Biology 2023Quote: ... polyA-tailed RNA was isolated from 75 μg of total RNA using the Dynabeads mRNA direct purification kit (Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR products were then subjected to Sanger sequencing using the BigDye Direct Cycle sequencing kit followed by analysis using a SeqStudio genetic analyzer (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... Direct labelling of antibodies was done using a Zenon™ Alexa FluorTM 594 Rabbit IgG Labelling Kit (# Z25307, ThermoFisher Scientific). DAPI was added to the BS in a concentration 1 µg/ml in the last incubation step ...
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bioRxiv - Microbiology 2023Quote: ... Individual clones were screened for correct tagging using oligos P15/P16 with Phire tissue direct PCR Master kit (Thermo Fisher). The correct clones were further confirmed using HA antibodies (Roche ...
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bioRxiv - Genomics 2023Quote: ... or control wild-type tissue were PCR amplified using the diluted protocol of the Phire Animal Tissue Direct PCR Kit (ThermoFisher) and a pair of oligonucleotides (Forward ...
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bioRxiv - Biochemistry 2021Quote: ... plates were washed 4 times with TBS-T and proteins were detected by addition of SuperSignal™ ELISA Femto substrate (ThermoFisher cat. no. PI37074). Luminescence was read using a BMG POLARstar Omega plate reader or Biotek Neo2 microplate reader.
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bioRxiv - Pathology 2022Quote: ... The reaction was stopped with 3N H2SO4 (50 μL/well) and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa, Finland) [35].
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bioRxiv - Molecular Biology 2020Quote: ... incubated at RT for 10 min and the absorbance was recorded at 540 nm using a 96-well plate ELISA reader (Thermo Fisher, Rockford, IL, USA).57 The concentration of nitrite was calculated according to the standard curve generated from known concentrations of sodium nitrite dissolved in fresh culture media.
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bioRxiv - Microbiology 2021Quote: ... The bacteria were cultured at 37°C for 48 hours and the concentrations were measured at multiple time points by the ELISA plate reader (Multiskan Go, Thermo Scientific, Waltham, MA, USA) at the OD of 600nm.
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Peptide competitions were undertaken by incubating RAVV GP ΔMuc at 200 ng per a well in Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... and Super Signal West Pico Chemiluminescent Substrate (PI34080, Thermo Fisher Scientific) or Amersham ECL Select Western Blotting Detection Reagent (RPN2235 ...
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bioRxiv - Cell Biology 2020Quote: ... and visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific). Films were scanned ...
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bioRxiv - Cell Biology 2020Quote: ... and developed in SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... membranes were incubated with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) for 1 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Blots were developed using SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific) or AP-Juice Low Background (PJK GmbH ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were developed in chemiluminescent substrate (Supersignal West Pico Plus, Thermofisher) for 2 min ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with CDP-Star chemiluminescent substrate (Thermo Fisher, USA) for alkaline phosphatase chemiluminescence reaction and detected on Amersham HyperfilmTM ECL films (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... membranes were incubated in SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) at a ratio of 1 ml per 20 cm2 of membrane and images captured using a BioRad gel imager.
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bioRxiv - Neuroscience 2021Quote: ... Proteins were visualized using SuperSignal West Pico Plus Chemiluminescent Substrate (ThermoFisher) and analyzed using ImageJ software.
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bioRxiv - Cancer Biology 2020Quote: ... Chemiluminescent signals were detected with ECL™ prime (Thermo Fisher Scientific) using the Biorad ChemiDoc system ...
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bioRxiv - Neuroscience 2020Quote: ... We used Super Signal West Pico chemiluminescent substrate (Thermo Fisher Scientific) or Luminata Forte (Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... Chemiluminescence detection was performed with SuperSignal Dura chemiluminescent substrate (Thermo Scientific) and developed by a ChemiDoc MP chemiluminescent imager (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... SuperSignal® West Femto Maximum Sensitivity Chemiluminescent Substrate (Thermo Scientific, USA) was used for imaging.